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- PDB-8gkm: A1 Leu graft + Leu: Adenylation domain 1 core construct from teic... -

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基本情報

登録情報
データベース: PDB / ID: 8gkm
タイトルA1 Leu graft + Leu: Adenylation domain 1 core construct from teicoplanin biosynthesis, leucine selection pocket graft; leucine bound
要素
  • MbtH-like short polypeptide
  • Non-ribosomal peptide synthetase
キーワードLIGASE / AMP-binding enzyme / NRPS / Adenylation domain
機能・相同性
機能・相同性情報


siderophore biosynthetic process / amino acid activation for nonribosomal peptide biosynthetic process / secondary metabolite biosynthetic process / lipid biosynthetic process / phosphopantetheine binding / catalytic activity / cytosol
類似検索 - 分子機能
MbtH-like protein / MbtH-like domain / MbtH-like domain superfamily / MbtH-like protein / MbtH-like protein / Non-ribosomal peptide synthase / Condensation domain / Condensation domain / Amino acid adenylation domain / AMP-binding enzyme, C-terminal domain ...MbtH-like protein / MbtH-like domain / MbtH-like domain superfamily / MbtH-like protein / MbtH-like protein / Non-ribosomal peptide synthase / Condensation domain / Condensation domain / Amino acid adenylation domain / AMP-binding enzyme, C-terminal domain / AMP-binding enzyme C-terminal domain / Chloramphenicol acetyltransferase-like domain superfamily / AMP-binding, conserved site / Putative AMP-binding domain signature. / Polyketide synthase, phosphopantetheine-binding domain / Phosphopantetheine attachment site / AMP-dependent synthetase/ligase / AMP-binding enzyme, C-terminal domain superfamily / AMP-binding enzyme / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain
類似検索 - ドメイン・相同性
ACETIC ACID / LEUCINE / DI(HYDROXYETHYL)ETHER / MbtH-like short polypeptide / Non-ribosomal peptide synthetase
類似検索 - 構成要素
生物種Actinoplanes teichomyceticus (バクテリア)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 1.89 Å
データ登録者Hansen, M.H. / Cryle, M.J.
資金援助 オーストラリア, 2件
組織認可番号
Australian Research Council (ARC)DP190101272 オーストラリア
Australian Research Council (ARC)DP210101752 オーストラリア
引用ジャーナル: Nat Commun / : 2023
タイトル: Resurrecting ancestral antibiotics: unveiling the origins of modern lipid II targeting glycopeptides.
著者: Hansen, M.H. / Adamek, M. / Iftime, D. / Petras, D. / Schuseil, F. / Grond, S. / Stegmann, E. / Cryle, M.J. / Ziemert, N.
履歴
登録2023年3月20日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02023年12月6日Provider: repository / タイプ: Initial release
改定 1.12023年12月13日Group: Database references / カテゴリ: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
A: Non-ribosomal peptide synthetase
B: Non-ribosomal peptide synthetase
C: MbtH-like short polypeptide
D: MbtH-like short polypeptide
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)100,0169
ポリマ-99,5644
非ポリマー4525
6,449358
1
A: Non-ribosomal peptide synthetase
D: MbtH-like short polypeptide
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)50,1026
ポリマ-49,7822
非ポリマー3204
362
タイプ名称対称操作
identity operation1_555x,y,z1
2
B: Non-ribosomal peptide synthetase
C: MbtH-like short polypeptide
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)49,9133
ポリマ-49,7822
非ポリマー1311
362
タイプ名称対称操作
identity operation1_555x,y,z1
単位格子
Length a, b, c (Å)41.805, 123.415, 175.171
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP22121
Space group name HallP22ab(z,x,y)
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y+1/2,-z+1/2
#4: -x,-y+1/2,z+1/2
非結晶学的対称性 (NCS)NCSドメイン:
IDEns-ID詳細
d_1ens_1(chain "A" and (resid 2 through 38 or resid 40...
d_2ens_1(chain "B" and (resid 2 through 38 or resid 40...
d_1ens_2chain "C"
d_2ens_2(chain "D" and resid 3 through 62)

