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Yorodumi- PDB-8pjf: Human Leukocyte Antigen class II allotype DR1 presenting P11T->R ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8pjf | ||||||
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Title | Human Leukocyte Antigen class II allotype DR1 presenting P11T->R modified influenza A virus haemagglutinin (HA)306-318 PKYVKQNTLKLAR | ||||||
Components |
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Keywords | IMMUNE SYSTEM / HLA-II / HLA-DR / HLA-DR1 / human leukocyte antigen / major histocompatibility complex / major histocompatibility complex class 2 / influenza A virus / flu / haemagglutinin / HA / infection / vaccine | ||||||
Function / homology | Function and homology information regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / positive regulation of T cell mediated immune response to tumor cell / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation ...regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / positive regulation of T cell mediated immune response to tumor cell / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of memory T cell differentiation / CD4 receptor binding / positive regulation of monocyte differentiation / positive regulation of kinase activity / inflammatory response to antigenic stimulus / intermediate filament / T-helper 1 type immune response / polysaccharide binding / transport vesicle membrane / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / positive regulation of insulin secretion involved in cellular response to glucose stimulus / macrophage differentiation / humoral immune response / negative regulation of type II interferon production / Generation of second messenger molecules / immunological synapse / epidermis development / PD-1 signaling / trans-Golgi network membrane / T cell receptor binding / detection of bacterium / negative regulation of T cell proliferation / negative regulation of inflammatory response to antigenic stimulus / MHC class II antigen presentation / viral budding from plasma membrane / cognition / lumenal side of endoplasmic reticulum membrane / protein tetramerization / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / structural constituent of cytoskeleton / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / positive regulation of T cell mediated cytotoxicity / peptide antigen binding / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / Interferon gamma signaling / endocytic vesicle membrane / positive regulation of T cell activation / Downstream TCR signaling / MHC class II protein complex binding / late endosome membrane / T cell receptor signaling pathway / early endosome membrane / clathrin-dependent endocytosis of virus by host cell / positive regulation of canonical NF-kappaB signal transduction / adaptive immune response / positive regulation of MAPK cascade / positive regulation of ERK1 and ERK2 cascade / lysosome / positive regulation of viral entry into host cell / host cell surface receptor binding / immune response / positive regulation of protein phosphorylation / lysosomal membrane / Golgi membrane / external side of plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / positive regulation of DNA-templated transcription / cell surface / signal transduction / extracellular space / extracellular exosome / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Influenza A virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.48 Å | ||||||
Authors | MacLachlan, B.J. / Wall, A. / Greenshields-Watson, A.L. / Cole, D.K. / Rizkallah, P.J. / Godkin, A.J. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Cell Rep / Year: 2024 Title: A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4 + T cell activation. Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, ...Authors: Hulin-Curtis, S. / Geary, J.K. / MacLachlan, B.J. / Altmann, D.M. / Baillon, L. / Cole, D.K. / Greenshields-Watson, A. / Hesketh, S.J. / Humphreys, I.R. / Jones, I.M. / Lauder, S.N. / Mason, G.H. / Smart, K. / Scourfield, D.O. / Scott, J. / Sukhova, K. / Stanton, R.J. / Wall, A. / Rizkallah, P.J. / Barclay, W.S. / Gallimore, A. / Godkin, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8pjf.cif.gz | 227 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8pjf.ent.gz | 151.6 KB | Display | PDB format |
PDBx/mmJSON format | 8pjf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8pjf_validation.pdf.gz | 476.4 KB | Display | wwPDB validaton report |
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Full document | 8pjf_full_validation.pdf.gz | 479 KB | Display | |
Data in XML | 8pjf_validation.xml.gz | 20.4 KB | Display | |
Data in CIF | 8pjf_validation.cif.gz | 29.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pj/8pjf ftp://data.pdbj.org/pub/pdb/validation_reports/pj/8pjf | HTTPS FTP |
-Related structure data
Related structure data | 8pjeC 8pjgC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-HLA class II histocompatibility antigen, ... , 2 types, 2 molecules AB
#1: Protein | Mass: 21562.426 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRA, HLA-DRA1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01903 |
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#2: Protein | Mass: 22487.186 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRB1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01911 |
-Protein/peptide , 1 types, 1 molecules C
#3: Protein/peptide | Mass: 1562.896 Da / Num. of mol.: 1 / Mutation: T318R / Source method: obtained synthetically / Source: (synth.) Influenza A virus / References: UniProt: P03435 |
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-Non-polymers , 3 types, 328 molecules
#4: Chemical | ChemComp-SO4 / #5: Chemical | ChemComp-EDO / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 56.7 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.1 M MES pH 7.0, 25 % PEG4000, 0.2 M ammonium sulphate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97625 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jul 23, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 1.48→54.14 Å / Num. obs: 83579 / % possible obs: 99.69 % / Redundancy: 7.1 % / Biso Wilson estimate: 20.19 Å2 / CC1/2: 0.998 / CC star: 1 / Rmerge(I) obs: 0.0797 / Rpim(I) all: 0.0324 / Rrim(I) all: 0.0861 / Net I/σ(I): 12.79 |
Reflection shell | Resolution: 1.48→1.53 Å / Redundancy: 7.1 % / Rmerge(I) obs: 1.627 / Mean I/σ(I) obs: 1.49 / Num. unique obs: 8248 / CC1/2: 0.527 / CC star: 0.831 / Rpim(I) all: 0.653 / Rrim(I) all: 1.755 / % possible all: 99.92 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.48→54.14 Å / SU ML: 0.168 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.1655 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.34 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.48→54.14 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION
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