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- PDB-9h8b: Ex vivo cannulae fiber structure of Pyrodictium abyssi -

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Basic information

Entry
Database: PDB / ID: 9h8b
TitleEx vivo cannulae fiber structure of Pyrodictium abyssi
ComponentsCanX
KeywordsPROTEIN FIBRIL / Nanotube Archaea Helical protein fiber DNA-binding
Biological speciesPyrodictium abyssi DSM 6158 (archaea)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 2.3 Å
AuthorsSleutel, M. / Remaut, H.
Funding support Belgium, 1items
OrganizationGrant numberCountry
Research Foundation - Flanders (FWO)G043021N Belgium
Citation
Journal: Nat Commun / Year: 2025
Title: Donor strand complementation and calcium ion coordination drive the chaperone-free polymerization of archaeal cannulae.
Authors: Mike Sleutel / Ravi R Sonani / Jessalyn G Miller / Fengbin Wang / Andres Gonzalez Socorro / Yang Chen / Reece Martin / Borries Demeler / Michael J Rudolph / Vikram Alva / Han Remaut / Edward ...Authors: Mike Sleutel / Ravi R Sonani / Jessalyn G Miller / Fengbin Wang / Andres Gonzalez Socorro / Yang Chen / Reece Martin / Borries Demeler / Michael J Rudolph / Vikram Alva / Han Remaut / Edward H Egelman / Vincent P Conticello /
Abstract: Cannulae are structurally rigid tubular protein filaments that accumulate on the extracellular surface of archaea within the family Pyrodictiaceae during cell growth. These obligate anaerobes ...Cannulae are structurally rigid tubular protein filaments that accumulate on the extracellular surface of archaea within the family Pyrodictiaceae during cell growth. These obligate anaerobes propagate under hyperthermophilic conditions in which cannulae form a biomatrix that interconnects and sustains cells. The persistence of cannulae in this environment suggests that these filaments display significant thermostability, which has attracted technological interest in their development as synthetic protein-based biomaterials. Here, we report cryoEM structural analyses of ex vivo and in vitro assembled recombinant cannulae. We demonstrate that the interactions between protomers in native and recombinant cannulae is based on donor strand complementation (DSC), a form of non-covalent polymerization previously observed for bacterial chaperone-usher pili. Unexpectedly, calcium ion coordination at the subunit interfaces reinforces the network of donor strand interactions in the cannulae. This study provides insight into the mechanism of assembly of cannulae and the structural origin of their high stability and rigidity.
#1: Journal: bioRxiv / Year: 2024
Title: Donor Strand Complementation and Calcium Ion Coordination Drive the Chaperone-free Polymerization of Archaeal Cannulae.
Authors: Mike Sleutel / Ravi R Sonani / Jessalyn G Miller / Fengbin Wang / Andres Gonzalez Socorro / Yang Chen / Reece Martin / Borries Demeler / Michael J Rudolph / Vikram Alva / Han Remaut / Edward ...Authors: Mike Sleutel / Ravi R Sonani / Jessalyn G Miller / Fengbin Wang / Andres Gonzalez Socorro / Yang Chen / Reece Martin / Borries Demeler / Michael J Rudolph / Vikram Alva / Han Remaut / Edward H Egelman / Vincent P Conticello /
Abstract: Cannulae are tubular protein filaments that accumulate on the extracellular surface of the hyperthermophilic archaeon during cell division. Cannulae have been postulated to act as a primitive ...Cannulae are tubular protein filaments that accumulate on the extracellular surface of the hyperthermophilic archaeon during cell division. Cannulae have been postulated to act as a primitive extracellular matrix through which cells could communicate or exchange material, although their native biological function remains obscure. Here, we report cryoEM structural analyses of cannulae and of protein assemblies derived from recombinant cannula-like proteins. Three-dimensional reconstructions of cannulae revealed that the structural interactions between protomers in the native and recombinant filaments were based on donor strand complementation, a form of non-covalent polymerization in which a donor β-strand from one subunit is inserted into an acceptor groove in a β-sheet of a neighboring subunit. Donor strand complementation in cannulae is reinforced through calcium ion coordination at the interfaces between structural subunits in the respective assemblies. While donor strand complementation occurs during the assembly of chaperone-usher pili, this process requires the participation of accessory proteins that are localized in the outer membrane. In contrast, we demonstrate that calcium ions can induce assembly of cannulae in the absence of other co-factors. Crystallographic analysis of a recombinant cannula-like protein monomer provided evidence that calcium ion binding primes the precursor for donor strand invasion through unblocking of the acceptor groove. Bioinformatic analysis suggested that structurally homologous cannula-like proteins occurred within the genomes of other hyperthermophilic archaea and were encompassed within the TasA superfamily of biomatrix proteins. CryoEM structural analyses of tubular filaments derived from assembly of a recombinant cannula-like protein from an uncultured species revealed a common mode of assembly to the cannulae, in which donor strand complementation and calcium ion binding stabilized longitudinal and lateral assembly in tubular 2D sheets.
History
DepositionOct 28, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 18, 2024Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
Au: CanX
Av: CanX
Aw: CanX
Ax: CanX
Ay: CanX
Az: CanX
A1: CanX
A2: CanX
A3: CanX
A4: CanX
A5: CanX
A6: CanX
A7: CanX
A8: CanX
A9: CanX
A0: CanX
BA: CanX
BB: CanX
BC: CanX
BD: CanX
BE: CanX
BF: CanX
BG: CanX
BH: CanX
BI: CanX
BJ: CanX
BK: CanX
BL: CanX
BM: CanX
BN: CanX
BO: CanX
BP: CanX
BQ: CanX
BR: CanX
BS: CanX
BT: CanX
BU: CanX
BV: CanX
BW: CanX
BX: CanX
BY: CanX
BZ: CanX
Ba: CanX
Bb: CanX
Bc: CanX
Bd: CanX
Be: CanX
Bf: CanX
Bg: CanX
Bh: CanX
Bi: CanX
Bj: CanX
Bk: CanX
Bl: CanX
Bm: CanX
Bn: CanX
Bo: CanX
Bp: CanX
Bq: CanX
Br: CanX
Bs: CanX
Bt: CanX
Bu: CanX
Bv: CanX
Bw: CanX
Bx: CanX
By: CanX
Bz: CanX
B1: CanX
C9: CanX
DA: CanX
DC: CanX
DE: CanX
DG: CanX
DI: CanX
DK: CanX
DM: CanX
DO: CanX
DQ: CanX
DS: CanX
DU: CanX
DW: CanX
DY: CanX
Da: CanX
Dc: CanX
De: CanX
Dg: CanX
Di: CanX
Dk: CanX
Dm: CanX
Do: CanX
Dq: CanX
Ds: CanX
Du: CanX
Dw: CanX
Dy: CanX
D1: CanX
D3: CanX
D5: CanX
D7: CanX
D9: CanX
EA: CanX
EC: CanX
EE: CanX
EG: CanX
EI: CanX
EK: CanX
EM: CanX
EO: CanX
EQ: CanX
ES: CanX
EU: CanX
EW: CanX
EY: CanX
Ea: CanX
Ec: CanX
Ee: CanX
Eg: CanX
Ei: CanX
Ek: CanX
Em: CanX
Eo: CanX
Eq: CanX
Es: CanX
Eu: CanX
Ew: CanX
Ey: CanX
E1: CanX
E3: CanX
E5: CanX
E7: CanX
E9: CanX
FA: CanX
FC: CanX
FE: CanX
FG: CanX
FI: CanX
FK: CanX
FM: CanX
FO: CanX
FQ: CanX
FS: CanX
FU: CanX
FW: CanX
FY: CanX
Fa: CanX
Fc: CanX
Fe: CanX
Fg: CanX
Fi: CanX
Fk: CanX
Fm: CanX
Fo: CanX
Fq: CanX
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,939,375770
Polymers2,886,793154
Non-polymers52,582616
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails (eV)