NCSドメイン領域:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1SERGLYA1 - 37
d_12ens_1LEUVALA39 - 48
d_13ens_1ARGASPA50 - 63
d_14ens_1VALPROA65 - 88
d_15ens_1VALASPA90 - 123
d_16ens_1ALASERA129 - 146
d_17ens_1ILEGLYA148 - 230
d_18ens_1PHEMETA232 - 284
d_19ens_1ALAGLYA286 - 304
d_110ens_1PROARGA306 - 343
d_111ens_1ALAGLYA345 - 382
d_112ens_1ALAASPA384 - 385
d_113ens_1LLB
d_21ens_1SERGLYE1 - 37
d_22ens_1LEUVALE39 - 48
d_23ens_1ARGASPE50 - 63
d_24ens_1VALPROE65 - 88
d_25ens_1VALASPE90 - 123
d_26ens_1ALASERE126 - 143
d_27ens_1ILEGLYE145 - 227
d_28ens_1PHEMETE229 - 281
d_29ens_1ALAGLYE283 - 301
d_210ens_1PROARGE303 - 340
d_211ens_1ALAGLYE342 - 379
d_212ens_1ALAASPE381 - 382
d_213ens_1LLF
d_11ens_2THRLYSG - A1 - 60
d_21ens_2THRLYSH - B1 - 60

NCSアンサンブル:
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixベクター
1given(-0.999699504518, 0.0233648366029, 0.00741519235776), (0.0230304746735, 0.998836936611, -0.0423600200404), (-0.00839630296574, -0.0421765156459, -0.999074893902)37.890090653, 1.10008697741, 75.6103751663
2given(-0.994273634086, 0.0845652711387, -0.0653349483705), (0.087692943041, 0.995057809064, -0.0465822322481), (0.0610728114805, -0.0520448992472, -0.996775521449)40.311342025, -0.81764554267, 73.7686093374

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要素

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タンパク質 , 2種, 4分子 ABCD

#1: タンパク質 Non-ribosomal peptide synthetase


分子量: 42145.809 Da / 分子数: 2 / 変異: H237Y, L287M, L295M / 由来タイプ: 組換発現
由来: (組換発現) Actinoplanes teichomyceticus (バクテリア)
遺伝子: tcp9 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: Q70AZ9
#2: タンパク質 MbtH-like short polypeptide


分子量: 7636.309 Da / 分子数: 2 / 由来タイプ: 組換発現
由来: (組換発現) Actinoplanes teichomyceticus (バクテリア)
遺伝子: tcp13 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: Q70AZ5

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非ポリマー , 5種, 363分子

#3: 化合物 ChemComp-LEU / LEUCINE / ロイシン


タイプ: L-peptide linking / 分子量: 131.173 Da / 分子数: 2 / 由来タイプ: 合成 / : C6H13NO2 / タイプ: SUBJECT OF INVESTIGATION
#4: 化合物 ChemComp-PEG / DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル


分子量: 106.120 Da / 分子数: 1 / 由来タイプ: 合成 / : C4H10O3
#5: 化合物 ChemComp-ACY / ACETIC ACID / 酢酸


分子量: 60.052 Da / 分子数: 1 / 由来タイプ: 合成 / : C2H4O2
#6: 化合物 ChemComp-NA / SODIUM ION / ナトリウムカチオン


分子量: 22.990 Da / 分子数: 1 / 由来タイプ: 合成 / : Na
#7: 水 ChemComp-HOH / water


分子量: 18.015 Da / 分子数: 358 / 由来タイプ: 天然 / : H2O

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詳細

研究の焦点であるリガンドがあるかY

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 2.27 Å3/Da / 溶媒含有率: 45.8 %
結晶化温度: 293.15 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6
詳細: 0.1 M MMT (L-Malic acid, MES and Tris in a ratio of 1:2:2), pH 6 and 25% w/v PEG 1500

