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- PDB-8hqs: Cryo-EM structure of 8-subunit Smc5/6 head region -

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Basic information

Entry
Database: PDB / ID: 8hqs
TitleCryo-EM structure of 8-subunit Smc5/6 head region
Components
  • (Non-structural maintenance of chromosome element ...) x 3
  • (Structural maintenance of chromosomes protein ...) x 2
  • DNA repair protein KRE29
  • Non-structural maintenance of chromosomes element 1
KeywordsCELL CYCLE
Function / homology
Function and homology information


Smc5-Smc6 complex / resolution of DNA recombination intermediates / DNA double-strand break attachment to nuclear envelope / chromosome separation / Platelet degranulation / SUMOylation of DNA damage response and repair proteins / chromatin looping / postreplication repair / recombinational repair / regulation of telomere maintenance ...Smc5-Smc6 complex / resolution of DNA recombination intermediates / DNA double-strand break attachment to nuclear envelope / chromosome separation / Platelet degranulation / SUMOylation of DNA damage response and repair proteins / chromatin looping / postreplication repair / recombinational repair / regulation of telomere maintenance / protein sumoylation / double-strand break repair via homologous recombination / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / site of double-strand break / single-stranded DNA binding / chromosome, telomeric region / damaged DNA binding / DNA repair / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / mitochondrion / ATP binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
Non-structural maintenance of chromosomes element 1 / Zinc finger, RING-like / Nse1 non-SMC component of SMC5-6 complex / RING-like domain / Non-structural maintenance of chromosome element 4, C-terminal / Nse4/EID family / Nse4/EID protein, Nse3/MAGE-binding domain / Nse4 C-terminal / Binding domain of Nse4/EID3 to Nse3-MAGE / DNA repair protein Nse5/Nse6 ...Non-structural maintenance of chromosomes element 1 / Zinc finger, RING-like / Nse1 non-SMC component of SMC5-6 complex / RING-like domain / Non-structural maintenance of chromosome element 4, C-terminal / Nse4/EID family / Nse4/EID protein, Nse3/MAGE-binding domain / Nse4 C-terminal / Binding domain of Nse4/EID3 to Nse3-MAGE / DNA repair protein Nse5/Nse6 / DNA repair proteins Nse5 and Nse6 / Structural maintenance of chromosomes protein 5 / MAGE homology domain / Melanoma-associated antigen / MAGE homology domain, winged helix WH1 motif / MAGE homology domain, winged helix WH2 motif / MAGE homology domain / Melanoma-associated antigen / Rad50/SbcC-type AAA domain / AAA domain / RecF/RecN/SMC, N-terminal / RecF/RecN/SMC N terminal domain / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DNA repair protein KRE29 / Non-structural maintenance of chromosome element 4 / Non-structural maintenance of chromosome element 5 / Non-structural maintenance of chromosome element 3 / Non-structural maintenance of chromosomes element 1 / Structural maintenance of chromosomes protein 5 / Structural maintenance of chromosomes protein 6
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288C (yeast)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsQian, L. / Jun, Z. / Xiang, Z. / Tong, C. / Wang, Z. / Duo, J. / Zhenguo, C. / Wang, L.
Funding support1items
OrganizationGrant numberCountry
Other government
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2024
Title: Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
Authors: Qian, L. / Jun, Z. / Xiang, Z. / Wang, Z. / Tong, C. / Duo, J. / Zhenguo, C. / Wang, L.
History
DepositionDec 14, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 19, 2024Provider: repository / Type: Initial release
Revision 1.1Jun 26, 2024Group: Data collection / Database references / Category: citation / citation_author / em_admin
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name / _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Structural maintenance of chromosomes protein 5
B: Structural maintenance of chromosomes protein 6
D: DNA repair protein KRE29
E: Non-structural maintenance of chromosome element 5
F: Non-structural maintenance of chromosomes element 1
G: Non-structural maintenance of chromosome element 3
H: Non-structural maintenance of chromosome element 4


Theoretical massNumber of molelcules
Total (without water)490,9287
Polymers490,9287
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Structural maintenance of chromosomes protein ... , 2 types, 2 molecules AB

#1: Protein Structural maintenance of chromosomes protein 5


Mass: 126237.164 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: SMC5 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q08204
#2: Protein Structural maintenance of chromosomes protein 6


Mass: 128199.727 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: SMC6 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q12749

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Protein , 2 types, 2 molecules DF

#3: Protein DNA repair protein KRE29 / Killer toxin-resistance protein 29


Mass: 53836.758 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: KRE29 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P40026
#5: Protein Non-structural maintenance of chromosomes element 1 / Non-SMC element 1


Mass: 38373.387 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: NSE1 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: Q07913, RING-type E3 ubiquitin transferase

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Non-structural maintenance of chromosome element ... , 3 types, 3 molecules EGH

#4: Protein Non-structural maintenance of chromosome element 5 / Non-SMC element 5


Mass: 64079.609 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: NSE5 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q03718
#6: Protein Non-structural maintenance of chromosome element 3 / Non-SMC element 3


Mass: 34005.531 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: NSE3 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q05541
#7: Protein Non-structural maintenance of chromosome element 4 / Non-SMC element 4 / Protein QRI2


Mass: 46195.945 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288C (yeast) / Strain: S288c / Gene: NSE4 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P43124

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of 8-subunit Smc5/6 head region / Type: COMPLEX / Entity ID: #5-#6, #1-#4, #7 / Source: RECOMBINANT
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Source (recombinant)Organism: Spodoptera frugiperda (fall armyworm)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: DARK FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 546112 / Symmetry type: POINT

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