death domain binding / positive regulation of translational termination / ribosome hibernation / positive regulation of translational elongation / ribosomal subunit / laminin receptor activity / translational elongation / 90S preribosome / translation elongation factor activity / protein-RNA complex assembly ...death domain binding / positive regulation of translational termination / ribosome hibernation / positive regulation of translational elongation / ribosomal subunit / laminin receptor activity / translational elongation / 90S preribosome / translation elongation factor activity / protein-RNA complex assembly / rough endoplasmic reticulum / laminin binding / translation regulator activity / translation repressor activity / translation initiation factor binding / translation initiation factor activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / negative regulation of autophagy / cellular response to amino acid starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / apoptotic signaling pathway / spindle / rRNA processing / kinase activity / ribosome biogenesis / large ribosomal subunit / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / mitochondrial inner membrane / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / centrosome / endoplasmic reticulum membrane / GTP binding / nucleolus / RNA binding / zinc ion binding / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能
DAP1/DAPL1 / Death-associated protein / Intracellular hyaluronan-binding protein 4, N-terminal domain / Intracellular hyaluronan-binding protein 4 N-terminal / Hyaluronan / mRNA binding family / RNA binding protein HABP4/SERBP1 / Ribosomal protein L14, KOW motif / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold ...DAP1/DAPL1 / Death-associated protein / Intracellular hyaluronan-binding protein 4, N-terminal domain / Intracellular hyaluronan-binding protein 4 N-terminal / Hyaluronan / mRNA binding family / RNA binding protein HABP4/SERBP1 / Ribosomal protein L14, KOW motif / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / : / Translation initiation factor 5A-like, N-terminal / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Ribosomal protein S14, type Z, archaeal / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Elongation Factor G, domain II / Elongation Factor G, domain III / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Ribosomal L15/L27a, N-terminal / Elongation factor G C-terminus / Ribosomal protein L28e / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / Ribosomal protein L23 / EF-G domain III/V-like / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S2, eukaryotic / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S17e, conserved site / : / Ribosomal protein S17e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein L44e 類似検索 - ドメイン・相同性
40S ribosomal protein S27 / Large ribosomal subunit protein uL13 / 40S ribosomal protein S29 / 60S ribosomal protein L10 / Small ribosomal subunit protein uS7 / Ribosomal_L18e/L15P domain-containing protein / Ribosomal protein L15 / Large ribosomal subunit protein eL14 / 60S ribosomal protein L32 / KOW domain-containing protein ...40S ribosomal protein S27 / Large ribosomal subunit protein uL13 / 40S ribosomal protein S29 / 60S ribosomal protein L10 / Small ribosomal subunit protein uS7 / Ribosomal_L18e/L15P domain-containing protein / Ribosomal protein L15 / Large ribosomal subunit protein eL14 / 60S ribosomal protein L32 / KOW domain-containing protein / 60S ribosomal protein L37a isoform X3 / Large ribosomal subunit protein uL23 / 60S ribosomal protein L8 / Large ribosomal subunit protein eL24 / HABP4_PAI-RBP1 domain-containing protein / 60S ribosomal protein L9 / Large ribosomal subunit protein eL34 / Ribosomal protein L37 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein eS19 / Death-associated protein-like 1.S / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS24 / Large ribosomal subunit protein uL4B / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL22 / Small ribosomal subunit protein eS10 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL29 / MGC116435 protein / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL36 / 60S ribosomal protein L36a isoform X1 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL28 / 60S ribosomal protein L29 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein eS21 / 40S ribosomal protein S26 / Small ribosomal subunit protein uS12 / 60S ribosomal protein L6 / 40S ribosomal protein S8 / 40S ribosomal protein S25 / 60S ribosomal protein L13 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL14 / 60S ribosomal protein L21 / 60S ribosomal protein L7a / Ubiquitin-ribosomal protein eL40 fusion protein / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein eL31 / 60S ribosomal protein L27 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL15 / 60S ribosomal protein L18a / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS2 / Eukaryotic translation initiation factor 5A-1 / Elongation factor 2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL19 / DNA-(apurinic or apyrimidinic site) lyase / 40S ribosomal protein S6 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS1A / 60S ribosomal protein L5-B / Small ribosomal subunit protein RACK1 類似検索 - 構成要素
ジャーナル: Nature / 年: 2023 タイトル: A molecular network of conserved factors keeps ribosomes dormant in the egg. 著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne ...著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne Kandolf / Tzi-Yang Lin / Karl Mechtler / Anton Meinhart / David Haselbach / Andrea Pauli / 要旨: Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron ...Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.