EMDB-50124: Mammalian ternary complex of a translating 80S ribosome, NAC and ...

Basic information

Entry

Database: EMDB / ID: EMD-50124

• Title: Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E
• Map data: homogeneously refined cryo-EM map of the ternary RNC-NAC-NatA/E complex

Sample

• Complex: Ternary complex of a translating ribosome, NAC and NatA/E
  • Complex: Rabbit ribosome-nascent chain complex
    • Protein or peptide: x 76 types
    • RNA: x 6 types
  • Complex: Human NAC heterodimer
    • Protein or peptide: x 2 types
  • Complex: Human NatA/E complex
    • Complex: Human NatA
      • Protein or peptide: x 2 types
    • Complex: Human NatE with a GST tag
      • Protein or peptide: x 1 types
• Protein or peptide: x 2 types
• Ligand: x 8 types

• Keywords: translation / ribosome / N-terminal acetyltransferase / NatA / NatE / NAC

Function / homology

Function:

negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / N-terminal protein amino acid acetylation / NatA complex / protein N-terminal-serine acetyltransferase activity / protein-N-terminal-alanine acetyltransferase activity / protein N-terminal-methionine acetyltransferase activity / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / protein-N-terminal amino-acid acetyltransferase activity / internal protein amino acid acetylation / acetyltransferase activator activity / N-acetyltransferase activity / histone H4 acetyltransferase activity / heart trabecula morphogenesis / establishment of mitotic sister chromatid cohesion / skeletal muscle tissue regeneration / ribosomal subunit / exit from mitosis / optic nerve development / protein-lysine-acetyltransferase activity / mitotic sister chromatid cohesion / laminin receptor activity / retinal ganglion cell axon guidance / protein acetylation / glutathione transferase / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / glutathione transferase activity / phagocytic cup / 90S preribosome / chromosome organization / protein-RNA complex assembly / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / laminin binding / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / glutathione metabolic process / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / wound healing / spindle / mRNA 5'-UTR binding / cytoplasmic ribonucleoprotein granule / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / protein transport / regulation of translation / large ribosomal subunit / heparin binding / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / angiogenesis / killing of cells of another organism / perikaryon / transcription regulator complex / cytosolic large ribosomal subunit / in utero embryonic development / cytoplasmic translation / cell differentiation / transcription coactivator activity / tRNA binding / mitochondrial inner membrane / postsynaptic density / negative regulation of translation / protein stabilization / rRNA binding / nuclear body / ribosome

Domain/homology:

Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain / Nascent polypeptide-associated complex subunit alpha / NAC A/B domain superfamily / NAC domain / NAC A/B domain profile. / NAC / Nascent polypeptide-associated complex subunit alpha-like, UBA domain / HYPK UBA domain / N-terminal acetyltransferase A, auxiliary subunit / N-terminal acetyltransferase A, auxiliary subunit / : / N-acetyltransferase Ard1-like / Glutathione S-transferase, C-terminal domain / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / : / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Acetyltransferase (GNAT) family / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Glutathione S-transferase, N-terminal domain / Glutathione transferase family / 50S ribosomal protein L10, insertion domain superfamily / Tetratricopeptide repeat / Ribosomal protein L23 / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Glutathione S-transferase, C-terminal / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / metallochaperone-like domain / TRASH domain / Glutathione S-transferase, C-terminal-like / Soluble glutathione S-transferase C-terminal domain profile. / Soluble glutathione S-transferase N-terminal domain profile. / Glutathione S-transferase, N-terminal / GNAT domain / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Glutathione S-transferase, C-terminal domain superfamily / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S17e, conserved site / : / Ribosomal protein S27a

Component:

Small ribosomal subunit protein eS32 / 60S ribosomal protein L36a-like / Ribosomal protein S14 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL15 / 40S ribosomal protein S24 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Ribosomal protein L19 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein eL20 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein uS19 / Ribosomal protein L32 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Glutathione S-transferase class-mu 26 kDa isozyme / Ubiquitin-ribosomal protein eL40 fusion protein / Transcription factor BTF3 / N-alpha-acetyltransferase 10 / Nascent polypeptide-associated complex subunit alpha / N-alpha-acetyltransferase 15, NatA auxiliary subunit / N-alpha-acetyltransferase 50 / Large ribosomal subunit protein eL37

• Biological species: Oryctolagus cuniculus (rabbit) / Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.01 Å
• Authors: Yudin D / Scaiola A / Ban N

Funding support

United States, Germany, Switzerland, European Union, 5 items

Organization	Grant number	Country
National Institutes of Health/Office of the Director	R35 GM136321	United States
German Research Foundation (DFG)	SFB969/A01 and A07	Germany
Swiss National Science Foundation	310030_212308	Switzerland
Swiss National Science Foundation	51NF40-205601	Switzerland
European Research Council (ERC)	Synergy Grant 101072047	European Union

• Citation: Journal: Nature / Year: 2024; Title: NAC guides a ribosomal multienzyme complex for nascent protein processing.; Authors: Alfred M Lentzsch / Denis Yudin / Martin Gamerdinger / Sowmya Chandrasekar / Laurenz Rabl / Alain Scaiola / Elke Deuerling / Nenad Ban / Shu-Ou Shan / ; Abstract: Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), respectively. Both modifications are strictly cotranslational and essential in higher eukaryotic organisms. The interaction, activity and regulation of these enzymes on translating ribosomes are poorly understood. Here we perform biochemical, structural and in vivo studies to demonstrate that the nascent polypeptide-associated complex (NAC) orchestrates the action of these enzymes. NAC assembles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the active sites of both enzymes for timely sequential processing of the nascent protein. NAC further releases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid protein K (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-terminal acetylation. Our results provide a mechanistic model for the cotranslational processing of proteins in eukaryotic cells.

History

Deposition	Apr 18, 2024	-
Header (metadata) release	Aug 21, 2024	-
Map release	Aug 21, 2024	-
Update	Oct 2, 2024	-
Current status	Oct 2, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_50124.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: homogeneously refined cryo-EM map of the ternary RNC-NAC-NatA/E complex
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.275
Minimum - Maximum	-0.4192591 - 1.3600999
Average (Standard dev.)	0.002097521 (±0.054865252)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 560 560 560 Spacing 560 560 560
Cell	A=B=C: 593.6 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_50124_msk_1.map

Mask #2

• File: emd_50124_msk_2.map

Additional map: main map lowpass filtered to 8 A resolution

• File: emd_50124_additional_1.map
• Annotation: main map lowpass filtered to 8 A resolution

Additional map: main map lowpass filtered to estimated local resolution

• File: emd_50124_additional_2.map
• Annotation: main map lowpass filtered to estimated local resolution

Additional map: main map lowpass filtered to 6 A resolution

• File: emd_50124_additional_3.map
• Annotation: main map lowpass filtered to 6 A resolution

Half map: halfmap A

• File: emd_50124_half_map_1.map
• Annotation: halfmap A

Half map: halfmap B

• File: emd_50124_half_map_2.map
• Annotation: halfmap B

Sample components

Entire : Ternary complex of a translating ribosome, NAC and NatA/E

• Entire: Name: Ternary complex of a translating ribosome, NAC and NatA/E

Components

• Complex: Ternary complex of a translating ribosome, NAC and NatA/E
  • Complex: Rabbit ribosome-nascent chain complex
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: Nascent chain
    • Protein or peptide: 60S ribosomal protein L14
    • Protein or peptide: Large ribosomal subunit protein eL20
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Large ribosomal subunit protein uL13
    • RNA: 18S rRNA
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: Ribosomal protein S27a
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Small ribosomal subunit protein eS26
    • Protein or peptide: Small ribosomal subunit protein RACK1
    • Protein or peptide: 40S ribosomal protein S29
    • RNA: mRNA
    • Protein or peptide: Large ribosomal subunit protein uL22
    • RNA: P-site tRNA
    • Protein or peptide: Large ribosomal subunit protein eL13
    • Protein or peptide: Large ribosomal subunit protein eL18
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: Ribosomal protein L24
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: S10_plectin domain-containing protein
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: 40S ribosomal protein eS17
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Ribosomal_L23eN domain-containing protein
    • Protein or peptide: 40S ribosomal protein S19
    • Protein or peptide: Small ribosomal subunit protein eS21
    • Protein or peptide: Ribosomal protein S15a
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: Large ribosomal subunit protein eL34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39-like
    • Protein or peptide: Ubiquitin-ribosomal protein eL40 fusion protein
    • Protein or peptide: Large ribosomal subunit protein eL42
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: Large ribosomal subunit protein eL28
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: 60S ribosomal protein L12
    • RNA: 28S rRNA
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: Ribosomal Protein uL30
  • Complex: Human NAC heterodimer
    • Protein or peptide: Nascent polypeptide-associated complex subunit alpha
    • Protein or peptide: Transcription factor BTF3
  • Complex: Human NatA/E complex
    • Complex: Human NatA
      • Protein or peptide: N-alpha-acetyltransferase 10
      • Protein or peptide: N-alpha-acetyltransferase 15, NatA auxiliary subunit
    • Complex: Human NatE with a GST tag
      • Protein or peptide: Glutathione S-transferase class-mu 26 kDa isozyme,N-alpha-acetyltransferase 50
• Protein or peptide: Small ribosomal subunit protein uS10
• Protein or peptide: Large ribosomal subunit protein uL18
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: UNKNOWN ATOM OR ION
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: SPERMIDINE
• Ligand: SPERMINE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: water

Supramolecule #1: Ternary complex of a translating ribosome, NAC and NatA/E

• Supramolecule: Name: Ternary complex of a translating ribosome, NAC and NatA/E; type: complex / ID: 1 / Parent: 0 ; Macromolecule list: #20, #16, #21, #26, #31, #35, #40, #44, #48, #52, #56, #60, #63, #66, #69, #72, #75, #78, #81, #84, #87, #1, #4, #7, #10, #13, #25, #17, #22, #27, #32, #36, #41, #45, #49, #53, #57, #61, #64, #67, #70, #73, #76, #79, #82, #85, #88, #2, #5, #8, #11, #30, #18, #23, #28, #33, #37, #42, #46, #50, #54, #58, #62, #65, #68, #71, #74, #77, #80, #83, #86, #89, #3, #6, #9, #12, #15, #39, #19, #24, #29, #34, #38, #43, #47, #55, #59
• Molecular weight: Theoretical: 3.46 MDa

Supramolecule #2: Rabbit ribosome-nascent chain complex

• Supramolecule: Name: Rabbit ribosome-nascent chain complex / type: complex / ID: 2 / Parent: 1 ; Macromolecule list: #16, #21, #26, #31, #35, #40, #44, #48, #52, #63, #66, #69, #72, #75, #78, #81, #84, #87, #1, #4, #7, #10, #13, #25, #17, #22, #27, #32, #36, #41, #45, #49, #53, #57, #61, #64, #67, #70, #73, #76, #79, #82, #85, #88, #2, #5, #8, #11, #30, #18, #23, #28, #33, #37, #42, #46, #50, #54, #58, #62, #65, #68, #71, #74, #77, #80, #83, #86, #89, #3, #9, #12, #15, #39, #24, #29, #34, #38, #43, #47, #55, #59
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)

