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- PDB-9vpc: Cryo-EM structure of the IF1 bound bovine ATP synthase tetramer -

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Basic information

Entry
Database: PDB / ID: 9vpc
TitleCryo-EM structure of the IF1 bound bovine ATP synthase tetramer
Components
  • (ATP synthase ...) x 17
  • ATPase inhibitor, mitochondrial
KeywordsMEMBRANE PROTEIN / ATP synthase/hydrolase / oligomer / membrane bending / mammalian mitochondria
Function / homology
Function and homology information


negative regulation of mitochondrial ATP synthesis coupled proton transport / angiostatin binding / Formation of ATP by chemiosmotic coupling / Cristae formation / negative regulation of hydrolase activity / ATPase inhibitor activity / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial envelope / Mitochondrial translation termination / proton channel activity ...negative regulation of mitochondrial ATP synthesis coupled proton transport / angiostatin binding / Formation of ATP by chemiosmotic coupling / Cristae formation / negative regulation of hydrolase activity / ATPase inhibitor activity / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial envelope / Mitochondrial translation termination / proton channel activity / heme biosynthetic process / Mitochondrial protein degradation / negative regulation of endothelial cell proliferation / proton transmembrane transporter activity / proton motive force-driven ATP synthesis / proton-transporting two-sector ATPase complex, proton-transporting domain / proton motive force-driven mitochondrial ATP synthesis / MHC class I protein binding / positive regulation of blood vessel endothelial cell migration / H+-transporting two-sector ATPase / proton-transporting ATP synthase complex / proton-transporting ATP synthase activity, rotational mechanism / proton transmembrane transport / aerobic respiration / erythrocyte differentiation / ADP binding / mitochondrial membrane / ATPase binding / protein homotetramerization / calmodulin binding / mitochondrial inner membrane / lipid binding / structural molecule activity / cell surface / protein homodimerization activity / ATP hydrolysis activity / protein-containing complex / mitochondrion / ATP binding / metal ion binding / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
ATP synthase membrane subunit K / ATP synthase regulation / ATP synthase subunit ATP5MJ, mitochondrial / Mitochondrial proteolipid / ATP synthase, F0 complex, subunit G, mitochondrial / ATP synthase, F0 complex, subunit E, mitochondrial / Mitochondrial ATP synthase subunit g, animal / Mitochondrial ATP synthase g subunit / ATP synthase E chain / ATP synthase protein 8, metazoa ...ATP synthase membrane subunit K / ATP synthase regulation / ATP synthase subunit ATP5MJ, mitochondrial / Mitochondrial proteolipid / ATP synthase, F0 complex, subunit G, mitochondrial / ATP synthase, F0 complex, subunit E, mitochondrial / Mitochondrial ATP synthase subunit g, animal / Mitochondrial ATP synthase g subunit / ATP synthase E chain / ATP synthase protein 8, metazoa / Mitochondrial F1-F0 ATP synthase subunit F, predicted / ATP synthase protein 8, mammals / ATP synthase protein 8 / Mitochondrial F1F0-ATP synthase, subunit f / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / ATP synthase-coupling factor 6, mitochondrial / ATP synthase-coupling factor 6 superfamily, mitochondrial / Mitochondrial ATP synthase coupling factor 6 / : / Metazoan delta subunit of F1F0-ATP synthase, C-terminal domain / ATP synthase, F0 complex, subunit B/MI25 / ATP synthase, F0 complex, subunit B / Mitochondrial ATP synthase B chain precursor (ATP-synt_B) / ATP synthase, F0 complex, subunit D, mitochondrial / ATP synthase D chain, mitochondrial (ATP5H) / ATP synthase delta/epsilon subunit, C-terminal domain superfamily / ATP synthase, F0 complex, subunit D superfamily, mitochondrial / ATP synthase, F0 complex, subunit A, bacterial/mitochondria / ATP synthase, F1 complex, epsilon subunit, mitochondrial / ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial / Mitochondrial ATP synthase epsilon chain / ATPase, OSCP/delta subunit, conserved site / ATP synthase delta (OSCP) subunit signature. / F1F0 ATP synthase OSCP/delta subunit, N-terminal domain superfamily / ATP synthase, F0 complex, subunit A / ATP synthase, F0 complex, subunit A, active site / ATP synthase, F0 complex, subunit A superfamily / ATP synthase A chain / ATP synthase a subunit signature. / ATPase, OSCP/delta subunit / ATP synthase delta (OSCP) subunit / ATP synthase, F1 complex, delta/epsilon subunit / ATP synthase, F1 complex, delta/epsilon subunit, N-terminal / F0F1 ATP synthase delta/epsilon subunit, N-terminal / ATP synthase, Delta/Epsilon chain, beta-sandwich domain / ATP synthase, F0 complex, subunit C / F1F0 ATP synthase subunit C superfamily / ATP synthase, F0 complex, subunit C, DCCD-binding site / ATP synthase c subunit signature. / ATP synthase, F1 complex, gamma subunit conserved site / ATP synthase gamma subunit signature. / ATP synthase, F1 complex, beta subunit / ATP synthase, alpha subunit, C-terminal domain superfamily / : / ATP synthase, F1 complex, alpha subunit nucleotide-binding domain / ATP synthase, F1 complex, gamma subunit / ATP synthase, F1 complex, gamma subunit superfamily / ATP synthase / ATP synthase, alpha subunit, C-terminal / ATP synthase, F1 complex, alpha subunit / ATP synthase alpha/beta chain, C terminal domain / V-ATPase proteolipid subunit C-like domain / F/V-ATP synthase subunit C superfamily / ATP synthase subunit C / : / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / C-terminal domain of V and A type ATP synthase / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily / ATP synthase subunit alpha, N-terminal domain-like superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain / ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ATP synthase subunit alpha / ATP synthase F(1) complex catalytic subunit beta, mitochondrial / ATP synthase F(0) complex subunit a / ATPase inhibitor, mitochondrial / ATP synthase peripheral stalk subunit F6, mitochondrial / ATP synthase F(0) complex subunit 8 / ATP synthase F(1) complex subunit delta, mitochondrial / ATP synthase F(1) complex subunit gamma, mitochondrial / ATP synthase F(1) complex subunit epsilon, mitochondrial / ATP synthase F(0) complex subunit C2, mitochondrial ...ATP synthase subunit alpha / ATP synthase F(1) complex catalytic subunit beta, mitochondrial / ATP synthase F(0) complex subunit a / ATPase inhibitor, mitochondrial / ATP synthase peripheral stalk subunit F6, mitochondrial / ATP synthase F(0) complex subunit 8 / ATP synthase F(1) complex subunit delta, mitochondrial / ATP synthase F(1) complex subunit gamma, mitochondrial / ATP synthase F(1) complex subunit epsilon, mitochondrial / ATP synthase F(0) complex subunit C2, mitochondrial / ATP synthase peripheral stalk subunit b, mitochondrial / ATP synthase peripheral stalk subunit d, mitochondrial / ATP synthase peripheral stalk subunit OSCP, mitochondrial / ATP synthase F(0) complex subunit j, mitochondrial / ATP synthase F(0) complex subunit e, mitochondrial / ATP synthase F(0) complex subunit f, mitochondrial / ATP synthase F(0) complex subunit g, mitochondrial / ATP synthase F(0) complex subunit k, mitochondrial
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7.2 Å
AuthorsNakano, A. / Jiko, C. / Yamashita, E. / Yokoyama, K. / Gerle, C.
Funding support Japan, 2items
OrganizationGrant numberCountry
Japan Agency for Medical Research and Development (AMED)JP22ama121001j0004 Japan
Japan Society for the Promotion of Science (JSPS)20H03231 Japan
CitationJournal: Biorxiv / Year: 2025
Title: A planar dimer of bovine ATP synthase
Authors: Jiko, C. / Nakano, A. / Teshirogi, Y. / Yamashita, E. / Kurisu, G. / Standley, D. / Terada, T. / Mitsuoka, K. / Yokoyama, K. / Gerle, C.
History
DepositionJul 3, 2025Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 1, 2026Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
18: ATP synthase F(0) complex subunit 8
1A: ATP synthase subunit alpha
1B: ATP synthase subunit alpha
1C: ATP synthase subunit alpha
1D: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
1E: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
1F: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
1G: ATP synthase F(1) complex subunit gamma, mitochondrial
1H: ATP synthase F(1) complex subunit delta, mitochondrial
1I: ATP synthase F(1) complex subunit epsilon, mitochondrial
1J: ATPase inhibitor, mitochondrial
1K: ATP synthase F(0) complex subunit C2, mitochondrial
1L: ATP synthase F(0) complex subunit C2, mitochondrial
1M: ATP synthase F(0) complex subunit C2, mitochondrial
1N: ATP synthase F(0) complex subunit C2, mitochondrial
1O: ATP synthase F(0) complex subunit C2, mitochondrial
1P: ATP synthase F(0) complex subunit C2, mitochondrial
1Q: ATP synthase F(0) complex subunit C2, mitochondrial
1R: ATP synthase F(0) complex subunit C2, mitochondrial
1S: ATP synthase peripheral stalk subunit OSCP, mitochondrial
1a: ATP synthase F(0) complex subunit a
1b: ATP synthase peripheral stalk subunit b, mitochondrial
1d: ATP synthase peripheral stalk subunit d, mitochondrial
1e: ATP synthase F(0) complex subunit e, mitochondrial
1f: ATP synthase F(0) complex subunit f, mitochondrial
1g: ATP synthase F(0) complex subunit g, mitochondrial
1h: ATP synthase peripheral stalk subunit F6, mitochondrial
