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Yorodumi- PDB-6t3n: Structure of Oceanobacillus iheyensis group II intron G-mutant (C... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6t3n | ||||||||||||
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Title | Structure of Oceanobacillus iheyensis group II intron G-mutant (C289G/C358G/G385C) in the presence of Na+, Mg2+ and 5'-exon | ||||||||||||
Components | Group IIC Intron Ribozyme | ||||||||||||
Keywords | RNA / Ribozyme / Self-splicing / Retrotransposition / Spliceosome | ||||||||||||
Function / homology | RNA / RNA (> 10) / RNA (> 100) Function and homology information | ||||||||||||
Biological species | Oceanobacillus iheyensis HTE831 (bacteria) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.22 Å | ||||||||||||
Authors | Marcia, M. / Pyle, A.M. | ||||||||||||
Funding support | France, United States, 3items
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Citation | Journal: Nat Commun / Year: 2020 Title: Visualizing group II intron dynamics between the first and second steps of splicing. Authors: Manigrasso, J. / Chillon, I. / Genna, V. / Vidossich, P. / Somarowthu, S. / Pyle, A.M. / De Vivo, M. / Marcia, M. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6t3n.cif.gz | 235.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6t3n.ent.gz | 176.2 KB | Display | PDB format |
PDBx/mmJSON format | 6t3n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t3/6t3n ftp://data.pdbj.org/pub/pdb/validation_reports/t3/6t3n | HTTPS FTP |
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-Related structure data
Related structure data | 6t3kC 6t3rC 6t3sC 3igiS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 129279.547 Da / Num. of mol.: 1 / Fragment: domains 1-5 and 5'-exon / Mutation: C289G/C358G/G385C / Source method: obtained synthetically / Details: in vitro transcription system / Source: (synth.) Oceanobacillus iheyensis HTE831 (bacteria) | ||||||||
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#2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-NA / #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.61 Å3/Da / Density % sol: 65.91 % / Mosaicity: 0.15 ° |
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Crystal grow | Temperature: 303 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 50 mM Na-HEPES pH 7.0, 100 mM magnesium acetate, 150 mM sodium chloride, 4% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 14, 2013 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: SINGLE CRYSTAL SI(220) SIDE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.219→32.15 Å / Num. all: 30536 / Num. obs: 30536 / % possible obs: 98.6 % / Redundancy: 4.8 % / Rpim(I) all: 0.039 / Rrim(I) all: 0.089 / Rsym value: 0.069 / Net I/av σ(I): 8.2 / Net I/σ(I): 14.5 / Num. measured all: 147818 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3IGI Resolution: 3.22→32.15 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.922 / SU B: 20.359 / SU ML: 0.336 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.432 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 385.07 Å2 / Biso mean: 132.355 Å2 / Biso min: 28.45 Å2
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Refinement step | Cycle: final / Resolution: 3.22→32.15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.22→3.302 Å / Rfactor Rfree error: 0
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