Supramolecule #3: Human NAC heterodimer

• Supramolecule: Name: Human NAC heterodimer / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #20, #6
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #4: Human NatA/E complex

• Supramolecule: Name: Human NatA/E complex / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #56, #60, #19

Supramolecule #5: Human NatA

• Supramolecule: Name: Human NatA / type: complex / ID: 5 / Parent: 4 / Macromolecule list: #60, #19
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #6: Human NatE with a GST tag

• Supramolecule: Name: Human NatE with a GST tag / type: complex / ID: 6 / Parent: 4 / Macromolecule list: #56
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Large ribosomal subunit protein uL22

• Macromolecule: Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #2: 40S ribosomal protein eS17

• Macromolecule: Name: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.552119 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #3: Large ribosomal subunit protein eL42

• Macromolecule: Name: Large ribosomal subunit protein eL42 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.489991 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QT(MLZ)PIFRKKA KTTKKIVLRL ECVEPN CRS KRMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: 60S ribosomal protein L36a-like

Macromolecule #5: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.670619 KDa

Sequence

String:

(SAC)LVIPEKFQH ILRVLNTNID GRRKIAFAIT AIKGVGRRYA HVVLRKADID LTKRAGELTE DEVERVITIM QNPRQY KIP DWFLNRQKDV KDGKYSQVLA NGLDNKLRED LERLKKIRAH RGLRHFWGLR VRGQHTKTTG RRGRTVGVSK KK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #6: Transcription factor BTF3

• Macromolecule: Name: Transcription factor BTF3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.724037 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKETIMNQEK LAKLQAQVRI GGKGTARRKK KVVHRTATAD DKKLQFSLKK LGVNNISGIE EVNMFTNQGT VIHFNNPKVQ ASLAANTFT ITGHAETKQL TEMLPSILNQ LGADSLTSLR RLAEALPKQS VDGKAPLATG EDDDDEVPDL VENFDEASKN E AN

UniProtKB: Transcription factor BTF3

Macromolecule #7: Large ribosomal subunit protein eL13

• Macromolecule: Name: Large ribosomal subunit protein eL13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.331723 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #8: Ribosomal_L23eN domain-containing protein

• Macromolecule: Name: Ribosomal_L23eN domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.768246 KDa

Sequence

String:

MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #9: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #10: Large ribosomal subunit protein eL18

• Macromolecule: Name: Large ribosomal subunit protein eL18 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.699688 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRVQEVPKLK VCALRVTSRA RSRILKAGGK ILTFDQLALD SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

Macromolecule #11: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.104579 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYL(NMM)GGA GVGSMT KIY GGRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #12: Large ribosomal subunit protein eL28

• Macromolecule: Name: Large ribosomal subunit protein eL28 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.713501 KDa

Sequence

String:

(SAC)AHLQWMVVR NCSSFLIKRN KQTYSTEPNN LKARNSFRYN GLIHRKTVGV EPAADGKGVV VVMKRRSGQR KPATSY VRT TINKNARATL SSIRHMIRKN KYRPDLRMAA IRRASAILRS QKPVMVKRKR TRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #13: Ribosomal protein L19

• Macromolecule: Name: Ribosomal protein L19 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Ribosomal protein L19

Macromolecule #14: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #15: 60S acidic ribosomal protein P0

• Macromolecule: Name: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.380504 KDa

Sequence

String:

MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #16: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCILKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

Macromolecule #17: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.756909 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGA VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #18: Ribosomal protein S15a

• Macromolecule: Name: Ribosomal protein S15a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #19: N-alpha-acetyltransferase 15, NatA auxiliary subunit

• Macromolecule: Name: N-alpha-acetyltransferase 15, NatA auxiliary subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 106.506273 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRARGIQRPT STSSLVAAAM PAVSLPPKEN ALFKRILRCY EHKQYRNGLK FCKQILSNP KFAEHGETLA MKGLTLNCLG KKEEAYELVR RGLRNDLKSH VCWHVYGLLQ RSDKKYDEAI KCYRNALKWD K DNLQILRD LSLLQIQMRD LEGYRETRYQ LLQLRPAQRA SWIGYAIAYH LLEDYEMAAK ILEEFRKTQQ TSPDKVDYEY SE LLLYQNQ VLREAGLYRE ALEHLCTYEK QICDKLAVEE TKGELLLQLC RLEDAADVYR GLQERNPENW AYYKGLEKAL KPA NMLERL KIYEEAWTKY PRGLVPRRLP LNFLSGEKFK ECLDKFLRMN FSKGCPPVFN TLRSLYKDKE KVAIIEELVV GYET SLKSC RLFNPNDDGK EEPPTTLLWV QYYLAQHYDK IGQPSIALEY INTAIESTPT LIELFLVKAK IYKHAGNIKE AARWM DEAQ ALDTADRFIN SKCAKYMLKA NLIKEAEEMC SKFTREGTSA VENLNEMQCM WFQTECAQAY KAMNKFGEAL KKCHEI ERH FIEITDDQFD FHTYCMRKIT LRSYVDLLKL EDVLRQHPFY FKAARIAIEI YLKLHDNPLT DENKEHEADT ANMSDKE LK KLRNKQRRAQ KKAQIEEEKK NAEKEKQQRN QKKKKDDDDE EIGGPKEELI PEKLAKVETP LEEAIKFLTP LKNLVKNK I ETHLFAFEIY FRKEKFLLML QSVKRAFAID SSHPWLHECM IRLFNTAVCE SKDLSDTVRT VLKQEMNRLF GATNPKNFN ETFLKRNSDS LPHRLSAAKM VYYLDPSSQK RAIELATTLD ESLTNRNLQT CMEVLEALYD GSLGDCKEAA EIYRANCHKL FPYALAFMP PGYEEDMKIT VNGDSSAEAE ELANEI

