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- PDB-8ebn: Structure of KLHDC2-EloB/C tetrameric assembly -

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Basic information

Entry
Database: PDB / ID: 8ebn
TitleStructure of KLHDC2-EloB/C tetrameric assembly
Components
  • Elongin-B
  • Elongin-C
  • Kelch domain-containing protein 2
KeywordsLIGASE / E3 ligase
Function / homology
Function and homology information


ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / target-directed miRNA degradation / elongin complex / VCB complex / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation ...ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / target-directed miRNA degradation / elongin complex / VCB complex / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / ubiquitin-like ligase-substrate adaptor activity / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / transcription corepressor binding / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / Vif-mediated degradation of APOBEC3G / Inactivation of CSF3 (G-CSF) signaling / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Evasion by RSV of host interferon responses / Regulation of expression of SLITs and ROBOs / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Neddylation / protein-containing complex assembly / ubiquitin-dependent protein catabolic process / nuclear membrane / protein-macromolecule adaptor activity / proteasome-mediated ubiquitin-dependent protein catabolic process / protein ubiquitination / nuclear body / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytosol
Similarity search - Function
KLHDC2/KLHL20/DRC7 Kelch-repeats domain / Elongin-C / Elongin B / Kelch-type beta propeller / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Ubiquitin family ...KLHDC2/KLHL20/DRC7 Kelch-repeats domain / Elongin-C / Elongin B / Kelch-type beta propeller / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
Elongin-C / Elongin-B / Kelch domain-containing protein 2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsScott, D.C. / Schulman, B.A.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)R01GM125885 United States
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)P30CA021765 United States
CitationJournal: Mol Cell / Year: 2023
Title: E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity.
Authors: Daniel C Scott / Moeko T King / Kheewoong Baek / Clifford T Gee / Ravi Kalathur / Jerry Li / Nicholas Purser / Amanda Nourse / Sergio C Chai / Sivaraja Vaithiyalingam / Taosheng Chen / ...Authors: Daniel C Scott / Moeko T King / Kheewoong Baek / Clifford T Gee / Ravi Kalathur / Jerry Li / Nicholas Purser / Amanda Nourse / Sergio C Chai / Sivaraja Vaithiyalingam / Taosheng Chen / Richard E Lee / Stephen J Elledge / Gary Kleiger / Brenda A Schulman /
Abstract: E3 ligase recruitment of proteins containing terminal destabilizing motifs (degrons) is emerging as a major form of regulation. How those E3s discriminate bona fide substrates from other proteins ...E3 ligase recruitment of proteins containing terminal destabilizing motifs (degrons) is emerging as a major form of regulation. How those E3s discriminate bona fide substrates from other proteins with terminal degron-like sequences remains unclear. Here, we report that human KLHDC2, a CRL2 substrate receptor targeting C-terminal Gly-Gly degrons, is regulated through interconversion between two assemblies. In the self-inactivated homotetramer, KLHDC2's C-terminal Gly-Ser motif mimics a degron and engages the substrate-binding domain of another protomer. True substrates capture the monomeric CRL2, driving E3 activation by neddylation and subsequent substrate ubiquitylation. Non-substrates such as NEDD8 bind KLHDC2 with high affinity, but its slow on rate prevents productive association with CRL2. Without substrate, neddylated CRL2 assemblies are deactivated via distinct mechanisms: the monomer by deneddylation and the tetramer by auto-ubiquitylation. Thus, substrate specificity is amplified by KLHDC2 self-assembly acting like a molecular timer, where only bona fide substrates may bind before E3 ligase inactivation.
History
DepositionAug 31, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 22, 2023Provider: repository / Type: Initial release
Revision 1.1Sep 13, 2023Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 25, 2023Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Kelch domain-containing protein 2
B: Kelch domain-containing protein 2
C: Elongin-B
D: Elongin-C
E: Elongin-B
F: Elongin-C


Theoretical massNumber of molelcules
Total (without water)143,6986
Polymers143,6986
Non-polymers00
Water1,20767
1
A: Kelch domain-containing protein 2
B: Kelch domain-containing protein 2
C: Elongin-B
D: Elongin-C
E: Elongin-B
F: Elongin-C

