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基本情報
登録情報 | ![]() | ||||||||||||||||||
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タイトル | Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E | ||||||||||||||||||
![]() | homogeneously refined cryo-EM map of the quaternary RNC-NAC-NatA/E-MetAP1 complex | ||||||||||||||||||
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![]() | translation / ribosome / NAC / N-terminal acetyltransferase / NatA / NatE / MetAP1 | ||||||||||||||||||
機能・相同性 | ![]() negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / regulation of skeletal muscle fiber development / protein-N-terminal-glutamate acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / N-terminal protein amino acid acetylation / NatA complex / protein N-terminal-serine acetyltransferase activity / protein-N-terminal-alanine acetyltransferase activity / protein N-terminal-methionine acetyltransferase activity / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / protein-N-terminal amino-acid acetyltransferase activity / internal protein amino acid acetylation / N-terminal protein amino acid modification / peptidyl-methionine modification / acetyltransferase activator activity / N-acetyltransferase activity / histone H4 acetyltransferase activity / methionyl aminopeptidase / initiator methionyl aminopeptidase activity / heart trabecula morphogenesis / establishment of mitotic sister chromatid cohesion / skeletal muscle tissue regeneration / metalloexopeptidase activity / ribosomal subunit / exit from mitosis / optic nerve development / protein-lysine-acetyltransferase activity / mitotic sister chromatid cohesion / laminin receptor activity / retinal ganglion cell axon guidance / protein acetylation / metalloaminopeptidase activity / glutathione transferase / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / glutathione transferase activity / phagocytic cup / 90S preribosome / chromosome organization / aminopeptidase activity / protein-RNA complex assembly / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / laminin binding / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / glutathione metabolic process / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / protein maturation / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / wound healing / spindle / platelet aggregation / mRNA 5'-UTR binding / cytoplasmic ribonucleoprotein granule / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / protein transport / regulation of translation / large ribosomal subunit / heparin binding / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / angiogenesis / killing of cells of another organism / perikaryon / transcription regulator complex / cytosolic large ribosomal subunit / in utero embryonic development / cytoplasmic translation 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
![]() | Yudin D / Scaiola A / Ban N | ||||||||||||||||||
資金援助 | ![]() ![]() ![]()
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![]() | ![]() タイトル: NAC guides a ribosomal multienzyme complex for nascent protein processing. 著者: Alfred M Lentzsch / Denis Yudin / Martin Gamerdinger / Sowmya Chandrasekar / Laurenz Rabl / Alain Scaiola / Elke Deuerling / Nenad Ban / Shu-Ou Shan / ![]() ![]() ![]() 要旨: Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), ...Approximately 40% of the mammalian proteome undergoes N-terminal methionine excision and acetylation, mediated sequentially by methionine aminopeptidase (MetAP) and N-acetyltransferase A (NatA), respectively. Both modifications are strictly cotranslational and essential in higher eukaryotic organisms. The interaction, activity and regulation of these enzymes on translating ribosomes are poorly understood. Here we perform biochemical, structural and in vivo studies to demonstrate that the nascent polypeptide-associated complex (NAC) orchestrates the action of these enzymes. NAC assembles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the active sites of both enzymes for timely sequential processing of the nascent protein. NAC further releases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid protein K (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-terminal acetylation. Our results provide a mechanistic model for the cotranslational processing of proteins in eukaryotic cells. | ||||||||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 336.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 137 KB 137 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 21 KB | 表示 | ![]() |
画像 | ![]() | 138.9 KB | ||
マスクデータ | ![]() ![]() | 669.9 MB 669.9 MB | ![]() | |
Filedesc metadata | ![]() | 25.2 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() | 626.8 MB 625.5 MB 34 MB 621.6 MB 621.6 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9f1cMC ![]() 9f1bC ![]() 9f1dC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | homogeneously refined cryo-EM map of the quaternary RNC-NAC-NatA/E-MetAP1 complex | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.065 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
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投影像・断面図 |
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密度ヒストグラム |
-マスク #2
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to 6 A resolution
ファイル | emd_50125_additional_1.map | ||||||||||||
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注釈 | main map lowpass filtered to 6 A resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to 8 A resolution
ファイル | emd_50125_additional_2.map | ||||||||||||
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注釈 | main map lowpass filtered to 8 A resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to estimated local resolution
ファイル | emd_50125_additional_3.map | ||||||||||||
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注釈 | main map lowpass filtered to estimated local resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: halfmap A
ファイル | emd_50125_half_map_1.map | ||||||||||||
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注釈 | halfmap A | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: halfmap B
ファイル | emd_50125_half_map_2.map | ||||||||||||
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注釈 | halfmap B | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Quaternary complex of a translating 80S ribosome, NAC, MetAP1 and...
+超分子 #1: Quaternary complex of a translating 80S ribosome, NAC, MetAP1 and...
