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- PDB-8pjg: F11 TCR in complex with Human Leukocyte Antigen class II allotype... -

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Basic information

Entry
Database: PDB / ID: 8pjg
TitleF11 TCR in complex with Human Leukocyte Antigen class II allotype DR1 presenting P11T->R modified influenza A virus haemagglutinin (HA)306-318 PKYVKQNTLKLAR
Components
  • (HLA class II histocompatibility antigen, ...) x 2
  • (T cell receptor ...) x 2
  • Hemagglutinin HA2 chain
KeywordsIMMUNE SYSTEM / HLA-II / HLA-DR / HLA-DR1 / human leukocyte antigen / major histocompatibility complex / major histocompatibility complex class 2 / influenza A virus / flu / haemagglutinin / HA / infection / vaccine
Function / homology
Function and homology information


regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / positive regulation of T cell mediated immune response to tumor cell / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation ...regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / positive regulation of T cell mediated immune response to tumor cell / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of memory T cell differentiation / positive regulation of monocyte differentiation / CD4 receptor binding / inflammatory response to antigenic stimulus / positive regulation of kinase activity / transport vesicle membrane / intermediate filament / polysaccharide binding / T-helper 1 type immune response / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / positive regulation of insulin secretion involved in cellular response to glucose stimulus / humoral immune response / macrophage differentiation / negative regulation of type II interferon production / Generation of second messenger molecules / immunological synapse / PD-1 signaling / epidermis development / T cell receptor binding / detection of bacterium / negative regulation of T cell proliferation / negative regulation of inflammatory response to antigenic stimulus / MHC class II antigen presentation / viral budding from plasma membrane / trans-Golgi network membrane / lumenal side of endoplasmic reticulum membrane / protein tetramerization / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / structural constituent of cytoskeleton / cognition / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / positive regulation of T cell mediated cytotoxicity / peptide antigen binding / antigen processing and presentation of exogenous peptide antigen via MHC class II / endocytic vesicle membrane / Interferon gamma signaling / positive regulation of immune response / Downstream TCR signaling / positive regulation of T cell activation / MHC class II protein complex binding / late endosome membrane / T cell receptor signaling pathway / early endosome membrane / clathrin-dependent endocytosis of virus by host cell / positive regulation of canonical NF-kappaB signal transduction / adaptive immune response / positive regulation of MAPK cascade / positive regulation of viral entry into host cell / positive regulation of ERK1 and ERK2 cascade / lysosome / host cell surface receptor binding / positive regulation of protein phosphorylation / immune response / lysosomal membrane / fusion of virus membrane with host plasma membrane / external side of plasma membrane / Golgi membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / positive regulation of DNA-templated transcription / cell surface / signal transduction / extracellular space / extracellular exosome / identical protein binding / membrane / plasma membrane
Similarity search - Function
MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin ...MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE / HLA class II histocompatibility antigen, DR alpha chain / HLA class II histocompatibility antigen, DRB1 beta chain / Hemagglutinin
Similarity search - Component
Biological speciesHomo sapiens (human)
Influenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.83 Å
AuthorsMacLachlan, B.J. / Wall, A. / Greenshields-Watson, A.L. / Cole, D.K. / Rizkallah, P.J. / Godkin, A.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust209213/Z/17/Z United Kingdom
CitationJournal: Cell Rep / Year: 2024
Title: A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4 + T cell activation.
Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, ...Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, G.H. / Smart, K. / Scourfield, D.O. / Scott, J. / Sukhova, K. / Stanton, R.J. / Wall, A. / Rizkallah, P.J. / Barclay, W.S. / Gallimore, A. / Godkin, A.
History
DepositionJun 23, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 19, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen, DRB1 beta chain
C: Hemagglutinin HA2 chain
D: T cell receptor alpha chain constant
E: T cell receptor beta constant 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)96,61022
Polymers95,3555
Non-polymers1,25517
Water7,422412
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: homology, surface plasmon resonance
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17440 Å2
ΔGint-6 kcal/mol
Surface area36620 Å2
Unit cell
Length a, b, c (Å)132.610, 184.820, 50.120
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x+1/2,y+1/2,-z
#4: -x,-y,z

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Components

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HLA class II histocompatibility antigen, ... , 2 types, 2 molecules AB

#1: Protein HLA class II histocompatibility antigen, DR alpha chain / MHC class II antigen DRA


Mass: 21287.102 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRA, HLA-DRA1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01903
#2: Protein HLA class II histocompatibility antigen, DRB1 beta chain / Human leukocyte antigen DRB1 / HLA-DRB1


Mass: 22211.859 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRB1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01911

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Protein/peptide , 1 types, 1 molecules C

