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TitleA molecular network of conserved factors keeps ribosomes dormant in the egg.
Journal, issue, pagesNature, Vol. 613, Issue 7945, Page 712-720, Year 2023
Publish dateJan 18, 2023
AuthorsFriederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne Kandolf / Tzi-Yang Lin / Karl Mechtler / Anton Meinhart / David Haselbach / Andrea Pauli /
PubMed AbstractRibosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron ...Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.
External linksNature / PubMed:36653451 / PubMed Central
MethodsEM (single particle)
Resolution2.3 - 3.2 Å
Structure data

13111

7oya

EMDB-13111, PDB-7oya:
Cryo-EM structure of the 1 hpf zebrafish embryo 80S ribosome
Method: EM (single particle) / Resolution: 3.2 Å

13112

7oyb

EMDB-13112, PDB-7oyb:
Cryo-EM structure of the 6 hpf zebrafish embryo 80S ribosome
Method: EM (single particle) / Resolution: 2.4 Å

13113

7oyc

EMDB-13113, PDB-7oyc:
Cryo-EM structure of the Xenopus egg 80S ribosome
Method: EM (single particle) / Resolution: 2.4 Å

13114

7oyd

EMDB-13114, PDB-7oyd:
Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-13115: Cryo-EM structure of the 80S rabbit ribosome from reticulocyte lysate
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-14588: Cryo-EM map of the Xenopus egg dormant ribosome
Method: EM (single particle) / Resolution: 2.97 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • danio rerio (zebrafish)
  • xenopus laevis (African clawed frog)
  • oryctolagus cuniculus (rabbit)
KeywordsRIBOSOME / translation / embryo / zebrafish / maternal / Dap1 / Dap1b / Habp4 / Eif5a / Eef2b / Eef2 / DapL1 / egg / Xenopus

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