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- SASDA52: Alcohol dehydrogenase in PBS (Alcohol dehydrogenase 1, adh) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDA52
SampleAlcohol dehydrogenase in PBS
  • Alcohol dehydrogenase 1 (protein), adh, Saccharomyces cerevisiae
Function / homology
Function and homology information


methylglyoxal reductase (NADH) / amino acid catabolic process to alcohol via Ehrlich pathway / octanol dehydrogenase (NAD+) activity / methylglyoxal reductase (NADH) activity / glycolytic fermentation to ethanol / butanol dehydrogenase (NAD+) activity / NADH oxidation / melatonin binding / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase ...methylglyoxal reductase (NADH) / amino acid catabolic process to alcohol via Ehrlich pathway / octanol dehydrogenase (NAD+) activity / methylglyoxal reductase (NADH) activity / glycolytic fermentation to ethanol / butanol dehydrogenase (NAD+) activity / NADH oxidation / melatonin binding / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase / allyl-alcohol dehydrogenase / allyl-alcohol dehydrogenase activity / zinc ion binding / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
Alcohol dehydrogenase, zinc-type, conserved site / Zinc-containing alcohol dehydrogenases signature. / Alcohol dehydrogenase-like, C-terminal / Zinc-binding dehydrogenase / Polyketide synthase, enoylreductase domain / Enoylreductase / Alcohol dehydrogenase, N-terminal / Alcohol dehydrogenase GroES-like domain / GroES-like superfamily / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Alcohol dehydrogenase 1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Contact author
  • Darja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #198
Type: atomic / Chi-square value: 6.165289
Search similar-shape structures of this assembly by Omokage search (details)
Model #207
Type: dummy / Radius of dummy atoms: 1.90 A / Chi-square value: 8.844676
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Alcohol dehydrogenase in PBS / Ext coefficient: 13.3 / Sample MW: 150 kDa / Specimen concentration: 24.89 mg/ml / Concentration method: nanodrop
BufferName: PBS / PK: 7 / pH: 7.4
Entity #3Name: adh / Type: protein / Description: Alcohol dehydrogenase 1 / Formula weight: 36.85 / Num. of mol.: 4 / Source: Saccharomyces cerevisiae / References: UniProt: P00330
Sequence: MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA ...Sequence:
MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA GHWVAISGAA GGLGSLAVQY AKAMGYRVLG IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA HGVINVSVSE AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK

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Experimental information

BeamInstrument name: DORIS III EMBL X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Measurement date: Sep 20, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 8 / Unit: 1/nm /
MinMax
Q0.0871 6.0145
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 421 /
MinMax
Q0.2334 2.531
P(R) point54 474
R0 8.925
Result
Type of curve: single_conc / Standard: BSA
ExperimentalStandardPorod
MW79 kDa79 kDa111 kDa
Volume--178 nm3

P(R)Guinier
Forward scattering, I078.8 76.55
Radius of gyration, Rg3.22 nm3.16 nm

MinMax
D-8.93
Guinier point54 120

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