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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9n6x | |||||||||||||||
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| タイトル | SSU processome maturation and disassembly, State B | |||||||||||||||
要素 |
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キーワード | RIBOSOME / SSU processome / ribosome assembly / RNA folding / RNA-protein interactions | |||||||||||||||
| 機能・相同性 | 機能・相同性情報tRNA wobble cytosine modification / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / regulation of ribosomal protein gene transcription by RNA polymerase II / tRNA acetylation / box H/ACA snoRNA binding / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex ...tRNA wobble cytosine modification / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / regulation of ribosomal protein gene transcription by RNA polymerase II / tRNA acetylation / box H/ACA snoRNA binding / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / rRNA 2'-O-methylation / Noc4p-Nop14p complex / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / t-UTP complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear microtubule / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / box C/D sno(s)RNA binding / snoRNA guided rRNA 2'-O-methylation / rRNA modification / histone H2AQ104 methyltransferase activity / septum digestion after cytokinesis / regulation of rRNA processing / tRNA re-export from nucleus / snRNA binding / RNA folding chaperone / box C/D sno(s)RNA 3'-end processing / rRNA methyltransferase activity / rDNA heterochromatin / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D methylation guide snoRNP complex / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / single-stranded telomeric DNA binding / rRNA primary transcript binding / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / Negative regulators of DDX58/IFIH1 signaling / rRNA base methylation / U4 snRNA binding / O-methyltransferase activity / SUMOylation of RNA binding proteins / protein localization to nucleolus / 90S preribosome assembly / mTORC1-mediated signalling / mRNA modification / rRNA methylation / Protein hydroxylation / U4 snRNP / poly(U) RNA binding / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / poly(A)+ mRNA export from nucleus / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / precatalytic spliceosome / preribosome, small subunit precursor / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / spliceosomal complex assembly / positive regulation of transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / Ub-specific processing proteases / proteasome assembly / RNA processing / regulation of translational fidelity / ribosomal subunit export from nucleus / U4/U6 x U5 tri-snRNP complex / ribosomal small subunit export from nucleus / vesicle-mediated transport / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / RNA endonuclease activity / nuclear periphery / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / maturation of SSU-rRNA / spliceosomal complex / small-subunit processome / translational initiation / enzyme activator activity / mRNA splicing, via spliceosome / maintenance of translational fidelity / rRNA processing 類似検索 - 分子機能 | |||||||||||||||
| 生物種 | ![]() | |||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.71 Å | |||||||||||||||
データ登録者 | Buzovetsky, O. / Klinge, S. | |||||||||||||||
| 資金援助 | 米国, 2件
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引用 | ジャーナル: Nature / 年: 2025タイトル: Helicase-mediated mechanism of SSU processome maturation and disassembly. 著者: Olga Buzovetsky / Sebastian Klinge / ![]() 要旨: Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 small nucleolar RNA (snoRNA). The underlying molecular mechanisms ...Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 small nucleolar RNA (snoRNA). The underlying molecular mechanisms of SSU processome maturation, remodelling, disassembly and RNA quality control, and the transitions between states remain unknown owing to a paucity of intermediates. Here we report 16 native SSU processome structures alongside genetic data, revealing how two helicases, the Mtr4-exosome and Dhr1, are controlled for accurate and unidirectional ribosome biogenesis. Our data show how irreversible pre-ribosomal RNA degradation by the redundantly tethered RNA exosome couples the transformation of the SSU processome into a pre-40S particle, during which Utp14 can probe evolving surfaces, ultimately positioning and activating Dhr1 to unwind the U3 snoRNA and initiate nucleolar pre-40S release. This study highlights a paradigm for large dynamic RNA-protein complexes in which irreversible RNA degradation drives compositional changes and communicates these changes to govern enzyme activity while maintaining overall quality control. | |||||||||||||||
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9n6x.cif.gz | 5.5 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9n6x.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9n6x.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| 文書・要旨 | 9n6x_validation.pdf.gz | 2 MB | 表示 | wwPDB検証レポート |
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| 文書・詳細版 | 9n6x_full_validation.pdf.gz | 2.2 MB | 表示 | |
| XML形式データ | 9n6x_validation.xml.gz | 627.1 KB | 表示 | |
| CIF形式データ | 9n6x_validation.cif.gz | 1023.7 KB | 表示 | |
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n6/9n6x ftp://data.pdbj.org/pub/pdb/validation_reports/n6/9n6x | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 49077MC ![]() 9n6vC ![]() 9n6wC ![]() 9n6yC ![]() 9n6zC ![]() 9n70C ![]() 9n73C ![]() 9n74C ![]() 9n75C ![]() 9n76C ![]() 9n77C ![]() 9n7aC C: 同じ文献を引用 ( M: このデータのモデリングに利用したマップデータ |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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要素
+RNA鎖 , 3種, 3分子 L0L1L2
+40S ribosomal protein ... , 16種, 16分子 L3L4L5L6L7L8L9LCLDLELFLGNFNGNQSR
+タンパク質 , 18種, 22分子 LHLOLULXLYNBNDNENKNMNNOASCSDSESFSHSJSKSVSWSZ
+U3 small nucleolar RNA-associated protein ... , 17種, 17分子 LILJLKLLLMLNLPLQLRLSLTLWNANHSPSSSY
+Ribosome biogenesis protein ... , 3種, 3分子 LVSISN
+U3 small nucleolar ribonucleoprotein protein ... , 3種, 3分子 LZNCSM
+Ribosomal RNA-processing protein ... , 2種, 2分子 NISG
+Nucleolar protein ... , 2種, 2分子 SASB
+RRNA-processing protein ... , 2種, 2分子 SLSQ
+Nucleolar complex protein ... , 2種, 2分子 STSU
+非ポリマー , 4種, 38分子 






+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: SSU processome maturation and disassembly, State B / タイプ: RIBOSOME / Entity ID: #1-#68 / 由来: NATURAL | |||||||||||||||||||||||||||||||||||
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| 由来(天然) | 生物種: ![]() | |||||||||||||||||||||||||||||||||||
| 緩衝液 | pH: 7.5 | |||||||||||||||||||||||||||||||||||
| 緩衝液成分 |
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| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | |||||||||||||||||||||||||||||||||||
| 急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: TFS KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: OTHER |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 25000 nm / 最小 デフォーカス(公称値): 1000 nm |
| 撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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| 3次元再構成 | 解像度: 3.71 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 28151 / 対称性のタイプ: POINT |
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万見について






米国, 2件
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FIELD EMISSION GUN