A: Kelch domain-containing protein 2
B: Kelch domain-containing protein 2
C: Elongin-B
D: Elongin-C
E: Elongin-B
F: Elongin-C


Theoretical massNumber of molelcules
Total (without water)287,39512
Polymers287,39512
Non-polymers00
Water21612
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation4_554-y,-x,-z-1/31
Buried area29390 Å2
ΔGint-192 kcal/mol
Surface area85260 Å2
MethodPISA
Unit cell
Length a, b, c (Å)142.431, 142.431, 140.479
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number151
Space group name H-MP3112

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Components

#1: Protein Kelch domain-containing protein 2 / Hepatocellular carcinoma-associated antigen 33 / Host cell factor homolog LCP / Host cell factor- ...Hepatocellular carcinoma-associated antigen 33 / Host cell factor homolog LCP / Host cell factor-like protein 1 / HCLP-1


Mass: 46154.672 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: KLHDC2, HCA33 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9Y2U9
#2: Protein Elongin-B / EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / ...EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / Transcription elongation factor B polypeptide 2


Mass: 11748.406 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOB, TCEB2 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q15370
#3: Protein Elongin-C / EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / ...EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / Transcription elongation factor B polypeptide 1


Mass: 13945.732 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOC, TCEB1 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q15369
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 67 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.86 Å3/Da / Density % sol: 57.03 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop
Details: % PEG3350, 0.2 M KSCN, 0.1 M Tris, 0.1M Na/K Tartrate, 2.5% MPD, pH =6.8

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 1 Å
DetectorType: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Jul 30, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.6→50 Å / Num. obs: 49565 / % possible obs: 98.6 % / Redundancy: 4 % / Biso Wilson estimate: 59.05 Å2 / Rmerge(I) obs: 0.067 / Rpim(I) all: 0.037 / Rrim(I) all: 0.077 / Χ2: 1.319 / Net I/σ(I): 11.5 / Num. measured all: 197411
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
2.6-2.6940.61349480.8290.3450.7050.50999.6
2.69-2.840.44649490.9040.2510.5130.55899.5
2.8-2.933.90.29949460.9450.170.3450.67598.9
2.93-3.083.70.19548120.9640.1150.2270.79596.2
3.08-3.284.20.14849930.9830.0820.1690.98999.6
3.28-3.534.10.10649870.990.0590.1221.40499.4
3.53-3.8840.07949940.9920.0440.0911.86499.5
3.88-4.453.80.05848820.9940.0340.0682.39696.6
4.45-5.64.10.04650110.9960.0260.0532.24899.1
5.6-5040.0350430.9990.0160.0341.73797.5

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Processing

Software
NameVersionClassification
HKL-2000data scaling
PHENIX1.20.1_4487refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1VCB

1vcb


Resolution: 2.6→46.62 Å / SU ML: 0.4 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 27.37 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2497 2542 5.14 %
Rwork0.2111 46922 -
obs0.2131 49464 98.41 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 177.44 Å2 / Biso mean: 74.7516 Å2 / Biso min: 28.57 Å2
Refinement stepCycle: final / Resolution: 2.6→46.62 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8396 0 0 67 8463
Biso mean---55.66 -
Num. residues----1080
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 18

