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- PDB-8cy3: CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA... -

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Basic information

Entry
Database: PDB / ID: 8cy3
TitleCamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Compound 15
Components
  • (DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)- ...) x 2
  • Site-specific DNA-methyltransferase (adenine-specific)
KeywordsDNA BINDING PROTEIN/DNA / DNA Adenine Methylation / PROTEIN-DNA COMPLEX / TRANSFERASE / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / DNA modification / methylation / nucleic acid binding / hydrolase activity
Similarity search - Function
TaqI-like C-terminal specificity domain / TaqI-like C-terminal specificity domain / Type II restriction enzyme and methyltransferase RM.Eco57I-like / Eco57I restriction-modification methylase / : / N-6 Adenine-specific DNA methylases signature. / DNA methylase, N-6 adenine-specific, conserved site / S-adenosyl-L-methionine-dependent methyltransferase superfamily
Similarity search - Domain/homology
: / N-[3-(4-aminophenyl)propyl]adenosine / DNA / DNA (> 10) / site-specific DNA-methyltransferase (adenine-specific)
Similarity search - Component
Biological speciesClostridioides difficile (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.65 Å
AuthorsZhou, J. / Horton, J.R. / Cheng, X.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049245-23 United States
CitationJournal: J.Med.Chem. / Year: 2023
Title: Systematic Design of Adenosine Analogs as Inhibitors of a Clostridioides difficile- Specific DNA Adenine Methyltransferase Required for Normal Sporulation and Persistence.
Authors: Zhou, J. / Horton, J.R. / Menna, M. / Fiorentino, F. / Ren, R. / Yu, D. / Hajian, T. / Vedadi, M. / Mazzoccanti, G. / Ciogli, A. / Weinhold, E. / Huben, M. / Blumenthal, R.M. / Zhang, X. / ...Authors: Zhou, J. / Horton, J.R. / Menna, M. / Fiorentino, F. / Ren, R. / Yu, D. / Hajian, T. / Vedadi, M. / Mazzoccanti, G. / Ciogli, A. / Weinhold, E. / Huben, M. / Blumenthal, R.M. / Zhang, X. / Mai, A. / Rotili, D. / Cheng, X.
History
DepositionMay 22, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 11, 2023Provider: repository / Type: Initial release
Revision 1.1Jan 18, 2023Group: Structure summary / Category: struct / Item: _struct.title
Revision 1.2Jan 25, 2023Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year / _citation_author.identifier_ORCID
Revision 1.3Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Site-specific DNA-methyltransferase (adenine-specific)
B: Site-specific DNA-methyltransferase (adenine-specific)
C: Site-specific DNA-methyltransferase (adenine-specific)
D: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
E: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
F: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
G: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
H: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
I: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)234,24726
Polymers232,3379
Non-polymers1,90917
Water4,864270
1
A: Site-specific DNA-methyltransferase (adenine-specific)
D: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
E: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,0498
Polymers77,4463
Non-polymers6035
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Site-specific DNA-methyltransferase (adenine-specific)
F: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
G: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,27412
Polymers77,4463
Non-polymers8289
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Site-specific DNA-methyltransferase (adenine-specific)
H: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
I: DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)77,9246
Polymers77,4463
Non-polymers4793
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)81.242, 161.235, 229.584
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 1 types, 3 molecules ABC

#1: Protein Site-specific DNA-methyltransferase (adenine-specific)


Mass: 68887.172 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridioides difficile (bacteria)
Gene: BN1095_20232, BN1096_690041, BN1097_700039, E5F26_07025, E5F27_14470, E5F28_16275, E5F29_09620, E5F30_09400, E5F31_14185, E5F32_03810, E5F33_08870, E5F36_00990, E5F37_03060, E5F38_04245, E5F39_ ...Gene: BN1095_20232, BN1096_690041, BN1097_700039, E5F26_07025, E5F27_14470, E5F28_16275, E5F29_09620, E5F30_09400, E5F31_14185, E5F32_03810, E5F33_08870, E5F36_00990, E5F37_03060, E5F38_04245, E5F39_00835, E5F40_02785, E5F41_17405, E5F42_11180, E5F43_12285, E5F44_14865, E5F45_19095
Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: A0A031WG99, site-specific DNA-methyltransferase (adenine-specific)

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DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)- ... , 2 types, 6 molecules DFHEGI

#2: DNA chain DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')


Mass: 4232.795 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Clostridioides difficile (bacteria)
#3: DNA chain DNA (5'-D(*TP*TP*CP*AP*AP*AP*AP*AP*GP*TP*CP*CP*CP*A)-3')


Mass: 4325.825 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Clostridioides difficile (bacteria)

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Non-polymers , 4 types, 287 molecules

