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- PDB-8bec: Crystal structure of the SARS-CoV-2 S RBD in complex with pT1375 scFV -

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Basic information

Entry
Database: PDB / ID: 8bec
TitleCrystal structure of the SARS-CoV-2 S RBD in complex with pT1375 scFV
Components
  • Spike protein S1
  • pT1375 single-chain Fv
KeywordsPROTEIN BINDING / PROTEIN BINDING/IMMUNE SYSTEM
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / membrane fusion / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.7 Å
AuthorsHansen, G. / Ssebyatika, G.L. / Krey, T.
Funding support Germany, 6items
OrganizationGrant numberCountry
Other government14-76103-184 CORONA-12/20
Other government14-76103-184 CORONA-15/20 Germany
German Federal Ministry for Education and ResearchCOVIM (FKZ: 01KX2021) Germany
German Federal Ministry for Education and ResearchRAPID (FKZ: 01KI1723G) Germany
German Research Foundation (DFG)EXC 2155 Germany
German Research Foundation (DFG)2485 VIPER (398066876/GRK 2485/1) Germany
CitationJournal: To be published
Title: Activity of broadly neutralizing antibodies against sarbecoviruses: a trade-off between SARS-CoV-2 variants and distant coronaviruses?
Authors: Stein, C.S. / Hansen, G. / Ssebyatika, G.L. / Stroeh, L. / Benecke, T. / Menz, S. / Waldmann, J.-Y. / Vollmer, B. / Tipp, S. / Ochulor, O. / Herold, E. / Schwarzloh, B. / Mutschall, D. / ...Authors: Stein, C.S. / Hansen, G. / Ssebyatika, G.L. / Stroeh, L. / Benecke, T. / Menz, S. / Waldmann, J.-Y. / Vollmer, B. / Tipp, S. / Ochulor, O. / Herold, E. / Schwarzloh, B. / Mutschall, D. / Zischke, J.-Y. / Cordes, A. / Puppe, W. / Schneider, T. / Hinrichs, I. / Blasczyk, R. / Kleine-Weber, H. / Hoffmann, M. / Hoeper, M. / Kaiser, F.K. / Gonzalez-Hernandez, M. / Armando, F.K. / Ciurkiewicz, M. / Beythien, G. / Poehlmann, S. / Baumgaertner, W. / Gruenewald, K. / Osterhaus, A. / Schulz, T. / Krey, T.
History
DepositionOct 21, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 1, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: pT1375 single-chain Fv
B: Spike protein S1
C: pT1375 single-chain Fv
D: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)99,4147
Polymers97,5124
Non-polymers1,9023
Water8,989499
1
A: pT1375 single-chain Fv
B: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,5053
Polymers48,7562
Non-polymers7491
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3150 Å2
ΔGint9 kcal/mol
Surface area18600 Å2
MethodPISA
2
C: pT1375 single-chain Fv
D: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,9094
Polymers48,7562
Non-polymers1,1532
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3710 Å2
ΔGint11 kcal/mol
Surface area19050 Å2
MethodPISA
Unit cell
Length a, b, c (Å)87.446, 91.559, 156.704
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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Antibody / Protein , 2 types, 4 molecules ACBD

#1: Antibody pT1375 single-chain Fv / pT1375 scFV


Mass: 26394.203 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): S2 / Production host: Drosophila melanogaster (fruit fly)
#2: Protein Spike protein S1


Mass: 22361.924 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Cell line (production host): S2 / Production host: Drosophila melanogaster (fruit fly) / References: UniProt: P0DTC2

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Sugars , 2 types, 2 molecules

#3: Polysaccharide alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 748.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-6DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3/a4-b1_b4-c1_c6-d1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#4: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1056.964 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5][a1221m-1a_1-5]/1-1-2-3-3-4/a4-b1_a6-f1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE

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Non-polymers , 2 types, 500 molecules

#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 499 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.22 Å3/Da / Density % sol: 61.76 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: pT1375-RBD at 15,5 mg/ml using 1.5 M Ammonium sulfate, 15 % w/v glycerol, 100 mM Tris pH 8.5 as reservoir and micro seeds in 18% PEG 3350, 100 mM citrate pH 4, 200 mM Na3Cit stabilization solution

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 4, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.7→45.78 Å / Num. obs: 138573 / % possible obs: 99.96 % / Redundancy: 13.3 % / Biso Wilson estimate: 29.26 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.07894 / Rpim(I) all: 0.0225 / Rrim(I) all: 0.08214 / Net I/σ(I): 17.68
Reflection shellResolution: 1.7→1.761 Å / Redundancy: 13.1 % / Rmerge(I) obs: 1.532 / Mean I/σ(I) obs: 1.28 / Num. unique obs: 13750 / CC1/2: 0.729 / CC star: 0.918 / Rpim(I) all: 0.4355 / Rrim(I) all: 1.594 / % possible all: 99.98

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation3.89 Å49.21 Å
Translation3.89 Å49.21 Å