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.6-2.650.44441550.37262575273099
2.65-2.70.35761270.33572611273899
2.7-2.760.31821320.3092617274999
2.76-2.820.29221480.28122570271899
2.82-2.90.31631340.26932650278499
2.9-2.970.35261460.26532583272997
2.97-3.060.3131250.2592463258894
3.06-3.160.29261240.26012644276899
3.16-3.270.28861290.272426482777100
3.27-3.40.30481420.2612624276699
3.4-3.560.29681310.22322654278599
3.56-3.750.23731440.203926242768100
3.75-3.980.1891610.18692632279399
3.98-4.290.20261760.16892523269998
4.29-4.720.21151420.15172528267095
4.72-5.40.19231300.165426812811100
5.4-6.80.24851370.20762659279699
6.8-46.620.24461590.19142636279596
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.49060.23110.83180.7183-0.27740.9324-0.03220.16640.08330.08540.07660.0478-0.17540.113400.4014-0.0750.09290.22560.01680.423314.9297-32.0701-51.2781
21.6201-0.04770.16260.5723-0.09450.70820.1698-0.0243-0.0471-0.0093-0.0206-0.05580.06990.16110.00010.3605-0.03660.04350.2234-0.01810.379317.175-49.2757-42.9308
30.97830.2557-0.21031.1219-0.04990.9060.1638-0.00830.1085-0.0564-0.0725-0.0808-0.20170.3780.02730.3234-0.11530.07190.3828-0.03760.420634.1361-38.2892-44.939
40.3959-0.34610.32430.2035-0.08780.1286-0.1762-0.4247-0.1988-0.17040.17240.3194-0.3165-0.12600.7077-0.15420.04990.62670.00490.646529.7386-18.9723-69.6946
50.9270.2023-0.40790.7428-0.88840.8497-0.7124-0.03140.1838-0.02270.2608-0.1931-0.4583-0.1336-0.00750.7436-0.1113-0.110.6478-0.16580.649222.2859-23.4449-2.7455
60.13010.1125-0.09430.23780.04240.0824-0.8004-0.25780.94350.27470.4091-0.0852-1.11320.2548-0.00081.18080.0989-0.23991.0179-0.1131.103116.0384-10.6181-0.9817
70.98691.374-0.56742.2059-1.41362.1296-0.136-0.15711.73040.21890.69980.24320.2095-0.08151.49870.67210.4104-0.16111.1363-0.78940.95346.9201-12.65668.6104
80.03120.045-0.05970.0188-0.06770.0770.6897-0.28751.7957-0.3325-0.0849-0.1030.00120.1385-0.00091.10320.21060.05331.0608-0.11291.55215.3376-9.87163.1659
9-0.01760.03-0.03790.5-0.3410.3009-0.5582-0.53880.7913-0.21351.0742-0.2804-0.02490.31170.11740.77720.0509-0.061.2642-0.3590.88020.1229-20.61769.1224
103.37410.94721.39690.36690.32771.3576-0.671-1.21111.32960.68260.15120.3546-0.09390.0622-0.07870.8720.0670.1541.5937-0.38430.68140.0668-25.084112.955
110.1298-0.15550.22411.2944-0.35030.9074-0.0714-1.3490.10220.32040.22060.19160.182-0.33520.55180.5797-0.0597-0.06121.8033-0.3110.04198.3982-33.70210.397
120.1709-0.31650.04092.3009-0.10690.0378-0.1584-0.4842-0.11840.26770.0327-0.62810.2718-0.9143-0.12540.6816-0.2038-0.04331.1764-0.03920.283410.0166-36.54716.7944
130.22280.116-0.19520.5221-0.49380.4405-0.2343-0.0705-0.0535-0.08470.1411-0.2750.52920.2697-0.00380.5856-0.0022-0.03510.778-0.08150.578219.5505-34.47576.562
140.48020.2644-0.19660.1559-0.23660.19440.2646-0.3984-0.41280.1559-0.35310.16090.6472-0.22290.00220.5123-0.125-0.00410.49230.08490.420413.785-53.1783-20.5435
150.1213-0.305-0.04940.5331-0.01920.0296-0.0029-0.14480.48580.02790.30120.3795-0.35290.19930.0050.566-0.11850.06490.47010.01290.4088.3568-40.1447-30.3158