d_1ens_1(chain "EE" and resid 1 through 149)
d_2ens_1(chain "A1" and resid 1 through 149)
d_3ens_1(chain "A2" and resid 1 through 149)
d_4ens_1(chain "A3" and resid 1 through 149)
d_5ens_1(chain "A4" and resid 1 through 149)
d_6ens_1(chain "A5" and resid 1 through 149)
d_7ens_1(chain "A6" and resid 1 through 149)
d_8ens_1(chain "A7" and resid 1 through 149)
d_9ens_1(chain "A8" and resid 1 through 149)
d_10ens_1(chain "A9" and resid 1 through 149)
d_11ens_1(chain "Au" and resid 1 through 149)
d_12ens_1(chain "Av" and resid 1 through 149)
d_13ens_1(chain "Aw" and resid 1 through 149)
d_14ens_1(chain "Ax" and resid 1 through 149)
d_15ens_1(chain "Ay" and resid 1 through 149)
d_16ens_1(chain "Az" and resid 1 through 149)
d_17ens_1(chain "B1" and resid 1 through 149)
d_18ens_1(chain "BA" and resid 1 through 149)
d_19ens_1(chain "BB" and resid 1 through 149)
d_20ens_1(chain "BC" and resid 1 through 149)
d_21ens_1(chain "BD" and resid 1 through 149)
d_22ens_1(chain "BE" and resid 1 through 149)
d_23ens_1(chain "BF" and resid 1 through 149)
d_24ens_1(chain "BG" and resid 1 through 149)
d_25ens_1(chain "BH" and resid 1 through 149)
d_26ens_1(chain "BI" and resid 1 through 149)
d_27ens_1(chain "BJ" and resid 1 through 149)
d_28ens_1(chain "BK" and resid 1 through 149)
d_29ens_1(chain "BL" and resid 1 through 149)
d_30ens_1(chain "BM" and resid 1 through 149)
d_31ens_1(chain "BN" and resid 1 through 149)
d_32ens_1(chain "BO" and resid 1 through 149)
d_33ens_1(chain "BP" and resid 1 through 149)
d_34ens_1(chain "BQ" and resid 1 through 149)
d_35ens_1(chain "BR" and resid 1 through 149)
d_36ens_1(chain "BS" and resid 1 through 149)
d_37ens_1(chain "BT" and resid 1 through 149)
d_38ens_1(chain "BU" and resid 1 through 149)
d_39ens_1(chain "BV" and resid 1 through 149)
d_40ens_1(chain "BW" and resid 1 through 149)
d_41ens_1(chain "BX" and resid 1 through 149)
d_42ens_1(chain "BY" and resid 1 through 149)
d_43ens_1(chain "BZ" and resid 1 through 149)
d_44ens_1(chain "Ba" and resid 1 through 149)
d_45ens_1(chain "Bb" and resid 1 through 149)
d_46ens_1(chain "Bc" and resid 1 through 149)
d_47ens_1(chain "Bd" and resid 1 through 149)
d_48ens_1(chain "Be" and resid 1 through 149)
d_49ens_1(chain "Bf" and resid 1 through 149)
d_50ens_1(chain "Bg" and resid 1 through 149)
d_51ens_1(chain "Bh" and resid 1 through 149)
d_52ens_1(chain "Bi" and resid 1 through 149)
d_53ens_1(chain "Bj" and resid 1 through 149)
d_54ens_1(chain "Bk" and resid 1 through 149)
d_55ens_1(chain "Bl" and resid 1 through 149)
d_56ens_1(chain "Bm" and resid 1 through 149)
d_57ens_1(chain "Bn" and resid 1 through 149)
d_58ens_1(chain "Bo" and resid 1 through 149)
d_59ens_1(chain "Bp" and resid 1 through 149)
d_60ens_1(chain "Bq" and resid 1 through 149)
d_61ens_1(chain "Br" and resid 1 through 149)
d_62ens_1(chain "Bs" and resid 1 through 149)
d_63ens_1(chain "Bt" and resid 1 through 149)
d_64ens_1(chain "Bu" and resid 1 through 149)
d_65ens_1(chain "Bv" and resid 1 through 149)
d_66ens_1(chain "Bw" and resid 1 through 149)
d_67ens_1(chain "Bx" and resid 1 through 149)
d_68ens_1(chain "By" and resid 1 through 149)
d_69ens_1(chain "Bz" and resid 1 through 149)
d_70ens_1(chain "C9" and resid 1 through 149)
d_71ens_1(chain "D1" and resid 1 through 149)
d_72ens_1(chain "D3" and resid 1 through 149)
d_73ens_1(chain "D5" and resid 1 through 149)
d_74ens_1(chain "D7" and resid 1 through 149)
d_75ens_1(chain "D9" and resid 1 through 149)
d_76ens_1(chain "DA" and resid 1 through 149)
d_77ens_1(chain "DC" and resid 1 through 149)
d_78ens_1(chain "DE" and resid 1 through 149)
d_79ens_1(chain "DG" and resid 1 through 149)
d_80ens_1(chain "DI" and resid 1 through 149)
d_81ens_1(chain "DK" and resid 1 through 149)
d_82ens_1(chain "DM" and resid 1 through 149)
d_83ens_1(chain "DO" and resid 1 through 149)
d_84ens_1(chain "DQ" and resid 1 through 149)
d_85ens_1(chain "DS" and resid 1 through 149)
d_86ens_1(chain "DU" and resid 1 through 149)
d_87ens_1(chain "DW" and resid 1 through 149)
d_88ens_1(chain "DY" and resid 1 through 149)
d_89ens_1(chain "Da" and resid 1 through 149)
d_90ens_1(chain "Dc" and resid 1 through 149)
d_91ens_1(chain "De" and resid 1 through 149)
d_92ens_1(chain "Dg" and resid 1 through 149)
d_93ens_1(chain "Di" and resid 1 through 149)
d_94ens_1(chain "Dk" and resid 1 through 149)
d_95ens_1(chain "Dm" and resid 1 through 149)
d_96ens_1(chain "Do" and resid 1 through 149)
d_97ens_1(chain "Dq" and resid 1 through 149)
d_98ens_1(chain "Ds" and resid 1 through 149)
d_99ens_1(chain "Du" and resid 1 through 149)
d_100ens_1(chain "Dw" and resid 1 through 149)
d_101ens_1(chain "Dy" and resid 1 through 149)
d_102ens_1(chain "E1" and resid 1 through 149)
d_103ens_1(chain "E3" and resid 1 through 149)
d_104ens_1(chain "E5" and resid 1 through 149)
d_105ens_1(chain "E7" and resid 1 through 149)
d_106ens_1(chain "E9" and resid 1 through 149)
d_107ens_1(chain "EA" and resid 1 through 149)
d_108ens_1(chain "EC" and resid 1 through 149)
d_109ens_1(chain "FE" and resid 1 through 149)
d_110ens_1(chain "EG" and resid 1 through 149)
d_111ens_1(chain "EI" and resid 1 through 149)
d_112ens_1(chain "EK" and resid 1 through 149)
d_113ens_1(chain "EM" and resid 1 through 149)
d_114ens_1(chain "EO" and resid 1 through 149)
d_115ens_1(chain "EQ" and resid 1 through 149)
d_116ens_1(chain "ES" and resid 1 through 149)
d_117ens_1(chain "EU" and resid 1 through 149)
d_118ens_1(chain "EW" and resid 1 through 149)
d_119ens_1(chain "EY" and resid 1 through 149)
d_120ens_1(chain "Ea" and resid 1 through 149)
d_121ens_1(chain "Ec" and resid 1 through 149)
d_122ens_1(chain "Ee" and resid 1 through 149)
d_123ens_1(chain "Eg" and resid 1 through 149)
d_124ens_1(chain "Ei" and resid 1 through 149)
d_125ens_1(chain "Ek" and resid 1 through 149)
d_126ens_1(chain "Em" and resid 1 through 149)
d_127ens_1(chain "Eo" and resid 1 through 149)
d_128ens_1(chain "Eq" and resid 1 through 149)
d_129ens_1(chain "Es" and resid 1 through 149)
d_130ens_1(chain "Eu" and resid 1 through 149)
d_131ens_1(chain "Ew" and resid 1 through 149)
d_132ens_1(chain "Ey" and resid 1 through 149)
d_133ens_1(chain "FA" and resid 1 through 149)
d_134ens_1(chain "A0" and resid 1 through 149)
d_135ens_1(chain "FC" and resid 1 through 149)
d_136ens_1(chain "FG" and resid 1 through 149)
d_137ens_1(chain "FI" and resid 1 through 149)
d_138ens_1(chain "FK" and resid 1 through 149)
d_139ens_1(chain "FM" and resid 1 through 149)
d_140ens_1(chain "FO" and resid 1 through 149)
d_141ens_1(chain "FQ" and resid 1 through 149)
d_142ens_1(chain "FS" and resid 1 through 149)
d_143ens_1(chain "FU" and resid 1 through 149)
d_144ens_1(chain "FW" and resid 1 through 149)
d_145ens_1(chain "FY" and resid 1 through 149)
d_146ens_1(chain "Fa" and resid 1 through 149)
d_147ens_1(chain "Fc" and resid 1 through 149)
d_148ens_1(chain "Fe" and resid 1 through 149)
d_149ens_1(chain "Fg" and resid 1 through 149)
d_150ens_1(chain "Fi" and resid 1 through 149)
d_151ens_1(chain "Fk" and resid 1 through 149)
d_152ens_1(chain "Fm" and resid 1 through 149)
d_153ens_1(chain "Fo" and resid 1 through 149)
d_154ens_1(chain "Fq" and resid 1 through 149)

