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Yorodumi- SASDAF7: Metal-bound hETHE1 (Persulfide dioxygenase ETHE1, mitochondrial, ... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAF7 |
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Sample | Metal-bound hETHE1
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Function / homology | Function and homology information persulfide dioxygenase / hydrogen sulfide metabolic process / sulfur dioxygenase activity / Sulfide oxidation to sulfate / glutathione metabolic process / mitochondrial matrix / iron ion binding / mitochondrion / nucleoplasm / identical protein binding / cytoplasm Similarity search - Function |
Biological species | Homo sapiens (human) |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDAF7 |
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-Related structure data
Similar structure data |
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-External links
Related items in Molecule of the Month |
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-Models
Model #278 | Type: dummy / Software: DAMMIN (5.3) / Radius of dummy atoms: 1.80 A / Symmetry: P2 / Chi-square value: 1.308 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #287 | Type: atomic / Software: CRYSOL (2.8.3) / Chi-square value: 1.352 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Metal-bound hETHE1 / Specimen concentration: 2.00-5.00 |
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Buffer | Name: Tris / Concentration: 50.00 mM / pH: 8 Comment: TCEP does not reduce metals in solution, but prevents aggregation and precipitation of the protein Composition: 150 mM NaCl 2 mM TCEP |
Entity #157 | Name: hETHE1 / Type: protein / Description: Persulfide dioxygenase ETHE1, mitochondrial / Formula weight: 27.873 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: O95571 |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm | ||||||||||||||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M-W / Pixsize x: 0.172 mm | ||||||||||||||||||||||||||||||||||||||||||||||||
Scan | Title: Metal-bound hETHE1 / Measurement date: Oct 21, 2007 / Cell temperature: 9 °C / Exposure time: 30 sec. / Number of frames: 4 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 1369 /
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Result | Type of curve: merged Comments: Metal-bound hETHE1 was shown by SAXS to be a globular protein compatible with a dimeric hETHE1 in solution. The calculated scattering pattern from a high resolution model of dimeric ETHE1 ...Comments: Metal-bound hETHE1 was shown by SAXS to be a globular protein compatible with a dimeric hETHE1 in solution. The calculated scattering pattern from a high resolution model of dimeric ETHE1 is in a very good agreement (χ2 value of 1.5) with the experimental data from hETHE1. The low resolution shape of metal-bound hETHE1 was reconstructed ab initio using DAMMIN with P2 symmetry imposed. The ab initio model fits the experimental data with a discrepancy χ2 = 1.3 and is in a good agreement with the high resolution ETHE1 structure.
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