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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDDE6 |
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![]() | Photoreceptor sensory box 1 light-state (SB1-LOV-R66I)
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機能・相同性 | ![]() |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. 著者: Katrin Röllen / Joachim Granzin / Renu Batra-Safferling / Andreas Maximilian Stadler / ![]() 要旨: Light, oxygen, voltage (LOV) photoreceptors consist of conserved photo-responsive domains in bacteria, archaea, plants and fungi, and detect blue-light via a flavin cofactor. We investigated the blue- ...Light, oxygen, voltage (LOV) photoreceptors consist of conserved photo-responsive domains in bacteria, archaea, plants and fungi, and detect blue-light via a flavin cofactor. We investigated the blue-light induced conformational transition of the dimeric photoreceptor PpSB1-LOV-R66I from Pseudomonas putida in solution by using small-angle X-ray scattering (SAXS). SAXS experiments of the fully populated light- and dark-states under steady-state conditions revealed significant structural differences between the two states that are in agreement with the known structures determined by crystallography. We followed the transition from the light- to the dark-state by using SAXS measurements in real-time. A two-state model based on the light- and dark-state conformations could describe the measured time-course SAXS data with a relaxation time τREC of ~ 34 to 35 min being larger than the recovery time found with UV/vis spectroscopy. Unlike the flavin chromophore-based UV/vis method that is sensitive to the local chromophore environment in flavoproteins, SAXS-based assay depends on protein conformational changes and provides with an alternative to measure the recovery kinetics. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #1970 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 1.891 ![]() |
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モデル #1971 | ![]() タイプ: mix / ソフトウェア: (2.8.3) / ダミー原子の半径: 1.90 A / カイ2乗値: 5.639 ![]() |
モデル #1972 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 5.639 ![]() |
モデル #1973 | ![]() タイプ: dummy / ソフトウェア: (2.8.3) / ダミー原子の半径: 2.00 A / 対称性 ![]() ![]() |
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試料
![]() | 名称: Photoreceptor sensory box 1 light-state (SB1-LOV-R66I) 試料濃度: 1.05-6.10 |
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バッファ | 名称: 10mM Tris, 10 mM NaCl / pH: 7 |
要素 #1060 | 名称: PpSB1-LOV-R66I light / タイプ: protein / 記述: Sensory box protein light-state (R66I) / 分子量: 18.588 / 分子数: 2 由来: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) 参照: UniProt: Q88E39 配列: MGSSHHHHHH SSGLVPRGSH MINAQLLQSM VDASNDGIVV AEKEGDDTIL IYVNAAFEYL TGYSRDEILY QDCRFLQGDD RDQLGRARIR KAMAEGRPCR EVLRNYRKDG SAFWNELSIT PVKSDFDQRT YFIGIQKDVS RQVELERELA ELRARPKPDE RA |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France ![]() ![]() | ||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||
スキャン | 測定日: 2014年12月2日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /
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距離分布関数 P(R) |
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