+Open data
-Basic information
Entry | Database: PDB / ID: 8bgt | ||||||
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Title | O-Methyltransferase Plu4890 in complex with SAM | ||||||
Components | Methyltransferase Plu4890 | ||||||
Keywords | TRANSFERASE / methyltransferase / polyketide / anthraquinone | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Photorhabdus laumondii subsp. laumondii TTO1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.15 Å | ||||||
Authors | Huber, E.M. / Groll, M. | ||||||
Funding support | 1items
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Citation | Journal: Structure / Year: 2023 Title: A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments. Authors: Huber, E.M. / Kreling, L. / Heinrich, A.K. / Dunnebacke, M. / Pothig, A. / Bode, H.B. / Groll, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8bgt.cif.gz | 279.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8bgt.ent.gz | 229.2 KB | Display | PDB format |
PDBx/mmJSON format | 8bgt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8bgt_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 8bgt_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 8bgt_validation.xml.gz | 24.7 KB | Display | |
Data in CIF | 8bgt_validation.cif.gz | 33.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bg/8bgt ftp://data.pdbj.org/pub/pdb/validation_reports/bg/8bgt | HTTPS FTP |
-Related structure data
Related structure data | 8bgxC 8bgyC 8bgzC 8bh0C 8bibC 8bicC 8bidC 8bieC 8bifC 8bigC 8bihC 8biiC 8bijC 8birC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 36550.527 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Photorhabdus laumondii subsp. laumondii TTO1 (bacteria) Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A6L9JR93 #2: Chemical | #3: Chemical | ChemComp-TRP / | #4: Chemical | ChemComp-GOL / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.93 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.2 M NaF, 0.1 M bis-tris propane pH 7.5, 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 4, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→48 Å / Num. obs: 42753 / % possible obs: 97.4 % / Redundancy: 2.9 % / Rmerge(I) obs: 0.044 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 2.15→2.25 Å / Rmerge(I) obs: 0.517 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 5475 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.15→30 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.949 / SU B: 30.255 / SU ML: 0.301 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.212 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 147.76 Å2 / Biso mean: 73.39 Å2 / Biso min: 42.92 Å2
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Refinement step | Cycle: final / Resolution: 2.15→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.206 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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