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- PDB-8pje: Human Leukocyte Antigen class II allotype DR1 presenting influenz... -

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Basic information

Entry
Database: PDB / ID: 8pje
TitleHuman Leukocyte Antigen class II allotype DR1 presenting influenza A virus haemagglutinin (HA)306-318 PKYVKQNTLKLAT
Components
  • (HLA class II histocompatibility antigen, ...) x 2
  • Hemagglutinin
KeywordsIMMUNE SYSTEM / HLA-II / HLA-DR / HLA-DR1 / human leukocyte antigen / major histocompatibility complex / major histocompatibility complex class 2 / influenza A virus / flu / haemagglutinin / HA / infection / vaccine
Function / homology
Function and homology information


regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II ...regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of T cell mediated immune response to tumor cell / positive regulation of kinase activity / positive regulation of memory T cell differentiation / positive regulation of monocyte differentiation / inflammatory response to antigenic stimulus / CD4 receptor binding / intermediate filament / T-helper 1 type immune response / transport vesicle membrane / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / polysaccharide binding / negative regulation of type II interferon production / humoral immune response / macrophage differentiation / Generation of second messenger molecules / immunological synapse / Co-inhibition by PD-1 / epidermis development / positive regulation of insulin secretion involved in cellular response to glucose stimulus / detection of bacterium / T cell receptor binding / negative regulation of T cell proliferation / MHC class II antigen presentation / viral budding from plasma membrane / trans-Golgi network membrane / protein tetramerization / peptide antigen assembly with MHC class II protein complex / lumenal side of endoplasmic reticulum membrane / negative regulation of inflammatory response to antigenic stimulus / MHC class II protein complex / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / positive regulation of T cell activation / peptide antigen binding / structural constituent of cytoskeleton / positive regulation of T cell mediated cytotoxicity / cognition / positive regulation of protein phosphorylation / Interferon gamma signaling / MHC class II protein complex binding / endocytic vesicle membrane / late endosome membrane / Downstream TCR signaling / T cell receptor signaling pathway / early endosome membrane / clathrin-dependent endocytosis of virus by host cell / adaptive immune response / positive regulation of viral entry into host cell / lysosome / positive regulation of ERK1 and ERK2 cascade / positive regulation of canonical NF-kappaB signal transduction / positive regulation of MAPK cascade / host cell surface receptor binding / immune response / Golgi membrane / fusion of virus membrane with host plasma membrane / lysosomal membrane / external side of plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / positive regulation of DNA-templated transcription / virion attachment to host cell / host cell plasma membrane / virion membrane / cell surface / signal transduction / extracellular space / extracellular exosome / identical protein binding / membrane / plasma membrane
Similarity search - Function
MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin ...MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
HLA class II histocompatibility antigen, DR alpha chain / HLA class II histocompatibility antigen, DRB1 beta chain / Hemagglutinin
Similarity search - Component
Biological speciesHomo sapiens (human)
Influenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å
AuthorsMacLachlan, B.J. / Wall, A. / Greenshields-Watson, A.L. / Hesketh, S.J. / Cole, D.K. / Rizkallah, P.J. / Godkin, A.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust209213/Z/17/Z United Kingdom
CitationJournal: Cell Rep / Year: 2024
Title: A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4 + T cell activation.
Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, ...Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, G.H. / Smart, K. / Scourfield, D.O. / Scott, J. / Sukhova, K. / Stanton, R.J. / Wall, A. / Rizkallah, P.J. / Barclay, W.S. / Gallimore, A. / Godkin, A.
History
DepositionJun 23, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 19, 2024Provider: repository / Type: Initial release
Revision 1.1Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen, DRB1 beta chain
C: Hemagglutinin
D: HLA class II histocompatibility antigen, DR alpha chain
E: HLA class II histocompatibility antigen, DRB1 beta chain
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)94,27846
Polymers91,1136
Non-polymers3,16540
Water10,287571
1
A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen, DRB1 beta chain
C: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,33825
Polymers45,5563
Non-polymers1,78222
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: HLA class II histocompatibility antigen, DR alpha chain
E: HLA class II histocompatibility antigen, DRB1 beta chain
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,94021
Polymers45,5563
Non-polymers1,38418
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)41.500, 89.180, 137.440
Angle α, β, γ (deg.)90.000, 90.900, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 4 through 18 or resid 20...
d_2ens_1(chain "D" and (resid 4 through 18 or resid 20...
d_1ens_2(chain "B" and (resid -2 through 34 or resid 36...
d_2ens_2(chain "E" and (resid -2 through 34 or resid 36...
d_1ens_3(chain "C" and (resid 1 through 9 or resid 11 through 13))
d_2ens_3(chain "F" and (resid 1 through 9 or resid 11 through 13))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1GLUGLNA2 - 16
d_12ens_1GLYPHEA18 - 20
d_13ens_1PHELEUA22 - 43
d_14ens_1GLULEUA45 - 68
d_15ens_1ILELYSA70 - 73
d_16ens_1SERLEUA75 - 97
d_17ens_1GLULEUA99 - 120
d_18ens_1ASNPHEA122 - 143
d_19ens_1LYSPROA145 - 153
d_110ens_1THRASPA155 - 179
d_21ens_1GLUGLND2 - 16
d_22ens_1GLYPHED18 - 20
d_23ens_1PHELEUD22 - 43
d_24ens_1GLULEUD45 - 68
d_25ens_1ILELYSD70 - 73
d_26ens_1SERLEUD75 - 97
d_27ens_1GLULEUD99 - 120
d_28ens_1ASNPHED122 - 143
d_29ens_1LYSPROD145 - 153
d_210ens_1THRASPD155 - 179
d_11ens_2GLYGLNB1 - 37
d_12ens_2GLUALAB39 - 77
d_13ens_2ASPLEUB79 - 112
d_14ens_2HISGLYB114 - 128
d_15ens_2ILEPROB130 - 168
d_16ens_2SERALAB170 - 193
d_21ens_2GLYGLNE2 - 38
d_22ens_2GLUALAE40 - 78
d_23ens_2ASPLEUE80 - 113
d_24ens_2HISGLYE115 - 129
d_25ens_2ILEPROE131 - 169
d_26ens_2SERALAE171 - 194
d_11ens_3PROLEUC1 - 9
d_12ens_3LEUTHRC11 - 13
d_21ens_3PROLEUF1 - 9
d_22ens_3LEUTHRF11 - 13