160.2522-0.2209-0.00890.75920.11970.47180.3002-0.8634-0.80810.4897-0.36850.09730.2021-0.1975-0.02570.5539-0.18190.0960.65430.1610.5273-11.2281-61.779-7.0989
170.2347-0.5364-0.21351.43230.87660.83110.1802-0.3476-0.26340.1286-0.19940.977-0.1486-0.6536-0.4930.448-0.19590.13990.61510.24080.754-23.3596-63.6337-8.224
181.5919-0.2326-0.13590.01810.00560.0188-0.11950.2535-0.56330.3723-0.28720.97390.3738-0.532-0.15950.7257-0.22230.30330.59310.29120.9194-16.0609-72.9482-5.3755
190.00740.00610.03080.25360.12960.08730.1209-0.4359-0.82851.28960.2088-0.5354-0.10160.45160.05380.8829-0.1774-0.0170.94060.34030.722-5.0661-64.4659-0.9296
200.9225-0.2056-0.35620.19080.0560.4261-0.2950.2538-0.6351-0.1995-0.2338-0.35310.36110.343-0.72370.5906-0.19510.13440.37340.07010.8607-16.1031-71.2063-14.8734
210.425-0.0640.44430.0190.1173.2271-0.0106-0.1407-0.2708-0.1548-0.17010.44910.1332-0.812-0.25730.765-0.18550.22080.24530.06870.6627-1.1072-65.1062-23.8027
220.3424-0.36890.21310.6498-0.87382.32450.4837-0.4022-0.32790.0743-0.24790.34721.3461-0.46250.25670.4282-0.20270.19660.44620.02490.5579-11.7566-54.8071-20.1325
230.06020.0736-0.05170.12660.03030.03740.3521-0.4919-0.0266-0.0391-0.1580.4008-0.41490.30780.00060.5815-0.10510.13940.5326-0.00580.4642-10.076-47.1777-15.3198
240.23730.13590.06220.1009-0.01480.08930.1119-0.03870.4654-0.1950.1033-0.4330.3587-0.05720.00230.4942-0.05030.07620.32540.03830.4506-9.427-49.5202-25.1592
250.4156-0.50430.15220.4591-0.12390.05260.0507-0.8636-0.42440.37940.05280.39840.29690.37060.02340.5656-0.15530.06850.61580.03070.4055-1.5586-53.1038-11.3505
260.21-0.02120.140.12230.12150.32850.3454-0.52640.0213-0.1645-0.27390.02580.2077-0.6668-0.01840.7598-0.15790.08750.65760.08440.52514.4904-51.0369-3.0474
270.4318-0.1892-0.27320.43510.71761.24920.27070.22210.0642-0.0414-0.7384-0.2426-0.1062-0.0946-0.28810.4498-0.19290.13840.39970.05630.42844.9226-50.1293-20.7071
280.0285-0.01280.05780.09020.19070.6035-0.11550.16880.46260.50410.7362-0.00120.2108-0.30980.01630.6092-0.14340.03080.7659-0.00550.473658.7164-30.4204-70.8783
290.1047-0.07350.10170.0921-0.02290.04390.2358-0.05990.92330.16250.1101-0.23240.347-0.21220.01530.581-0.1585-0.04521.05910.06510.555660.9692-32.5474-74.8717
300.18180.04730.05490.51390.13820.2909-0.27820.2346-0.4705-0.46490.2633-0.2580.0552-0.62010.00390.3976-0.26570.15041.42750.14940.473467.4601-38.4517-76.8755
311.04350.19221.73030.70260.02393.04350.691.59570.2289-0.611-0.1566-1.11090.08532.01920.15010.4663-0.33050.07841.29510.17760.830670.8729-30.7442-78.3122
320.0893-0.1291-0.0820.50070.33540.2585-0.40951.0579-0.0456-0.4465-0.1636-0.2817-0.54840.2905-0.54310.8306-0.57410.19440.87540.0470.656861.3904-27.1141-70.5332
330.5017-0.3399-0.01540.08490.03880.1150.08160.52010.1221-0.0232-0.2085-0.0041-1.04720.2926-0.06340.8151-0.08370.14350.87490.27930.583454.569-21.0841-85.6727
340.3903-0.2169-0.11150.24640.04920.0567-0.24230.8517-0.3676-0.3538-0.0445-0.09270.0690.4748-0.00020.6766-0.16610.09830.8792-0.08480.528248.0348-39.0106-81.203