UniProtKB: N-alpha-acetyltransferase 15, NatA auxiliary subunit

Macromolecule #20: Nascent polypeptide-associated complex subunit alpha

• Macromolecule: Name: Nascent polypeptide-associated complex subunit alpha / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.85748 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MGSSHHHHHH SSGLEVLFQG PSGMPGEATE TVPATEQELP QPQAETGSGT ESDSDESVPE LEEQDSTQAT TQQAQLAAAA EIDEEPVSK AKQSRSEKKA RKAMSKLGLR QVTGVTRVTI RKSKNILFVI TKPDVYKSPA SDTYIVFGEA KIEDLSQQAQ L AAAEKFKV QGEAVSNIQE NTQTPTVQEE SEEEEVDETG VEVKDIELVM SQANVSRAKA VRALKNNSND IVNAIMELTM

UniProtKB: Nascent polypeptide-associated complex subunit alpha

Macromolecule #21: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.871418 KDa

Sequence

String:

MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #22: Ribosomal protein L23

• Macromolecule: Name: Ribosomal protein L23 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #23: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.860666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK Q(HY3)NSAIRKCV RVQLIK NGK KITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #25: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #26: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.643057 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #27: Ribosomal protein L24

• Macromolecule: Name: Ribosomal protein L24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #28: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.107924 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK K

UniProtKB: 40S ribosomal protein S24

Macromolecule #30: Small ribosomal subunit protein eS21

• Macromolecule: Name: Small ribosomal subunit protein eS21 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.181374 KDa

Sequence

String:

(AME)QNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKSD GIV SQNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #31: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #32: Small ribosomal subunit protein uS2

• Macromolecule: Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 32.838828 KDa

Sequence

String:

(SAC)GALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVI SSR NTGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYV DI AIPCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPEE IEKEEQAAAE KAVTKEEFQG EWTAPAPE F TATQPEVADW SEGVQVPSVP IQQFPTEDWS AQPATEDWSA APTAQATEWV GTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #33: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.645028 KDa

Sequence

String:

PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #35: Nascent chain

• Macromolecule: Name: Nascent chain / type: protein_or_peptide / ID: 35 ; Details: Nascent polypeptide chain linked to the P-site tRNA. The nascent chain was translated by programming RRL in vitro translation system with a truncated mRNA that would result in translation termination at the 3' end of the mRNA as described in the methods section of the manuscript. The nascent chain was modeled as a polyalanine chain.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 2.486056 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #36: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #37: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #38: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.103855 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGN(V5N)QH IGKPSTIRRD APAGRKVG L IAARRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #39: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #40: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.870549 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #41: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.285426 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG GRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW LPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG AALKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

Macromolecule #42: Ribosomal protein L26

• Macromolecule: Name: Ribosomal protein L26 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #43: Ribosomal protein L3

• Macromolecule: Name: Ribosomal protein L3 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.120996 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #44: Large ribosomal subunit protein eL20

• Macromolecule: Name: Large ribosomal subunit protein eL20 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.749391 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KSPAPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQVMKV EEMAAARCRR PAVKQFHDSR IRFPLPHRVL R RQHKPRFS AKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #45: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.146607 KDa

Sequence

String:

MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #46: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #47: Large ribosomal subunit protein uL4

• Macromolecule: Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.299699 KDa

Sequence

String:

(AYA)CARPLISVY SEKGESSGKN VTLPAVFKAP IRPDIVNFVH TNLRKNNRQP YAVSELAGHQ TSAESWGTGR AVARIP RVR GGGTHRSGQG AFGNMCRGGR MFAPTKTWRR WHRRVNTTQK RYAICSALAA SALPALVMSK GHRIEEVPEL PLVVEDK VE GYKKTKEAVL LLKKLKAWND IKKVYASQRM RAGKGKMRNR RRIQRRGPCV IYNEDNGIVK AFRNIPGITL LNVTKLNI L KLAPGGHVGR FCIWTESAFR KLDDLYGTWR KAASLKSNYN LPMHKMLNTD LSRILKSPEI QRALRAPRKK IHRRVLKKN PLKNLRIMLK LNPYAKTMRR NTILRQARNH KLRVERAAAA LAAKSDPKEA PAKKKPVVGK KKKPVVGRKA AAAKKPAADK KAADKRAGP EDKKPAA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #48: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #49: 40S ribosomal protein S4

• Macromolecule: Name: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

Macromolecule #50: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.635551 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGM(V5N)H HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLD KLW TLVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #51: Large ribosomal subunit protein uL18

• Macromolecule: Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.481828 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #52: Large ribosomal subunit protein uL13

• Macromolecule: Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.533299 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #53: Ribosomal protein S5

• Macromolecule: Name: Ribosomal protein S5 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #54: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 26.721727 KDa

Sequence

String:

MAKS(MLZ)NHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALV KPK EVKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGK AQ AETKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPK G AQPPAKAP

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #55: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 33.028336 KDa

Sequence

String:

MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #56: Glutathione S-transferase class-mu 26 kDa isozyme,N-alpha-acetylt...

