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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDEQ9 |
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![]() | Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP)
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機能・相同性 | ![]() cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / STING mediated induction of host immune responses / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / regulation of immunoglobulin production / cGAS/STING signaling pathway / pattern recognition receptor signaling pathway / regulation of T cell activation / cytoplasmic pattern recognition receptor signaling pathway ...cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / STING mediated induction of host immune responses / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / regulation of immunoglobulin production / cGAS/STING signaling pathway / pattern recognition receptor signaling pathway / regulation of T cell activation / cytoplasmic pattern recognition receptor signaling pathway / negative regulation of cGAS/STING signaling pathway / cGMP-mediated signaling / cellular response to exogenous dsRNA / positive regulation of type I interferon production / negative regulation of double-strand break repair via homologous recombination / nucleosome binding / positive regulation of defense response to virus by host / phosphatidylinositol-4,5-bisphosphate binding / activation of innate immune response / cAMP-mediated signaling / molecular condensate scaffold activity / determination of adult lifespan / positive regulation of cellular senescence / site of double-strand break / double-stranded DNA binding / defense response to virus / nuclear body / DNA repair / innate immune response / DNA damage response / chromatin binding / GTP binding / protein homodimerization activity / DNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 |
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![]() | ![]() タイトル: cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. 著者: Haipeng Liu / Pedro Moura-Alves / Gang Pei / Hans-Joachim Mollenkopf / Robert Hurwitz / Xiangyang Wu / Fei Wang / Siyu Liu / Mingtong Ma / Yiyan Fei / Chenggang Zhu / Anne-Britta Koehler / ...著者: Haipeng Liu / Pedro Moura-Alves / Gang Pei / Hans-Joachim Mollenkopf / Robert Hurwitz / Xiangyang Wu / Fei Wang / Siyu Liu / Mingtong Ma / Yiyan Fei / Chenggang Zhu / Anne-Britta Koehler / Dagmar Oberbeck-Mueller / Karin Hahnke / Marion Klemm / Ute Guhlich-Bornhof / Baoxue Ge / Anne Tuukkanen / Michael Kolbe / Anca Dorhoi / Stefan He Kaufmann / ![]() ![]() ![]() 要旨: Cyclic dinucleotides (CDNs) are important second messenger molecules in prokaryotes and eukaryotes. Within host cells, cytosolic CDNs are detected by STING and alert the host by activating innate ...Cyclic dinucleotides (CDNs) are important second messenger molecules in prokaryotes and eukaryotes. Within host cells, cytosolic CDNs are detected by STING and alert the host by activating innate immunity characterized by type I interferon (IFN) responses. Extracellular bacteria and dying cells can release CDNs, but sensing of extracellular CDNs (eCDNs) by mammalian cells remains elusive. Here, we report that endocytosis facilitates internalization of eCDNs. The DNA sensor cGAS facilitates sensing of endocytosed CDNs, their perinuclear accumulation, and subsequent STING-dependent release of type I IFN Internalized CDNs bind cGAS directly, leading to its dimerization, and the formation of a cGAS/STING complex, which may activate downstream signaling. Thus, eCDNs comprise microbe- and danger-associated molecular patterns that contribute to host-microbe crosstalk during health and disease. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #2780 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.138 / P-value: 0.000013 ![]() |
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モデル #2781 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.138 / P-value: 0.000013 ![]() |
モデル #2782 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.138 / P-value: 0.000013 ![]() |
モデル #2783 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.138 / P-value: 0.000013 ![]() |
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試料
![]() | 名称: Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP) 試料濃度: 9 mg/ml / Entity id: 1448 / 1449 |
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バッファ | 名称: 20 mM HEPES / pH: 7.4 |
要素 #1448 | 名称: cGAS / タイプ: protein / 記述: Cyclic GMP-AMP synthase / 分子量: 61.419 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q8N884 配列: MQPWHGKAMQ RASEAGATAP KASARNARGA PMDPTESPAA PEAALPKAGK FGPARKSGSR QKKSAPDTQE RPPVRATGAR AKKAPQRAQD TQPSDATSAP GAEGLEPPAA REPALSRAGS CRQRGARCST KPRPPPGPWD VPSPGLPVSA PILVRRDAAP GASKLRAVLE ...配列: MQPWHGKAMQ RASEAGATAP KASARNARGA PMDPTESPAA PEAALPKAGK FGPARKSGSR QKKSAPDTQE RPPVRATGAR AKKAPQRAQD TQPSDATSAP GAEGLEPPAA REPALSRAGS CRQRGARCST KPRPPPGPWD VPSPGLPVSA PILVRRDAAP GASKLRAVLE KLKLSRDDIS TAAGMVKGVV DHLLLRLKCD SAFRGVGLLN TGSYYEHVKI SAPNEFDVMF KLEVPRIQLE EYSNTRAYYF VKFKRNPKEN PLSQFLEGEI LSASKMLSKF RKIIKEEIND IKDTDVIMKR KRGGSPAVTL LISEKISVDI TLALESKSSW PASTQEGLRI QNWLSAKVRK QLRLKPFYLV PKHAKEGNGF QEETWRLSFS HIEKEILNNH GKSKTCCENK EEKCCRKDCL KLMKYLLEQL KERFKDKKHL DKFSSYHVKT AFFHVCTQNP QDSQWDRKDL GLCFDNCVTY FLQCLRTEKL ENYFIPEFNL FSSNLIDKRS KEFLTKQIEY ERNNEFPVFD EFLEYPYDVP DYAAAALEHH HHHH |
要素 #1449 | 名称: 2′3′-cGAMP / タイプ: other 記述: 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine 分子量: 0.674 / 分子数: 2 配列: C20H24N10O 13P2 |
-実験情報
ビーム | 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany ![]() | ||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年4月25日 / 保管温度: 10 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 3600 / 単位: 1/nm /
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距離分布関数 P(R) |
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結果 |
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