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(0.999999071285, 0.00134061954109, 0.000245293668599), (0.00134493038461, -0.999827762082, -0.0185104654768), (0.000220435927996, 0.0185107781887, -0.999828636567)-1.01540150521, -1.31613479791, -68.714335242
2given(0.999991811189, 0.00210878331496, 0.00345406830302), (0.00215376792429, -0.999912236435, -0.0130721349174), (0.00342619886166, 0.0130794671336, -0.999908590173)-0.821113379329, -1.10405958026, -68.6602497384
3given(0.999933147196, 0.00849877621049, -0.00784040448567), (0.00834704167884, -0.99978107363, -0.0191867586466), (-0.00800175198232, 0.019120031775, -0.9997851751)-1.55795844237, -1.40377260236, -68.6871232628

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Components

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HLA class II histocompatibility antigen, ... , 2 types, 4 molecules ADBE

#1: Protein HLA class II histocompatibility antigen, DR alpha chain / MHC class II antigen DRA


Mass: 21562.426 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRA, HLA-DRA1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01903
#2: Protein HLA class II histocompatibility antigen, DRB1 beta chain / Human leukocyte antigen DRB1 / HLA-DRB1


Mass: 22487.186 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRB1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01911

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Protein/peptide , 1 types, 2 molecules CF

#3: Protein/peptide Hemagglutinin


Mass: 1506.807 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Influenza A virus / References: UniProt: P03435

