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Open data
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Basic information
| Entry | Database: PDB / ID: 9n74 | |||||||||||||||
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| Title | SSU processome maturation and disassembly, State H | |||||||||||||||
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Keywords | RIBOSOME / SSU processome / ribosome assembly / RNA folding / RNA-protein interactions | |||||||||||||||
| Function / homology | Function and homology information18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / rRNA small subunit pseudouridine methyltransferase Nep1 / box H/ACA snoRNA binding ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / rRNA small subunit pseudouridine methyltransferase Nep1 / box H/ACA snoRNA binding / nuclear polyadenylation-dependent CUT catabolic process / RNA fragment catabolic process / U5 snRNA 3'-end processing / Noc4p-Nop14p complex / rRNA 2'-O-methylation / TRAMP-dependent tRNA surveillance pathway / exosome (RNase complex) / CURI complex / UTP-C complex / U4 snRNA 3'-end processing / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / t-UTP complex / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / nuclear microtubule / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / Mpp10 complex / nuclear exosome (RNase complex) / rRNA (pseudouridine) methyltransferase activity / rRNA modification / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / histone H2AQ104 methyltransferase activity / box C/D sno(s)RNA binding / regulation of rRNA processing / septum digestion after cytokinesis / tRNA re-export from nucleus / snRNA binding / rRNA (adenine-N6,N6-)-dimethyltransferase activity / histone mRNA catabolic process / RNA folding chaperone / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / rDNA heterochromatin / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear mRNA surveillance / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / box C/D methylation guide snoRNP complex / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / Negative regulators of DDX58/IFIH1 signaling / rRNA base methylation / U4 snRNA binding / O-methyltransferase activity / SUMOylation of RNA binding proteins / protein localization to nucleolus / 90S preribosome assembly / mTORC1-mediated signalling / rRNA methylation / Protein hydroxylation / RNA catabolic process / U4 snRNP / regulation of telomere maintenance / poly(U) RNA binding / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / preribosome, small subunit precursor / precatalytic spliceosome / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / spliceosomal complex assembly / positive regulation of transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / Ub-specific processing proteases / proteasome assembly / ribosomal subunit export from nucleus / RNA processing / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U4/U6 x U5 tri-snRNP complex Similarity search - Function | |||||||||||||||
| Biological species | ![]() | |||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.65 Å | |||||||||||||||
Authors | Buzovetsky, O. / Klinge, S. | |||||||||||||||
| Funding support | United States, 2items
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Citation | Journal: To Be PublishedTitle: Mechanism of helicase-mediated SSU processome maturation and disassembly Authors: Buzovetsky, O. / Klinge, S. | |||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9n74.cif.gz | 5.3 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9n74.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9n74.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Summary document | 9n74_validation.pdf.gz | 1.8 MB | Display | wwPDB validaton report |
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| Full document | 9n74_full_validation.pdf.gz | 1.9 MB | Display | |
| Data in XML | 9n74_validation.xml.gz | 554.3 KB | Display | |
| Data in CIF | 9n74_validation.cif.gz | 947.7 KB | Display | |
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n7/9n74 ftp://data.pdbj.org/pub/pdb/validation_reports/n7/9n74 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 49084MC ![]() 9n73C ![]() 9n7bC ![]() 48993 ![]() 48994 ![]() 48995 ![]() 48996 ![]() 48997 ![]() 48998 ![]() 48999 ![]() 49000 ![]() 49001 M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
+RNA chain , 3 types, 3 molecules L0L1L2
+40S ribosomal protein ... , 18 types, 18 molecules L3L4L5L6L7L8L9LCLDLELFLGNFNGNPNQOHSR
+Protein , 19 types, 22 molecules LHLOLUNBNDNLNMNSNVOAOUSCSDSESFSHSISJSKSVSWSZ
+U3 small nucleolar RNA-associated protein ... , 17 types, 17 molecules LILJLKLLLMLNLPLQLRLSLTLWNANHSPSSSY
+U3 small nucleolar ribonucleoprotein protein ... , 2 types, 2 molecules LZSM
+Ribosomal RNA-processing protein ... , 2 types, 2 molecules NISG
+Nucleolar protein ... , 2 types, 2 molecules SASB
+RRNA-processing protein ... , 2 types, 2 molecules SLSQ
+Nucleolar complex protein ... , 2 types, 2 molecules STSU
+Protein/peptide , 1 types, 1 molecules SX
+Non-polymers , 5 types, 45 molecules 








+Details
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: SSU processome maturation and disassembly, State H / Type: COMPLEX / Entity ID: #1-#68 / Source: NATURAL | ||||||||||||||||||||||||||||
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| Source (natural) | Organism: ![]() | ||||||||||||||||||||||||||||
| Buffer solution | pH: 7.5 | ||||||||||||||||||||||||||||
| Buffer component |
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| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 25000 nm / Nominal defocus min: 1000 nm |
| Image recording | Electron dose: 61.6 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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| 3D reconstruction | Resolution: 2.65 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 328554 / Symmetry type: POINT |
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United States, 2items
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FIELD EMISSION GUN