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データ収集

回折平均測定温度: 100 K / Serial crystal experiment: N
放射光源由来: シンクロトロン / サイト: Australian Synchrotron / ビームライン: MX2 / 波長: 0.9536 Å
検出器タイプ: DECTRIS EIGER X 16M / 検出器: PIXEL / 日付: 2019年10月11日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 0.9536 Å / 相対比: 1
反射解像度: 1.89→43.79 Å / Num. obs: 73788 / % possible obs: 99.85 % / 冗長度: 2 % / Biso Wilson estimate: 28.7 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.03607 / Rsym value: 0.03607 / Net I/σ(I): 9.93
反射 シェル解像度: 1.89→1.958 Å / Rmerge(I) obs: 0.6918 / Num. unique obs: 7226 / CC1/2: 0.599 / Rpim(I) all: 0.6918

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解析

ソフトウェア
名称バージョン分類
PHENIX1.20.1_4487精密化
XDSデータ削減
Aimlessデータスケーリング
PHASER位相決定
精密化構造決定の手法: 分子置換 / 解像度: 1.89→43.79 Å / SU ML: 0.2053 / 交差検証法: FREE R-VALUE / σ(F): 1.33 / 位相誤差: 23.0546
立体化学のターゲット値: GeoStd + Monomer Library + CDL v1.2
Rfactor反射数%反射
Rfree0.2197 3701 5.02 %
Rwork0.1894 70087 -
obs0.1909 73788 99.5 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
原子変位パラメータBiso mean: 36.07 Å2
精密化ステップサイクル: LAST / 解像度: 1.89→43.79 Å
タンパク質核酸リガンド溶媒全体
原子数6651 0 30 358 7039
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.00376855
X-RAY DIFFRACTIONf_angle_d0.7489353
X-RAY DIFFRACTIONf_chiral_restr0.04791046
X-RAY DIFFRACTIONf_plane_restr0.00631245
X-RAY DIFFRACTIONf_dihedral_angle_d10.65482466
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDタイプRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.491193908965
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.627690336802
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.89-1.910.38021270.39282340X-RAY DIFFRACTION88.23
1.91-1.940.32371560.31832637X-RAY DIFFRACTION99.86
1.94-1.970.31321480.30872656X-RAY DIFFRACTION99.82
1.97-1.990.34891130.28672715X-RAY DIFFRACTION99.96
1.99-2.030.29721340.26572642X-RAY DIFFRACTION99.96
2.03-2.060.28951560.25192663X-RAY DIFFRACTION100
2.06-2.090.26541420.23652712X-RAY DIFFRACTION100
2.09-2.130.23131360.21482647X-RAY DIFFRACTION100
2.13-2.170.24971620.21272661X-RAY DIFFRACTION99.96
2.17-2.220.24431180.20532707X-RAY DIFFRACTION100
2.22-2.270.22971650.20382632X-RAY DIFFRACTION99.96
2.27-2.320.24951430.20112728X-RAY DIFFRACTION99.97
2.32-2.380.22111450.18962652X-RAY DIFFRACTION100
2.38-2.440.25771420.1822686X-RAY DIFFRACTION99.93
2.44-2.510.20031340.18692691X-RAY DIFFRACTION99.89
2.51-2.590.21261430.17652721X-RAY DIFFRACTION99.97
2.59-2.690.22111500.17252666X-RAY DIFFRACTION99.96
2.69-2.790.24221470.172744X-RAY DIFFRACTION99.97
2.79-2.920.18361430.16682674X-RAY DIFFRACTION99.96
2.92-3.080.15181530.16012713X-RAY DIFFRACTION99.97
3.08-3.270.19761390.16522747X-RAY DIFFRACTION100
3.27-3.520.23711200.16892783X-RAY DIFFRACTION99.97
3.52-3.870.19191420.16032726X-RAY DIFFRACTION99.97
3.87-4.430.18591520.16532754X-RAY DIFFRACTION99.93
4.43-5.590.18411420.17332814X-RAY DIFFRACTION99.83
5.59-43.790.24031490.2082976X-RAY DIFFRACTION99.74
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
11.82643852938-0.797615004625-0.7969250380334.076110388462.214775778512.106875272680.103765732720.425707574271-0.422978852456-0.361456939716-0.2565735085160.4136391827070.0872294201357-0.3664779393940.09605078636090.254920989907-0.0524330016279-0.05753170775960.359426040174-0.01063137058980.3474567553674.69639434392-47.418726779352.7810839015