NCS domain segments:

Component-ID: 1 / Ens-ID: ens_1 / Beg auth comp-ID: THR / Beg label comp-ID: THR / End auth comp-ID: SER / End label comp-ID: SER / Auth seq-ID: 1 - 149 / Label seq-ID: 26 - 174

Dom-IDAuth asym-IDLabel asym-ID
d_1EEZC
d_2A1G
d_3A2H
d_4A3I
d_5A4J
d_6A5K
d_7A6L
d_8A7M
d_9A8N
d_10A9O
d_11AuA
d_12AvB
d_13AwC
d_14AxD
d_15AyE
d_16AzF
d_17B1QB
d_18BAQ
d_19BBR
d_20BCS
d_21BDT
d_22BEU
d_23BFV
d_24BGW
d_25BHX
d_26BIY
d_27BJZ
d_28BKAA
d_29BLBA
d_30BMCA
d_31BNDA
d_32BOEA
d_33BPFA
d_34BQGA
d_35BRHA
d_36BSIA
d_37BTJA
d_38BUKA
d_39BVLA
d_40BWMA
d_41BXNA
d_42BYOA
d_43BZPA
d_44BaQA
d_45BbRA
d_46BcSA
d_47BdTA
d_48BeUA
d_49BfVA
d_50BgWA
d_51BhXA
d_52BiYA
d_53BjZA
d_54BkAB
d_55BlBB
d_56BmCB
d_57BnDB
d_58BoEB
d_59BpFB
d_60BqGB
d_61BrHB
d_62BsIB
d_63BtJB
d_64BuKB
d_65BvLB
d_66BwMB
d_67BxNB
d_68ByOB
d_69BzPB
d_70C9RB
d_71D1SC
d_72D3TC
d_73D5UC
d_74D7VC
d_75D9WC
d_76DASB
d_77DCTB
d_78DEUB
d_79DGVB
d_80DIWB
d_81DKXB
d_82DMYB
d_83DOZB
d_84DQAC
d_85DSBC
d_86DUCC
d_87DWDC
d_88DYEC
d_89DaFC
d_90DcGC
d_91DeHC
d_92DgIC
d_93DiJC
d_94DkKC
d_95DmLC
d_96DoMC
d_97DqNC
d_98DsOC
d_99DuPC
d_100DwQC
d_101DyRC
d_102E1XD
d_103E3YD
d_104E5ZD
d_105E7AE
d_106E9BE
d_107EAXC
d_108ECYC
d_109FEEE
d_110EGAD
d_111EIBD
d_112EKCD
d_113EMDD
d_114EOED
d_115EQFD
d_116ESGD
d_117EUHD
d_118EWID
d_119EYJD
d_120EaKD
d_121EcLD
d_122EeMD
d_123EgND
d_124EiOD
d_125EkPD
d_126EmQD
d_127EoRD
d_128EqSD
d_129EsTD
d_130EuUD
d_131EwVD
d_132EyWD
d_133FACE
d_134A0P
d_135FCDE
d_136FGFE
d_137FIGE
d_138FKHE
d_139FMIE
d_140FOJE
d_141FQKE
d_142FSLE
d_143FUME
d_144FWNE
d_145FYOE
d_146FaPE
d_147FcQE
d_148FeRE
d_149FgSE
d_150FiTE
d_151FkUE
d_152FmVE
d_153FoWE
d_154FqXE

