National Institutes of Health/National Institute on Deafness and Other Communication Disorders (NIH/NIDCD)
5R00DC019401
米国
Howard Hughes Medical Institute (HHMI)
米国
引用
ジャーナル: Science / 年: 2024 タイトル: Structural basis of odor sensing by insect heteromeric odorant receptors. 著者: Jiawei Zhao / Andy Q Chen / Jaewook Ryu / Josefina Del Mármol / 要旨: Most insects, including human-targeting mosquitoes, detect odors through odorant-activated ion channel complexes consisting of a divergent odorant-binding subunit (OR) and a conserved co-receptor ...Most insects, including human-targeting mosquitoes, detect odors through odorant-activated ion channel complexes consisting of a divergent odorant-binding subunit (OR) and a conserved co-receptor subunit (Orco). As a basis for understanding how odorants activate these heteromeric receptors, we report here cryo-electron microscopy structures of two different heteromeric odorant receptor complexes containing ORs from disease-vector mosquitos or . These structures reveal an unexpected stoichiometry of one OR to three Orco subunits. Comparison of structures in odorant-bound and unbound states indicates that odorant binding to the sole OR subunit is sufficient to open the channel pore, suggesting a mechanism of OR activation and a conceptual framework for understanding evolution of insect odorant receptor sensitivity.