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Structure paper

TitleVisualizing group II intron dynamics between the first and second steps of splicing.
Journal, issue, pagesNat Commun, Vol. 11, Page 2837-2837, Year 2020
Publish dateOct 11, 2019 (structure data deposition date)
AuthorsManigrasso, J. / Chillon, I. / Genna, V. / Vidossich, P. / Somarowthu, S. / Pyle, A.M. / De Vivo, M. / Marcia, M.
External linksNat Commun / PubMed:32503992
MethodsX-ray diffraction
Resolution3.22 - 3.57 Å
Structure data

PDB-6t3k:
Structure of Oceanobacillus iheyensis group II intron G-mutant (C289G/C358G/G385C) in the presence of K+, Mg2+ and 5'-exon
Method: X-RAY DIFFRACTION / Resolution: 3.44 Å

PDB-6t3n:
Structure of Oceanobacillus iheyensis group II intron G-mutant (C289G/C358G/G385C) in the presence of Na+, Mg2+ and 5'-exon
Method: X-RAY DIFFRACTION / Resolution: 3.22 Å

PDB-6t3r:
Structure of Oceanobacillus iheyensis group II intron U-mutant (C289U/C358U/G385A) in the presence of K+, Mg2+ and 5'-exon
Method: X-RAY DIFFRACTION / Resolution: 3.57 Å

PDB-6t3s:
Structure of Oceanobacillus iheyensis group II intron U-mutant (C289U/C358U/G385A) in the presence of Na+, Mg2+ and 5'-exon
Method: X-RAY DIFFRACTION / Resolution: 3.28 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-SPM:
SPERMINE

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

Source
  • oceanobacillus iheyensis (bacteria)
  • oceanobacillus iheyensis hte831 (bacteria)
KeywordsRNA / Ribozyme / Self-splicing / Retrotransposition / Spliceosome

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