NCS oper:
IDCodeMatrixVector
1given(-0.820431166487, -0.571745311335, 4.80356026832E-6), (0.571745311173, -0.820431166409, -1.83606622507E-5), (1.44386131087E-5, -1.23172464874E-5, 0.99999999982)509.531632017, 265.981084613, 38.1355085198
2given(-0.922011639454, -0.3871621581, 6.8208020897E-6), (0.387162157922, -0.922011639362, -1.87937417046E-5), (1.35650845136E-5, -1.46872921447E-5, 0.9999999998)491.850862048, 326.934049473, 41.3142650984
3given(-0.982664585099, -0.185392322155, 8.88246231415E-6), (0.185392321968, -0.982664584998, -1.86165405053E-5), (1.21798448185E-5, -1.66470747384E-5, 0.999999999787)461.791653533, 382.829893086, 44.4930046382
4given(-0.999697251066, 0.0246050013402, 1.09329500091E-5), (-0.0246050015251, -0.999697250975, -1.71129067623E-5), (1.05085769753E-5, -1.73767310996E-5, 0.999999999794)420.689145354, 431.186725116, 47.6714602778
5given(-0.972353899354, 0.233512085842, 1.32704191181E-5), (-0.23351208604, -0.972353899264, -1.61074820804E-5), (9.14225203613E-6, -1.87609762606E-5, 0.999999999782)370.366581635, 469.859086016, 50.8501186268
6given(-0.901848254673, 0.43205291964, 1.32140312607E-5), (-0.4320529198, -0.901848254588, -1.37571345627E-5), (5.97324087482E-6, -1.81160085831E-5, 0.999999999818)313.059110281, 497.129504531, 54.0284763304
7given(-0.791308588453, 0.611416975243, 1.49981553412E-5), (-0.611416975413, -0.791308588365, -1.2530237731E-5), (4.20696907852E-6, -1.90854115074E-5, 0.999999999809)251.30872495, 511.787900696, 57.207179333
8given(-0.645644595831, 0.763638039682, 1.49879939018E-5), (-0.763638039828, -0.645644595736, -1.11540728393E-5), (1.15924294616E-6, -1.86469691343E-5, 0.999999999825)187.858965213, 513.183532546, 60.3856540375
9given(-0.47131959423, 0.881962493441, 1.60507198449E-5), (-0.881962493587, -0.471319594149, -8.71720584968E-6), (-1.23229843936E-7, -1.82647228221E-5, 0.999999999833)125.524318249, 501.25400725, 63.5639364606
10given(0.299639965293, -0.954052352433, -4.0450715661E-6), (0.954052352375, 0.29963996532, -1.05670352704E-5), (1.12935699617E-5, -6.92903961491E-7, 0.999999999936)352.392938521, -54.0301867422, 19.0656885208
11given(0.0931007069435, -0.995656697036, -3.69170500831E-6), (0.995656696962, 0.0931007069827, -1.24446883713E-5), (1.27343376657E-5, -2.51706152967E-6, 0.999999999916)405.247249625, -18.8979569251, 22.2438073649
12given(-0.117570938595, -0.993064486523, -2.16718648373E-6), (0.993064486426, -0.117570938552, -1.44247683945E-5), (1.406992707E-5, -3.84808949162E-6, 0.999999999894)449.567568558, 26.5282989343, 25.4217823382
13given(-0.323024917245, -0.946390460031, -1.34334084975E-6), (0.946390459913, -0.323024917182, -1.62867365007E-5), (1.49796794826E-5, -6.53234667496E-6, 0.999999999866)483.386987484, 80.2326337496, 28.6002235547
14given(-0.514140677953, -0.85770587224, 5.63529447146E-7), (0.857705872099, -0.51414067788, -1.77837522413E-5), (1.55429621398E-5, -8.66000791798E-6, 0.999999999842)505.203682816, 139.830996777, 31.7785571432
15given(-0.682432008778, -0.730949077153, 2.12058730139E-6), (0.730949077006, -0.682432008694, -1.82868206177E-5), (1.48138913063E-5, -1.09294703976E-5, 0.999999999831)514.049556209, 202.676650443, 34.9571882193
16given(0.449474925146, 0.893292948333, 1.11295084971E-5), (-0.893292948389, 0.449474925172, 2.58304555685E-7), (-4.77169336083E-6, -1.00580128804E-5, 0.999999999938)-73.0166597268, 307.534479209, -111.226836784
17given(-0.0685734116455, 0.997646073015, 1.4563533884E-5), (-0.997646073117, -0.0685734115945, -3.97571363163E-6), (-2.96768388874E-6, -1.48018806375E-5, 0.999999999886)15.0993756464, 440.107099095, 69.9197645756
18given(0.141971379362, 0.989870762897, 1.42518784704E-5), (-0.989870762991, 0.141971379409, -2.30753459972E-6), (-4.30751988024E-6, -1.37799139455E-5, 0.999999999896)-28.090075245, 393.603825649, 73.0978948073
19given(0.346215117921, 0.938155153458, 1.27573263054E-5), (-0.938155153531, 0.346215117958, -6.96508085508E-7), (-5.07021188151E-6, -1.17272097898E-5, 0.999999999918)-60.5776186422, 339.083399975, 76.2756052636
20given(0.535088999503, 0.844795692753, 1.07680738067E-5), (-0.844795692806, 0.535088999528, 6.12799930191E-7), (-5.24418709852E-6, -9.42472487323E-6, 0.999999999942)-80.9211652431, 278.966912888, 79.4531389835
21given(0.700211641472, 0.713935331153, 8.79999972459E-6), (-0.713935331188, 0.700211641492, 1.1945848593E-6), (-5.30900591517E-6, -7.11909294304E-6, 0.999999999961)-88.2177851748, 215.92199868, 82.6306463872
22given(0.834251565131, 0.551384009597, 6.08233122967E-6), (-0.551384009613, 0.834251565142, 1.29482931503E-6), (-4.3602461686E-6, -4.43391356385E-6, 0.999999999981)-82.1434486214, 152.747814659, 85.807835497
23given(0.931259475653, 0.364356678256, 4.07207969939E-6), (-0.364356678266, 0.931259475655, 2.18858628959E-6), (-2.99473677513E-6, -3.52183115337E-6, 0.999999999989)-62.9679844619, 92.2480930124, 88.9854613282
24given(0.986929181171, 0.161154557328, 2.45686301259E-6), (-0.161154557331, 0.986929181172, 1.26783174355E-6), (-2.22043293787E-6, -1.64719481575E-6, 0.999999999996)-31.5422798541, 37.1087744307, 92.1628308205
25given(0.998789027691, -0.0491983553055, 4.54832309399E-7), (0.0491983553054, 0.998789027691, 2.46969806097E-7), (-4.66432028337E-7, -2.24293730938E-7, 1)10.7377915501, -10.2220997401, 95.3401406198
26given(0.966313441206, -0.257368089211, -1.03744922965E-6), (0.25736808921, 0.966313441206, -8.79905470147E-7), (1.22896072471E-6, 5.83258156907E-7, 0.999999999999)61.9961994259, -47.6443158128, 98.51760314
27given(0.890942823295, -0.454115498095, -3.05072552927E-6), (0.454115498086, 0.890942823299, -3.22804939076E-6), (4.18392927312E-6, 1.4906256947E-6, 0.99999999999)119.958443661, -73.4963648371, 101.694786186
28given(0.776026016432, -0.630700897263, -3.57562619549E-6), (0.630700897248, 0.776026016439, -4.38036083585E-6), (5.53747646227E-6, 1.14412332026E-6, 0.999999999984)182.048841649, -86.6311630024, 104.872560246
29given(0.626656985758, -0.77929520862, -4.73124090806E-6), (0.779295208592, 0.626656985776, -6.57767158797E-6), (8.0908131188E-6, 4.34910480274E-7, 0.999999999967)245.515666744, -86.464484339, 108.050227413
30given(0.449474670333, -0.893293076604, -4.5548532958E-6), (0.89329307656, 0.449474670356, -8.78056244734E-6), (9.89090682654E-6, -1.22178502526E-7, 0.999999999951)307.537084378, -73.0050476525, 111.227893347
31given(0.252340336393, -0.967638545435, -4.12832003375E-6), (0.967638545377, 0.252340336423, -1.06994546023E-5), (1.13949463545E-5, -1.29481761875E-6, 0.999999999934)365.36215598, -46.8499230428, 114.405811829
32given(0.0440032673207, -0.999031387123, -2.96793742202E-6), (0.999031387039, 0.044003267356, -1.31243167399E-5), (1.32422033016E-5, -2.38754982146E-6, 0.999999999909)416.424128584, -9.15949077565, 117.583632336
33given(-0.299639169431, 0.954052602265, 1.59654798921E-5), (-0.954052602397, -0.299639169361, -6.67311931998E-6), (-1.58262371915E-6, -1.72314355701E-5, 0.99999999985)73.6010490139, 480.040370467, 19.0720317437
34given(-0.0931010635958, 0.995656663588, 1.44644495854E-5), (-0.995656663687, -0.0931010635331, -4.95260509185E-6), (-3.584438622E-6, -1.48627184179E-5, 0.999999999883)20.7472489496, 444.908417205, 22.2498504046
35given(0.117572308754, 0.993064324207, 1.42093788928E-5), (-0.993064324301, 0.117572308797, -2.2308323182E-6), (-3.8859894715E-6, -1.38485431428E-5, 0.999999999897)-23.5735027836, 399.481399453, 25.4278266068
36given(0.323025602953, 0.946390225902, 1.24194790971E-5), (-0.94639022597, 0.323025602989, -9.97035035008E-7), (-4.95539393618E-6, -1.14316057858E-5, 0.999999999922)-57.392581051, 345.777361615, 28.6054682239
37given(0.514140438584, 0.857706015661, 1.05207432381E-5), (-0.857706015712, 0.514140438608, 5.3572194545E-7), (-4.9496476076E-6, -9.2991410811E-6, 0.999999999945)-79.2092045333, 286.179271254, 31.7830342572
38given(0.682432729432, 0.730948404284, 8.64815132103E-6), (-0.730948404316, 0.682432729452, 8.34747277097E-7), (-5.29162432055E-6, -6.89101127108E-6, 0.999999999962)-88.0548649914, 223.333172584, 34.9605477458
