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基本情報
登録情報 | データベース: PDB / ID: 9f1c | ||||||||||||||||||
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タイトル | Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E | ||||||||||||||||||
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![]() | TRANSLATION / ribosome / NAC / N-terminal acetyltransferase / NatA / NatE / MetAP1 | ||||||||||||||||||
機能・相同性 | ![]() negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / regulation of skeletal muscle fiber development / N-terminal amino-acid Nalpha-acetyltransferase NatA / N-terminal protein amino acid acetylation ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / regulation of skeletal muscle fiber development / N-terminal amino-acid Nalpha-acetyltransferase NatA / N-terminal protein amino acid acetylation / positive regulation of cell proliferation involved in heart morphogenesis / NatA complex / positive regulation of skeletal muscle tissue growth / protein N-terminal-serine acetyltransferase activity / protein N-terminal-methionine acetyltransferase activity / protein-N-terminal-alanine acetyltransferase activity / protein-N-terminal amino-acid acetyltransferase activity / internal protein amino acid acetylation / cardiac ventricle development / N-acetyltransferase activity / histone H4 acetyltransferase activity / methionyl aminopeptidase / initiator methionyl aminopeptidase activity / heart trabecula morphogenesis / skeletal muscle tissue regeneration / establishment of mitotic sister chromatid cohesion / acetyltransferase activator activity / metalloexopeptidase activity / ribosomal subunit / mitotic sister chromatid cohesion / protein-lysine-acetyltransferase activity / laminin receptor activity / protein acetylation / metalloaminopeptidase activity / glutathione transferase / glutathione transferase activity / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / 90S preribosome / chromosome organization / aminopeptidase activity / phagocytic cup / protein-RNA complex assembly / ribosomal small subunit export from nucleus / laminin binding / rough endoplasmic reticulum / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / glutathione metabolic process / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / post-translational protein modification / protein maturation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA / small-subunit processome / wound healing / platelet aggregation / spindle / cytoplasmic ribonucleoprotein granule / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / protein transport / large ribosomal subunit / heparin binding / regulation of translation / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / angiogenesis / perikaryon / defense response to Gram-negative bacterium / transcription regulator complex / killing of cells of another organism / cytosolic large ribosomal subunit / in utero embryonic development / cytoplasmic translation / cell differentiation / transcription coactivator activity / tRNA binding / mitochondrial inner membrane / negative regulation of translation / postsynaptic density / rRNA binding 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
![]() | Yudin, D. / Scaiola, A. / Ban, N. | ||||||||||||||||||
資金援助 | ![]() ![]() ![]()
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![]() | ![]() タイトル: NAC guides a ribosomal multienzyme complex for nascent protein processing. 著者: Alfred M Lentzsch / Denis Yudin / Martin Gamerdinger / Sowmya Chandrasekar / Laurenz Rabl / Alain Scaiola / Elke Deuerling / Nenad Ban / Shu-Ou Shan / ![]() ![]() ![]() 要旨: Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), ...Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), respectively. Both modifications are strictly cotranslational and essential in higher eukaryotic organisms. The interaction, activity and regulation of these enzymes on translating ribosomes are poorly understood. Here we perform biochemical, structural and in vivo studies to demonstrate that the nascent polypeptide-associated complex (NAC) orchestrates the action of these enzymes. NAC assembles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the active sites of both enzymes for timely sequential processing of the nascent protein. NAC further releases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid protein K (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-terminal acetylation. Our results provide a mechanistic model for the cotranslational processing of proteins in eukaryotic cells. | ||||||||||||||||||
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-関連構造データ
関連構造データ | ![]() 50125MC ![]() 9f1bC ![]() 9f1dC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
+RNA鎖 , 6種, 6分子 AHB7ATB8B5A2
+60S ribosomal protein ... , 22種, 22分子 BbBUBZBEBaBGAzBHBcBIBdBJBfBgBMBhBiBkBlBpBTBt
+Small ribosomal subunit protein ... , 9種, 9分子 ArAtAuAZAbAEAnAFAp
+40S ribosomal protein ... , 20種, 20分子 AsAwAxAyAaAcAdAfAgAhAiAAABAkAlADAmAoAGAq
+Large ribosomal subunit protein ... , 11種, 11分子 BABCBPBQBSBBLBOBmBoBr
+Ribosomal protein ... , 12種, 12分子 BVBBBFBYBWAvBRBeAeBNBjAC
+タンパク質 , 7種, 7分子 BXEACtCuDAAjBs
+タンパク質・ペプチド , 1種, 1分子 BK
+N-alpha-acetyltransferase ... , 2種, 2分子 DBDC
+非ポリマー , 8種, 2857分子 














+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
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