#3: Protein/peptide Hemagglutinin HA2 chain


Mass: 1562.896 Da / Num. of mol.: 1 / Mutation: T318R / Source method: obtained synthetically / Source: (synth.) Influenza A virus / References: UniProt: P03435

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T cell receptor ... , 2 types, 2 molecules DE

#4: Protein T cell receptor alpha chain constant


Mass: 22969.627 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAC, TCRA / Production host: Escherichia coli BL21(DE3) (bacteria)
#5: Protein T cell receptor beta constant 1


Mass: 27323.443 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBC1 / Production host: Escherichia coli BL21(DE3) (bacteria)

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Non-polymers , 3 types, 429 molecules

#6: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: C2H6O2
#7: Chemical ChemComp-P4G / 1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE


Mass: 162.227 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H18O3
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 412 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.29 Å3/Da / Density % sol: 62.7 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 0.1 M Sodium cacodylate pH 6.5, 15 % PEG8000, 0.2 M ammonium sulphate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97623 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jul 23, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97623 Å / Relative weight: 1
ReflectionResolution: 1.83→66.31 Å / Num. obs: 108094 / % possible obs: 98.57 % / Redundancy: 7.4 % / Biso Wilson estimate: 32.03 Å2 / CC1/2: 0.998 / CC star: 1 / Rmerge(I) obs: 0.0916 / Rpim(I) all: 0.0363 / Rrim(I) all: 0.0987 / Net I/σ(I): 12.76
Reflection shellResolution: 1.83→1.9 Å / Redundancy: 7.5 % / Rmerge(I) obs: 1.635 / Mean I/σ(I) obs: 1.43 / Num. unique obs: 10599 / CC1/2: 0.54 / CC star: 0.838 / Rpim(I) all: 0.636 / Rrim(I) all: 1.756 / % possible all: 97.95

