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Yorodumi- PDB-8ols: Structure of Oceanobacillus iheyensis group II intron in the pres... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8ols | |||||||||||||||||||||||||||
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Title | Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and intronistat B | |||||||||||||||||||||||||||
Components | Group IIC intron | |||||||||||||||||||||||||||
Keywords | RNA / RIBOZYME / METALLOENZYME / SELF-SPLICING / RETROTRANSPOSITION | |||||||||||||||||||||||||||
Function / homology | : / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) Function and homology information | |||||||||||||||||||||||||||
Biological species | Oceanobacillus iheyensis (bacteria) | |||||||||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | |||||||||||||||||||||||||||
Authors | Silvestri, I. / Marcia, M. | |||||||||||||||||||||||||||
Funding support | France, Italy, 8items
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Citation | Journal: Nat Commun / Year: 2024 Title: Targeting the conserved active site of splicing machines with specific and selective small molecule modulators. Authors: Silvestri, I. / Manigrasso, J. / Andreani, A. / Brindani, N. / Mas, C. / Reiser, J.B. / Vidossich, P. / Martino, G. / McCarthy, A.A. / De Vivo, M. / Marcia, M. | |||||||||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ols.cif.gz | 237 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ols.ent.gz | 175.1 KB | Display | PDB format |
PDBx/mmJSON format | 8ols.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ols_validation.pdf.gz | 737.3 KB | Display | wwPDB validaton report |
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Full document | 8ols_full_validation.pdf.gz | 792.4 KB | Display | |
Data in XML | 8ols_validation.xml.gz | 20.3 KB | Display | |
Data in CIF | 8ols_validation.cif.gz | 28.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ol/8ols ftp://data.pdbj.org/pub/pdb/validation_reports/ol/8ols | HTTPS FTP |
-Related structure data
Related structure data | 8olvC 8olwC 8olyC 8olzC 8om0C 8ruhC 8ruiC 8rujC 8rukC 8rulC 8rumC 8runC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-RNA chain , 1 types, 1 molecules A
#1: RNA chain | Mass: 127991.789 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Oceanobacillus iheyensis (bacteria) |
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-Non-polymers , 6 types, 58 molecules
#2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-K / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-VTE / ~{ | Mass: 410.420 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C22H22N2O6 / Feature type: SUBJECT OF INVESTIGATION #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.74 Å3/Da / Density % sol: 67.08 % |
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Crystal grow | Temperature: 303.15 K / Method: vapor diffusion, hanging drop Details: 100 mM magnesium acetate, 200 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-1 / Wavelength: 0.96 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Sep 1, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96 Å / Relative weight: 1 |
Reflection | Resolution: 3→39.7 Å / Num. obs: 38577 / % possible obs: 99.31 % / Redundancy: 5.4 % / CC1/2: 1 / Rmerge(I) obs: 0.044 / Net I/σ(I): 18.58 |
Reflection shell | Resolution: 3→3.1 Å / Num. unique obs: 3799 / CC1/2: 0.35 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3→39.69 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.938 / SU B: 18.692 / SU ML: 0.317 / Cross valid method: THROUGHOUT / ESU R: 1.044 / ESU R Free: 0.358 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 138.89 Å2
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Refinement step | Cycle: 1 / Resolution: 3→39.69 Å
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