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Non-polymers , 4 types, 611 molecules

#4: Chemical ChemComp-EPE / 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES


Mass: 238.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H18N2O4S / Comment: pH buffer*YM
#5: Chemical...
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 27 / Source method: obtained synthetically / Formula: C2H6O2
#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: C3H8O3
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 571 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.54 Å3/Da / Density % sol: 51.6 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 0.1 M HEPES pH 7.0, 25 % PEG8000, 15 % Glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97625 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jul 13, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97625 Å / Relative weight: 1
ReflectionResolution: 1.7→32.81 Å / Num. obs: 109380 / % possible obs: 99.45 % / Redundancy: 3.7 % / Biso Wilson estimate: 23.46 Å2 / CC1/2: 0.997 / CC star: 0.999 / Rmerge(I) obs: 0.0833 / Rpim(I) all: 0.049 / Rrim(I) all: 0.097 / Net I/σ(I): 9.48
Reflection shellResolution: 1.7→1.76 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.884 / Mean I/σ(I) obs: 1.36 / Num. unique obs: 10898 / CC1/2: 0.545 / CC star: 0.84 / Rpim(I) all: 0.539 / Rrim(I) all: 1.039 / % possible all: 99.55

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
xia2data reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.7→32.81 Å / SU ML: 0.2137 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.4456
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2026 5555 5.08 %
Rwork0.1708 103810 -
obs0.1724 109365 99.47 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 31.64 Å2
Refinement stepCycle: LAST / Resolution: 1.7→32.81 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6316 0 204 571 7091
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01876712
X-RAY DIFFRACTIONf_angle_d1.63879060
X-RAY DIFFRACTIONf_chiral_restr0.1129956
X-RAY DIFFRACTIONf_plane_restr0.01391166
X-RAY DIFFRACTIONf_dihedral_angle_d9.3188937
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.457217567336
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.593800169687
ens_3d_2CX-RAY DIFFRACTIONTorsion NCS0.247503677638
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.7-1.720.31841860.28033510X-RAY DIFFRACTION99.57
1.72-1.740.31441820.27213440X-RAY DIFFRACTION99.51
1.74-1.760.32321850.26173394X-RAY DIFFRACTION99.58
1.76-1.780.32241900.25313468X-RAY DIFFRACTION99.67
1.78-1.810.25611960.24363468X-RAY DIFFRACTION99.59
1.81-1.830.26651650.24173426X-RAY DIFFRACTION99.58
1.83-1.860.35061690.29843472X-RAY DIFFRACTION99.56
1.86-1.890.33121880.28913497X-RAY DIFFRACTION99.54
1.89-1.910.29441660.2623387X-RAY DIFFRACTION99.64
1.91-1.950.25421700.2213496X-RAY DIFFRACTION99.59
1.95-1.980.26551950.20273471X-RAY DIFFRACTION99.46
1.98-2.020.21191850.18113394X-RAY DIFFRACTION99.61
2.02-2.050.21971730.18633493X-RAY DIFFRACTION99.59
2.05-2.10.2251810.1873494X-RAY DIFFRACTION99.78
2.1-2.140.22821780.19063425X-RAY DIFFRACTION99.7
2.14-2.190.21971840.18793505X-RAY DIFFRACTION99.7
2.19-2.250.21931770.1783440X-RAY DIFFRACTION99.83
2.25-2.310.22291630.17553524X-RAY DIFFRACTION99.76
2.31-2.370.22411970.17783409X-RAY DIFFRACTION99.75
2.38-2.450.23651820.17333502X-RAY DIFFRACTION99.89
2.45-2.540.19972130.16893413X-RAY DIFFRACTION99.64
2.54-2.640.19712090.16533469X-RAY DIFFRACTION99.81
