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- PDB-8i13: Cryo-EM structure of 6-subunit Smc5/6 -

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Basic information

Entry
Database: PDB / ID: 8i13
TitleCryo-EM structure of 6-subunit Smc5/6
Components
  • (Non-structural maintenance of chromosomes element ...) x 2
  • MMS21 isoform 1
  • NSE3 isoform 1
  • SMC6 isoform 1
  • Structural maintenance of chromosomes protein 5
KeywordsCELL CYCLE
Function / homology
Function and homology information


Smc5-Smc6 complex / SUMO ligase activity / protein sumoylation / chromosome segregation / double-strand break repair via homologous recombination / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / site of double-strand break / single-stranded DNA binding / DNA recombination ...Smc5-Smc6 complex / SUMO ligase activity / protein sumoylation / chromosome segregation / double-strand break repair via homologous recombination / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / site of double-strand break / single-stranded DNA binding / DNA recombination / damaged DNA binding / DNA repair / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / zinc ion binding / ATP binding / nucleus / metal ion binding
Similarity search - Function
Non-structural maintenance of chromosomes element 1 / Zinc finger, RING-like / Nse1 non-SMC component of SMC5-6 complex / RING-like domain / Non-structural maintenance of chromosome element 4, C-terminal / Nse4/EID family / Nse4/EID protein, Nse3/MAGE-binding domain / Nse4 C-terminal / Binding domain of Nse4/EID3 to Nse3-MAGE / E3 SUMO-protein ligase Nse2 (Mms21) ...Non-structural maintenance of chromosomes element 1 / Zinc finger, RING-like / Nse1 non-SMC component of SMC5-6 complex / RING-like domain / Non-structural maintenance of chromosome element 4, C-terminal / Nse4/EID family / Nse4/EID protein, Nse3/MAGE-binding domain / Nse4 C-terminal / Binding domain of Nse4/EID3 to Nse3-MAGE / E3 SUMO-protein ligase Nse2 (Mms21) / Zinc-finger of the MIZ type in Nse subunit / Structural maintenance of chromosomes protein 5 / Zinc finger, MIZ-type / Zinc finger SP-RING-type profile. / MAGE homology domain / Melanoma-associated antigen / MAGE homology domain, winged helix WH1 motif / MAGE homology domain, winged helix WH2 motif / MAGE homology domain / Melanoma-associated antigen / Rad50/SbcC-type AAA domain / AAA domain / RecF/RecN/SMC, N-terminal / RecF/RecN/SMC N terminal domain / Zinc finger, RING/FYVE/PHD-type / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Non-structural maintenance of chromosomes element 4 / Structural maintenance of chromosomes protein 5 / SMC6 isoform 1 / Non-structural maintenance of chromosomes element 1 homolog / MMS21 isoform 1 / NSE3 isoform 1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 6.9 Å
AuthorsQian, L. / Jun, Z. / Xiang, Z. / Cheng, T. / Zhaoning, W. / Duo, J. / Zhenguo, C. / Wang, L.
Funding support1items
OrganizationGrant numberCountry
Other government
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2024
Title: Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
Authors: Qian, L. / Jun, Z. / Xiang, Z. / Wang, Z. / Tong, C. / Duo, J. / Zhenguo, C. / Wang, L.
History
DepositionJan 12, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 26, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: SMC6 isoform 1
A: Structural maintenance of chromosomes protein 5
C: MMS21 isoform 1
F: Non-structural maintenance of chromosomes element 1 homolog
G: NSE3 isoform 1
H: Non-structural maintenance of chromosomes element 4


Theoretical massNumber of molelcules
Total (without water)403,4006
Polymers403,4006
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein , 4 types, 4 molecules BACG

#1: Protein SMC6 isoform 1


Mass: 128199.727 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: SMC6 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A8H4BXH7
#2: Protein Structural maintenance of chromosomes protein 5


Mass: 126237.164 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: SMC5 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A6V8S000
#3: Protein MMS21 isoform 1


Mass: 30388.336 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: MMS21 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A8H8ULJ5
#5: Protein NSE3 isoform 1


Mass: 34005.531 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: NSE3 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A8H8UNJ0

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Non-structural maintenance of chromosomes element ... , 2 types, 2 molecules FH

#4: Protein Non-structural maintenance of chromosomes element 1 homolog


Mass: 38373.387 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: NSE1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A8H4F9V3
#6: Protein Non-structural maintenance of chromosomes element 4


Mass: 46195.945 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: NSE4 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: A0A6L0Z6W9

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of 6-subunit Smc5/6 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Source (recombinant)Organism: Spodoptera frugiperda (fall armyworm)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: DARK FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

CTF correctionType: NONE
3D reconstructionResolution: 6.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1000000 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00426229
ELECTRON MICROSCOPYf_angle_d0.79435265
ELECTRON MICROSCOPYf_dihedral_angle_d5.1413477
ELECTRON MICROSCOPYf_chiral_restr0.0473980
ELECTRON MICROSCOPYf_plane_restr0.0064575

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