39given(0.820431288252, 0.571745136598, 5.86623827297E-6), (-0.571745136615, 0.820431288259, 1.70083890431E-6), (-3.84039905185E-6, -4.74941465617E-6, 0.999999999981)-83.536953402, 160.028788213, 38.1378271341
40given(0.922012087874, 0.387161090226, 5.39043843148E-6), (-0.387161090241, 0.922012087881, 2.20407636996E-6), (-4.11671678247E-6, -4.11915307572E-6, 0.999999999983)-65.856222151, 99.0755237839, 41.3158026981
41given(0.982664478864, 0.185392885445, 2.29427872432E-6), (-0.185392885447, 0.982664478866, 7.25329779475E-7), (-2.12003522629E-6, -1.13809876247E-6, 0.999999999997)-35.7972095495, 43.180336067, 44.4926524194
42given(0.99969720456, -0.0246068932247, 5.83831719131E-7), (0.0246068932244, 0.99969720456, 4.81450497333E-7), (-5.95501938529E-7, -4.66938431544E-7, 1)5.30601391475, -5.17721732236, 47.6702346609
43given(0.972353522686, -0.233513654674, -1.05958873055E-6), (0.233513654674, 0.972353522686, -5.96099517077E-7), (1.16949221153E-6, 3.32191028379E-7, 0.999999999999)55.6284239011, -43.8494607103, 50.8476944153
44given(0.901847956399, -0.432053542439, -2.15781599213E-6), (0.432053542433, 0.9018479564, -2.73487418796E-6), (3.12763402382E-6, 1.53414865411E-6, 0.999999999994)112.935860724, -71.1198582327, 54.0249484271
45given(0.791308787032, -0.611416718412, -3.48219912164E-6), (0.611416718395, 0.791308787038, -4.77269730422E-6), (5.67360168689E-6, 1.64760255492E-6, 0.999999999983)174.685837069, -85.7781677221, 57.2023990501
46given(0.645645487231, -0.763637286155, -3.65301434285E-6), (0.763637286131, 0.64564548724, -6.14784671408E-6), (7.05327720572E-6, 1.17975152816E-6, 0.999999999974)238.135413264, -87.174012136, 60.3801719191
47given(0.4713186848, -0.881962979573, -4.57457581954E-6), (0.881962979528, 0.471318684823, -8.86038652057E-6), (9.97061595473E-6, 1.41459201818E-7, 0.99999999995)300.470724261, -75.2440863742, 63.5578308042
48given(0.276074814576, -0.96113614891, -3.99257880447E-6), (0.961136148842, 0.276074814604, -1.13950252537E-5), (1.20544211423E-5, -6.91532332085E-7, 0.999999999927)358.921434302, -50.5199965008, 66.7355495303
49given(0.0685737286503, -0.997646051327, -3.19893642425E-6), (0.99764605125, 0.0685737286855, -1.26536021082E-5), (1.28431791768E-5, -2.32370161443E-6, 0.999999999915)410.895421023, -14.0978066543, 69.9137090418
50given(-0.141971578408, -0.989870734448, -2.64788334725E-6), (0.989870734347, -0.141971578353, -1.49355364692E-5), (1.44083262761E-5, -4.7414839203E-6, 0.999999999885)454.084927573, 32.4057170995, 73.0919790929
51given(-0.346214644634, -0.938155328205, -1.11501388699E-6), (0.938155328089, -0.346214644572, -1.61756631375E-5), (1.47892504231E-5, -6.64630768383E-6, 0.999999999869)486.572490824, 86.9257729225, 76.2702530648
52given(-0.535089230847, -0.84479554629, 1.00855582484E-6), (0.844795546149, -0.535089230779, -1.76405538668E-5), (1.54423287012E-5, -8.5872469314E-6, 0.999999999844)506.91604308, 147.042503394, 79.4485316177
53given(-0.700211259242, -0.713935706085, 2.73920183136E-6), (0.713935705929, -0.70021125916, -1.85617720741E-5), (1.51699318153E-5, -1.10415478046E-5, 0.999999999824)514.212787404, 210.087154274, 82.6271460852
54given(-0.834251015318, -0.551384841486, 4.47702586082E-6), (0.551384841325, -0.834251015226, -1.87310379005E-5), (1.40629737332E-5, -1.31578231926E-5, 0.999999999815)508.138818788, 273.261182846, 85.8058133605
55given(-0.931259480247, -0.364356666469, 7.12044796281E-6), (0.364356666293, -0.931259480159, -1.85189156961E-5), (1.3378475058E-5, -1.46515331237E-5, 0.999999999803)488.963093841, 333.760938869, 88.9842667597
56given(-0.986928767923, -0.161157087901, 8.09791410477E-6), (0.16115708774, -0.986928767824, -1.76692835639E-5), (1.08395946738E-5, -1.61332880039E-5, 0.999999999811)457.538249069, 388.899680965, 92.1630451376
57given(-0.998789043674, 0.0491980295877, 1.1007753714E-5), (-0.0491980297751, -0.998789043573, -1.74618317709E-5), (1.01353360878E-5, -1.79822460503E-5, 0.999999999787)415.257426738, 436.231123576, 95.3417040523
58given(-0.966313136547, 0.257369232771, 1.2627558958E-5), (-0.257369232952, -0.966313136453, -1.57556891652E-5), (8.1471464702E-6, -1.84748745788E-5, 0.999999999796)363.998525858, 473.653299088, 98.5201884767
59given(-0.890942822798, 0.454115498875, 1.36886950828E-5), (-0.454115499043, -0.890942822709, -1.38731365122E-5), (5.89583832805E-6, -1.8576420004E-5, 0.99999999981)306.036904956, 499.505310859, 101.698639616
60given(-0.776023965098, 0.630703421091, 1.47424865226E-5), (-0.630703421252, -0.776023965012, -1.21788163008E-5), (3.75930173969E-6, -1.87491900035E-5, 0.999999999817)243.94536189, 512.639714843, 104.877155463
61given(-0.626658337305, 0.779294121653, 1.55993695779E-5), (-0.779294121806, -0.626658337236, -9.59675802111E-6), (2.29677788882E-6, -1.81703854409E-5, 0.999999999832)180.480288086, 512.473380261, 108.055352967
62given(-0.449475396729, 0.893292710972, 1.61047122297E-5), (-0.893292711118, -0.449475396655, -8.15293398034E-6), (-4.4284580213E-8, -1.80507652848E-5, 0.999999999837)118.458412711, 499.013989757, 111.233880729
63given(-0.252340462211, 0.967638512501, 1.59978177414E-5), (-0.967638512632, -0.252340462147, -5.90021886023E-6), (-1.67238227912E-6, -1.6968968519E-5, 0.999999999855)60.6331127157, 472.858591936, 114.412020643
64given(-0.0440024524846, 0.99903142291, 1.46266131318E-5), (-0.99903142301, -0.0440024524234, -4.47819301674E-6), (-3.83024869313E-6, -1.48094976063E-5, 0.999999999883)9.57105064061, 435.168027723, 117.589929713
65given(-0.369196132815, 0.929351502533, 1.59120588063E-5), (-0.929351502669, -0.369196132735, -7.81006678558E-6), (-1.38362672693E-6, -1.76713422165E-5, 0.999999999843)93.6771353244, 489.596634366, -123.937949259
66given(-0.166287637967, 0.986077289683, 1.51721459366E-5), (-0.986077289794, -0.166287637894, -5.98471473645E-6), (-3.37845097726E-6, -1.5956092623E-5, 0.999999999867)38.3753409106, 458.458460099, -120.75993598
67given(0.0440025293848, 0.999031419523, 1.45953537052E-5), (-0.999031419621, 0.0440025294424, -3.65286944691E-6), (-4.29156383001E-6, -1.44204814368E-5, 0.999999999887)-9.17521245188, 416.42457951, -117.582005662
68given(0.252339309316, 0.967638813205, 1.23977472597E-5), (-0.96763881327, 0.25233930936, -2.08738125411E-6), (-5.14827010057E-6, -1.14698131017E-5, 0.999999999921)-46.8633742597, 365.36115296, -114.404570681
69given(0.626657756813, 0.779294588536, 1.01751877441E-5), (-0.779294588581, 0.62665775684, 7.73845273623E-7), (-5.77330689307E-6, -8.4144048901E-6, 0.999999999948)-86.474076519, 245.512364967, -108.048965671
70given(0.0931007023906, 0.995656697368, 1.41374459513E-5), (-0.99565669746, 0.0931007024398, -2.86265701813E-6), (-4.16642978114E-6, -1.38095273673E-5, 0.999999999896)-18.9135514868, 405.246660379, -22.242162801
71given(0.299639667376, 0.954052445921, 1.29410576291E-5), (-0.954052445996, 0.299639667415, -1.13503104605E-6), (-4.96053334965E-6, -1.20063473597E-5, 0.999999999916)-54.0439275411, 352.391169721, -19.0643823724
72given(0.492879216437, 0.870097740412, 1.15391974174E-5), (-0.870097740472, 0.492879216467, 2.79749330086E-7), (-5.44402132176E-6, -1.01781122303E-5, 0.999999999933)-77.3205864141, 293.347874253, -15.8866439707
73given(0.664238766216, 0.747520475553, 9.21128042527E-6), (-0.747520475584, 0.664238766245, -4.61703551612E-8), (-6.15300283106E-6, -6.85495258449E-6, 0.999999999958)-87.7100532661, 230.738999526, -12.709353978
74given(0.806113891435, 0.591760419422, 6.40933410645E-6), (-0.591760419443, 0.806113891443, 1.76653112373E-6), (-4.12129005941E-6, -5.21681551767E-6, 0.999999999978)-84.7511394527, 167.342073834, -9.53200602805
75given(0.776024284736, 0.630703027944, 6.51862191707E-6), (-0.630703027962, 0.776024284747, 1.1502612832E-6), (-4.33313563649E-6, -5.00394527079E-6, 0.999999999978)-86.6375219842, 182.046887578, -104.872077223
76given(0.890943574081, 0.454114025082, 5.27889673302E-6), (-0.454114025094, 0.890943574089, 1.49399078575E-6), (-4.02475695341E-6, -3.72828253379E-6, 0.999999999985)-73.5006268338, 119.9554561, -101.694363015
77given(0.966313310405, 0.257368580305, 2.53873696529E-6), (-0.25736858031, 0.966313310406, 1.43833065226E-6), (-2.0830342032E-6, -2.04326918258E-6, 0.999999999996)-47.646254348, 61.9949910463, -98.5170951585
