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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDAN5 |
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![]() | Cross-linked complex CytC_Adr
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Biological species | ![]() ![]() ![]() |
![]() | ![]() Title: Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy. Authors: Xingfu Xu / Wolfgang Reinle / Frank Hannemann / Peter V Konarev / Dmitri I Svergun / Rita Bernhardt / Marcellus Ubbink / ![]() Abstract: In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the ...In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the encounter state can represent a significant population of the complex. The redox proteins adrenodoxin (Adx) and cytochrome c (C c) associate to form such a weak and short-lived complex, which is nevertheless active in electron transfer. To study the conformational freedom within the protein complex, the native complex has been compared to a cross-linked counterpart by using solution scattering and NMR spectroscopy. Oligomerization behavior of the native complex in solution revealed by small-angle X-ray scattering indicates a stochastic nature of complex formation. For the cross-linked complex, interprotein paramagnetic effects are observed, whereas for the native complex, extensive averaging occurs, consistent with multiple orientations of the proteins within the complex. Simulations show that C c samples about half of the surface area of adrenodoxin. It is concluded that the complex of Adx/C c is entirely dynamic and can be considered as a pure encounter complex. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Models
Model #131 | ![]() Type: dummy / Software: Dammin / Radius of dummy atoms: 1.90 A / Chi-square value: 5.466244 ![]() |
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Model #132 | ![]() Type: atomic / Software: Sasref / Chi-square value: 3.4969 ![]() |
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Sample
![]() | Name: Cross-linked complex CytC_Adr / Sample MW: 22 kDa / Specimen concentration: 2.40-24.00 / Entity id: 108 / 109 |
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Buffer | Name: HEPES![]() |
Entity #108 | Name: Cyt_C / Type: protein / Description: Cytochrome C![]() Sequence: TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE |
Entity #109 | Name: Adrenodoxin![]() ![]() Sequence: KITVHFINRD GETLTTKGKI GDSLLDVVVQ NNLDIDGFGA CEGTLACSTC HLIFEQHIFE KLEEAITDEE NDMLDLAYGL TDDRSRLGCQ ICLTKAMDNM TVRVP |
-Experimental information
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Detector | Name: MAR 345 Image Plate | |||||||||||||||||||||
Scan |
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Distance distribution function P(R) |
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Result |
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