1j: ATP synthase F(0) complex subunit j, mitochondrial
1k: ATP synthase F(0) complex subunit k, mitochondrial
28: ATP synthase F(0) complex subunit 8
2A: ATP synthase subunit alpha
2B: ATP synthase subunit alpha
2C: ATP synthase subunit alpha
2D: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
2E: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
2F: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
2G: ATP synthase F(1) complex subunit gamma, mitochondrial
2H: ATP synthase F(1) complex subunit delta, mitochondrial
2I: ATP synthase F(1) complex subunit epsilon, mitochondrial
2J: ATPase inhibitor, mitochondrial
2K: ATP synthase F(0) complex subunit C2, mitochondrial
2L: ATP synthase F(0) complex subunit C2, mitochondrial
2M: ATP synthase F(0) complex subunit C2, mitochondrial
2N: ATP synthase F(0) complex subunit C2, mitochondrial
2O: ATP synthase F(0) complex subunit C2, mitochondrial
2P: ATP synthase F(0) complex subunit C2, mitochondrial
2Q: ATP synthase F(0) complex subunit C2, mitochondrial
2R: ATP synthase F(0) complex subunit C2, mitochondrial
2S: ATP synthase peripheral stalk subunit OSCP, mitochondrial
2a: ATP synthase F(0) complex subunit a
2b: ATP synthase peripheral stalk subunit b, mitochondrial
2d: ATP synthase peripheral stalk subunit d, mitochondrial
2e: ATP synthase F(0) complex subunit e, mitochondrial
2f: ATP synthase F(0) complex subunit f, mitochondrial
2g: ATP synthase F(0) complex subunit g, mitochondrial
2h: ATP synthase peripheral stalk subunit F6, mitochondrial
2j: ATP synthase F(0) complex subunit j, mitochondrial
2k: ATP synthase F(0) complex subunit k, mitochondrial
38: ATP synthase F(0) complex subunit 8
3A: ATP synthase subunit alpha
3B: ATP synthase subunit alpha
3C: ATP synthase subunit alpha
3D: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
3E: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
3F: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
3G: ATP synthase F(1) complex subunit gamma, mitochondrial
3H: ATP synthase F(1) complex subunit delta, mitochondrial
3I: ATP synthase F(1) complex subunit epsilon, mitochondrial
3J: ATPase inhibitor, mitochondrial
3K: ATP synthase F(0) complex subunit C2, mitochondrial
3L: ATP synthase F(0) complex subunit C2, mitochondrial
3M: ATP synthase F(0) complex subunit C2, mitochondrial
3N: ATP synthase F(0) complex subunit C2, mitochondrial
3O: ATP synthase F(0) complex subunit C2, mitochondrial
3P: ATP synthase F(0) complex subunit C2, mitochondrial
3Q: ATP synthase F(0) complex subunit C2, mitochondrial
3R: ATP synthase F(0) complex subunit C2, mitochondrial
3S: ATP synthase peripheral stalk subunit OSCP, mitochondrial
3a: ATP synthase F(0) complex subunit a
3b: ATP synthase peripheral stalk subunit b, mitochondrial
3d: ATP synthase peripheral stalk subunit d, mitochondrial
3e: ATP synthase F(0) complex subunit e, mitochondrial
3f: ATP synthase F(0) complex subunit f, mitochondrial
3g: ATP synthase F(0) complex subunit g, mitochondrial
3h: ATP synthase peripheral stalk subunit F6, mitochondrial
3j: ATP synthase F(0) complex subunit j, mitochondrial
3k: ATP synthase F(0) complex subunit k, mitochondrial
48: ATP synthase F(0) complex subunit 8
4A: ATP synthase subunit alpha
4B: ATP synthase subunit alpha
4C: ATP synthase subunit alpha
4D: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
4E: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
4F: ATP synthase F(1) complex catalytic subunit beta, mitochondrial
4G: ATP synthase F(1) complex subunit gamma, mitochondrial
4H: ATP synthase F(1) complex subunit delta, mitochondrial
4I: ATP synthase F(1) complex subunit epsilon, mitochondrial
4J: ATPase inhibitor, mitochondrial
4K: ATP synthase F(0) complex subunit C2, mitochondrial
4L: ATP synthase F(0) complex subunit C2, mitochondrial
4M: ATP synthase F(0) complex subunit C2, mitochondrial
4N: ATP synthase F(0) complex subunit C2, mitochondrial
4O: ATP synthase F(0) complex subunit C2, mitochondrial
4P: ATP synthase F(0) complex subunit C2, mitochondrial
4Q: ATP synthase F(0) complex subunit C2, mitochondrial
4R: ATP synthase F(0) complex subunit C2, mitochondrial
4S: ATP synthase peripheral stalk subunit OSCP, mitochondrial
4a: ATP synthase F(0) complex subunit a
4b: ATP synthase peripheral stalk subunit b, mitochondrial
4d: ATP synthase peripheral stalk subunit d, mitochondrial
4e: ATP synthase F(0) complex subunit e, mitochondrial
4f: ATP synthase F(0) complex subunit f, mitochondrial
4g: ATP synthase F(0) complex subunit g, mitochondrial
4h: ATP synthase peripheral stalk subunit F6, mitochondrial
4j: ATP synthase F(0) complex subunit j, mitochondrial
4k: ATP synthase F(0) complex subunit k, mitochondrial