2.64-2.760.20481960.16733487X-RAY DIFFRACTION99.78
2.76-2.910.16982080.16183415X-RAY DIFFRACTION99.64
2.91-3.090.20011940.16743449X-RAY DIFFRACTION99.24
3.09-3.330.17381530.16313505X-RAY DIFFRACTION99.46
3.33-3.660.17731830.14663461X-RAY DIFFRACTION98.91
3.66-4.190.17882030.14023449X-RAY DIFFRACTION98.41
4.19-5.270.1441820.11823478X-RAY DIFFRACTION98.71
5.28-32.810.17362020.15323469X-RAY DIFFRACTION97.61
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.15179149271-3.026789359695.018926371693.61627691126-3.824539381385.305438252210.3253066293750.54507600128-0.0127877146876-0.405843314837-0.402300245963-0.2204385644990.4061473001941.012295284210.1908626915740.2289211407450.0596815010510.05169323692490.2277408925780.01954308078990.2433996125516.25814314209-17.3390220828-20.7869721019
22.76990171412.54561290972-1.101917021374.62752673511-0.9937291925833.352385187940.0764409117955-0.02417042906520.137100009597-0.0519826659653-0.05029691209390.0760339456038-0.213909820501-0.05280444796350.0003683271949980.08626840673190.00410598109996-0.004083569422370.132328212494-0.002469206789170.137371276653-2.9234838401714.1773327251-53.8806853992
35.90839665402-0.5404248698831.174143026486.05760578645-2.430857007495.915195941310.008795753813540.249002662697-0.133434166997-0.3936688638620.0267228707848-0.1150634836850.1252193158760.215782177862-0.001125660835060.13322744502-0.00206303126260.03533632097870.118677201571-0.01801659755330.1260899963763.5219359154212.9574018028-64.3659645656
41.218020092790.2206781416910.584159061691.24050649493-0.1319097492031.276291494230.0511711676360.0281405996709-0.00407201483414-0.137523873855-0.05757803468050.04800153434130.101029156079-0.0186431698795-0.01707432217740.1355394896310.00545870148170.006336516494190.120353663538-0.01905187224960.153850626358-11.93770870326.19623668708-58.8680729835
52.657244845931.020937727091.905572411653.379013714763.029063676183.13150641584-0.05229706950310.04177777836430.127917285264-0.2347269103420.05494919138160.0338988196984-0.2648753878040.237297944076-0.005171619222040.1748946539530.012128260189-0.002524175551820.1398036552640.01106609301430.176209153462-12.058167978310.8686013147-61.7500491536
62.134658213360.1868157252031.710433634152.05394763335-0.5242518664926.8946610861-0.005414193486290.2211070661930.0153043496974-0.4916902982580.03910791674920.4354771817110.407307041279-0.515164005093-0.03778851529960.225849489892-0.0257995092447-0.09651117170120.224718855057-0.01079550870090.254495694789-23.85707561462.08806899708-68.1636295514
71.240975167390.804936016146-0.3590747284361.7958425822-0.149376404161.378646287720.280107088653-0.2873654916450.2957861536930.273067979108-0.2599053743690.0500833551123-0.3484124955040.141937685985-0.00765191814320.222996374792-0.05107493453970.01909066765220.183081343382-0.04079209869210.23155644097-1.2356661480721.6323401654-45.3529821958
84.93606259116-1.272983145981.483103862312.20134127143-1.056176086192.04476519314-0.005769432785340.117430235934-0.0318845816541-0.06109127313090.04545361094110.1837061858410.0428058325763-0.162181825817-0.02639080646880.1908290824720.0138342525855-0.004843619872690.1535190621610.004098080812440.155340498512-23.164806908427.4768933838-78.4810236029
95.884062913944.36100384587-5.155464404656.25205092532-5.119436515315.074842055220.268462133198-0.645439893456-0.1146650424410.40180571764-0.571563452568-0.339135243068-0.2404037921771.149246642840.394223879480.160059409086-0.033343982043-0.02160208262090.208865305177-0.01701117216560.2468130197347.2994224326416.9084867486-47.6222620661