+超分子 #2: Rabbit ribosome-nascent chain complex
+超分子 #3: NAC heterodimer
+超分子 #4: Human methionine aminopeptidase 1
+超分子 #5: Human NatA/E complex
+超分子 #6: Human NatA complex
+超分子 #7: Human NatE with a GST tag
+分子 #1: mRNA
+分子 #3: 5S rRNA
+分子 #4: P-site tRNA
+分子 #6: 5.8S rRNA
+分子 #20: 28S rRNA
+分子 #64: 18S rRNA
+分子 #2: 60S ribosomal protein L29
+分子 #5: Small ribosomal subunit protein uS13
+分子 #7: 60S ribosomal protein L22
+分子 #8: 40S ribosomal protein S19
+分子 #9: Large ribosomal subunit protein uL2
+分子 #10: Ribosomal protein L23
+分子 #11: Small ribosomal subunit protein uS10
+分子 #12: Ribosomal protein L3
+分子 #13: Ribosomal_L23eN domain-containing protein
+分子 #14: Small ribosomal subunit protein eS21
+分子 #15: Large ribosomal subunit protein uL4
+分子 #16: Large ribosomal subunit protein uL22
+分子 #17: 60S ribosomal protein L27
+分子 #18: 40S ribosomal protein S23
+分子 #19: 60S ribosomal protein L6
+分子 #21: Large ribosomal subunit protein eL18
+分子 #22: 60S ribosomal protein L27a
+分子 #23: 40S ribosomal protein S24
+分子 #24: Ribosomal Protein uL30
+分子 #25: Ribosomal protein L26
+分子 #26: Ribosomal protein L24
+分子 #27: Small ribosomal subunit protein uS2
+分子 #28: 40S ribosomal protein S25
+分子 #29: 60S ribosomal protein L7a
+分子 #30: Ribosomal protein S15a
+分子 #31: Ribosomal protein L19
+分子 #32: 40S ribosomal protein S3a
+分子 #33: 60S ribosomal protein L41
+分子 #34: 60S ribosomal protein L9
+分子 #35: Large ribosomal subunit protein eL20
+分子 #36: Small ribosomal subunit protein uS5
+分子 #37: 60S ribosomal protein L30
+分子 #38: 60S ribosomal protein L10
+分子 #39: Large ribosomal subunit protein uL18
+分子 #40: Methionine aminopeptidase 1
+分子 #41: 40S ribosomal protein S3
+分子 #42: 60S ribosomal protein L31
+分子 #43: 60S ribosomal protein L11
+分子 #44: Nascent polypeptide-associated complex subunit alpha
+分子 #45: 40S ribosomal protein S4
+分子 #46: Ribosomal protein L32
+分子 #47: Nascent chain
+分子 #48: Transcription factor BTF3
+分子 #49: Ribosomal protein S5
+分子 #50: 60S ribosomal protein L35a
+分子 #51: Large ribosomal subunit protein eL13
+分子 #52: Glutathione S-transferase class-mu 26 kDa isozyme,N-alpha-acetylt...
+分子 #53: 40S ribosomal protein S6
+分子 #54: 60S ribosomal protein L34
+分子 #55: 60S ribosomal protein L14
+分子 #56: N-alpha-acetyltransferase 15, NatA auxiliary subunit
+分子 #57: 40S ribosomal protein S7
+分子 #58: 60S ribosomal protein L35
+分子 #59: Ribosomal protein L15
+分子 #60: N-alpha-acetyltransferase 10
+分子 #61: 40S ribosomal protein S8
+分子 #62: 60S ribosomal protein L36
+分子 #63: Large ribosomal subunit protein uL13
+分子 #65: 40S ribosomal protein S9
+分子 #66: Ribosomal protein L37
+分子 #67: 40S ribosomal protein S27
+分子 #68: S10_plectin domain-containing protein
+分子 #69: 60S ribosomal protein L38
+分子 #70: 40S ribosomal protein S28
+分子 #71: 40S ribosomal protein S11
+分子 #72: 60S ribosomal protein L39-like
+分子 #73: Ribosomal protein S27a
+分子 #74: 40S ribosomal protein S12
+分子 #75: Large ribosomal subunit protein eL40
+分子 #76: 40S ribosomal protein S30
+分子 #77: 40S ribosomal protein S13
+分子 #78: Large ribosomal subunit protein eL42
+分子 #79: Small ribosomal subunit protein eS26
+分子 #80: Small ribosomal subunit protein uS11
+分子 #81: 60S ribosomal protein L37a
+分子 #82: Small ribosomal subunit protein RACK1
+分子 #83: 40S ribosomal protein uS19
+分子 #84: Large ribosomal subunit protein eL28
+分子 #85: 40S ribosomal protein S29
+分子 #86: Small ribosomal subunit protein uS9
+分子 #87: 60S acidic ribosomal protein P0
+分子 #88: 60S ribosomal protein L21
+分子 #89: 40S ribosomal protein eS17
+分子 #90: 60S ribosomal protein L12
+分子 #91: UNKNOWN ATOM OR ION
+分子 #92: MAGNESIUM ION
+分子 #93: GUANOSINE-5'-TRIPHOSPHATE
+分子 #94: SPERMIDINE
+分子 #95: SPERMINE
+分子 #96: ZINC ION
+分子 #97: INOSITOL HEXAKISPHOSPHATE
+分子 #98: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: OTHER / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.4 µm / 最小 デフォーカス(公称値): 0.6 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |