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- PDB-8phx: Receiver Domain of the Hybrid Histidine Kinase Sln1 of Candida al... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8phx | ||||||
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Title | Receiver Domain of the Hybrid Histidine Kinase Sln1 of Candida albicans | ||||||
![]() | Histidine protein kinase SLN1 | ||||||
![]() | SIGNALING PROTEIN / hybrid histidine kinase / Fungal / phosphorelay / kinase | ||||||
Function / homology | ![]() cellular response to farnesol / positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus / filamentous growth of a population of unicellular organisms in response to biotic stimulus / osmosensor activity / osmosensory signaling via phosphorelay pathway / protein histidine kinase activity / histidine phosphotransfer kinase activity / histidine kinase / phosphorelay sensor kinase activity / cell wall organization ...cellular response to farnesol / positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus / filamentous growth of a population of unicellular organisms in response to biotic stimulus / osmosensor activity / osmosensory signaling via phosphorelay pathway / protein histidine kinase activity / histidine phosphotransfer kinase activity / histidine kinase / phosphorelay sensor kinase activity / cell wall organization / ATP binding / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Paredes-Martinez, F. / Casino, P. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional insights underlying recognition of histidine phosphotransfer protein in fungal phosphorelay systems. Authors: Paredes-Martinez, F. / Eixeres, L. / Zamora-Caballero, S. / Casino, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.7 KB | Display | ![]() |
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PDB format | ![]() | 93.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.7 KB | Display | ![]() |
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Full document | ![]() | 451.2 KB | Display | |
Data in XML | ![]() | 25.9 KB | Display | |
Data in CIF | ![]() | 37.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8pbwC ![]() 8pdcC ![]() 8phnC ![]() 8rqgC ![]() 8rqjC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14676.970 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-MG / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.67 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 30 % w/v PEG 4000 0.1 M Tris-HCl pH 8.5 0.2 M Magnesium Chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 16, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→97.28 Å / Num. obs: 98531 / % possible obs: 100 % / Redundancy: 7.3 % / CC1/2: 1 / Rmerge(I) obs: 0.058 / Rpim(I) all: 0.023 / Rrim(I) all: 0.062 / Χ2: 1.01 / Net I/σ(I): 17.3 / Num. measured all: 723181 |
Reflection shell | Resolution: 1.5→1.53 Å / % possible obs: 100 % / Redundancy: 7.4 % / Rmerge(I) obs: 0.995 / Num. measured all: 35636 / Num. unique obs: 4803 / CC1/2: 0.749 / Rpim(I) all: 0.386 / Rrim(I) all: 1.069 / Χ2: 1 / Net I/σ(I) obs: 2.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.423 Å2
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Refinement step | Cycle: 1 / Resolution: 1.5→54.2 Å
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Refine LS restraints |
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