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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9f1c | ||||||||||||||||||
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| タイトル | Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E | ||||||||||||||||||
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キーワード | TRANSLATION / ribosome / NAC / N-terminal acetyltransferase / NatA / NatE / MetAP1 | ||||||||||||||||||
| 機能・相同性 | 機能・相同性情報negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / positive regulation of protein refolding / mitotic sister chromatid cohesion, centromeric / N-terminal methionine Nalpha-acetyltransferase NatE / protein-N-terminal-glutamate acetyltransferase activity / N-terminal protein amino acid acetylation / N-terminal amino-acid Nalpha-acetyltransferase NatA / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development / NatA complex ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / positive regulation of protein refolding / mitotic sister chromatid cohesion, centromeric / N-terminal methionine Nalpha-acetyltransferase NatE / protein-N-terminal-glutamate acetyltransferase activity / N-terminal protein amino acid acetylation / N-terminal amino-acid Nalpha-acetyltransferase NatA / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development / NatA complex / protein N-terminal-methionine acetyltransferase activity / protein N-terminal-serine acetyltransferase activity / positive regulation of cell proliferation involved in heart morphogenesis / protein-N-terminal-alanine acetyltransferase activity / positive regulation of skeletal muscle tissue growth / protein-N-terminal amino-acid acetyltransferase activity / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / cardiac ventricle development / methionyl aminopeptidase / initiator methionyl aminopeptidase activity / histone H4 acetyltransferase activity / acetyltransferase activator activity / N-acetyltransferase activity / heart trabecula morphogenesis / establishment of mitotic sister chromatid cohesion / metalloexopeptidase activity / skeletal muscle tissue regeneration / mitotic sister chromatid cohesion / metalloaminopeptidase activity / laminin receptor activity / glutathione transferase / glutathione transferase activity / aminopeptidase activity / 90S preribosome / ubiquitin ligase inhibitor activity / protein-lysine-acetyltransferase activity / positive regulation of signal transduction by p53 class mediator / phagocytic cup / protein-RNA complex assembly / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / laminin binding / gastrulation / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / MDM2/MDM4 family protein binding / cytosolic ribosome / post-translational protein modification / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / glutathione metabolic process / wound healing / small-subunit processome / protein maturation / platelet aggregation / spindle / cytoplasmic ribonucleoprotein granule / rRNA processing / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / antimicrobial humoral immune response mediated by antimicrobial peptide / heparin binding / protein transport / Inactivation, recovery and regulation of the phototransduction cascade / large ribosomal subunit / ribosomal small subunit assembly / virus receptor activity / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / angiogenesis / large ribosomal subunit rRNA binding / transcription regulator complex / killing of cells of another organism / cytosolic large ribosomal subunit / in utero embryonic development / defense response to Gram-negative bacterium / perikaryon / cytoplasmic translation / cell differentiation / tRNA binding / transcription coactivator activity / mitochondrial inner membrane / negative regulation of translation / postsynaptic density / rRNA binding / protein stabilization / nuclear body / structural constituent of ribosome 類似検索 - 分子機能 | ||||||||||||||||||
| 生物種 | Homo sapiens (ヒト)![]() ![]() | ||||||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
データ登録者 | Yudin, D. / Scaiola, A. / Ban, N. | ||||||||||||||||||
| 資金援助 | 米国, ドイツ, スイス, European Union, 5件
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引用 | ジャーナル: Nature / 年: 2024タイトル: NAC guides a ribosomal multienzyme complex for nascent protein processing. 著者: Alfred M Lentzsch / Denis Yudin / Martin Gamerdinger / Sowmya Chandrasekar / Laurenz Rabl / Alain Scaiola / Elke Deuerling / Nenad Ban / Shu-Ou Shan / ![]() 要旨: Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), ...Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), respectively. Both modifications are strictly cotranslational and essential in higher eukaryotic organisms. The interaction, activity and regulation of these enzymes on translating ribosomes are poorly understood. Here we perform biochemical, structural and in vivo studies to demonstrate that the nascent polypeptide-associated complex (NAC) orchestrates the action of these enzymes. NAC assembles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the active sites of both enzymes for timely sequential processing of the nascent protein. NAC further releases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid protein K (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-terminal acetylation. Our results provide a mechanistic model for the cotranslational processing of proteins in eukaryotic cells. | ||||||||||||||||||
| 履歴 |
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9f1c.cif.gz | 5.4 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9f1c.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9f1c.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/f1/9f1c ftp://data.pdbj.org/pub/pdb/validation_reports/f1/9f1c | HTTPS FTP |
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-関連構造データ
| 関連構造データ | ![]() 50125MC ![]() 9f1bC ![]() 9f1dC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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要素
+RNA鎖 , 6種, 6分子 AHB7ATB8B5A2
+60S ribosomal protein ... , 22種, 22分子 BbBUBZBEBaBGAzBHBcBIBdBJBfBgBMBhBiBkBlBpBTBt
+Small ribosomal subunit protein ... , 9種, 9分子 ArAtAuAZAbAEAnAFAp
+40S ribosomal protein ... , 20種, 20分子 AsAwAxAyAaAcAdAfAgAhAiAAABAkAlADAmAoAGAq
+Large ribosomal subunit protein ... , 11種, 11分子 BABCBPBQBSBBLBOBmBoBr
+Ribosomal protein ... , 12種, 12分子 BVBBBFBYBWAvBRBeAeBNBjAC
+タンパク質 , 7種, 7分子 BXEACtCuDAAjBs
+タンパク質・ペプチド , 1種, 1分子 BK
+N-alpha-acetyltransferase ... , 2種, 2分子 DBDC
+非ポリマー , 8種, 2857分子 














+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
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万見について




Homo sapiens (ヒト)


米国,
ドイツ,
スイス, European Union, 5件
引用












PDBj














