• Macromolecule: Name: Glutathione S-transferase class-mu 26 kDa isozyme,N-alpha-acetyltransferase 50; type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO / EC number: glutathione transferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.529668 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKER AEISMLEGAV LDIRYGVSRI AYSKDFETLK VDFLSKLPEM LKMFEDRLCH KTYLNGDHVT HPDFMLYDAL D VVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK GSGSGSENLY FQGAMVMKGS RI ELGDVTP HNIKQLKRLN QVIFPVSYND KFYKDVLEVG ELAKLAYFND IAVGAVCCRV DHSQNQKRLY IMTLGCLAPY RRL GIGTKM LNHVLNICEK DGTFDNIYLH VQISNESAID FYRKFGFEII ETKKNYYKRI EPADAHVLQK NLKVPSGQNA DVQK TDN

UniProtKB: Glutathione S-transferase class-mu 26 kDa isozyme, N-alpha-acetyltransferase 50

Macromolecule #57: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #58: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.807065 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #59: Ribosomal Protein uL30

• Macromolecule: Name: Ribosomal Protein uL30 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.201836 KDa

Sequence

String:

MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #60: N-alpha-acetyltransferase 10

• Macromolecule: Name: N-alpha-acetyltransferase 10 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO; EC number: N-terminal amino-acid Nalpha-acetyltransferase NatA
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.47958 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MNIRNARPED LMNMQHCNLL CLPQNFQMKY YFYHGLSWPQ LSYIAEDENG KIVGYVLAKM EEDPDDVPHG HITSLAVKRS HRRLGLAQK LMDQASRAMI ENFNAKYVSL HVRKSNRAAL HLYSNTLNFQ ISEVEPKYYA DGEDAYAMKR DLTQMADELR R HLELKEKG RHVVLGAIEN KVESKGNSPP SSGEACREEK GLAAEDSGGD SKDLSEVSET TESTDVKDSS EASDSAS

UniProtKB: N-alpha-acetyltransferase 10

Macromolecule #61: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 47.356172 KDa

Sequence

String:

EPITNRVFQA LTSSDFKQQD GGSFSLSRTH LSGFRSRPRR TTIASAATRP PPIPSRGGSA PTGTPEGAGA SRSSRTVPRL PPQPSGPPR APRHPGFQRA LPSLIRPEPG VRAPLLASAG TRFKRPASQA VKALSARGLL QPQVQVGPRA NRVPQRREPR Q HLSGHAPG ASEGGSASPT YWSLGITSWD ALGFPLRLGG SSGFRSSVAL FRPLSFRRGD LPRSREIWAS SSAGAQPGEA MF SSSAKIV KPNGEKPDEF ESGISQALLE LEMNSDLKAQ LRELNITAAK EIEVGGGRKA IIIFVPVPQL KSFQKIQVRL VRE LEKKFS GKHVVFIAQR RILPKPTRKS RTKNKQKRPR SRTLTAVHDA ILEDLVFPSE IVGKRIRVKL DGSRLIKVHL DKAQ QNNVE HKVETFSGVY KKLTGKDVNF EFPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #62: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #64: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #65: Ribosomal protein L32

• Macromolecule: Name: Ribosomal protein L32 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Ribosomal protein L32

Macromolecule #66: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #67: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #68: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #69: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #70: S10_plectin domain-containing protein

• Macromolecule: Name: S10_plectin domain-containing protein / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #71: Large ribosomal subunit protein eL34

• Macromolecule: Name: Large ribosomal subunit protein eL34 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.125816 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #72: Ribosomal protein S27a

• Macromolecule: Name: Ribosomal protein S27a / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #73: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #74: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.566599 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #75: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.498884 KDa

Sequence

String:

MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #76: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #77: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.263834 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #78: Small ribosomal subunit protein eS26

• Macromolecule: Name: Small ribosomal subunit protein eS26 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

Macromolecule #79: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #80: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #81: Small ribosomal subunit protein RACK1

• Macromolecule: Name: Small ribosomal subunit protein RACK1 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #82: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPS(IAS)S TRRKGGRRGR RL

UniProtKB: Ribosomal protein S14

Macromolecule #83: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #84: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #85: 40S ribosomal protein uS19

• Macromolecule: Name: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.049182 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #86: 60S ribosomal protein L39-like

• Macromolecule: Name: 60S ribosomal protein L39-like / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.455775 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #88: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 88 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 19.213465 KDa

Sequence

String:

SARVAPRFPF PVVAPRGEDR HSSAAAMPSK GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERF AGVDIRVRVK GGGHVAQIYA IRQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG G PGARARYQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #89: Ubiquitin-ribosomal protein eL40 fusion protein

• Macromolecule: Name: Ubiquitin-ribosomal protein eL40 fusion protein / type: protein_or_peptide / ID: 89 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.800474 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICR(M3L)C YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

Macromolecule #4: P-site tRNA

• Macromolecule: Name: P-site tRNA / type: rna / ID: 4 ; Details: Peptidyl-tRNA bound at the P-site in the stalled ribosome-nascent chain complex. The residues of the tRNA were modeled as N apart from the CCA at the 3' end.; Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.209544 KDa