26.030379080242.074875669131.986691804334.382121493690.7849063192014.635235374640.261578679122-0.0802557206023-1.286319610080.2109855777590.0422528774319-1.162779720790.1480005803010.472247423652-0.399597100560.354881932413-0.00827917518775-0.009593232336260.3226091799190.008155880949410.53973249095819.7503867557-56.173959050556.244274797
32.672287429590.144076695074-1.285515966261.98413492087-1.052359231486.465755420390.1024641938430.479059914493-0.688665800096-0.2218697912750.2064785005520.5624590558631.1145034078-0.618248380888-0.3634082745220.398341565225-0.0672280783036-0.05307709479070.260541295383-0.01636190876750.4650970954739.42510922109-55.959052140358.6544094639
44.646228090721.382815816940.4839989010513.968882559941.343939176898.162690651220.0976553946575-0.414838146493-0.1137411269331.51807793076-0.339534224814-0.4591702717661.05653018073-0.1527027633780.2575081709170.468309854692-0.0722108567865-0.0339583593190.2879800543820.1141914707930.4459293797835.8313266897-52.451809930473.758045735
55.80284980846-1.011138749460.06660216448292.879109946680.3073057235932.05223545986-0.325955406489-0.4387510913310.5411513938670.3555462795430.1860473534780.3062981615210.186008117772-0.4246394265450.1174852398530.270002105437-0.005952303803140.008066863152490.3719697651120.0005580385793610.2864284716578.94949125847-42.414713711973.9587267723
61.50478911535-0.0729695072813-0.3531872354481.794765576040.4083176530391.82804876422-0.095611560533-0.117087860164-0.000895017268784-0.109250146740.062003191242-0.1242928505170.1803437599760.2322257242170.0439418847260.2411615972110.00298754642808-0.004113735483030.2682279565550.0160196689930.28846277615915.6075859945-44.928101203467.5784449003
72.09932810284-0.336546889792-0.4505412025322.548872176291.164944721281.919173258620.0690359135994-0.3648881278530.145876889510.2874246249670.0894272457444-0.314782244761-0.03697114563610.459082183199-0.1325004350210.397719556145-0.0183412101894-0.0267886630680.36204480973-0.02139325684480.33767894195618.0989576748-39.524893101478.2505067049
81.537515234780.131869448042-0.7936907509252.579302126481.594281744362.83523968051-0.0249170463227-0.0479193602082-0.3248187824950.541818052609-0.0283713584608-0.1058541492450.390726997680.2845247904580.04105673565490.4804002172130.0423857000061-0.03677678049770.3382010450490.006051877038990.31812842135618.5003403683-49.560576505679.7766682477
92.22806712298-1.620261758361.1867241094.35720633687-1.616785370383.55190009091-0.189814302890.110283994795-0.704131223309-0.1250521407930.4284159519270.8786012333280.881398105347-0.0415421716139-0.1946067395590.820734761968-0.1002431735610.05056398573740.473459423555-0.04948060236780.5948256592993.76628138454-55.51902203371.4313485164
103.967247817120.3104939066740.4168429311543.868329028330.7023676407574.09991685580.289794489011-0.1456330321720.3189805829670.488780243228-0.136709076939-0.0999228508455-0.1390039596460.232302392664-0.2257193725710.342920869205-0.0290323712582-0.01160635178660.2892128864280.02333598885610.26837048417510.5996980561-32.44562852267.7913958915
111.73504218628-0.3034539335450.2563566391282.02349474843-0.9227190883111.87513235167-0.01139997136710.063652011963-0.150557524478-0.1170706631460.01698624725290.05964997582440.04858151785860.0907603579294-0.003923399476410.246290611949-0.0151143038349-0.00344011382030.257288860173-0.001677117429720.24531467806618.3618114456-41.645957401654.8927766966