78given(0.998789057834, 0.0491977433274, 1.58151729833E-6), (-0.0491977433276, 0.998789057835, 1.21781871012E-7), (-1.57361077912E-6, -1.99441482321E-7, 0.999999999999)-10.2221537681, 10.7377834733, -95.339701888
79given(0.986928979412, -0.161155792936, -5.01888708179E-7), (0.161155792936, 0.986928979412, 3.32431514746E-8), (4.89971184106E-7, -1.1369090229E-7, 1)37.110441826, -31.5409590131, -92.1620463605
80given(0.931259696659, -0.3643561134, -2.64032995493E-6), (0.364356113395, 0.931259696662, -1.99682501323E-6), (3.18638827388E-6, 8.97542295642E-7, 0.999999999995)92.2507138583, -62.9641707259, -88.984838713
81given(0.834251407393, -0.551384248281, -3.26848137116E-6), (0.551384248265, 0.834251407396, -4.72465581928E-6), (5.33183598124E-6, 2.13936162287E-6, 0.999999999983)152.751199374, -82.1370621178, -85.80765086
82given(0.70021140767, -0.713935560505, -3.93901967715E-6), (0.713935560478, 0.700211407679, -6.38991367538E-6), (7.32013311442E-6, 1.66208422859E-6, 0.999999999972)215.92562409, -88.208878357, -82.6299521136
83given(0.535090896633, -0.844794491176, -4.38033720681E-6), (0.844794491141, 0.535090896651, -7.74679264462E-6), (8.88832631408E-6, 4.44753480588E-7, 0.99999999996)278.969523607, -80.9102927652, -79.45201561
84given(0.346216221183, -0.938154746388, -4.48573984528E-6), (0.938154746334, 0.346216221211, -1.00394400803E-5), (1.0971584261E-5, -7.32501119277E-7, 0.99999999994)339.085608712, -60.5643907457, -76.2742048482
85given(0.141972323771, -0.989870627541, -3.9035919776E-6), (0.989870627471, 0.141972323808, -1.19405691605E-5), (1.23738207123E-5, -2.1688206894E-6, 0.999999999921)393.604730269, -28.0745900185, -73.0961882133
86given(-0.0685714238658, -0.997646209747, -2.46452693377E-6), (0.997646209663, -0.0685714238269, -1.3416780581E-5), (1.32162041727E-5, -3.37873370223E-6, 0.999999999907)440.106213144, 15.115939876, -69.9181861123
87given(-0.276072698694, -0.961136756675, -1.92691674591E-6), (0.961136756571, -0.276072698634, -1.53960229173E-5), (1.42657144263E-5, -6.10245210728E-6, 0.99999999988)476.526865334, 67.0913348327, -66.7397408847
88given(-0.471319388047, -0.881962603771, 1.27174948637E-7), (0.881962603639, -0.471319387978, -1.72900015818E-5), (1.53090748333E-5, -8.03694941606E-6, 0.999999999851)501.249244467, 125.543925736, -63.5615560197
89given(-0.645643264054, -0.763639165822, 2.0700951958E-6), (0.763639165682, -0.645643263983, -1.76296746107E-5), (1.47992530324E-5, -9.80167489164E-6, 0.999999999842)513.176313118, 187.878247616, -60.3831192297
90given(-0.791309328713, -0.61141601735, 4.40113193671E-6), (0.611416017199, -0.791309328645, -1.76566959503E-5), (1.42782434756E-5, -1.12809856598E-5, 0.999999999834)511.778175238, 251.328927565, -57.2047500829
91given(-0.90184783396, -0.432053797974, 6.19858507519E-6), (0.432053797805, -0.901847833872, -1.85170607219E-5), (1.35905469354E-5, -1.40214488806E-5, 0.999999999809)497.117540474, 313.077975759, -54.0259234939
92given(-0.972353511024, -0.233513703102, 7.96402579846E-6), (0.233513702932, -0.972353510929, -1.80688614953E-5), (1.19631752049E-5, -1.57096117608E-5, 0.999999999805)469.845132797, 370.384533816, -50.8472443656
93given(-0.999697230657, -0.0246058308593, 1.02026833914E-5), (0.0246058306802, -0.999697230563, -1.73265415705E-5), (1.0625928282E-5, -1.70702501249E-5, 0.999999999798)431.170594644, 420.70559681, -47.6686526464
94given(-0.982664694827, 0.185391740363, 1.21121109119E-5), (-0.185391740545, -0.982664694732, -1.6250161526E-5), (8.88949804531E-6, -1.82139453404E-5, 0.999999999795)382.812224713, 461.806544725, -44.490022092
95given(-0.9220114687, 0.387162564553, 1.39115005334E-5), (-0.387162564737, -0.922011468612, -1.46394191991E-5), (7.15872795666E-6, -1.88837246225E-5, 0.999999999796)326.914821571, 491.863530458, -41.3114827492
96given(-0.820431948405, 0.571744189157, 1.41942656383E-5), (-0.571744189318, -0.820431948312, -1.30120027033E-5), (4.20589207758E-6, -1.87909516309E-5, 0.999999999815)265.962080711, 509.542116933, -38.1329510191
97given(-0.682432040467, 0.730949047414, 1.51155903918E-5), (-0.730949047566, -0.682432040387, -1.07500508423E-5), (2.4576237699E-6, -1.83849055317E-5, 0.999999999828)202.656934263, 514.057364489, -34.9546694718
98given(-0.514138967634, 0.857706897307, 1.64778290138E-5), (-0.857706897466, -0.514138967545, -9.58946187731E-6), (2.46946402926E-7, -1.90634636302E-5, 0.999999999818)139.81065684, 505.209133608, -31.7759157867
99given(-0.323024895615, 0.946390467286, 1.5608684507E-5), (-0.946390467414, -0.323024895553, -6.35684200865E-6), (-9.74060996428E-7, -1.68253284526E-5, 0.999999999858)80.2140181491, 483.389975593, -28.598208692
100given(-0.117570802941, 0.993064502469, 1.52258190274E-5), (-0.993064502582, -0.117570802884, -4.56102274002E-6), (-2.73927801045E-6, -1.56564635046E-5, 0.999999999874)26.5110619617, 449.568574768, -25.4200609505
101given(0.276072100367, 0.961136928454, 1.2687510573E-5), (-0.961136928523, 0.276072100411, -1.79560510573E-6), (-5.2284900689E-6, -1.16987184697E-5, 0.999999999918)-50.5333763003, 358.9207316, -66.7344613391
102given(0.471320289515, 0.881962121959, 1.10354372914E-5), (-0.881962122011, 0.471320289545, -1.93958892646E-7), (-5.37228989598E-6, -9.64142092942E-6, 0.999999999939)-75.2558404479, 300.46755365, -63.5568492111
103given(0.645643475929, 0.76363898663, 9.4254524538E-6), (-0.763638986671, 0.645643475948, 1.36010998384E-6), (-5.04684887489E-6, -8.07578909835E-6, 0.999999999955)-87.1829343094, 238.133205284, -60.3791656817
104given(0.791308087955, 0.611417623145, 6.63704613829E-6), (-0.611417623164, 0.791308087968, 1.08307276745E-6), (-4.58973851228E-6, -4.91505121543E-6, 0.999999999977)-85.7849867658, 174.683259153, -57.2020500165
105given(0.901847267738, 0.432054979889, 5.16463924481E-6), (-0.4320549799, 0.901847267747, 1.11818285884E-6), (-4.17459931924E-6, -3.23983826118E-6, 0.999999999986)-71.1243616227, 112.934028557, -54.0244855629
106given(0.912205614632, 0.409732738022, 5.14327993339E-6), (-0.409732738034, 0.91220561464, 1.53052089438E-6), (-4.06462431625E-6, -3.50351992275E-6, 0.999999999986)-68.5756755811, 105.972571487, -6.3544074801
107given(0.977805448642, 0.209514926916, 2.12510936284E-6), (-0.209514926919, 0.977805448643, 1.39451007078E-6), (-1.78577283839E-6, -1.80880167825E-6, 0.999999999997)-39.9003617768, 49.3536032363, -3.1771957381
108given(0.982664516571, -0.185392685595, -8.44201904576E-7), (0.185392685595, 0.982664516571, -4.23890949662E-7), (9.08153538006E-7, 2.60033736855E-7, 1)43.1816969187, -35.7952787169, -44.4922311965
109given(0.977805002906, -0.209517007166, -6.86165700443E-7), (0.209517007165, 0.977805002906, -6.66514705628E-7), (8.10582421071E-7, 5.07958029697E-7, 1)49.3555969033, -39.8989649631, 3.17770068626
110given(0.912205760527, -0.409732413236, -2.15993135984E-6), (0.409732413232, 0.912205760529, -2.05766766056E-6), (2.813394965E-6, 9.92022404726E-7, 0.999999999996)105.974989924, -68.571377822, 6.35517313476
111given(0.80611505063, -0.591758840352, -3.65765949179E-6), (0.59175884034, 0.806115050638, -3.79053053813E-6), (5.191574322E-6, 8.91151377435E-7, 0.999999999986)167.344649301, -84.7448448879, 9.53272275466
112given(0.664238771956, -0.747520470496, -4.36355018823E-6), (0.747520470467, 0.66423877197, -6.72075312202E-6), (7.92233975432E-6, 1.20234171078E-6, 0.999999999968)230.742067415, -87.7008003914, 12.7100508022
113given(0.49288014906, -0.870097212179, -4.59626172134E-6), (0.870097212141, 0.492880149082, -8.13917913461E-6), (9.34728323688E-6, 1.24453150883E-8, 0.999999999956)293.350477421, -77.3093975127, 15.8879936541
114given(0.369195382807, -0.929351800608, -4.62994220036E-6), (0.92935180056, 0.369195382835, -9.43668296283E-6), (1.04793515864E-5, -8.18865341509E-7, 0.999999999945)332.315780457, -63.5836326495, -123.944075198
115given(0.166288156434, -0.986077202361, -3.61318185807E-6), (0.986077202286, 0.166288156467, -1.23087793829E-5), (1.27382360885E-5, -1.51607202642E-6, 0.999999999918)387.617228378, -32.4456539649, -120.766423512