#4: Chemical
ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: K
#5: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C2H6O2
#6: Chemical ChemComp-TAI / N-[3-(4-aminophenyl)propyl]adenosine


Mass: 400.432 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C19H24N6O4 / Feature type: SUBJECT OF INVESTIGATION
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 270 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.4 Å3/Da / Density % sol: 63.84 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop
Details: 21-24% PEG 3350, 0.1 M Tris-HCl pH 7-7.5, 0.28M Potassium Citrate
PH range: 7.0-7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 26, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.65→46.9 Å / Num. obs: 86741 / % possible obs: 97.3 % / Redundancy: 11.3 % / Biso Wilson estimate: 62.12 Å2 / CC1/2: 0.993 / Net I/σ(I): 8.6
Reflection shellResolution: 2.65→2.74 Å / Num. unique obs: 7430 / CC1/2: 0.387 / Rpim(I) all: 0.65

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS
Starting model: 7LNJ

7lnj


Resolution: 2.65→46.88 Å / SU ML: 0.3525 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 26.3158
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2249 1986 2.33 %
Rwork0.1925 83267 -
obs0.1932 85253 96.36 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 76.73 Å2
Refinement stepCycle: LAST / Resolution: 2.65→46.88 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13360 1704 122 270 15456
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003115669
X-RAY DIFFRACTIONf_angle_d0.581321476
X-RAY DIFFRACTIONf_chiral_restr0.04332357
X-RAY DIFFRACTIONf_plane_restr0.00362457
X-RAY DIFFRACTIONf_dihedral_angle_d16.50795976
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.65-2.720.36061080.33834636X-RAY DIFFRACTION76.18
2.72-2.790.39131300.33355470X-RAY DIFFRACTION89.14
2.79-2.870.37531350.33085661X-RAY DIFFRACTION93.12
2.87-2.960.35541380.32085769X-RAY DIFFRACTION95.18
2.96-3.070.30471410.28225921X-RAY DIFFRACTION96.65
3.07-3.190.28711450.25126041X-RAY DIFFRACTION99.12
3.19-3.340.3141460.22226153X-RAY DIFFRACTION99.92
3.34-3.510.24991470.20226140X-RAY DIFFRACTION99.92
3.51-3.730.2371460.18536128X-RAY DIFFRACTION99.94
3.73-4.020.20541480.16566174X-RAY DIFFRACTION99.95
4.02-4.430.1791470.1496187X-RAY DIFFRACTION99.86
4.43-5.070.16121490.14136213X-RAY DIFFRACTION99.8
5.07-6.380.18471500.17116271X-RAY DIFFRACTION99.97
6.38-46.880.19351560.17076503X-RAY DIFFRACTION99.63
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.931405749720.371837929785-0.3003581031866.080375392343.476833281238.58137801805-0.0455982877905-0.0526018104657-0.06765926850680.737217976245-0.156611935490.08583794012240.174751369656-0.5500874040720.2177014523390.6228972219870.09139517257350.04637972072940.387104690485-0.01460547168160.417480947987-32.24275229391.146719064335.4468507148
23.074670400481.933300800723.208721234874.173918949843.767079081586.9689777902-0.279615822066-0.3412606507840.5205088085590.0853526409489-0.2290670356910.370176949469-1.39302864843-1.030329456820.593051069620.9412835064990.1978375914580.04894295585850.555648949539-0.03441980232160.53674341897-37.278607290710.993767610532.2284739051
31.72567097512-0.433664110161-0.3410924687063.254012571790.8359923811113.826215533220.04513338980710.08015629084860.179551036314-0.147946990733-0.0327237025078-0.147969139075-0.4962444816770.0908301580894-0.02040903030280.651233312897-0.02755469979670.06025418197860.471687530016-0.05530271455960.372504732192-22.630433797-3.3193127547718.8768097938
40.203322621388-0.670909563194-0.1983481429411.37255526580.9469917468923.59362163895-0.212036067124-0.1360988047220.07572726640880.4397853682880.1111321790390.03267425993130.274840556654-0.1339592014260.1105189582690.622794304625-0.06884720654830.05210927340550.476446692777-0.003360656988560.43932608042-17.9194213115-23.794128631618.7673803634
53.56974224739-1.253460363431.860049861975.79249525906-0.02627966407763.602955853870.1813490348030.846218434344-0.0199945389563-1.682781885450.02144055860470.5629230887240.137149978777-0.116362959858-0.2160520536710.726030833371-0.0195895725714-0.07687072802890.675485991927-0.02945054856970.400270848799-27.4061473463-27.2203102735-16.7125069033
