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Open data
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Basic information
| Entry | Database: PDB / ID: 9rhi | |||||||||||||||||||||||||||
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| Title | pre-Initiation Complex on ARS1 DNA (monomer) | |||||||||||||||||||||||||||
Components |
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Keywords | REPLICATION / Macromolecular Complex DNA ATPase Helicase MCM2-7 | |||||||||||||||||||||||||||
| Function / homology | Function and homology informationregulation of mitotic DNA replication initiation / mating type switching / DNA-templated DNA replication maintenance of fidelity / Unwinding of DNA / DNA replication initiation / epsilon DNA polymerase complex / DNA strand elongation involved in mitotic DNA replication / MCM core complex / Assembly of the pre-replicative complex / Switching of origins to a post-replicative state ...regulation of mitotic DNA replication initiation / mating type switching / DNA-templated DNA replication maintenance of fidelity / Unwinding of DNA / DNA replication initiation / epsilon DNA polymerase complex / DNA strand elongation involved in mitotic DNA replication / MCM core complex / Assembly of the pre-replicative complex / Switching of origins to a post-replicative state / GINS complex / MCM complex binding / mitotic DNA replication preinitiation complex assembly / nuclear DNA replication / premeiotic DNA replication / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / Activation of the pre-replicative complex / mitotic DNA replication / DNA replication checkpoint signaling / nuclear pre-replicative complex / CMG complex / : / DNA replication preinitiation complex / Activation of ATR in response to replication stress / mitotic DNA replication checkpoint signaling / double-strand break repair via break-induced replication / MCM complex / mitotic DNA replication initiation / silent mating-type cassette heterochromatin formation / single-stranded DNA helicase activity / recombinational repair / regulation of DNA-templated DNA replication initiation / DNA strand elongation involved in DNA replication / mitotic G2 DNA damage checkpoint signaling / nuclear replication fork / Dual incision in TC-NER / DNA replication origin binding / chromosome, centromeric region / error-prone translesion synthesis / DNA replication initiation / protein kinase activator activity / subtelomeric heterochromatin formation / DNA polymerase binding / replication fork / DNA helicase activity / helicase activity / transcription elongation by RNA polymerase II / DNA-templated DNA replication / spindle pole / peroxisome / nuclear envelope / heterochromatin formation / single-stranded DNA binding / DNA helicase / chromosome, telomeric region / DNA replication / DNA damage response / chromatin binding / chromatin / endoplasmic reticulum / ATP hydrolysis activity / DNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / cytoplasm Similarity search - Function | |||||||||||||||||||||||||||
| Biological species | ![]() | |||||||||||||||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||||||||||||||||||||
Authors | Puehringer, T. / Butryn, A. / Couves, E.C. / Costa, A. | |||||||||||||||||||||||||||
| Funding support | United Kingdom, European Union, Germany, 4items
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Citation | Journal: To Be PublishedTitle: Structure of the pre-initiation complex explains CMG biogenesis Authors: Puehringer, T. / Canal, B. / Palm, G. / Butryn, A. / Couves, E.C. / Willhoft, O. / Lewis, J.S. / Diffley, J.F.X. / Costa, A. | |||||||||||||||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9rhi.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9rhi.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9rhi.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rh/9rhi ftp://data.pdbj.org/pub/pdb/validation_reports/rh/9rhi | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 53970MC ![]() 28vyC ![]() 9rhjC ![]() 9rhlC ![]() 9rhmC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
-DNA replication licensing factor ... , 5 types, 6 molecules 23467f
| #1: Protein | Mass: 98911.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MCM2, YBL023C, YBL0438 / Production host: ![]() |
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| #2: Protein | Mass: 111720.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: N-terminal CBP-tag with TEV protease cleavage site in frame with MCM3 Source: (gene. exp.) ![]() Gene: MCM3, YEL032W, SYGP-ORF23 / Production host: ![]() |
| #3: Protein | Mass: 105138.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MCM4, CDC54, HCD21, YPR019W, YP9531.13 / Production host: ![]() |
| #5: Protein | Mass: 113110.211 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MCM6, YGL201C / Production host: ![]() |
| #6: Protein | Mass: 95049.875 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MCM7, CDC47, YBR202W, YBR1441 / Production host: ![]() |
-Protein , 4 types, 4 molecules 5EFR
| #4: Protein | Mass: 86505.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MCM5, CDC46, YLR274W, L9328.1 / Production host: ![]() |
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| #11: Protein | Mass: 75154.703 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal ...Details: Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag,Internal 2xFLAG tag Source: (gene. exp.) ![]() Gene: CDC45, SLD4, YLR103C, L8004.11 / Production host: ![]() |
| #12: Protein | Mass: 78425.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: DPB2, YPR175W, P9705.7 / Production host: ![]() |
| #16: Protein | Mass: 29598.828 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: SLD7, YOR060C, YOR29-11 / Production host: ![]() |
-DNA chain , 2 types, 2 molecules AB
| #7: DNA chain | Mass: 9512.188 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
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| #8: DNA chain | Mass: 9547.166 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
-DNA replication complex GINS protein ... , 4 types, 4 molecules CDHI
| #9: Protein | Mass: 24437.859 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: PSF3, YOL146W / Production host: ![]() |
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| #10: Protein | Mass: 33983.617 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: SLD5, YDR489W / Production host: ![]() |
| #13: Protein | Mass: 24230.576 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: PSF1, YDR013W, PZA208, YD8119.18 / Production host: ![]() |
| #14: Protein | Mass: 25096.807 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: PSF2, YJL072C, HRF213, J1086 / Production host: ![]() |
-DNA replication regulator ... , 2 types, 3 molecules QTS
| #15: Protein | Mass: 81240.672 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Yeast Sld3 in frame with C-terminal TEV protease cleavage site and Twin-Strep-Tag Source: (gene. exp.) ![]() Gene: SLD3, YGL113W, G2980 / Production host: ![]() #17: Protein | | Mass: 91548.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Yeast Dpb11 in frame with C-terminal 3C protease cleavage site and 3xFLAG tag Source: (gene. exp.) ![]() Gene: DPB11, YJL090C, J0918 / Production host: ![]() |
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-Non-polymers , 3 types, 7 molecules 




| #18: Chemical | ChemComp-ZN / #19: Chemical | ChemComp-ADP / | #20: Chemical | ChemComp-MG / | |
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-Details
| Has ligand of interest | N |
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| Has protein modification | N |
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: Pre-initiation complex (Monomer) on replication origin (DNA) Type: COMPLEX / Entity ID: #1-#17 / Source: RECOMBINANT |
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| Molecular weight | Experimental value: NO |
| Source (natural) | Organism: ![]() |
| Source (recombinant) | Organism: ![]() |
| Buffer solution | pH: 7.5 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 2000 nm |
| Image recording | Electron dose: 39 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 2 / Num. of real images: 59347 |
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Processing
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
| Particle selection | Num. of particles selected: 1572464 | ||||||||||||||||||||||||
| 3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 302350 / Symmetry type: POINT | ||||||||||||||||||||||||
| Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
| Displacement parameters | Biso mean: 182.83 Å2 | ||||||||||||||||||||||||
| Refine LS restraints |
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About Yorodumi






United Kingdom, European Union,
Germany, 4items
Citation









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FIELD EMISSION GUN