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基本情報
登録情報 | データベース: SASBDB / ID: SASDBE4 |
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![]() | Plakin domain of Human desmoplakin
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機能・相同性 | ![]() bundle of His cell-Purkinje myocyte adhesion involved in cell communication / cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication / desmosome organization / Keratinization / protein localization to cell-cell junction / ventricular compact myocardium morphogenesis / desmosome / intermediate filament organization / epithelial cell-cell adhesion / intermediate filament cytoskeleton organization ...bundle of His cell-Purkinje myocyte adhesion involved in cell communication / cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication / desmosome organization / Keratinization / protein localization to cell-cell junction / ventricular compact myocardium morphogenesis / desmosome / intermediate filament organization / epithelial cell-cell adhesion / intermediate filament cytoskeleton organization / Formation of the cornified envelope / peptide cross-linking / fascia adherens / cornified envelope / regulation of ventricular cardiac muscle cell action potential / Apoptotic cleavage of cell adhesion proteins / adherens junction organization / RND1 GTPase cycle / RND3 GTPase cycle / intermediate filament / skin development / regulation of heart rate by cardiac conduction / ficolin-1-rich granule membrane / intercalated disc / epidermis development / keratinocyte differentiation / adherens junction / protein kinase C binding / wound healing / structural constituent of cytoskeleton / cell-cell adhesion / scaffold protein binding / basolateral plasma membrane / Neutrophil degranulation / structural molecule activity / RNA binding / extracellular exosome / nucleus / plasma membrane / cytoplasm 類似検索 - 分子機能 |
生物種 | ![]() |
![]() | ![]() タイトル: The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. 著者: Esther Ortega / José A Manso / Rubén M Buey / Ana M Carballido / Arturo Carabias / Arnoud Sonnenberg / José M de Pereda / ![]() ![]() 要旨: Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins ...Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins contain a plakin domain formed by up to nine spectrin repeats (SR1-SR9) and an SH3 domain. The plakin domains of plectin and other plakins harbor binding sites for junctional proteins. We have combined x-ray crystallography with small angle x-ray scattering (SAXS) to elucidate the structure of the plakin domain of plectin, extending our previous analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 region allowed us to characterize its uniquely wide inter-repeat conformational variability. We also report the crystal structures of the SR7-SR8 region, refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region, which is conserved in all other plakin domains, forms a rigid segment stabilized by uniquely extensive inter-repeat contacts mediated by unusually long helices in SR8 and SR9. Using SAXS we show that in solution the SR3-SR6 and SR7-SR9 regions are rod-like segments and that SR3-SR9 of plectin has an extended shape with a small central kink. Other plakins, such as bullous pemphigoid antigen 1 and microtubule and actin cross-linking factor 1, are likely to have similar extended plakin domains. In contrast, desmoplakin has a two-segment structure with a central flexible hinge. The continuous versus segmented structures of the plakin domains of plectin and desmoplakin give insight into how different plakins might respond to tension and transmit mechanical signals. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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-Data source
SASBDBのページ | ![]() |
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-関連構造データ
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外部リンク
「今月の分子」の関連する項目 |
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-モデル
モデル #505 | ![]() タイプ: mix / ソフトウェア: EOM / ダミー原子の半径: 1.90 A / カイ2乗値: 3.192 / P-value: 0.000004 ![]() |
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モデル #506 | ![]() タイプ: mix / ソフトウェア: EOM / ダミー原子の半径: 1.90 A / カイ2乗値: 3.192 / P-value: 0.000004 ![]() |
モデル #507 | ![]() タイプ: mix / ソフトウェア: EOM / ダミー原子の半径: 1.90 A / カイ2乗値: 3.192 / P-value: 0.000004 ![]() |
モデル #508 | ![]() タイプ: mix / ソフトウェア: EOM / ダミー原子の半径: 1.90 A / カイ2乗値: 3.192 / P-value: 0.000004 ![]() |
モデル #509 | ![]() タイプ: mix / ソフトウェア: EOM / ダミー原子の半径: 1.90 A / カイ2乗値: 3.192 / P-value: 0.000004 ![]() |
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試料
![]() | 名称: Plakin domain of Human desmoplakin / 試料濃度: 0.59-4.70 |
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バッファ | 名称: sodium phosphate / 濃度: 20.00 mM / pH: 7.5 / 組成: 150 mM NaCl, 5% glycerol, 3 mM DTT |
要素 #323 | 名称: Desmoplakin / タイプ: protein / 記述: Plakin domain of Human Desmoplakin / 分子量: 98.68 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P15924 配列: GSHMWDEFTK HVTSECLGWM RQQRAEMDMV AWGVDLASVE QHINSHRGIH NSIGDYRWQL DKIKADLREK SAIYQLEEEY ENLLKASFER MDHLRQLQNI IQATSREIMW INDCEEEELL YDWSDKNTNI AQKQEAFSIR MSQLEVKEKE LNKLKQESDQ LVLNQHPASD ...配列: GSHMWDEFTK HVTSECLGWM RQQRAEMDMV AWGVDLASVE QHINSHRGIH NSIGDYRWQL DKIKADLREK SAIYQLEEEY ENLLKASFER MDHLRQLQNI IQATSREIMW INDCEEEELL YDWSDKNTNI AQKQEAFSIR MSQLEVKEKE LNKLKQESDQ LVLNQHPASD KIEAYMDTLQ TQWSWILQIT KCIDVHLKEN AAYFQFFEEA QSTEAYLKGL QDSIRKKYPC DKNMPLQHLL EQIKELEKER EKILEYKRQV QNLVNKSKKI VQLKPRNPDY RSNKPIILRA LCDYKQDQKI VHKGDECILK DNNERSKWYV TGPGGVDMLV PSVGLIIPPP NPLAVDLSCK IEQYYEAILA LWNQLYINMK SLVSWHYCMI DIEKIRAMTI AKLKTMRQED YMKTIADLEL HYQEFIRNSQ GSEMFGDDDK RKIQSQFTDA QKHYQTLVIQ LPGYPQHQTV TTTEITHHGT CQDVNHNKVI ETNRENDKQE TWMLMELQKI RRQIEHCEGR MTLKNLPLAD QGSSHHITVK INELKSVQND SQAIAEVLNQ LKDMLANFRG SEKYCYLQNE VFGLFQKLEN INGVTDGYLN SLCTVRALLQ AILQTEDMLK VYEARLTEEE TVCLDLDKVE AYRCGLKKIK NDLNLKKSLL ATMKTELQKA QQIHSQTSQQ YPLYDLDLGK FGEKVTQLTD RWQRIDKQID FRLWDLEKQI KQLRNYRDNY QAFCKWLYDA KRRQDSLESM KFGDSNTVMR FLNEQKNLHS EISGKRDKSE EVQKIAELCA NSIKDYELQL ASYTSGLETL LNIPIKRTMI QSPSGVILQE AADVHARYIE LLTRSGDYYR |
-実験情報
ビーム | 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany ![]() | ||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||
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距離分布関数 P(R) |
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結果 | コメント: The models displayed for this entry are selected representatives from a refined ensemble of conformations derived from ensemble optimization method (EOM). The Rg and Dmax distributions ...コメント: The models displayed for this entry are selected representatives from a refined ensemble of conformations derived from ensemble optimization method (EOM). The Rg and Dmax distributions of the ensemble and the additional results from EOM (that includes additional structural representatives) are available in the full-entry zip archive. The corresponding fit for this entry shows the final EOM fit to the SAXS data of the total refined ensemble.
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