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Yorodumi- PDB-8rqg: Histidine-containing phosphotransfer protein from Chaetomium term... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8rqg | ||||||
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Title | Histidine-containing phosphotransfer protein from Chaetomium termophilum mutant H105E | ||||||
Components | Histidine-containing phosphotransfer protein | ||||||
Keywords | TRANSFERASE / Histidine-containing transferase / fungal / Phosphorelay | ||||||
Function / homology | Function and homology information protein histidine kinase binding / histidine phosphotransfer kinase activity / phosphorelay signal transduction system / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Thermochaetoides thermophila (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Paredes-Martinez, F. / Casino, P. | ||||||
Funding support | Spain, 1items
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Citation | Journal: Commun Biol / Year: 2024 Title: Structural and functional insights underlying recognition of histidine phosphotransfer protein in fungal phosphorelay systems. Authors: Paredes-Martinez, F. / Eixeres, L. / Zamora-Caballero, S. / Casino, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8rqg.cif.gz | 40.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8rqg.ent.gz | 26 KB | Display | PDB format |
PDBx/mmJSON format | 8rqg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rq/8rqg ftp://data.pdbj.org/pub/pdb/validation_reports/rq/8rqg | HTTPS FTP |
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-Related structure data
Related structure data | 8pbwC 8pdcC 8phnC 8phxC 8rqjC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18913.711 Da / Num. of mol.: 1 / Mutation: H105E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermochaetoides thermophila (fungus) / Gene: CTHT_0013690 / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): Shuffle-T7 / References: UniProt: G0S1I2 | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.8 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop Details: 500 mM Ammonium dihydrogen Phosphate 200 mM Sodium Citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97918 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 18, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→87.1 Å / Num. obs: 8055 / % possible obs: 100 % / Redundancy: 51.2 % / CC1/2: 1 / Rmerge(I) obs: 0.2 / Rpim(I) all: 0.028 / Rrim(I) all: 0.203 / Χ2: 1 / Net I/σ(I): 27.5 |
Reflection shell | Resolution: 2.4→2.49 Å / Num. unique obs: 825 / CC1/2: 0.799 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→87.1 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.905 / SU B: 8.525 / SU ML: 0.192 / Cross valid method: THROUGHOUT / ESU R: 0.279 / ESU R Free: 0.237 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.868 Å2
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Refinement step | Cycle: 1 / Resolution: 2.4→87.1 Å
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