Sequence

String:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)CCA

Macromolecule #24: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 24 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 1.5575195 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGUGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G (A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUC AACCC GGCGGCGUGG UCCGGCCGUG CCGGCGGUCU CGGCGGAUCU UUCCCGCUCC CCGUUCCUCC CGACCCCUCC ACCCG CUCC CCCUCCCCCG CCGGUCUUCU CCCUUCACGG GGGGUGGGUC GGCGGGUGCG GGGGUGGGCG GGCGGGGCCG GGGGUG GGG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCGCAUU UCCACCGCGG CGGUGCGCCG CGACCGG CU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGGACGCC UCGCCGCCGU CGUCCGCGGC GGCGGCGG G CGCGACCCCU CCCCGGGUGU UACAGCCCCG CCCCGGCAGC AGCAGCUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCU GUGUCGCCGC GCUCUCCCCC CUCCCGGCGC UCCCCCCCGC GGGGGUCCCC CGCGAGGGGG CUCCCCCCGC GGGGGCGCGC CGGUGUCGC CGCCCUCGCG GGCGCGGCAC GGGGGGGCCG GGCCGCCCCU CCCACGGCGC GACCGCUCUC CCGGCCCCCG C CGCCGCCU CUUCGGGCGU GCGGCGGGGG UGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGUGUCACGC CGUCGGGCUC GG GGGAGAA GUCGGCCACG GCGCGUCGCG CGCCUCCGCG UCCCCCCCUC GCCGUCUCCG GGCGGCGGGG GGGGGUUUCG GCG CGGUGU UCUCGGCGCG CGCGCGCGAG CGAGCGCACG GGGUCGGCGG CGAUGUCGGC UACCCACCCG ACCC(OMG)UCUUG (1MA)AAC(A2M)CGGA CCAAGGAGU(OMC) UAACACGUGC GCGAGUCGGG GGCUCGCACG AAAGCCGCCG UGGCGCAAU GAAGGUGAAG GCCGGCGGCG GCUUCCCGCC GUCGUCGGCC GAGGUGGGAU CCCGAGGCCC UUCCAGUCCG CCGAGGGCGC ACCACCGGC CCGUCUCGCC CGCCGCGCCG GGGAGGUGGA GCACGAGCGC ACGUGUUAGG ACCC(OMG)A(A2M)AGA UGG UGA(A2M)C(PSU) AUGCCUGGGC AGGGCGAAGC CAGAGGAAAC UCUGGUGGAG GUCCG(PSU)AGCG GUCCUGACGU GC AAAUCGG UCGUCCGACC UGGGUAUAG(OMG) GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUCCCUC CGAAGUUUC CC(PSU)CAGGA(PSU)A GCUGGCGCUC UCGCAACCCC GCAGUUUUAU CCGGUAAAGC GAA(PSU)GAUUAG AGGUCUUG G GGCCGAAACG AUCUCAACC(PSU) A(PSU)(PSU)CUCAAAC UU(PSU)AAAUGGG UAAGAAGCCC GGCUCGCUGG CGU GGAGCC GGGCGUGGAA UGCGAGUGCC UAGUGGGCCA C(PSU)U(PSU)UGGUAA GC(A2M)GAACUGG CG(OMC)UGCGG G AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGCCGACGCU CAUCAGACCC CAGAAAAGGU GUUGGUUGAU AUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGCC CUGAAAAUGG AUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGUCGCUG GCAGUCGGCA GAGCGAGAAA GAGAGUGGAG CGCGCCGCGG G ACUCCCCC CGCGGGGGUC CCCGGCCACC CCCCCCCCGC GGACGCUACG CCGCGACGAG UAGGAGGGCC GCUGCGGUGA GC CUUGAAG CCUAGGGCGC GGGCCCGGGU GGAGCCGCCG CAGGUGCAGA U(OMC)UUGGUGGU AGU(A2M)(OMG)(OMC)A AA UAUUCAAACG AGAACUUUGA AGGCCGAAGU GG(A2M)GAAGGGU UCCAUG(OMU)GAA CAG(OMC)A(OMG)UUGA AC AUGGGUC AGUCGGUCCU GAGAGAUGGG CGAGCGCCGU UCCGAAGGGA CGGGCGAUGG CCUCCGUUGC CCUCAGCCGA (PSU)CGAAAGGG AGUCGGGUUC AGAUCCCCGA AUCCGGAGUG GCGGAGAUGG GCGCCGCGAG GCGUCCAGUG CGGUAAC GC GACCGAUCCC GGAGAAGCCG GCGGGAGCCC CGGGGAGAGU UCUCUU(PSU)UCU UUGUGAAGGG CAGGGCGCCC UGGA AUGGG UUCGCCCCGA GAGAGGGGCC CGUGCCUUGG AAAGCGUCGC GGUUCCGGCG GCGUCCGGUG AGCUCUCGCU GGCCC UUGA AAAUCCGGGG GAGAGGGUGU AAAUCUCGCG CCGGGCCGUA CCC(A2M)UAUCCG CAGCAGGUCU (OMC)CAAGGUG A AC(A2M)GCCUCUG G(OMC)AUGUUGGA ACAA(OMU)GUAGG UAAGGGAAGU CGGCAAGC(OMC)G GAUCCGUAAC UUC (OMG)GGAUA AGGAUUGGCU CUAAGGGCUG GGUCGGUCGG GCUGGGGCGC GAAGCGGGGC UGGGCGCGCG CCGCGGCUGG ACGAGGCGC CGCCGCCCCC CCCACGCCCG GGGCGCCCUC CCCCGGCCGG GUCCGCUCCC UCGCGCGCGC GUGCGCCGUG G CGCGCGCC GCGCGCGCGG CCGAGCCCCG UCUCCCUCCC CCCCUCUCCC CUCCCGGGGC GGGGUGCGGG GAGGGGCGGG CG GGGCCGU CGCUCGCCGC GCGUGCGCGU CGCUGCGCGU GCGCCGCGCG GGGGGUCCGG CGGGCCUCCG GACGGGGGCU CCG GGCACC CGGGCACCCG GGGGGCCGGC GGCGGCGGCG ACUCUGGACG CGAGCCGGGC CCUUCCCGUG GAUCGCCCCA GCUG CGGCG GGCGUCGCGG CCGCCCUCGG GGAGCCCGGC GGGCGCCGCG UGCGCGCGCG UGCGCGCCCG GGGUCGGGGG GGUGC GAGG CAGGGGAGGC CCUCUCUCCU CCUCCCCCAC CCCGGCGCCG CGGCGCCGCG CGUGCGCGCG CGGUCUCCCC CCGCCG GGU CCGCCCCCGG GGCCGCGGUU CCGCGCGGCG CCUCGCCUCG GCCGGCGCCU AGCAGCCGAC UUAGAACUGG UGCGGAC CA GG(OMG)GAAUCCG AC(PSU)G(PSU)UUAAU UAAAACAAAG CAUCGCGAAG GCCCGCGGCG GGUGUUGACG CGAUGU GAU U(PSU)CUGCC(OMC)AG UGCUCUGAAU G(PSU)CA(A2M)AGUGA (A2M)GAAAU(PSU)CAA (PSU)GAAGCGCG G(OMG)UAAACGGC GGGAG(PSU)A(A2M)C(PSU) A(PSU)GAC(PSU)C(PSU)CU UAAGGUAGC(5MC) AA(A2M)U GCCUC (OMG)UCAUCUAAU UAGUGA(OMC)GCG CAUGAA(UY1)GGA (PSU)GA(A2M)CGAG(A2M)U UCCCACUGU (OMC)CC(PSU)ACCUAC (PSU)A(PSU)CCAGCGA AACCAC(A2M)G(OMC)C AAGGGAACGG GCU(PSU)GG(OMC)G G AAUCAGCGG(OMG) GAAAGAAGAC CCUGUUGAGC (PSU)UGAC(OMU)CUAG UCUGGCACGG UGAA(OMG)AGACA UGA GAGGUG UAGAAUAAGU GGGAGGCCCC CGGCGCCCUC CCGCCCCCGC GAGGGGGCGG GGCGGGGUCC GCCGGCCUUG CGGG CCGCC GGUGAAAUAC CACUACUCUU AUCGUUUUUU CACUGACCCG GUGAGGCGGG GGGGCGAGCC CCGAGGGGCU CUCGC UUCU GGCGCCAAGC GCCGGGCCCC GUCGGUCCGC GCGGGCCGGC GGCGGCCGGG CGCGACCCGC UCCGGGGACA GUGCCA GGU GGGGAGUUUG ACUG(OMG)GGCGG UACACCUGUC AAACGGUA(6MZ)C GCAGG(OMU)(OMG)UCC UAAGGCGAGC U CAGGGAGG ACAGAAACCU CCCGUGGAGC AGAAGGGCAA AAGCUCGCUU GA(PSU)CU(PSU)GA(PSU)U UUCAG(OMU) ACG AA(PSU)ACAGACC GUGAAAGCGG GGCCUCACGA UCCUUCUGAG CUU(PSU)UGGGUU U(PSU)AAGCAGGA (OMG) GUGUCAGA AAAGUUACCA CAG(OMG)GAUAAC UGGC(PSU)UGUGG CGGCCAAGCG U(PSU)CA(PSU)AGCGA CG (PSU)CGCUUU UUGA(PSU)CCUU(5MC) GAUGUCGG(OMC)(PSU) CUUCCUAUCA UUG(PSU)GAAGCA GAAUUCACC AAGCGUU(OMG)GA U(OMU)(OMG)(PSU)UCACCC ACUAAUAGGG AACG(PSU)G(A2M)GCU GGG(UR3)U(PSU)AG A (OMC)CGUCGUGAG ACAGGU(PSU)AGU UUUACCCUAC UGAUG(A2M)UGUG (PSU)UG(PSU)UGCCAU GGUA(A2M) UCCU GCUCAGUACG AGAGGAACCG CAG(OMG)U(OMU)CA(OMG)A CAU(PSU)UGGUGU A(PSU)(OMG)UGCUUGG CU GAGGAGC CAAUGGGGCG AAGCUACCA(PSU) CUGUGGGAUU AUGAC(PSU)GAAC GCCUCUAAGU CAGAAUCCCG CCCAG GCGG AACGAUACGG CAGCGCCGAA GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCGUC CCCGCCGGCG GCCCGC GUC GUGCGUCCGC CUCGGCGGCG UGCGGCGCGC CCCCCGCCGC GCGUCGGGAC CGGGGUCCGG UGCGGAGAGC CCUUCGU CC CGGGAAAUGG GGCGCGGCCG GAAAGGGGGC CGCCCUCUCG CCCGUCACGC ACCGCACGUU CGUGGGGAAC CUGGCGCU A AACCA(PSU)UCGU AGACGACCUG CUUCUGGGUC GGGG(PSU)UUCGU ACG(PSU)AGCAGA GCAGCUCCCU CGCUGCG AU CUAUUGAAAG UCAGCCCUCG ACACAAGGGU UUGU

