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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9n6z | |||||||||||||||
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| タイトル | SSU processome maturation and disassembly, State D | |||||||||||||||
要素 |
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キーワード | RIBOSOME / SSU processome / ribosome assembly / RNA folding / RNA-protein interactions | |||||||||||||||
| 機能・相同性 | 機能・相同性情報nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / tRNA wobble cytosine modification / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent mRNA catabolic process / nuclear polyadenylation-dependent CUT catabolic process / U5 snRNA 3'-end processing / tRNA cytidine N4-acetyltransferase activity ...nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / tRNA wobble cytosine modification / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent mRNA catabolic process / nuclear polyadenylation-dependent CUT catabolic process / U5 snRNA 3'-end processing / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / box H/ACA snoRNA binding / TRAMP-dependent tRNA surveillance pathway / RNA fragment catabolic process / tRNA acetylation / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / U4 snRNA 3'-end processing / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Noc4p-Nop14p complex / nuclear exosome (RNase complex) / t-UTP complex / nuclear microtubule / Pwp2p-containing subcomplex of 90S preribosome / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D sno(s)RNA binding / Mpp10 complex / rRNA modification / rRNA (pseudouridine) methyltransferase activity / snoRNA guided rRNA 2'-O-methylation / septum digestion after cytokinesis / histone H2AQ104 methyltransferase activity / rRNA 2'-O-methylation / snRNA binding / tRNA re-export from nucleus / histone mRNA catabolic process / nuclear mRNA surveillance / box C/D sno(s)RNA 3'-end processing / regulation of rRNA processing / rRNA methyltransferase activity / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / RNA folding chaperone / rDNA heterochromatin / U4/U6 snRNP / box C/D methylation guide snoRNP complex / tRNA export from nucleus / positive regulation of rRNA processing / single-stranded telomeric DNA binding / : / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / small nuclear ribonucleoprotein complex / O-methyltransferase activity / rRNA base methylation / SUMOylation of RNA binding proteins / protein localization to nucleolus / rRNA methylation / mTORC1-mediated signalling / U4 snRNA binding / Protein hydroxylation / RNA catabolic process / U4 snRNP / 90S preribosome assembly / regulation of telomere maintenance / U3 snoRNA binding / Formation of the ternary complex, and subsequently, the 43S complex / mRNA modification / poly(U) RNA binding / Translation initiation complex formation / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Ribosomal scanning and start codon recognition / poly(A)+ mRNA export from nucleus / precatalytic spliceosome / snoRNA binding / preribosome, small subunit precursor / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / positive regulation of transcription by RNA polymerase I / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / spliceosomal complex assembly / L13a-mediated translational silencing of Ceruloplasmin expression / nucleolar large rRNA transcription by RNA polymerase I / Ub-specific processing proteases / 90S preribosome / ribosomal subunit export from nucleus / RNA processing / U4/U6 x U5 tri-snRNP complex / proteasome assembly 類似検索 - 分子機能 | |||||||||||||||
| 生物種 | ![]() | |||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.74 Å | |||||||||||||||
データ登録者 | Buzovetsky, O. / Klinge, S. | |||||||||||||||
| 資金援助 | 米国, 2件
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引用 | ジャーナル: Nature / 年: 2025タイトル: Helicase-mediated mechanism of SSU processome maturation and disassembly. 著者: Olga Buzovetsky / Sebastian Klinge / ![]() 要旨: Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 small nucleolar RNA (snoRNA). The underlying molecular mechanisms ...Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 small nucleolar RNA (snoRNA). The underlying molecular mechanisms of SSU processome maturation, remodelling, disassembly and RNA quality control, and the transitions between states remain unknown owing to a paucity of intermediates. Here we report 16 native SSU processome structures alongside genetic data, revealing how two helicases, the Mtr4-exosome and Dhr1, are controlled for accurate and unidirectional ribosome biogenesis. Our data show how irreversible pre-ribosomal RNA degradation by the redundantly tethered RNA exosome couples the transformation of the SSU processome into a pre-40S particle, during which Utp14 can probe evolving surfaces, ultimately positioning and activating Dhr1 to unwind the U3 snoRNA and initiate nucleolar pre-40S release. This study highlights a paradigm for large dynamic RNA-protein complexes in which irreversible RNA degradation drives compositional changes and communicates these changes to govern enzyme activity while maintaining overall quality control. | |||||||||||||||
| 履歴 |
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9n6z.cif.gz | 4.4 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9n6z.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9n6z.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n6/9n6z ftp://data.pdbj.org/pub/pdb/validation_reports/n6/9n6z | HTTPS FTP |
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-関連構造データ
| 関連構造データ | ![]() 49079MC ![]() 9n6vC ![]() 9n6wC ![]() 9n6xC ![]() 9n6yC ![]() 9n70C ![]() 9n72C ![]() 9n73C ![]() 9n74C ![]() 9n75C ![]() 9n76C ![]() 9n77C ![]() 9n78C ![]() 9n79C ![]() 9n7aC ![]() 9n7bC C: 同じ文献を引用 ( M: このデータのモデリングに利用したマップデータ |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
+RNA鎖 , 3種, 3分子 L0L1L2
+40S ribosomal protein ... , 16種, 16分子 L3L4L5L6L7L8L9LCLDLELFLGNFNGNQSR
+タンパク質 , 19種, 23分子 LHLOLULXLYNBNDNENKNMNNNSNVOASCSDSESFSHSJSKSWSZ
+U3 small nucleolar RNA-associated protein ... , 17種, 17分子 LILJLKLLLMLNLPLQLRLSLTLWNANHSPSSSY
+Ribosome biogenesis protein ... , 2種, 2分子 LVSI
+U3 small nucleolar ribonucleoprotein protein ... , 3種, 3分子 LZNCSM
+Ribosomal RNA-processing protein ... , 2種, 2分子 NISG
+Nucleolar protein ... , 2種, 2分子 SASB
+RRNA-processing protein ... , 2種, 2分子 SLSQ
+Nucleolar complex protein ... , 2種, 2分子 STSU
+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: SSU processome maturation and disassembly, State D / タイプ: COMPLEX / Entity ID: all / 由来: NATURAL | ||||||||||||||||||||||||||||
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| 由来(天然) | 生物種: ![]() | ||||||||||||||||||||||||||||
| 緩衝液 | pH: 7.5 | ||||||||||||||||||||||||||||
| 緩衝液成分 |
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| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||||||
| 急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: TFS KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: OTHER |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 25000 nm / 最小 デフォーカス(公称値): 1000 nm |
| 撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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| 3次元再構成 | 解像度: 4.74 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 12826 / 対称性のタイプ: POINT |
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米国, 2件
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FIELD EMISSION GUN