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TitleOCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 3869, Year 2018
Publish dateSep 24, 2018
AuthorsNikolai N Sluchanko / Yury B Slonimskiy / Evgeny A Shirshin / Marcus Moldenhauer / Thomas Friedrich / Eugene G Maksimov /
PubMed AbstractIn cyanobacteria, high light photoactivates the orange carotenoid protein (OCP) that binds to antennae complexes, dissipating energy and preventing the destruction of the photosynthetic apparatus. At ...In cyanobacteria, high light photoactivates the orange carotenoid protein (OCP) that binds to antennae complexes, dissipating energy and preventing the destruction of the photosynthetic apparatus. At low light, OCP is efficiently deactivated by a poorly understood action of the dimeric fluorescence recovery protein (FRP). Here, we engineer FRP variants with defined oligomeric states and scrutinize their functional interaction with OCP. Complemented by disulfide trapping and chemical crosslinking, structural analysis in solution reveals the topology of metastable complexes of OCP and the FRP scaffold with different stoichiometries. Unable to tightly bind monomeric FRP, photoactivated OCP recruits dimeric FRP, which subsequently monomerizes giving 1:1 complexes. This could be facilitated by a transient OCP-2FRP-OCP complex formed via the two FRP head domains, significantly improving FRP efficiency at elevated OCP levels. By identifying key molecular interfaces, our findings may inspire the design of optically triggered systems transducing light signals into protein-protein interactions.
External linksNat Commun / PubMed:30250028 / PubMed Central
MethodsSAS (X-ray synchrotron)
Structure data

SASDDE9:
Synechocystis fluorescence recovery protein FRPcc in the pre-oxidized state (CC mutant)
Method: SAXS/SANS

SASDDF9:
Synechocystis orange carotenoid-binding protein devoid of the NTE (OCP-ΔNTE, orange form)
Method: SAXS/SANS

SASDDG9:
The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form)
Method: SAXS/SANS

Source
  • Synechocystis sp. (strain pcc 6803 / kazusa) (bacteria)

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