Theoretical massNumber of molelcules
Total (without water)2,788,451116
Polymers2,788,451116
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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ATP synthase ... , 17 types, 112 molecules 182838481A1B1C2A2B2C3A3B3C4A4B4C1D1E1F2D2E2F3D3E3F4D4E4F1G2G...

#1: Protein
ATP synthase F(0) complex subunit 8 / A6L / F-ATPase subunit 8 / ATP synthase subunit A6L


Mass: 7944.523 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P03929
#2: Protein
ATP synthase subunit alpha


Mass: 59765.469 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: F1MLB8
#3: Protein
ATP synthase F(1) complex catalytic subunit beta, mitochondrial / ATP synthase subunit beta / ATP synthase F1 subunit beta


Mass: 56340.199 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle)
References: UniProt: P00829, H+-transporting two-sector ATPase
#4: Protein
ATP synthase F(1) complex subunit gamma, mitochondrial / ATP synthase subunit gamma / ATP synthase F1 subunit gamma / F-ATPase gamma subunit


Mass: 33119.035 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P05631
#5: Protein
ATP synthase F(1) complex subunit delta, mitochondrial / ATP synthase subunit delta / ATP synthase F1 subunit delta / F-ATPase delta subunit


Mass: 17626.992 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P05630
#6: Protein
ATP synthase F(1) complex subunit epsilon, mitochondrial / ATP synthase subunit epsilon / ATPase subunit epsilon / ATP synthase F1 subunit epsilon


Mass: 5793.889 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P05632
#8: Protein ...
ATP synthase F(0) complex subunit C2, mitochondrial / ATP synthase subunit c / ATP synthase lipid-binding protein / ATP synthase membrane subunit c locus ...ATP synthase subunit c / ATP synthase lipid-binding protein / ATP synthase membrane subunit c locus 2 / ATP synthase proteolipid P2 / ATPase protein 9 / ATPase subunit c