116given(-0.0440032310701, -0.999031388719, -3.18474157511E-6), (0.999031388629, -0.0440032310219, -1.3888392748E-5), (1.37348013748E-5, -3.79279095349E-6, 0.999999999898)435.168059792, 9.58842495609, -117.588098388
117given(-0.252337463582, -0.967639294608, -1.95192571621E-6), (0.967639294501, -0.252337463522, -1.55097864124E-5), (1.45153347994E-5, -5.80246018693E-6, 0.999999999878)472.856207927, 60.6509155874, -114.409841459
118given(-0.449475578247, -0.893292619783, -1.09674876094E-7), (0.893292619662, -0.449475578184, -1.65270065272E-5), (1.47141567795E-5, -7.52645757289E-6, 0.999999999863)499.009539157, 118.478150995, -111.23158711
119given(-0.626658649664, -0.779293870629, 7.85764236982E-7), (0.779293870505, -0.626658649582, -1.72432427575E-5), (1.39299593463E-5, -1.01932859687E-5, 0.999999999851)512.466939468, 180.50039882, -108.052836601
120given(-0.776024134903, -0.63070321232, 4.2077803111E-6), (0.630703212166, -0.776024134833, -1.79105363708E-5), (1.45615718989E-5, -1.12451479345E-5, 0.999999999831)512.630591969, 243.965439779, -104.874787812
121given(-0.890943266959, -0.454114627619, 6.63094999546E-6), (0.454114627439, -0.890943266879, -1.87142382858E-5), (1.44062096018E-5, -1.36621132102E-5, 0.999999999803)499.49360601, 306.056805383, -101.696147003
122given(-0.966313216684, -0.257368932058, 8.53271706985E-6), (0.257368931881, -0.966313216606, -1.77402852567E-5), (1.28110755491E-5, -1.4946615833E-5, 0.999999999806)473.639388577, 364.017112078, -98.517603195
123given(-0.998789045489, -0.0491979929204, 1.02160587137E-5), (0.0491979927377, -0.998789045398, -1.74208118399E-5), (1.1060756508E-5, -1.68971064468E-5, 0.999999999796)436.21515572, 415.274521638, -95.3387717174
124given(-0.986929068661, 0.16115524594, 1.17082599275E-5), (-0.161155246114, -0.986929068575, -1.58414629285E-5), (9.00228721063E-6, -1.75212477644E-5, 0.999999999806)388.882809108, 457.552890174, -92.1602558364
125given(-0.931259698834, 0.364356107596, 1.36200218708E-5), (-0.364356107781, -0.931259698737, -1.51822958806E-5), (7.15201523281E-6, -1.91011984461E-5, 0.999999999792)333.742438714, 488.976378085, -88.9814273499
126given(-0.834251460909, 0.551384167145, 1.38825751796E-5), (-0.551384167301, -0.834251460824, -1.27728846485E-5), (4.53879225965E-6, -1.83104298334E-5, 0.999999999822)273.242118922, 508.149282132, -85.8031655648
127given(-0.700211953426, 0.713935025093, 1.49722297436E-5), (-0.713935025245, -0.700211953349, -1.07513221134E-5), (2.80798881196E-6, -1.82174034789E-5, 0.99999999983)210.067833458, 514.221045681, -82.6247835076
128given(-0.535089223653, 0.844795550706, 1.54012218577E-5), (-0.844795550846, -0.535089223569, -9.51290698798E-6), (2.04566348129E-7, -1.81011377179E-5, 0.999999999836)147.02316739, 506.921999066, -79.4462330267
129given(-0.346214767717, 0.938155282652, 1.57362768473E-5), (-0.938155282783, -0.346214767643, -7.2855056717E-6), (-1.38680420043E-6, -1.72854209095E-5, 0.99999999985)86.9072329866, 486.576036838, -76.2680388398
130given(-0.141972658456, 0.989870579429, 1.52248539297E-5), (-0.989870579543, -0.141972658403, -4.52743988142E-6), (-2.32006655256E-6, -1.57134076588E-5, 0.999999999874)32.388595982, 454.086514078, -73.0901428742
131given(0.0685714800813, 0.997646205791, 1.37892083327E-5), (-0.997646205876, 0.0685714801358, -3.52069066487E-6), (-4.45795010884E-6, -1.35153324053E-5, 0.999999999899)-14.1129103143, 410.895871188, -69.9122287853
132given(0.972353493151, 0.233513777645, 2.59125277683E-6), (-0.233513777646, 0.972353493154, 4.73129572394E-7), (-2.40913141543E-6, -1.06514241718E-6, 0.999999999997)-43.8514912527, 55.6271404547, -50.8473366132
133given(-0.276073205438, 0.961136611003, 1.51561699485E-5), (-0.961136611121, -0.276073205379, -5.91799649981E-6), (-1.5037906808E-6, -1.62009500854E-5, 0.999999999868)67.0730350106, 476.52915028, 66.741728035
134given(0.999697201084, 0.0246070344541, 8.83453038901E-7), (-0.0246070344545, 0.999697201084, 3.43107538879E-7), (-8.74742671247E-7, -3.64742805655E-7, 1)-5.17736685161, 5.30606659798, -47.6697562301
135given(0.922012598804, -0.38715987349, -2.20760776964E-6), (0.387159873485, 0.922012598806, -2.39656591528E-6), (2.9632963334E-6, 1.35496682295E-6, 0.999999999995)99.0777217468, -65.8521069561, -41.3149526759
136given(0.820430981892, -0.571745576232, -3.37754742907E-6), (0.571745576218, 0.820430981897, -4.38933235811E-6), (5.28062591199E-6, 1.67004645538E-6, 0.999999999985)160.032249132, -83.530651155, -38.1375175512
137given(0.682433366005, -0.730947810005, -3.50498187308E-6), (0.730947809982, 0.682433366012, -6.09290508178E-6), (6.84551220356E-6, 1.59604289955E-6, 0.999999999975)223.336115274, -88.0463634107, -34.9598793582
138given(0.51414088472, -0.857705748278, -5.45215652254E-6), (0.857705748236, 0.514140884749, -8.53713483162E-6), (1.01255261972E-5, -2.87055934355E-7, 0.999999999949)286.18246301, -79.1981430146, -31.7820085139
139given(0.323025225332, -0.946390354863, -4.61389712975E-6), (0.946390354811, 0.323025225363, -1.00203165284E-5), (1.09735360753E-5, -1.1297327372E-6, 0.999999999939)345.779722692, -57.3790442976, -28.6041002506
140given(0.117572788159, -0.993064267544, -3.29438087681E-6), (0.993064267466, 0.117572788192, -1.25809184136E-5), (1.28809900745E-5, -1.7923582767E-6, 0.999999999915)399.482222357, -23.55801908, -25.4264188137
141given(-0.0931003865301, -0.995656727, -2.64335568818E-6), (0.995656726915, -0.0931003864861, -1.35826760668E-5), (1.32775853604E-5, -3.8964272645E-6, 0.999999999904)444.907287358, 20.7641873104, -22.2480610512
142given(-0.299640376203, -0.954052223385, -1.70627568438E-6), (0.954052223279, -0.299640376142, -1.5522719482E-5), (1.4298215947E-5, -6.27910961551E-6, 0.999999999878)480.037846386, 73.6199998791, -19.0697224108
143given(-0.492880204686, -0.870097180681, 7.20161375557E-8), (0.870097180547, -0.492880204612, -1.75102042335E-5), (1.52710746653E-5, -8.56777200846E-6, 0.999999999847)503.314419756, 132.663492484, -15.8914065192
144given(-0.664238832085, -0.747520417077, 2.08334239664E-6), (0.747520416932, -0.664238832006, -1.80482541604E-5), (1.48752753977E-5, -1.04310102877E-5, 0.999999999835)513.703999085, 195.272122336, -12.7129582921
145given(-0.806113898424, -0.59176040992, 4.3639322566E-6), (0.591760409764, -0.806113898346, -1.81444126054E-5), (1.42549714846E-5, -1.20440608396E-5, 0.999999999826)510.745216799, 258.66862579, -9.53452905813
146given(-0.912206115743, -0.40973162236, 6.30733544873E-6), (0.409731622196, -0.912206115658, -1.81251582512E-5), (1.31800404656E-5, -1.39495654204E-5, 0.999999999816)494.569392561, 320.038432181, -6.35589456899
147given(-0.977805044058, -0.209516814968, 7.7875545327E-6), (0.209516814798, -0.977805043951, -1.84597234567E-5), (1.1482332566E-5, -1.64183870871E-5, 0.999999999799)465.895206298, 376.65671157, -3.17695304619
148given(-0.999999999946, -1.0319316633E-7, 1.03949152812E-5), (1.0300916656E-7, -0.999999999843, -1.77009388644E-5), (1.03949171062E-5, -1.77009377927E-5, 0.999999999789)425.994302365, 426.010784133, 0.00157813664975
149given(-0.977805599583, 0.209514222148, 1.18735480575E-5), (-0.209514222321, -0.977805599489, -1.59323244888E-5), (8.2719732041E-6, -1.8066393287E-5, 0.999999999803)376.639513745, 465.909248043, 3.1800331994
150given(-0.912205907893, 0.409732084935, 1.34391130534E-5), (-0.409732085105, -0.912205907803, -1.42732873077E-5), (6.41101455546E-6, -1.85266128205E-5, 0.999999999808)320.019282166, 494.581774242, 6.35852564639
151given(-0.80611419188, 0.591760009989, 1.50528024513E-5), (-0.591760010166, -0.806114191781, -1.33254266566E-5), (4.24882307069E-6, -1.96494620723E-5, 0.999999999798)258.649041465, 510.755273782, 9.53732958663
152given(-0.66424052258, 0.747518914764, 1.5256671621E-5), (-0.747518914917, -0.664240522493, -1.09002250535E-5), (1.98597512636E-6, -1.86450218011E-5, 0.999999999824)195.253010972, 513.711679926, 12.7155065718
153given(-0.492879222006, 0.870097737194, 1.56366241888E-5), (-0.870097737335, -0.492879221932, -8.50701724302E-6), (3.05030710389E-7, -1.77983233665E-5, 0.999999999842)132.643516891, 503.319497262, 15.8937030549