123.129489147780.9857916289120.2355954548271.60064787577-0.1376170606221.457146033360.126689443503-0.239568707316-0.4982315003280.00832522521054-0.0088083765657-0.1168454940090.1067479638560.0536257106686-0.1125835606260.331613195141-0.0059166588431-0.017304468750.322641934315-0.02147075822320.29633322439531.3044864344-44.954370390550.3749538249
134.32422200654-0.204214933989-2.059003075910.8963731669310.1774472471933.30059194503-0.0513872408448-0.480937081053-0.06017479397310.0885192608162-0.0213773717748-0.30479594711-0.04227358782830.7233199329820.06622830939570.322318370344-0.00135725484197-0.02956026212970.3711868620450.001229349354760.32038428247335.821200061-33.306452710351.4816235622
143.75463787089-0.6789198709-1.082149836653.2808486379-0.5201628407132.827603254210.04551856121060.03126922759850.206457064742-0.25422120495-0.007218288059660.0328197053772-0.3495866680150.0547204050476-0.01692269727490.32006678269-0.0276629583754-0.004286394012440.280698273176-0.002026437837770.21826418674829.8676059191-33.087110212845.0215646885
151.104099575660.0358334319892-0.7546445237941.02977709580.195962309451.95963162393-0.1022063516290.0263464341538-0.259312206011-0.1489344086310.09717754966730.006613844194820.04125009194310.230525708832-0.0701352205080.305981259255-0.0180841494863-0.008302115011780.288898362206-0.02883890158090.29579735375817.3227769285-27.445318758655.4629535955
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634.318098520581.06224664494-0.2884208088915.686446994230.4185927534875.305419261370.5557638450130.04734930299351.003876243680.63067396103-0.566174143383-0.200590371421-0.00801061037381-0.159743491491-0.1357157081420.5059444179940.0803184143660.05038417142190.3198851663930.01874939231060.2662852587497.39949833761-5.6649606079374.5308670299
643.712711980371.574615609071.778610991992.049837719350.6648499358198.76951487646-0.220681049794-0.8215946711640.199429262868-0.2974892135410.781656261462-0.557986530515-1.03373859552-0.641490956042-0.4519428343580.581318917335-0.03818703121610.035395563540.505243000853-0.05628161819720.4068599505697.60169438566-10.3377797477.8362876582
654.9431620737-0.81356211601-0.5386296883655.67647029709-0.5888210046445.80358830727-0.0715820433242-1.685301862050.2114256735650.7870517672660.6421968152530.5901843024050.3742745527310.574050397099-0.5547936287760.5093011128340.05635304160150.1135006906850.50112165755-0.02030068988720.3582046597732.82350171318-15.805319857579.2335752484
663.217591400172.009215214861.554674715934.162894675541.995897128275.435749282660.324361729069-1.04718507962-0.3898736092261.98616333398-0.2356949334131.765616782380.6679067283-0.962066652311-0.0399767883270.516952854411-0.03696296501910.1187028883720.4467847736580.1007377668790.4928813428340.423148162693-21.975902242976.4878727372
675.574878014870.5068478838381.407441875082.7315618457-0.9127020025113.57428940985-0.3814608847510.5251518240820.9025820804150.725157080045-0.236102961841-0.728253362459-0.1021486762190.07502496982350.3572832977520.5994298646210.0411215429147-0.08404419823910.4732559929240.01230538619620.68045381981-1.60432310973-27.445190677971.2395112002
680.852127284078-0.184132565763-0.7441887161681.23329747971-0.4989553085683.4988018255-0.124493562948-0.880597360817-0.01123867300230.6071054823320.2445840067570.353593501620.24150394101-1.05534997975-0.2067603241930.9950014556390.01783888935930.08557354838060.7579596406450.1011791332050.639150089767-6.3358601103-30.918453158869.2270244882
精密化 TLSグループ

