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基本情報
登録情報 | データベース: PDB / ID: 9f1c | ||||||||||||||||||
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タイトル | Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E | ||||||||||||||||||
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![]() | TRANSLATION / ribosome / NAC / N-terminal acetyltransferase / NatA / NatE / MetAP1 | ||||||||||||||||||
機能・相同性 | ![]() negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / N-terminal protein amino acid acetylation / NatA complex / protein N-terminal-serine acetyltransferase activity / protein-N-terminal-alanine acetyltransferase activity / protein N-terminal-methionine acetyltransferase activity / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / protein-N-terminal amino-acid acetyltransferase activity / internal protein amino acid acetylation / N-terminal protein amino acid modification / peptidyl-methionine modification / acetyltransferase activator activity / N-acetyltransferase activity / histone H4 acetyltransferase activity / methionyl aminopeptidase / initiator methionyl aminopeptidase activity / heart trabecula morphogenesis / establishment of mitotic sister chromatid cohesion / skeletal muscle tissue regeneration / metalloexopeptidase activity / ribosomal subunit / exit from mitosis / optic nerve development / protein-lysine-acetyltransferase activity / mitotic sister chromatid cohesion / laminin receptor activity / retinal ganglion cell axon guidance / protein acetylation / metalloaminopeptidase activity / glutathione transferase / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / glutathione transferase activity / phagocytic cup / 90S preribosome / chromosome organization / aminopeptidase activity / protein-RNA complex assembly / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / laminin binding / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / glutathione metabolic process / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / protein maturation / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / wound healing / spindle / platelet aggregation / mRNA 5'-UTR binding / cytoplasmic ribonucleoprotein granule / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / protein transport / regulation of translation / large ribosomal subunit / heparin binding / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / angiogenesis / killing of cells of another organism / perikaryon / transcription regulator complex / cytosolic large ribosomal subunit / in utero embryonic development / cytoplasmic translation 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
![]() | Yudin, D. / Scaiola, A. / Ban, N. | ||||||||||||||||||
資金援助 | ![]() ![]() ![]()
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![]() | ![]() タイトル: NAC guides a ribosomal multienzyme complex for nascent protein processing. 著者: Alfred M Lentzsch / Denis Yudin / Martin Gamerdinger / Sowmya Chandrasekar / Laurenz Rabl / Alain Scaiola / Elke Deuerling / Nenad Ban / Shu-Ou Shan / ![]() ![]() ![]() 要旨: Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), ...Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), respectively. Both modifications are strictly cotranslational and essential in higher eukaryotic organisms. The interaction, activity and regulation of these enzymes on translating ribosomes are poorly understood. Here we perform biochemical, structural and in vivo studies to demonstrate that the nascent polypeptide-associated complex (NAC) orchestrates the action of these enzymes. NAC assembles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the active sites of both enzymes for timely sequential processing of the nascent protein. NAC further releases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid protein K (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-terminal acetylation. Our results provide a mechanistic model for the cotranslational processing of proteins in eukaryotic cells. | ||||||||||||||||||
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-関連構造データ
関連構造データ | ![]() 50125MC ![]() 9f1bC ![]() 9f1dC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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集合体
登録構造単位 | ![]()
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要素
+RNA鎖 , 6種, 6分子 AHB7ATB8B5A2
+60S ribosomal protein ... , 22種, 22分子 BbBUBZBEBaBGAzBHBcBIBdBJBfBgBMBhBiBkBlBpBTBt
+Small ribosomal subunit protein ... , 9種, 9分子 ArAtAuAZAbAEAnAFAp
+40S ribosomal protein ... , 20種, 20分子 AsAwAxAyAaAcAdAfAgAhAiAAABAkAlADAmAoAGAq
+Large ribosomal subunit protein ... , 11種, 11分子 BABCBPBQBSBBLBOBmBoBr
+Ribosomal protein ... , 12種, 12分子 BVBBBFBYBWAvBRBeAeBNBjAC
+タンパク質 , 7種, 7分子 BXEACtCuDAAjBs
+タンパク質・ペプチド , 1種, 1分子 BK
+N-alpha-acetyltransferase ... , 2種, 2分子 DBDC
+非ポリマー , 8種, 2857分子 














+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
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