-
Components

#1: Protein ...
CanX


Mass: 18745.406 Da / Num. of mol.: 154
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pyrodictium abyssi DSM 6158 (archaea) / Production host: Pyrodictium abyssi DSM 6158 (archaea)
#2: Chemical...
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 462 / Source method: obtained synthetically / Formula: Ca
#3: Sugar...
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 154 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestN
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

-
Sample preparation

ComponentName: Ex vivo CanX fibers from Pyrodictium abyssi / Type: COMPLEX / Entity ID: #1 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Pyrodictium abyssi DSM 6158 (archaea)
Buffer solutionpH: 5.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid type: Quantifoil R2/1
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

MicroscopyModel: JEOL CRYO ARM 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1600 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

-
Processing

EM softwareName: PHENIX / Version: 1.21_5207 / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: 12.094 ° / Axial rise/subunit: 3.178 Å / Axial symmetry: C2
3D reconstructionResolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 310172 / Symmetry type: HELICAL
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 24.64 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0028181874
ELECTRON MICROSCOPYf_angle_d0.6768247786
ELECTRON MICROSCOPYf_chiral_restr0.053930646
ELECTRON MICROSCOPYf_plane_restr0.004131108
ELECTRON MICROSCOPYf_dihedral_angle_d6.525227566
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2ZCEEELECTRON MICROSCOPYNCS constraints4.26866870113E-13
ens_1d_3ZCEEELECTRON MICROSCOPYNCS constraints9.69954789303E-13
ens_1d_4ZCEEELECTRON MICROSCOPYNCS constraints1.02115232796E-12
ens_1d_5ZCEEELECTRON MICROSCOPYNCS constraints1.05391011639E-12
ens_1d_6ZCEEELECTRON MICROSCOPYNCS constraints4.62608789419E-13
ens_1d_7ZCEEELECTRON MICROSCOPYNCS constraints9.01796944821E-13
ens_1d_8ZCEEELECTRON MICROSCOPYNCS constraints1.14399448697E-12
ens_1d_9ZCEEELECTRON MICROSCOPYNCS constraints2.76457993983E-12
ens_1d_10ZCEEELECTRON MICROSCOPYNCS constraints2.83131203695E-11
ens_1d_11ZCEEELECTRON MICROSCOPYNCS constraints3.3023404155E-12
ens_1d_12ZCEEELECTRON MICROSCOPYNCS constraints7.03841316223E-13
ens_1d_13ZCEEELECTRON MICROSCOPYNCS constraints7.86783955399E-13
ens_1d_14ZCEEELECTRON MICROSCOPYNCS constraints8.28388387065E-13
ens_1d_15ZCEEELECTRON MICROSCOPYNCS constraints6.83618506047E-13
ens_1d_16ZCEEELECTRON MICROSCOPYNCS constraints9.75191274223E-13
ens_1d_17ZCEEELECTRON MICROSCOPYNCS constraints1.4386374234E-12
ens_1d_18ZCEEELECTRON MICROSCOPYNCS constraints7.67129824941E-13
ens_1d_19ZCEEELECTRON MICROSCOPYNCS constraints7.50195693014E-13
ens_1d_20ZCEEELECTRON MICROSCOPYNCS constraints1.18463266542E-11
ens_1d_21ZCEEELECTRON MICROSCOPYNCS constraints7.26843806023E-13
ens_1d_22ZCEEELECTRON MICROSCOPYNCS constraints1.26305219527E-11
ens_1d_23ZCEEELECTRON MICROSCOPYNCS constraints1.40841071918E-11
ens_1d_24ZCEEELECTRON MICROSCOPYNCS constraints6.03326448239E-11
ens_1d_25ZCEEELECTRON MICROSCOPYNCS constraints7.18471276612E-11
ens_1d_26ZCEEELECTRON MICROSCOPYNCS constraints9.99661427537E-13
ens_1d_27ZCEEELECTRON MICROSCOPYNCS constraints9.85742031463E-13
ens_1d_28ZCEEELECTRON MICROSCOPYNCS constraints3.21379277915E-13
ens_1d_29ZCEEELECTRON MICROSCOPYNCS constraints1.28987612925E-11
ens_1d_30ZCEEELECTRON MICROSCOPYNCS constraints3.43111736509E-11
ens_1d_31ZCEEELECTRON MICROSCOPYNCS constraints9.73720034307E-11
ens_1d_32ZCEEELECTRON MICROSCOPYNCS constraints1.58858013947E-12
ens_1d_33ZCEEELECTRON MICROSCOPYNCS constraints8.47169766946E-13
ens_1d_34ZCEEELECTRON MICROSCOPYNCS constraints7.48894459291E-12
ens_1d_35ZCEEELECTRON MICROSCOPYNCS constraints7.61587013871E-13
ens_1d_36ZCEEELECTRON MICROSCOPYNCS constraints5.41655707047E-13
ens_1d_37ZCEEELECTRON MICROSCOPYNCS constraints1.63811802756E-11
ens_1d_38ZCEEELECTRON MICROSCOPYNCS constraints1.18980697374E-12
ens_1d_39ZCEEELECTRON MICROSCOPYNCS constraints6.55551075039E-12
ens_1d_40ZCEEELECTRON MICROSCOPYNCS constraints1.4498183674E-11
ens_1d_41ZCEEELECTRON MICROSCOPYNCS constraints6.94401258027E-11
ens_1d_42ZCEEELECTRON MICROSCOPYNCS constraints6.65822405747E-11
ens_1d_43ZCEEELECTRON MICROSCOPYNCS constraints6.9490508966E-13
ens_1d_44ZCEEELECTRON MICROSCOPYNCS constraints1.38651345793E-12
ens_1d_45ZCEEELECTRON MICROSCOPYNCS constraints7.79200853224E-13
ens_1d_46ZCEEELECTRON MICROSCOPYNCS constraints9.68971302489E-12
ens_1d_47ZCEEELECTRON MICROSCOPYNCS constraints3.52406942284E-11
ens_1d_48ZCEEELECTRON MICROSCOPYNCS constraints9.86063639647E-11
ens_1d_49ZCEEELECTRON MICROSCOPYNCS constraints2.29242182027E-12
ens_1d_50ZCEEELECTRON MICROSCOPYNCS constraints5.46309472419E-13
ens_1d_51ZCEEELECTRON MICROSCOPYNCS constraints6.28971892031E-13
ens_1d_52ZCEEELECTRON MICROSCOPYNCS constraints5.59360215201E-13
ens_1d_53ZCEEELECTRON MICROSCOPYNCS constraints8.1992481166E-13
ens_1d_54ZCEEELECTRON MICROSCOPYNCS constraints7.43486957788E-13
ens_1d_55ZCEEELECTRON MICROSCOPYNCS constraints5.55811732665E-13
ens_1d_56ZCEEELECTRON MICROSCOPYNCS constraints7.02959875129E-13
ens_1d_57ZCEEELECTRON MICROSCOPYNCS constraints5.43844569488E-12
ens_1d_58ZCEEELECTRON MICROSCOPYNCS constraints2.67674139398E-11
ens_1d_59ZCEEELECTRON MICROSCOPYNCS constraints6.50338175718E-13
ens_1d_60ZCEEELECTRON MICROSCOPYNCS constraints1.48059815679E-12
ens_1d_61ZCEEELECTRON MICROSCOPYNCS constraints2.64572957897E-11
ens_1d_62ZCEEELECTRON MICROSCOPYNCS constraints3.86070727977E-12
ens_1d_63ZCEEELECTRON MICROSCOPYNCS constraints8.12082696163E-13
ens_1d_64ZCEEELECTRON MICROSCOPYNCS constraints1.03601092352E-11
ens_1d_65ZCEEELECTRON MICROSCOPYNCS constraints6.73915596149E-13
ens_1d_66ZCEEELECTRON MICROSCOPYNCS constraints4.17662996564E-13
ens_1d_67ZCEEELECTRON MICROSCOPYNCS constraints6.04915340962E-13
ens_1d_68ZCEEELECTRON MICROSCOPYNCS constraints6.85295826112E-13
ens_1d_69ZCEEELECTRON MICROSCOPYNCS constraints5.89752334954E-13
ens_1d_70ZCEEELECTRON MICROSCOPYNCS constraints5.66459202695E-13
ens_1d_71ZCEEELECTRON MICROSCOPYNCS constraints4.96545946235E-13
ens_1d_72ZCEEELECTRON MICROSCOPYNCS constraints1.14523566865E-12
ens_1d_73ZCEEELECTRON MICROSCOPYNCS constraints9.41530059272E-13
ens_1d_74ZCEEELECTRON MICROSCOPYNCS constraints2.15357130432E-12
ens_1d_75ZCEEELECTRON MICROSCOPYNCS constraints1.32597082168E-11
ens_1d_76ZCEEELECTRON MICROSCOPYNCS constraints1.13123891124E-11
ens_1d_77ZCEEELECTRON MICROSCOPYNCS constraints5.32086118134E-13
ens_1d_78ZCEEELECTRON MICROSCOPYNCS constraints7.60904017459E-11
ens_1d_79ZCEEELECTRON MICROSCOPYNCS constraints4.8593635189E-13
ens_1d_80ZCEEELECTRON MICROSCOPYNCS constraints7.25385604249E-13
ens_1d_81ZCEEELECTRON MICROSCOPYNCS constraints7.66019258328E-13
ens_1d_82ZCEEELECTRON MICROSCOPYNCS constraints3.00291964948E-12
ens_1d_83ZCEEELECTRON MICROSCOPYNCS constraints2.42034704046E-11
ens_1d_84ZCEEELECTRON MICROSCOPYNCS constraints7.07830158507E-11
ens_1d_85ZCEEELECTRON MICROSCOPYNCS constraints6.42278744169E-13
ens_1d_86ZCEEELECTRON MICROSCOPYNCS constraints2.94190914145E-12
ens_1d_87ZCEEELECTRON MICROSCOPYNCS constraints8.57062628918E-11
ens_1d_88ZCEEELECTRON MICROSCOPYNCS constraints4.32066645181E-13
ens_1d_89ZCEEELECTRON MICROSCOPYNCS constraints5.99659740431E-13
ens_1d_90ZCEEELECTRON MICROSCOPYNCS constraints5.7693154041E-13
ens_1d_91ZCEEELECTRON MICROSCOPYNCS constraints4.31948964612E-13
ens_1d_92ZCEEELECTRON MICROSCOPYNCS constraints3.69788470811E-13
ens_1d_93ZCEEELECTRON MICROSCOPYNCS constraints2.89355484567E-12
ens_1d_94ZCEEELECTRON MICROSCOPYNCS constraints7.76969169668E-13
ens_1d_95ZCEEELECTRON MICROSCOPYNCS constraints5.72489560288E-13
ens_1d_96ZCEEELECTRON MICROSCOPYNCS constraints8.57503169461E-13
ens_1d_97ZCEEELECTRON MICROSCOPYNCS constraints6.21653321627E-11
ens_1d_98ZCEEELECTRON MICROSCOPYNCS constraints1.62493096307E-12
ens_1d_99ZCEEELECTRON MICROSCOPYNCS constraints1.77846897053E-11
ens_1d_100ZCEEELECTRON MICROSCOPYNCS constraints6.50259727432E-13
ens_1d_101ZCEEELECTRON MICROSCOPYNCS constraints7.49785692818E-13
ens_1d_102ZCEEELECTRON MICROSCOPYNCS constraints6.61189306686E-13
ens_1d_103ZCEEELECTRON MICROSCOPYNCS constraints1.28949880623E-12
ens_1d_104ZCEEELECTRON MICROSCOPYNCS constraints6.82132760293E-13
ens_1d_105ZCEEELECTRON MICROSCOPYNCS constraints8.97988554888E-12
ens_1d_106ZCEEELECTRON MICROSCOPYNCS constraints3.82338877489E-13
ens_1d_107ZCEEELECTRON MICROSCOPYNCS constraints7.30278715057E-13
ens_1d_108ZCEEELECTRON MICROSCOPYNCS constraints6.08695001527E-11
ens_1d_109ZCEEELECTRON MICROSCOPYNCS constraints4.39455523599E-11
ens_1d_110ZCEEELECTRON MICROSCOPYNCS constraints7.04313424813E-11
ens_1d_111ZCEEELECTRON MICROSCOPYNCS constraints9.09483773554E-13
ens_1d_112ZCEEELECTRON MICROSCOPYNCS constraints6.54560620273E-12
ens_1d_113ZCEEELECTRON MICROSCOPYNCS constraints3.20499933177E-11
ens_1d_114ZCEEELECTRON MICROSCOPYNCS constraints7.85685122583E-11
ens_1d_115ZCEEELECTRON MICROSCOPYNCS constraints2.92263526228E-13
ens_1d_116ZCEEELECTRON MICROSCOPYNCS constraints2.6475988304E-12
ens_1d_117ZCEEELECTRON MICROSCOPYNCS constraints9.59548085643E-11
ens_1d_118ZCEEELECTRON MICROSCOPYNCS constraints5.79913911356E-13
ens_1d_119ZCEEELECTRON MICROSCOPYNCS constraints7.33225054891E-12
ens_1d_120ZCEEELECTRON MICROSCOPYNCS constraints5.9749302899E-13
ens_1d_121ZCEEELECTRON MICROSCOPYNCS constraints9.63794279893E-12
ens_1d_122ZCEEELECTRON MICROSCOPYNCS constraints6.39364249561E-13
ens_1d_123ZCEEELECTRON MICROSCOPYNCS constraints5.83568815152E-12
ens_1d_124ZCEEELECTRON MICROSCOPYNCS constraints1.9876960143E-11
ens_1d_125ZCEEELECTRON MICROSCOPYNCS constraints2.27786130236E-12
ens_1d_126ZCEEELECTRON MICROSCOPYNCS constraints6.56405344512E-13
ens_1d_127ZCEEELECTRON MICROSCOPYNCS constraints4.99238268695E-12
ens_1d_128ZCEEELECTRON MICROSCOPYNCS constraints5.60720460535E-13
ens_1d_129ZCEEELECTRON MICROSCOPYNCS constraints1.3862128799E-11
ens_1d_130ZCEEELECTRON MICROSCOPYNCS constraints8.71360103116E-13
ens_1d_131ZCEEELECTRON MICROSCOPYNCS constraints7.36220901292E-13
ens_1d_132ZCEEELECTRON MICROSCOPYNCS constraints6.9442381687E-13
ens_1d_133ZCEEELECTRON MICROSCOPYNCS constraints7.5432558852E-11
ens_1d_134ZCEEELECTRON MICROSCOPYNCS constraints8.87816940834E-12
ens_1d_135ZCEEELECTRON MICROSCOPYNCS constraints7.04128305864E-13
ens_1d_136ZCEEELECTRON MICROSCOPYNCS constraints6.38593910107E-11
ens_1d_137ZCEEELECTRON MICROSCOPYNCS constraints4.56179891418E-12
ens_1d_138ZCEEELECTRON MICROSCOPYNCS constraints2.78708063853E-11
ens_1d_139ZCEEELECTRON MICROSCOPYNCS constraints6.64753013652E-11
ens_1d_140ZCEEELECTRON MICROSCOPYNCS constraints8.44165064803E-13
ens_1d_141ZCEEELECTRON MICROSCOPYNCS constraints3.18369678745E-12
ens_1d_142ZCEEELECTRON MICROSCOPYNCS constraints7.34508571531E-13
ens_1d_143ZCEEELECTRON MICROSCOPYNCS constraints4.77356477542E-13
ens_1d_144ZCEEELECTRON MICROSCOPYNCS constraints5.95561500028E-13
ens_1d_145ZCEEELECTRON MICROSCOPYNCS constraints6.6724243651E-13
ens_1d_146ZCEEELECTRON MICROSCOPYNCS constraints6.15708586584E-13
ens_1d_147ZCEEELECTRON MICROSCOPYNCS constraints4.68106483892E-13
ens_1d_148ZCEEELECTRON MICROSCOPYNCS constraints1.50421994051E-12
ens_1d_149ZCEEELECTRON MICROSCOPYNCS constraints8.59976022239E-13
ens_1d_150ZCEEELECTRON MICROSCOPYNCS constraints5.70290452479E-13
ens_1d_151ZCEEELECTRON MICROSCOPYNCS constraints6.1078931844E-13
ens_1d_152ZCEEELECTRON MICROSCOPYNCS constraints9.9107408333E-11
ens_1d_153ZCEEELECTRON MICROSCOPYNCS constraints2.06305366403E-12
ens_1d_154ZCEEELECTRON MICROSCOPYNCS constraints2.25649210502E-11

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