62.44853495335-0.9017521616041.807530111313.97792580148-2.383777761985.69332354013-0.02737759747520.1417415926350.00464166468547-0.18972363628-0.142309753529-0.2418781088650.005864536548250.222264437720.169886004180.40501794161-0.06945980565820.08226321078810.399710525637-0.02604882466230.336165232002-14.3833364647-30.79510509341.29639972749
75.2593280817-1.903999732092.743752010745.609081401310.2898658894526.741119423730.289516409829-0.113396934044-0.5195917817440.1794274203570.00647920377502-0.03233164017061.179728791010.107243068762-0.2833292051040.6345142507870.06430457621420.06676940460850.3480594856590.03968249072540.391631853738-13.7354705772-48.689876968610.0734570631
84.20259143863-2.508815169394.256545394493.28923677251-3.793811172579.027233035460.2003651938060.2365490960830.114867712635-0.247804256887-0.107543953222-0.0419609513842-0.2110625756560.270326200703-0.08940705580970.7460957639670.04751584616230.07967639627420.4711513442070.09342519284270.475363655744-10.80223951067.53906196738-54.2067860853
96.13371427148-0.8307577706076.140151526881.71202195955-1.484089903388.97927661437-0.3083401132630.4838518249540.731158871416-0.140625039565-0.195902785217-0.0853530116964-1.283979330650.2361359213220.4696024332950.840909515970.1140407143950.07897999679490.4883536819320.1205044340520.535262474253-15.732704411319.3006185738-58.7474163864
101.37659677219-0.4581309745790.8256672557162.49597410543-0.785333685332.488532248290.173355918680.0962555853089-0.0197409046199-0.256594869709-0.08181985317020.0595817143620.046059831823-0.0599728970232-0.1189642429240.6169859072850.06946306339220.02472685282610.4415296010650.06870008632360.326257443228-8.7478885674-4.99078545771-43.3301241594
112.03675843739-0.861315765486-0.1986699779543.243025810430.4133941643412.227423668470.0118755800992-0.132675781470.02699867487090.2612814941940.0432683079567-0.1280900939820.1089975847020.144684604823-0.07071704553650.472224614989-0.00533816195839-0.001755525637930.351524375670.07932407248730.29526359637110.1044624495-17.9354072318-19.6221910548
123.91839359998-0.724414901311-0.4866443432235.22198800153.009890466376.76197227448-0.221415796478-0.846552021683-0.06702731281021.10303785719-0.0575794382561-0.05238388776040.479151598149-0.227044978610.2853367482011.214244969540.1251286857580.001100145873310.710760704693-0.00745118576490.5743143917721.427465403651.7250519092-14.5561984401
132.0149228844-0.0954866606088-0.0317012867662.51209568591.705703699243.02986425497-0.0739776378802-0.732758908551-0.008494266341421.15393103731-0.005579231928470.06224603042860.2878960241-0.1094680535120.1245473762691.453767514670.05222089488830.1573076377540.7252697710660.01992699246040.6697388772621.009429354842.5612051338-14.8695211431
140.0719924765380.633751137619-0.4337650071113.28914255730.747377160487.310908993750.0836629903742-0.04810025031810.1059874123370.493669364548-0.3173595864010.438703123726-0.473523993487-0.8029332250170.2424993036570.9774814733820.09597723588940.05038171798410.543852421451-0.01450314187010.60233230431218.566310500647.6287928464-27.2705719222
152.313353835323.550613121981.810409639737.719878115891.251053153591.97507695936-0.02177142051940.02056509603850.418785537623-0.403079397147-0.123495253870.462773801953-0.591089251966-0.1415432605420.1770768392320.958329788490.1713237457250.1152814661180.593549470338-0.0008147758072910.72309234491115.930106836755.9860016877-32.9276097757
166.19362004231-2.7153374564-0.1532331298132.12516438169-0.02453238178771.649618877910.147144822770.3304341302020.263752845275-0.334256102972-0.138802887242-0.0251501740953-0.007043664707670.0315855850238-0.004669825233130.791352884362-0.02107349158270.1001631031130.3517640988910.01497000757260.57424219184924.391697821830.6322725445-52.9719914178
177.134858951912.09078265854-1.150548913353.3974974258-4.881598824967.935489290830.3603594695450.50108824907-1.21666516958-0.545836252726-0.3936068882180.133767801331.536432455940.772685698636-0.08185237860110.9349002719660.245685743118-0.008526377151930.534440728820.04496981170820.82842640155643.145128372813.2411561979-47.0076617268