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
xia2data reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.83→66.31 Å / SU ML: 0.2298 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.7264
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2138 5364 4.96 %
Rwork0.1811 102722 -
obs0.1827 108086 98.57 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 51.84 Å2
Refinement stepCycle: LAST / Resolution: 1.83→66.31 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6640 0 82 412 7134
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01577001
X-RAY DIFFRACTIONf_angle_d1.45599502
X-RAY DIFFRACTIONf_chiral_restr0.10011020
X-RAY DIFFRACTIONf_plane_restr0.01791242
X-RAY DIFFRACTIONf_dihedral_angle_d19.1116971
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.83-1.850.42451690.35393309X-RAY DIFFRACTION96.24
1.85-1.870.36891670.32633379X-RAY DIFFRACTION98.86
1.87-1.90.28981750.30363398X-RAY DIFFRACTION98.78
1.9-1.920.33421790.33288X-RAY DIFFRACTION95.88
1.92-1.940.28431970.27053359X-RAY DIFFRACTION98.5
1.94-1.970.28721700.24123406X-RAY DIFFRACTION99.36
1.97-20.2721770.21793294X-RAY DIFFRACTION95.96
2-2.030.23211750.21583400X-RAY DIFFRACTION99.14
2.03-2.060.23792010.20643400X-RAY DIFFRACTION99.28
2.06-2.090.24951620.20693313X-RAY DIFFRACTION96.34
2.09-2.130.21231860.19873417X-RAY DIFFRACTION99.97
2.13-2.170.23831830.19873382X-RAY DIFFRACTION97.51
2.17-2.210.21471750.1943395X-RAY DIFFRACTION98.76
2.21-2.260.20651850.19163402X-RAY DIFFRACTION99.23
2.26-2.310.2181950.19143367X-RAY DIFFRACTION97.78
2.31-2.360.19981690.17743442X-RAY DIFFRACTION99.78
2.36-2.420.2181800.17723370X-RAY DIFFRACTION97.8
2.42-2.480.2141600.18313482X-RAY DIFFRACTION99.78
2.48-2.560.211580.18113395X-RAY DIFFRACTION98.12
2.56-2.640.21211950.18483445X-RAY DIFFRACTION98.48
2.64-2.730.22931730.18513437X-RAY DIFFRACTION100
2.73-2.840.20981650.19243453X-RAY DIFFRACTION98.85
2.84-2.970.21321840.193458X-RAY DIFFRACTION99.05
2.97-3.130.22681890.18473452X-RAY DIFFRACTION98.83
3.13-3.330.22131920.17223477X-RAY DIFFRACTION99.7
3.33-3.580.20071720.17153491X-RAY DIFFRACTION99.03
3.58-3.940.21241680.16593469X-RAY DIFFRACTION98.32
3.94-4.510.20851750.14523554X-RAY DIFFRACTION99.71
4.51-5.680.15991810.14973575X-RAY DIFFRACTION99.21
5.69-66.310.19742070.17983713X-RAY DIFFRACTION98.79
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.9849496069570.615072582643-0.364461538424-0.8496508503390.0586772204285-0.08050914682580.410453600910.939114547999-0.4531745692910.158985789248-0.284928608378-0.0767355971735-0.045637486492-0.7087661385540.02287633617370.3916575121910.108444038278-0.002890945603971.25757229178-0.2312683505851.18727704073-79.874562220221.9114779353-25.6428890093
21.377923602730.55726937621-0.4891340223435.7214391398-2.061025981832.8007038850.101107734302-0.00804324806781-0.33355458499-0.250390898267-0.3868242683130.0795001365035-0.197420173197-0.2464897347420.1885763383320.3591836968890.179567635447-0.07892292508380.458706440399-0.07908377771530.53667009628-38.974330152931.9604817302-27.8314278006
33.08243278935-2.125578898680.13153410091.903244432420.5379027922991.136846893440.02273251184630.0948968634935-0.122346248846-0.157538081528-0.1411944236420.264523679982-0.302085146078-0.2535423542750.06999830668880.2576878744570.103872448489-0.07327412280450.217298418916-0.03014466253660.181482015322-18.912284731715.4262170639-34.1478879044
41.546506925810.3147103614590.01122566022884.357329889481.698098055032.86091795412-0.0963728412791-0.13288357538-0.06854270797260.5298102251280.222554417345-0.3598010461640.1017422171260.230716093324-0.04423603103130.1813795344660.0457933760436-0.02926814947990.1703476775590.002870048563250.15720498218712.70062697866.84732760723-27.016257574
52.93634511694-0.7043297424270.1077320628062.27086950896-0.08591639899440.00837177393507-0.1445791002780.001281938200950.6728424820890.0251567805117-0.04715205410670.225061787716-0.657450601885-0.4603280756330.1115121094590.4242521878890.130497879418-0.07053703643040.358607039637-0.01275054165620.419380874742-10.368371620132.0183749295-14.3190736728
63.0985141324-0.2469916476321.742428772330.853981448377-0.1410087752631.0775610438-0.108689841005-0.2327272917520.2924387208890.0951446515022-0.0687033901270.140055073282-0.253005663301-0.2149068650260.1084712575680.4240115501410.162991658502-0.02869224557280.324173202911-0.03560373658450.239117746848-19.402100951127.7194775488-13.3531878512
70.811989562581-0.211583358887-0.1112218865292.742152117930.9597049476222.6435593518-0.08196457348580.0923520793554-0.05846830206610.006556068274720.172819822524-0.221569318797-0.1153730755750.108694202753-0.04559091503830.171372377012-0.0518602974088-0.02662472249150.190203755587-0.006729917366890.18051060858910.826579441719.9487115221-23.9765889122
81.83027302261.06766998899-0.0347219763232.38727999836-1.325971788042.958080716520.0945778256395-0.107568488706-0.02644105104750.6895171228020.0273198479412-0.0983177391838-0.8147555827750.14272350757-0.07970965852080.420891610243-0.0848676787444-0.04515239816580.19541093282-0.01492190883150.1892382910669.8207607242428.2223769854-10.014539244
