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- SASDES6: The 11S subunit of the Plasmodium falciparum proteasome, PA28 -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDES6
SampleThe 11S subunit of the Plasmodium falciparum proteasome, PA28
  • Proteasome activator PA28 (protein), PA28, Plasmodium falciparum (isolate 3D7)
Function / homology
Function and homology information


Proteasome assembly / proteasome activator complex / regulation of G1/S transition of mitotic cell cycle / endopeptidase activator activity / regulation of proteasomal protein catabolic process / nucleoplasm / cytoplasm
Similarity search - Function
Proteasome activator PA28 / Proteasome activator PA28, C-terminal domain / Proteasome activator superfamily / Proteasome activator PA28, C-terminal domain superfamily / Proteasome activator PA28, C-terminal
Similarity search - Domain/homology
Proteasome activator 28 subunit beta, putative
Similarity search - Component
Biological speciesPlasmodium falciparum (isolate 3D7) (eukaryote)
CitationDate: 2019 Aug 5
Title: The structure of the PA28–20S proteasome complex from Plasmodium falciparum and implications for proteostasis
Authors: Xie S / Metcalfe R / Hanssen E / Yang T / Gillett D / Leis A / Morton C / Kuiper M / Parker M / Spillman N / Wong W / Tsu C / Dick L / Griffin M
Contact author
  • Riley D. Metcalfe (Bio21 Institute, The University of Melbourne, Melbourne, Australia)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2502
Type: mix / Software: (CORAL05) / Radius of dummy atoms: 1.90 A / Symmetry: P7
Comment: Model of the flexible regions of PfPA28. P7 symmetry in core and flexible regions.
Chi-square value: 5.56
Search similar-shape structures of this assembly by Omokage search (details)
Model #2501
Type: mix / Software: (CORAL05) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Model of the flexible regions of PfPA28. P1 symmetry in flexible regions, P7 in core.
Chi-square value: 4.69
Search similar-shape structures of this assembly by Omokage search (details)
Model #2498
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P7 / Chi-square value: 1.87 / P-value: 0.000023
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: The 11S subunit of the Plasmodium falciparum proteasome, PA28
Specimen concentration: 10 mg/ml
BufferName: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide
pH: 7.4
Entity #1333Name: PA28 / Type: protein / Description: Proteasome activator PA28 / Formula weight: 33.182 / Num. of mol.: 7 / Source: Plasmodium falciparum (isolate 3D7) / References: UniProt: Q8I374
Sequence: GMEAYLNKID KIEPSDQKIK EEYNKFKYDI TKQAIESLRE RIPKRIIFFN NLVNVNSEPG SILNVNDLDG VSYKYKINKI EENVRKKHKG NNSSDFDERE KKKDSLDGHV KHFSNNEDSK LIIDDKVLYT HYVPSHKQIY LELEKIKTYA SELIEIIGNI KLWIQLNVPR ...Sequence:
GMEAYLNKID KIEPSDQKIK EEYNKFKYDI TKQAIESLRE RIPKRIIFFN NLVNVNSEPG SILNVNDLDG VSYKYKINKI EENVRKKHKG NNSSDFDERE KKKDSLDGHV KHFSNNEDSK LIIDDKVLYT HYVPSHKQIY LELEKIKTYA SELIEIIGNI KLWIQLNVPR IEDGNNFGVG IQEEAIQELA RVEESAFNLY DAIVKYYMER AKISTKVLKY PNVSDYQEAV RELDEKEWIH IKITIVDMRN NYIMLYDLLY KNWEKVVKPK NEDAHHRMTF

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Experimental information

BeamInstrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.682 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: The 11S subunit of the Plasmodium falciparum proteasome, PA28
Measurement date: Dec 2, 2017 / Cell temperature: 22 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/A /
MinMax
Q0.0053 0.3356
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 451 /
MinMax
Q0.009555 0.184991
P(R) point1 451
R0 129
Result
Type of curve: sec /
ExperimentalPorod
MW263 kDa302 kDa
Volume-484 nm3

P(R)GuinierGuinier error
Forward scattering, I00.2657 0.27 0.00019
Radius of gyration, Rg4.25 nm4.326 nm0.005

MinMax
D-12.9
Guinier point12 64

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