350.6087-0.2431-0.10080.3096-0.1580.1547-0.14620.14160.17020.0977-0.35910.0078-0.21220.4903-0.03910.6645-0.24620.13360.78940.00470.48945.9146-32.8577-78.0864
360.33640.061-0.1701-0.001-0.02030.0986-0.3292-0.3453-0.0559-0.27630.3864-0.3963-0.21520.27340.01150.7926-0.2110.12280.7460.00480.472444.3084-29.2748-62.5924
370.25-0.09310.00610.06330.04870.05090.2730.2557-0.2945-0.00580.0449-0.21430.0456-0.32440.00030.6646-0.15380.03970.77990.10720.52837.1553-27.4324-79.0231
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 27 through 94 )A27 - 94
2X-RAY DIFFRACTION2chain 'A' and (resid 95 through 221 )A95 - 221
3X-RAY DIFFRACTION3chain 'A' and (resid 222 through 363 )A222 - 363
4X-RAY DIFFRACTION4chain 'A' and (resid 364 through 406 )A364 - 406
5X-RAY DIFFRACTION5chain 'B' and (resid 27 through 106 )B27 - 106
6X-RAY DIFFRACTION6chain 'B' and (resid 107 through 148 )B107 - 148
7X-RAY DIFFRACTION7chain 'B' and (resid 149 through 188 )B149 - 188
8X-RAY DIFFRACTION8chain 'B' and (resid 189 through 207 )B189 - 207
9X-RAY DIFFRACTION9chain 'B' and (resid 208 through 233 )B208 - 233
10X-RAY DIFFRACTION10chain 'B' and (resid 234 through 271 )B234 - 271
11X-RAY DIFFRACTION11chain 'B' and (resid 272 through 296 )B272 - 296
12X-RAY DIFFRACTION12chain 'B' and (resid 297 through 340 )B297 - 340
13X-RAY DIFFRACTION13chain 'B' and (resid 341 through 363 )B341 - 363
14X-RAY DIFFRACTION14chain 'B' and (resid 364 through 387 )B364 - 387
15X-RAY DIFFRACTION15chain 'B' and (resid 388 through 406 )B388 - 406
16X-RAY DIFFRACTION16chain 'C' and (resid 1 through 23 )C1 - 23
17X-RAY DIFFRACTION17chain 'C' and (resid 24 through 41 )C24 - 41
18X-RAY DIFFRACTION18chain 'C' and (resid 42 through 56 )C42 - 56
19X-RAY DIFFRACTION19chain 'C' and (resid 57 through 72 )C57 - 72
20X-RAY DIFFRACTION20chain 'C' and (resid 73 through 90 )C73 - 90
21X-RAY DIFFRACTION21chain 'C' and (resid 91 through 97 )C91 - 97
22X-RAY DIFFRACTION22chain 'D' and (resid 17 through 27 )D17 - 27
23X-RAY DIFFRACTION23chain 'D' and (resid 28 through 46 )D28 - 46
24X-RAY DIFFRACTION24chain 'D' and (resid 47 through 66 )D47 - 66
25X-RAY DIFFRACTION25chain 'D' and (resid 67 through 83 )D67 - 83
26X-RAY DIFFRACTION26chain 'D' and (resid 84 through 96 )D84 - 96
27X-RAY DIFFRACTION27chain 'D' and (resid 97 through 112 )D97 - 112
28X-RAY DIFFRACTION28chain 'E' and (resid 1 through 8 )E1 - 8
29X-RAY DIFFRACTION29chain 'E' and (resid 9 through 23 )E9 - 23
30X-RAY DIFFRACTION30chain 'E' and (resid 24 through 35 )E24 - 35
31X-RAY DIFFRACTION31chain 'E' and (resid 36 through 56 )E36 - 56
32X-RAY DIFFRACTION32chain 'E' and (resid 57 through 79 )E57 - 79
33X-RAY DIFFRACTION33chain 'E' and (resid 80 through 99 )E80 - 99
34X-RAY DIFFRACTION34chain 'F' and (resid 17 through 47 )F17 - 47
35X-RAY DIFFRACTION35chain 'F' and (resid 48 through 83 )F48 - 83
36X-RAY DIFFRACTION36chain 'F' and (resid 84 through 96 )F84 - 96
37X-RAY DIFFRACTION37chain 'F' and (resid 97 through 112 )F97 - 112

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