90.6486665953080.0609687196048-0.4624816069562.878072210170.5752149401140.7347572450470.1170061821120.138508406231-0.5725727396840.149939297703-0.259072303650.5419710538550.0993908695989-0.4801125249980.04258287612920.3535935163470.15912701321-0.1341455271470.499254718659-0.1394531930820.709489971997-50.29619377334.9026608387-23.7072166013
102.883952325320.5960456509420.1138979387581.846580139950.2409436022571.50440763369-0.178416901072-0.542287908406-0.590904121539-0.0185764035897-0.1538030520710.666999488757-0.197480804206-0.208628568960.2898395941050.3193040420130.0917842459554-0.0281895459760.490983341822-0.04872511580870.71910891375-50.798163339327.30646355-16.3627082659
117.962753082792.63040297871-0.7529703118655.86425005383-0.9880360253972.7627430147-0.300311134636-0.690700927033-0.819678608640.8274984476850.2477948487190.6807115785490.665274740907-0.4838844237730.006116609015690.4876275259520.047414280270.05602730889380.5243179810170.05203540851871.04603797636-50.83342248818.1346107389-17.9103588764
121.30101191725-0.271820180096-0.05242691914967.778971002-2.552117181892.07294990957-0.0006269503168510.0417518972686-0.1710237655660.1827678955630.143196208468-0.0945380769658-0.05887800341710.0569103185919-0.07498042447140.386148287520.172708633558-0.04306648782330.432677840352-0.06199082650150.389785645705-38.499842630241.7798738252-22.9356354673
133.434537568180.632278971617-0.4500631977271.35808618093-0.01046919564111.39069501229-0.0462275320960.221244270328-0.00297196915074-0.399377253251-0.06817004101240.147116505541-0.687493617512-0.201389229912-0.1691162029450.3535694075770.243547035439-0.125324138240.394290943007-0.08591950169510.51641766646-50.474909664754.3687399351-23.4236904657
140.600737334027-0.232931324370.2602993848691.185792767840.4162455630650.8063123310910.00245121952313-0.361952790458-0.323182388748-0.1424700925880.1897757743910.108617408770.511362473672-0.722767738594-0.03080154788890.478871575234-0.06173546393110.09142881635650.933161862109-0.002849660994061.14026808722-75.616964631731.2190838953-11.3564944396
150.749320172330.939811265897-0.4383815444691.17427048876-0.1777973111090.6650936592250.136716957575-0.171144735386-0.3210928525060.324897703121-0.3459507596560.8741225466420.0122875043527-0.1533674779410.2062875248980.4221582163660.0938996890320.1467530494050.679045022046-0.04805048832360.871740277541-64.965610446239.9853414923-9.25865867725
161.341969561261.32314370837-0.4358409136181.34402333435-0.4987439452561.10844825040.145005786193-0.350943130572-0.8541183871650.213155042845-0.5587443278921.10140394406-0.470272343175-0.0674979724379-0.0469241243310.3307691634320.1862290429820.1652312116610.703525340550.01293346275011.08586302771-65.364599382337.9728075655-13.0841946951
171.86450710054-0.340810078065-0.8599809940671.30883378047-0.4619256031941.848220987860.467610899373-0.154253161580.2701751685210.35683318522-0.2386982434221.25899669466-0.302746842596-0.504930219716-0.09343475806560.4599881501620.2778993450770.1055784240580.75200978999-0.1350326765041.09452653692-70.303976240646.5617416373-11.6688095853
181.167193522160.5863131980220.7051812044774.987102402071.982992547252.351027544150.1293271290140.310165113669-0.480316384943-0.281809998506-0.1730553329470.556727741742-0.0829685791716-0.22317529015-0.05504672013630.4303044254640.245505596461-0.1620978908720.572563411609-0.1582015617840.60766777708-49.678476423537.5740933088-30.4725307164
191.32674993592-0.363730705021-0.5857002507751.026113038880.7987043915621.357944143240.4702231905010.4814648701030.112107054075-0.832716829259-0.4344503277040.00625485797123-0.230566583572-0.06131833635320.05035738231550.7878810664220.364818991653-0.1362514320650.620809835055-0.05064962703110.468232076935-41.936309753842.6032600148-38.697912318
202.799569637071.49738559445-0.5582468466933.73375082707-0.9728625168241.666080064710.4273352081480.321797068357-0.265307960328-0.562958863631-0.4900443220630.0604397341390.288029213364-0.4935714798210.08639476015630.6578968428920.533127887967-0.1732612292660.718782317725-0.2156782942750.519565264609-37.33679713128.4596155158-39.5235592981
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'B' and (resid 78 through 190 )BB78 - 19078 - 190
22chain 'C' and (resid 1 through 13 )CC1 - 131 - 13
33chain 'D' and (resid 1 through 119 )DD1 - 1191 - 119
44chain 'D' and (resid 120 through 202 )DD120 - 202120 - 202
55chain 'E' and (resid 1 through 13 )EE1 - 131 - 13
66chain 'E' and (resid 14 through 119 )EE14 - 11914 - 119
77chain 'E' and (resid 120 through 210 )EE120 - 210120 - 210
88chain 'E' and (resid 211 through 240 )EE211 - 240211 - 240
99chain 'A' and (resid 3 through 26 )AA3 - 261 - 24
1010chain 'A' and (resid 27 through 45 )AA27 - 4525 - 43
1111chain 'A' and (resid 46 through 55 )AA46 - 5544 - 53
1212chain 'A' and (resid 56 through 76 )AA56 - 7654 - 74
1313chain 'A' and (resid 77 through 87 )AA77 - 8775 - 85
1414chain 'A' and (resid 88 through 102 )AA88 - 10286 - 100
1515chain 'A' and (resid 103 through 133 )AA103 - 133101 - 131
1616chain 'A' and (resid 134 through 166 )AA134 - 166132 - 164
1717chain 'A' and (resid 167 through 181 )AA167 - 181165 - 179
1818chain 'B' and (resid 1 through 32 )BB1 - 321 - 32
1919chain 'B' and (resid 33 through 64 )BB33 - 6433 - 64
2020chain 'B' and (resid 65 through 77 )BB65 - 7765 - 77

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