GENBANK: GENBANK: GBCN01009604.1

Macromolecule #29: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 38.346707 KDa

Sequence

String:

UCUACGGCCA UACCACCCUG AACGCGCCCG AUCUCGUCUG AUCUCGGAAG CUAAGCAGGG UCGGGCCUGG UUAGUACUUG GAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU

GENBANK: GENBANK: GBCM01014045.1

Macromolecule #34: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 34 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 50.809047 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUUG

GENBANK: GENBANK: GBCN01009604.1

Macromolecule #63: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 63 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 603.92825 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUG(PSU)C(PSU)CAAA GAUUAAGCCA UGCAUGUCUA AGUACGCA C GGCCGGUACA GUGAAACUGC GAA(PSU)GGCUC(A2M) UUAAA(PSU)CAG(PSU) UAUGGU(OMU)CC(PSU) U (OMU)GGUCGCU CGCUCCUCUC CUACUUGGAU AACUGUGGUA (A2M)UUCUAG(A2M)GC UAA(OMU)A(OMC)AUGC CG ACGGGCG CUGACCCCCU UCGCGGGGGG GA(PSU)GCGUGCA (PSU)UUAUCAGAU CAAAACCAAC CCGGUCAGCC UCCCC CCGG CCCCGGCCGG GGGGGUGGGC GCCGGCGGCU UUGGUGACUC UAGAUAACCU CGGGCCGAUC GCACGCCCUC CGUGGC GGC GACGACCCAU UCGAACGUC(OMU) GCCCUAUCAA CUUUCGAUGG UAGUCGCCGU GCCUACCAUG GUGACCACGG G (PSU)GACGGGG AAUCAGGGUU CGAU(OMU)CCGGA GA(OMG)GGAGCCU GAGAAACGGC UACCACAU(OMC)C AAGG (A2M)AGGC AGCAGGCGCG C(A2M)AAUUACCC ACUCCCGACC CGGGGA(OMG)GU(A2M) GUGA(OMC)GAAAA AUAAC AAUA CAGGACUCUU UCGAGGCCCU GUAAUUGGAA UGAGUCCACU (PSU)UAA(A2M)UCCUU UAACGAGG(A2M)U CCAU UGGAG (OMG)GCAAGUC(PSU)G GUGCCAGCAG CCGCGG(OMU)AAU UCCAGCUCCA AUA(OMG)CGUA(PSU)A (PSU) UAAAGUUG CUGCAGUU(A2M)A AAAGCUCGUA G(PSU)U(OMG)GA(PSU)CUU GGGAGCGGGC GGGCGGUCCG CCGCGA GGC GAGCCACCGC CCGUCCCCGC CCCUUGCCUC UCGGCGCCCC CUCGAUGCUC UUAGCUGAGU GUCCCGCGGG GCCCGAA GC GUU(PSU)ACUUUG AAAAAA(PSU)(PSU)AG AGUG(PSU)UCAAA GCAGGCCCGA GCCGCCUGGA UACCGCAGCU AG GAA(PSU)AA(PSU) (OMG)GAAUAGGAC CGCGGUUCUA UUUUGUUGGU UUUCGGAACU GAGGCCAUGA UUAAGAGGGA C GGCCGGGG GCAUUCGUAU UGCGCCGCUA GAGGUGAAAU (PSU)CUUGGACCG GCGCAAGACG GACCAGAGCG AAAGCAUU (PSU) GCCAAGAAUG UUUUCAUUAA UCAAGA(A2M)CGA AAGUCGGAGG (PSU)(PSU)CGAAGACG A(PSU)CAGAUAC CGUCGUAGUU CCGACCA(PSU)AA ACGAUGCCGA CUGGCGAUGC GGCGGCGUUA UUCCCAUGAC CCGCCGGGCA GCUUCC GGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU A(PSU)GG(PSU)UGCAA AGCUGAAACU UAAAGGAAUU GACGGAAGG GCACCACCAG GAGUGGAGCC (PSU)GCGGC(PSU)UAA UU(PSU)GAC(B8N)CAA CACGGGAAAC CUCACCCGGC CCGG ACACG GACAGGA(OMU)UG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGG(OMU)G(OMG)UG CAUGGC(4AC)GU UCUUAGU(PSU)GG UGGAGCGAUU UGUCUGG(PSU)UA AUUCCGAUAA CGA(A2M)CGAGAC U(OMC)UGGCAUGC UAAC UAGUU ACGCGACCCC CGAGCGGUCG GCGUCCCCCA ACU(OMU)CU(PSU)A(OMG)A GGGACAAGUG GCGUUCAGCC ACC CGAGAU UGAGCAAUAA CA(OMG)GUCUGUG AUGCCCUUAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAU (PSU) CCCCAUGAAC GAG(G7M)AAU(PSU)CC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UU(A2M)AGUCCCU GCC CUU(PSU)GU ACACACCG(OMC)C CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCCG CCGGGG UCG GCCCACGGCC CUGGCGGAGC GCUGAGAAGA CGGUCGAACU (OMU)GACUAUCUA GAGGAAGUAA AAGUCGUA(6MZ) CAAGGUUUC(4AC) GUAGGUG(MA6)(MA6)C CUGCGGAAGG AUCAUUA

GENBANK: GENBANK: GBCT01000564.1

Macromolecule #87: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 87 ; Details: mRNA triplet complementary to the anticodon loop of the P-site tRNA. The mRNA nucleotides were modeled as N residues.; Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 543.327 Da

Sequence

String:

(N)(N)(N)

Macromolecule #90: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 90 / Number of copies: 420 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #91: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 91 / Number of copies: 8 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #92: UNKNOWN ATOM OR ION

• Macromolecule: Name: UNKNOWN ATOM OR ION / type: ligand / ID: 92 / Number of copies: 306 / Formula: UNX
• Molecular weight: Theoretical: 145.246 Da

Macromolecule #93: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 93 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #94: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 94 / Number of copies: 30 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #95: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 95 / Number of copies: 3 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #96: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 96 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #97: water

• Macromolecule: Name: water / type: ligand / ID: 97 / Number of copies: 2116 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.6 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.01 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 37182
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD