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- SASDCQ4: Collagenase ColH s2as2bs3 at pCa 5 (Collagenase ColH segement s2a... -

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Basic information

Entry
Database: SASBDB / ID: SASDCQ4
SampleCollagenase ColH s2as2bs3 at pCa 5
  • Collagenase ColH segement s2as2bs3 (protein), s2as2bs3, Hathewaya histolytica
Function / homology
Function and homology information


tripeptidase activity / microbial collagenase / collagen metabolic process / collagen binding / metalloendopeptidase activity / endopeptidase activity / calcium ion binding / proteolysis / zinc ion binding / extracellular region
Similarity search - Function
Collagenase ColG, catalytic helper subdomain / Collagenase G catalytic helper subdomain / Peptidase M9A/M9B, collagenase, bacterial / Peptidase M9, collagenase, N-terminal domain / Collagenase / Peptidase family M9 N-terminal / PKD domain / Peptidase, C-terminal, archaeal/bacterial / Bacterial pre-peptidase C-terminal domain / Polycystic kidney disease (PKD) domain profile. ...Collagenase ColG, catalytic helper subdomain / Collagenase G catalytic helper subdomain / Peptidase M9A/M9B, collagenase, bacterial / Peptidase M9, collagenase, N-terminal domain / Collagenase / Peptidase family M9 N-terminal / PKD domain / Peptidase, C-terminal, archaeal/bacterial / Bacterial pre-peptidase C-terminal domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / Neutral zinc metallopeptidases, zinc-binding region signature. / Concanavalin A-like lectin/glucanase domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Biological speciesHathewaya histolytica (bacteria)
Contact author
  • Christopher Ruth (University of Arkansas, Fayetteville, AR, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1292
Type: dummy / Software: (2.6.1) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 5.034
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Collagenase ColH s2as2bs3 at pCa 5 / Specimen concentration: 1.00-1.50
BufferName: 10mM HEPES 100mM NaCl 0.2mM EGTA / pH: 7.5
Entity #690Name: s2as2bs3 / Type: protein / Description: Collagenase ColH segement s2as2bs3 / Formula weight: 34.2 / Num. of mol.: 1 / Source: Hathewaya histolytica / References: UniProt: Q46085
Sequence: GSPGIPGPNE GDSKNSLPYG KINGTYKGTE KEKIKFSSEG SFDPDGKIVS YEWDFGDGNK SNEENPEHSY DKVGTYTVKL KVTDDKGESS VSTTTAEIKD LSENKLPVIY MHVPKSGALN QKVVFYGKGT YDPDGSIAGY QWDFGDGSDF SSEQNPSHVY TKKGEYTVTL ...Sequence:
GSPGIPGPNE GDSKNSLPYG KINGTYKGTE KEKIKFSSEG SFDPDGKIVS YEWDFGDGNK SNEENPEHSY DKVGTYTVKL KVTDDKGESS VSTTTAEIKD LSENKLPVIY MHVPKSGALN QKVVFYGKGT YDPDGSIAGY QWDFGDGSDF SSEQNPSHVY TKKGEYTVTL RVMDSSGQMS EKTMKIKITD PVYPIGTEKE PNNSKETASG PIVPGIPVSG TIENTSDQDY FYFDVITPGE VKIDINKLGY GGATWVVYDE NNNAVSYATD DGQNLSGKFK ADKPGRYYIH LYMFNGSYMP YRINIEGSVG R

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
Scan
Title: Collagenase ColH s2as2bs3 at pCa 5 / Measurement date: Oct 12, 2016 / Storage temperature: 4 °C / Cell temperature: 10 °C / Unit: 1/A /
MinMax
Q0.0125 0.5413
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 318 /
MinMax
Q0.024249 0.20095
P(R) point1 318
R0 142.1
Result
Type of curve: extrapolated
ExperimentalPorodEstimatedEstimated method
MW34.2 kDa---
Volume-36.8 nm358 DAMFILT

P(R)P(R) errorGuinierGuinier error
Forward scattering, I060.81 0.13 59.02 0.12
Radius of gyration, Rg3.6 nm0.01 3.32 nm0.08

MinMax
D-14.2
Guinier point22 46

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