Mass: 15041.492 Da / Num. of mol.: 32 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P07926
#9: Protein
ATP synthase peripheral stalk subunit OSCP, mitochondrial / ATP synthase oligomycin sensitivity conferral protein (OSCP) / ATP synthase subunit O / Oligomycin ...ATP synthase oligomycin sensitivity conferral protein (OSCP) / ATP synthase subunit O / Oligomycin sensitivity conferral protein / OSCP


Mass: 23351.596 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P13621
#10: Protein
ATP synthase F(0) complex subunit a / ATP synthase subunit a / F-ATPase protein 6 / Proton-conducting channel / ATP synthase F(0) complex subunit a


Mass: 24801.785 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P00847
#11: Protein
ATP synthase peripheral stalk subunit b, mitochondrial / ATP synthase subunit b / ATP synthase F(0) complex subunit B1 / mitochondrial / ATP synthase ...ATP synthase subunit b / ATP synthase F(0) complex subunit B1 / mitochondrial / ATP synthase peripheral stalk-membrane subunit b / ATP synthase subunit b / ATPase subunit b


Mass: 28859.629 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P13619
#12: Protein
ATP synthase peripheral stalk subunit d, mitochondrial / ATP synthase subunit d / ATPase subunit d / ATP synthase peripheral stalk subunit d


Mass: 18719.453 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P13620
#13: Protein
ATP synthase F(0) complex subunit e, mitochondrial / ATP synthase subunit e / ATPase subunit e / ATP synthase membrane subunit e


Mass: 8336.688 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: Q00361
#14: Protein
ATP synthase F(0) complex subunit f, mitochondrial / ATP synthase subunit f / ATP synthase membrane subunit f


Mass: 10315.207 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: Q28851
#15: Protein
ATP synthase F(0) complex subunit g, mitochondrial / ATP synthase subunit g / ATPase subunit g / ATP synthase membrane subunit g


Mass: 11429.393 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: Q28852
#16: Protein
ATP synthase peripheral stalk subunit F6, mitochondrial / ATP synthase subunit F6 / ATPase subunit F6 / ATP synthase peripheral stalk subunit F6


Mass: 12549.321 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P02721
#17: Protein
ATP synthase F(0) complex subunit j, mitochondrial / ATP synthase subunit 6.8PL (subunit j) / 6.8 kDa mitochondrial proteolipid / 6.8 kDa mitochondrial ...ATP synthase subunit 6.8PL (subunit j) / 6.8 kDa mitochondrial proteolipid / 6.8 kDa mitochondrial proteolipid protein / MLQ


Mass: 6846.093 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P14790
#18: Protein
ATP synthase F(0) complex subunit k, mitochondrial / ATP synthase Diabetes-associated protein in insulin-sensitive tissues / ATP synthase membrane ...ATP synthase Diabetes-associated protein in insulin-sensitive tissues / ATP synthase membrane subunit DAPIT / mitochondrial / Diabetes-associated protein in insulin-sensitive tissues / Up-regulated during skeletal muscle growth protein 5


Mass: 6443.579 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: Q3ZBI7

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Protein , 1 types, 4 molecules 1J2J3J4J

#7: Protein
ATPase inhibitor, mitochondrial / ATPase inhibitory factor 1 (IF1) / ATP synthase F1 subunit epsilon / Inhibitor of F(1)F(o)-ATPase / ...ATPase inhibitory factor 1 (IF1) / ATP synthase F1 subunit epsilon / Inhibitor of F(1)F(o)-ATPase / IF(1) / IF1


Mass: 12326.715 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P01096

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Details

Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Bovine F-ATP synthase in the oligomeric form of an IF1 bound tetramer
Type: COMPLEX
Details: Oligomer fractions of bovine F-ATP synthase obtained from heart muscle tissue mitochondria.
Entity ID: all / Source: NATURAL
Molecular weightValue: 2.5 MDa / Experimental value: NO
Source (natural)Organism: Bos taurus (domestic cattle)
Buffer solutionpH: 7.3
SpecimenConc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameCategory
1Topazparticle selection
4CTFFINDCTF correction
7UCSF Chimeramodel fitting
8Cootmodel fitting
11RELIONfinal Euler assignment
12RELIONclassification
13RELION3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 7.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 39764 / Symmetry type: POINT
Atomic model buildingProtocol: FLEXIBLE FIT / Space: REAL
Atomic model buildingPDB-ID: 7AJD

7ajd


Accession code: 7AJD / Source name: PDB / Type: experimental model

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