SASDDE9: Synechocystis fluorescence recovery protein FRPcc in the pre-oxid...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDDE9

試料

Synechocystis fluorescence recovery protein FRPcc in the pre-oxidized state (CC mutant)

• Fluorescence recovery protein (protein), Synechocystis sp. (strain PCC 6803 / Kazusa)

• 機能・相同性: Fluorescence recovery protein / Photoprotection regulator fluorescence recovery protein / plasma membrane-derived thylakoid membrane / Fluorescence recovery protein
• 生物種: Synechocystis sp. (strain PCC 6803 / Kazusa) (バクテリア)
• 引用: ジャーナル: Nat Commun / 年: 2018; タイトル: OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.; 著者: Nikolai N Sluchanko / Yury B Slonimskiy / Evgeny A Shirshin / Marcus Moldenhauer / Thomas Friedrich / Eugene G Maksimov / ; 要旨: In cyanobacteria, high light photoactivates the orange carotenoid protein (OCP) that binds to antennae complexes, dissipating energy and preventing the destruction of the photosynthetic apparatus. At low light, OCP is efficiently deactivated by a poorly understood action of the dimeric fluorescence recovery protein (FRP). Here, we engineer FRP variants with defined oligomeric states and scrutinize their functional interaction with OCP. Complemented by disulfide trapping and chemical crosslinking, structural analysis in solution reveals the topology of metastable complexes of OCP and the FRP scaffold with different stoichiometries. Unable to tightly bind monomeric FRP, photoactivated OCP recruits dimeric FRP, which subsequently monomerizes giving 1:1 complexes. This could be facilitated by a transient OCP-2FRP-OCP complex formed via the two FRP head domains, significantly improving FRP efficiency at elevated OCP levels. By identifying key molecular interfaces, our findings may inspire the design of optically triggered systems transducing light signals into protein-protein interactions.

登録者

• Nikolai Sluchanko (A.N. Bach Institute of Biochemistry, Moscow, Russia)

モデル

• モデル #2147: ; タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.06 / P-value: 0.063614; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Synechocystis fluorescence recovery protein FRPcc in the pre-oxidized state (CC mutant); 試料濃度: 1.7 mg/ml
• バッファ: 名称: 20 mM Tris 150 mM NaCl 3% glycerol / pH: 7.6

要素 #1175

タイプ: protein / 記述: Fluorescence recovery protein / 分子量: 14.09 / 分子数: 2 / 由来: Synechocystis sp. (strain PCC 6803 / Kazusa) / 参照: UniProt: P74103
配列:

MRGSHHHHHH TDPATMLQTA EAPWSQAETQ SAHALFRKAY QRELDGLCAT VQAQASQCTQ IDDLWKLHDF LSAKRHEIDG KYDDRQSVII FVFAQLLKEG LVQAEELTFL AADKQSKIKA LARL

実験情報

• ビーム: 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.124 Å
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Synechocystis fluorescence recovery protein FRPcc in the pre-oxidized state (CC mutant)
測定日: 2017年9月1日 / 保管温度: 20 °C / セル温度: 10 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.1022	4.4376

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 959 /

	Min	Max
Q	0.1022	2.74593
P(R) point	1	959
R	0	13

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	27.2 kDa	27.2 kDa
体積	-	43.4 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.01736	0.0001	0.017	-
慣性半径, Rg	3.131 nm	0.04	2.91 nm	0.09

	Min	Max
D	-	13
Guinier point	1	125