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TitleCryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
Journal, issue, pagesNat Struct Mol Biol, Year 2024
Publish dateJun 18, 2024
AuthorsQian Li / Jun Zhang / Cory Haluska / Xiang Zhang / Lei Wang / Guangfeng Liu / Zhaoning Wang / Duo Jin / Tong Cheng / Hongxia Wang / Yuan Tian / Xiangxi Wang / Lei Sun / Xiaolan Zhao / Zhenguo Chen / Lanfeng Wang /
PubMed AbstractSmc5/6 is a member of the eukaryotic structural maintenance of chromosomes (SMC) family of complexes with important roles in genome maintenance and viral restriction. However, limited structural ...Smc5/6 is a member of the eukaryotic structural maintenance of chromosomes (SMC) family of complexes with important roles in genome maintenance and viral restriction. However, limited structural understanding of Smc5/6 hinders the elucidation of its diverse functions. Here, we report cryo-EM structures of the budding yeast Smc5/6 complex in eight-subunit, six-subunit and five-subunit states. Structural maps throughout the entire length of these complexes reveal modularity and key elements in complex assembly. We show that the non-SMC element (Nse)2 subunit supports the overall shape of the complex and uses a wedge motif to aid the stability and function of the complex. The Nse6 subunit features a flexible hook region for attachment to the Smc5 and Smc6 arm regions, contributing to the DNA repair roles of the complex. Our results also suggest a structural basis for the opposite effects of the Nse1-3-4 and Nse5-6 subcomplexes in regulating Smc5/6 ATPase activity. Collectively, our integrated structural and functional data provide a framework for understanding Smc5/6 assembly and function.
External linksNat Struct Mol Biol / PubMed:38890552
MethodsEM (single particle)
Resolution3.2 - 8.5 Å
Structure data

33914

7ylm

EMDB-33914, PDB-7ylm:
Cryo-EM structure of 8-subunit Smc5/6 hinge region
Method: EM (single particle) / Resolution: 6.17 Å

33927

7ymd

EMDB-33927, PDB-7ymd:
Cryo-EM structure of Nse1/3/4
Method: EM (single particle) / Resolution: 4.176 Å

34025

7yqh

EMDB-34025, PDB-7yqh:
Cryo-EM structure of 8-subunit Smc5/6
Method: EM (single particle) / Resolution: 5.6 Å

34953

8hqs

EMDB-34953, PDB-8hqs:
Cryo-EM structure of 8-subunit Smc5/6 head region
Method: EM (single particle) / Resolution: 3.2 Å

35116

8i13

EMDB-35116, PDB-8i13:
Cryo-EM structure of 6-subunit Smc5/6
Method: EM (single particle) / Resolution: 6.9 Å

35128

8i21

EMDB-35128, PDB-8i21:
Cryo-EM structure of 6-subunit Smc5/6 arm region
Method: EM (single particle) / Resolution: 6.02 Å

35184

8i4u

EMDB-35184, PDB-8i4u:
Cryo-EM structure of 5-subunit Smc5/6 hinge region
Method: EM (single particle) / Resolution: 6.73 Å

35185

8i4v

EMDB-35185, PDB-8i4v:
Cryo-EM structure of 5-subunit Smc5/6 arm region
Method: EM (single particle) / Resolution: 5.97 Å

35186

8i4w

EMDB-35186, PDB-8i4w:
Cryo-EM structure of 5-subunit Smc5/6 head region
Method: EM (single particle) / Resolution: 6.01 Å

35187

8i4x

EMDB-35187, PDB-8i4x:
Cryo-EM structure of 5-subunit Smc5/6
Method: EM (single particle) / Resolution: 8.5 Å

37584

8wjl

EMDB-37584, PDB-8wjl:
Cryo-EM structure of 6-subunit Smc5/6 hinge region
Method: EM (single particle) / Resolution: 6.15 Å

37586

8wjn

EMDB-37586, PDB-8wjn:
Cryo-EM structure of 6-subunit Smc5/6 head region
Method: EM (single particle) / Resolution: 5.58 Å

37587

8wjo

EMDB-37587, PDB-8wjo:
Cryo-EM structure of 8-subunit Smc5/6 arm region
Method: EM (single particle) / Resolution: 6.04 Å

EMDB-60245: Smc5/6-5mer consensus map
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-60246: Smc5/6-6mer consensus map
Method: EM (single particle) / Resolution: 6.9 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsCELL CYCLE

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