182.64975368342-1.512909938850.789406191381.15836624881-0.3648446667972.057253043160.1693485108730.0872390397403-0.211938239578-0.615090385268-0.1402796080830.1666576192830.073221459781-0.0182294461613-0.04919688752940.838176660103-0.003029208252390.07475390313790.3821010476550.06275020382340.63225208657919.198423626930.8779923502-53.7957657401
196.23291644223-4.53855785655-0.09888600304334.891082469580.07489399087714.062479810960.3400615306240.5389425152770.375986690382-0.791591570596-0.1472568627640.134504944948-0.482326849758-0.0670036231494-0.1816107783851.15083543360.0643085891476-0.1417860963050.5274584002710.09627963728090.56751133123813.511504321644.1393111777-67.7183091356
200.57181203955-1.6129158705-0.3996361325734.29430768696-0.08612565210856.17559708088-0.2709722893120.0457672145855-0.554513421338-0.0615146526582-0.007426205660940.7216061503340.355070422582-1.100323676580.1832171276860.514427148341-0.142179599709-0.008794567489020.555901623575-0.06892839715810.464474477039-28.4369533555-19.17697304682.08005761116
210.686225021101-0.2540573767891.711506981945.88216998983-0.342370770894.02054779380.056105109127-0.8059753295940.1739536512620.59360789695-0.1308478148131.497374429720.0771244268099-1.21792205280.1278652815830.639385705953-0.03011405418850.2052818241960.859149265325-0.06054732588630.655237439763-32.3879537504-20.3448088834.25077136864
224.655691511621.32768822566-5.844871336594.01495316197-2.861914369167.082834152580.441406911953-0.7152834519530.504323751774-0.15421532655-0.241449321515-0.399057786294-0.8118824103891.02563216141-0.2006347121070.63189426738-0.09624892996150.03999524826870.4808730287630.007700487797670.4111669947685.5953454604-2.50832850485-25.4618415945
231.29883713236-0.7194387223850.979041018182.40883707583-1.333335821281.85812688598-0.1448835251560.1363243277190.245496187153-0.473955383155-0.106346957925-0.66931379792-0.3046600082460.426603619910.2848092660970.74422106038-0.1014501379240.16390005540.3487973075130.02206553976920.5185084083798.57681972267-0.039630363741-28.4071902786
241.73291126487-1.621737056791.962739675712.826311033310.7115373185595.82774646348-0.3326882592850.130516048709-0.0213515588846-0.492533652058-0.0221641209085-1.56982774715-0.4713429485630.789612639660.3219763687040.906168113366-0.06015410678830.2620718354190.5588568096750.1764672172461.0938140993831.694362620232.947669526-41.4821794824
251.78608555021-3.15122520633-0.8664059181376.08640771454-1.069685598118.95627597307-0.0154678218878-0.5672075945771.479353689-0.616068628884-0.19530493705-0.819607673028-0.8979172907111.536156119990.2730970733050.665592010457-0.164930256421-0.02484618087170.5889016204140.01629192856371.1127635063934.848755790836.8768766534-41.1658266092
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 28 through 90 )AA28 - 901 - 63
22chain 'A' and (resid 91 through 130 )AA91 - 13064 - 103
33chain 'A' and (resid 131 through 273 )AA131 - 273104 - 242
44chain 'A' and (resid 274 through 359 )AA274 - 359243 - 328
55chain 'A' and (resid 360 through 428 )AA360 - 428329 - 397
66chain 'A' and (resid 429 through 535 )AA429 - 535398 - 504
77chain 'A' and (resid 536 through 577 )AA536 - 577505 - 546
88chain 'B' and (resid 28 through 66 )BB28 - 661 - 39
99chain 'B' and (resid 67 through 143 )BB67 - 14340 - 116
1010chain 'B' and (resid 144 through 333 )BB144 - 333117 - 306
1111chain 'B' and (resid 334 through 577 )BB334 - 577307 - 550
1212chain 'C' and (resid 29 through 66 )CC29 - 661 - 38
1313chain 'C' and (resid 67 through 246 )CC67 - 24639 - 208
1414chain 'C' and (resid 247 through 273 )CC247 - 273209 - 235
1515chain 'C' and (resid 274 through 311 )CC274 - 311236 - 273
1616chain 'C' and (resid 312 through 399 )CC312 - 399274 - 361
1717chain 'C' and (resid 400 through 428 )CC400 - 428362 - 390
1818chain 'C' and (resid 429 through 535 )CC429 - 535391 - 497
1919chain 'C' and (resid 536 through 577 )CC536 - 577498 - 539
2020chain 'D' and (resid 1 through 14 )DD1 - 14
2121chain 'E' and (resid 1 through 14 )EE1 - 14
2222chain 'F' and (resid 1 through 14 )FF1 - 14
2323chain 'G' and (resid 1 through 14 )GG1 - 14
2424chain 'H' and (resid 1 through 14 )HH1 - 14
2525chain 'I' and (resid 1 through 14 )II1 - 14

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