102.3330061542-2.519302709990.9538512989754.81132218235-2.139467366013.913734197660.101938777435-0.03248503558-0.156757775151-0.115599692158-0.08237451009980.07175249244770.229672669238-0.03482398662620.01605049924560.113458649295-0.009660688013890.01085059818510.1283002031980.007770995531860.143295014262-3.86002382943-14.4706272519-14.6095238853
116.354909045580.898727436574-1.138274105746.25357680795-1.292053931045.634472610430.0343413588362-0.2912240273930.1623981787870.308763087511-0.0278786026036-0.205037034083-0.1051228460550.211281628397-0.009087440098930.148146121425-0.00432520278526-0.02386816131780.121169932146-0.005630141830580.1299784715182.53129488177-13.0900922728-4.12283246015
124.67074599527-1.524344992786.028897288441.96729069464-1.527718099237.913533972760.07600016535230.3063357208110.18045494438-0.362534086456-0.304072779056-0.252302752510.2174850186060.2474537615630.2137751876770.2067018101950.04977442915630.07838835133450.2398918337890.04017683661080.2054211556641.13714940182-8.2183380505-25.675940482
131.30420347697-0.30411459892-0.9872699084871.617427656520.1061782419664.188402026810.0454419036274-0.1274816555840.005546178965860.261569131093-0.008737182970420.166872964031-0.104773199960.0376736866201-0.01464129335410.155599601718-0.01036875900840.02139282701150.12804195434-0.01842089419310.168640256752-16.9074575228-7.22456891428-5.74015466068
141.29727265372-0.443542449811-0.7402825717941.668456715770.1703875379066.46065486613-0.0170344371694-0.1513080585270.05614372682060.5102128947750.02633935553510.462307280633-0.357632488982-0.515682196859-0.02394478606670.267472314004-0.01187816087010.1098104785220.18686062756-0.0181867912010.253057547028-21.8187817169-3.8493126863-0.667209035503
150.84175670241-0.359967460210.3631849190342.29695478823-0.2396645982971.65868575020.2666115094620.178338749652-0.199630695499-0.427102209737-0.217593489545-0.03706320702140.3660294533370.161465847666-0.0439098781370.2758884771770.06145927802880.01213938378230.186203952669-0.004960406572840.200761835268-2.47057387246-21.8104741295-22.9572449437
164.175309663881.34032597747-1.431007494211.52047451872-0.8054796192682.10677816119-0.00121502439612-0.08180792137970.095655058428-0.01909225378760.01712324414380.1456308958810.0325831456665-0.165635570505-0.008427103726710.224977078231-0.01971828309710.02307971302420.1914549249880.009035234075910.189925282994-24.2443022154-27.591625556510.1303291064
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'F' and (resid 1 through 13 )FF1 - 131 - 13
22chain 'A' and (resid 3 through 26 )AA3 - 261 - 24
33chain 'A' and (resid 27 through 55 )AA27 - 5525 - 53
44chain 'A' and (resid 56 through 133 )AA56 - 13354 - 131
55chain 'A' and (resid 134 through 154 )AA134 - 154132 - 152
66chain 'A' and (resid 155 through 181 )AA155 - 181153 - 179
77chain 'B' and (resid -2 through 89 )BB-2 - 891 - 92
88chain 'B' and (resid 90 through 190 )BB90 - 19093 - 193
99chain 'C' and (resid 1 through 13 )CC1 - 131 - 13
1010chain 'D' and (resid 3 through 26 )DD3 - 261 - 24
1111chain 'D' and (resid 27 through 55 )DD27 - 5525 - 53
1212chain 'D' and (resid 56 through 76 )DD56 - 7654 - 74
1313chain 'D' and (resid 77 through 144 )DD77 - 14475 - 142
1414chain 'D' and (resid 145 through 181 )DD145 - 181143 - 179
1515chain 'E' and (resid -3 through 89 )EE-3 - 891 - 93
1616chain 'E' and (resid 90 through 190 )EE90 - 19094 - 194

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