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11(chain A and resid 2:16)AA2 - 161 - 15
22(chain A and resid 17:28)AA17 - 2816 - 27
33(chain A and resid 29:38)AA29 - 3828 - 37
44(chain A and resid 39:49)AA39 - 4938 - 48
55(chain A and resid 50:59)AA50 - 5949 - 58
66(chain A and resid 60:86)AA60 - 8659 - 85
77(chain A and resid 87:107)AA87 - 10786 - 106
88(chain A and resid 108:124)AA108 - 124107 - 123
99(chain A and resid 125:135)AA125 - 135124 - 134
1010(chain A and resid 136:161)AA136 - 161135 - 157
1111(chain A and resid 162:213)AA162 - 213158 - 209
1212(chain A and resid 214:230)AA214 - 230210 - 226
1313(chain A and resid 231:245)AA231 - 245227 - 241
1414(chain A and resid 246:278)AA246 - 278242 - 274
1515(chain A and resid 279:292)AA279 - 292275 - 288
1616(chain A and resid 293:300)AA293 - 300289 - 296
1717(chain A and resid 301:314)AA301 - 314297 - 310
1818(chain A and resid 315:324)AA315 - 324311 - 320
1919(chain A and resid 325:374)AA325 - 374321 - 370
2020(chain A and resid 375:389)AA375 - 389371 - 385
2121(chain B and resid 2:14)BE2 - 141 - 13
2222(chain B and resid 15:30)BE15 - 3014 - 29
2323(chain B and resid 31:46)BE31 - 4630 - 45
2424(chain B and resid 47:54)BE47 - 5446 - 53
2525(chain B and resid 55:82)BE55 - 8254 - 81
2626(chain B and resid 83:98)BE83 - 9882 - 97
2727(chain B and resid 99:115)BE99 - 11598 - 114
2828(chain B and resid 116:124)BE116 - 124115 - 123
2929(chain B and resid 128:145)BE128 - 145124 - 141
3030(chain B and resid 146:161)BE146 - 161142 - 154
3131(chain B and resid 162:213)BE162 - 213155 - 206
3232(chain B and resid 214:230)BE214 - 230207 - 223
3333(chain B and resid 231:243)BE231 - 243224 - 236
3434(chain B and resid 244:278)BE244 - 278237 - 271
3535(chain B and resid 279:295)BE279 - 295272 - 288
3636(chain B and resid 296:304)BE296 - 304289 - 297
3737(chain B and resid 305:313)BE305 - 313298 - 306
3838(chain B and resid 314:324)BE314 - 324307 - 317
3939(chain B and resid 325:381)BE325 - 381318 - 374
4040(chain B and resid 382:389)BE382 - 389375 - 382
4141(chain C and resid 3:6)CG3 - 61 - 4
4242(chain C and resid 7:11)CG7 - 115 - 9
4343(chain C and resid 12:15)CG12 - 1510 - 13
4444(chain C and resid 16:19)CG16 - 1914 - 17
4545(chain C and resid 20:23)CG20 - 2318 - 21
4646(chain C and resid 24:29)CG24 - 2922 - 27
4747(chain C and resid 30:34)CG30 - 3428 - 32
4848(chain C and resid 35:38)CG35 - 3833 - 36
4949(chain C and resid 39:42)CG39 - 4237 - 40
5050(chain C and resid 43:46)CG43 - 4641 - 44
5151(chain C and resid 47:50)CG47 - 5045 - 48
5252(chain C and resid 51:54)CG51 - 5449 - 52
5353(chain C and resid 55:58)CG55 - 5853 - 56
5454(chain C and resid 59:62)CG59 - 6257 - 60
5555(chain D and resid 3:6)DH3 - 61 - 4
5656(chain D and resid 7:10)DH7 - 105 - 8
5757(chain D and resid 11:19)DH11 - 199 - 17
5858(chain D and resid 20:23)DH20 - 2318 - 21
5959(chain D and resid 24:29)DH24 - 2922 - 27
6060(chain D and resid 30:33)DH30 - 3328 - 31
6161(chain D and resid 34:37)DH34 - 3732 - 35
6262(chain D and resid 38:41)DH38 - 4136 - 39
6363(chain D and resid 42:45)DH42 - 4540 - 43
6464(chain D and resid 46:50)DH46 - 5044 - 48
6565(chain D and resid 51:54)DH51 - 5449 - 52
6666(chain D and resid 55:58)DH55 - 5853 - 56
6767(chain D and resid 59:62)DH59 - 6257 - 60
6868(chain D and resid 63:66)DH63 - 6661 - 64

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万見について

-
お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る