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Processing

Software
NameVersionClassification
XDSdata reduction
PHASER2.8.3phasing
PHENIX1.2refinement
PDB_EXTRACT3.27data extraction
XDSdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7KGJ, 7S4S
Resolution: 1.7→45.78 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 22.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2068 1677 1.21 %
Rwork0.1854 136864 -
obs0.1857 138541 99.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 77.89 Å2 / Biso mean: 34.3085 Å2 / Biso min: 20.73 Å2
Refinement stepCycle: final / Resolution: 1.7→45.78 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6496 0 126 499 7121
Biso mean--48.54 38.35 -
Num. residues----838
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 12 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
1.7-1.750.37571380.37011131311451
1.75-1.810.33871340.28861127011404
1.81-1.870.22851430.23311130711450
1.87-1.950.2791380.22031130111439
1.95-2.030.21521430.19561134911492
2.03-2.140.26111360.20671131311449
2.14-2.280.19681320.18591132311455
2.28-2.450.22031460.18831136611512
2.45-2.70.2211360.19461142811564
2.7-3.090.23091460.18171145611602
3.09-3.890.17091390.16471152611665
3.89-45.780.17731460.16841191212058
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
12.3119-0.63841.06753.511-2.18635.01950.0743-0.0844-0.3129-0.0719-0.00510.35930.6483-0.1943-0.12350.2715-0.0360.0310.1711-0.02130.2372-5.883437.272981.3734
20.4829-0.6788-0.09633.6275-0.74413.4148-0.0447-0.016-0.17680.15450.0284-0.07370.35060.08220.01270.222-0.00940.01920.1917-0.0010.2565-0.12939.287478.8719
32.76225.0842-0.03492.86810.19921.5795-0.37720.0407-0.1358-0.27920.3468-0.3280.61690.2252-0.1240.34940.05840.01580.3068-0.01260.42466.071542.921687.9656
43.2633-1.23540.37684.81442.31972.5458-0.0441-0.14670.09350.00150.2855-0.2479-0.24560.4075-0.28820.1768-0.0067-0.00850.2081-0.00260.22969.044660.831893.4566
56.01750.8775-1.0643.4243-3.20069.88060.0581-0.26210.0581-0.09850.09470.25310.4105-0.3367-0.24030.2243-0.00320.02080.1513-0.03640.2021-0.817854.484187.6362
62.5299-1.92291.64547.40291.37177.6415-0.1158-0.15820.3450.1402-0.00470.0309-0.5713-0.15920.11840.1907-0.0133-0.00940.1807-0.01390.22740.675461.811490.1807
74.3118-2.5758-1.88648.69674.81512.2252-0.2083-0.286-0.18370.73560.11180.07320.91330.47310.03220.1268-0.03730.00510.16230.01960.25116.616752.558889.9326
87.62721.0672-7.09464.76810.62358.45830.28130.21540.3224-0.5913-0.1119-0.1099-1.074-0.2134-0.23090.34550.0335-0.03490.22750.03050.27112.580663.258545.6319
92.8176-0.6155-6.68051.84490.35135.86360.23240.27880.1106-0.4021-0.08650.0067-0.3132-0.2613-0.23040.26490.0356-0.02090.39160.00570.21088.052556.609543.1583
101.40170.94991.44012.41440.72984.5107-0.07760.1721-0.1096-0.21090.0373-0.29550.06530.42790.05260.20520.03160.01890.2485-0.00470.235121.336250.180745.0974
114.55510.9509-1.22784.5953-1.6962.41720.0282-0.1117-0.07880.0993-0.14090.03920.13440.03690.12190.13460.01580.00820.1717-0.03450.1517.743250.302257.524
122.81630.7893-0.40754.07722.19674.1967-0.09380.0713-0.0797-0.17370.0999-0.16760.06210.1401-0.01610.20960.0343-0.00820.23730.02410.139511.685452.816754.4785
136.88724.7438-0.2685.6723-0.63671.54730.1129-0.31840.76080.0809-0.17540.5749-0.2817-0.08490.10560.19680.06-0.00370.2554-0.0410.25120.686560.310363.8693
144.249-2.2059-4.10622.70932.04337.312-0.06790.4423-0.1481-0.1019-0.04440.51070.0539-0.77650.11230.1902-0.0235-0.04790.27230.01930.3-10.106353.820463.487
158.5841.28463.6896.1025-3.64014.78150.0733-0.84560.65540.6787-0.10480.2666-0.2422-0.06850.07080.2746-0.0350.04130.3468-0.0810.275311.929262.458668.7304
163.8905-3.1094-3.38614.1463.5513.35050.09180.13-0.0053-0.2844-0.3272-0.01470.0424-0.47820.20490.2749-0.005-0.01320.24480.02220.204612.451254.170750.5877
178.09177.09716.00639.5344.3436.6332-0.49470.5290.1756-0.60150.4260.0407-0.05320.27520.06660.29130.06150.00720.3276-0.01840.194812.678547.844731.7224
186.33752.75231.45158.05333.00724.34-0.00230.228-0.4913-0.49390.0833-0.44940.35450.1957-0.1050.43580.1025-0.01170.23730.02010.265544.630929.88568.0314
190.83630.5633-0.1433.45590.43032.6925-0.026-0.0217-0.2002-0.03640.0561-0.08140.38510.092-0.0410.2740.0365-0.00610.20020.00290.240541.136137.764874.5238
202.632-3.41970.59666.0407-1.29611.595-0.1062-0.039-0.2524-0.10080.13460.41190.4348-0.2245-0.04290.3134-0.0599-0.02490.25580.01070.253233.369439.970664.9861
217.0968-2.0726-3.99152.67361.04458.94060.07190.11950.0455-0.03640.06980.0480.083-0.3097-0.14670.2325-0.0223-0.0630.20730.04810.248431.691656.799556.2911
227.78810.1004-0.13518.28413.2478.07860.14640.4021-0.0452-0.3286-0.1165-0.419-0.16410.1435-0.10950.21340.00830.02080.16580.03540.158441.041750.745162.9213
234.61552.4635-0.04276.7312-1.19164.1190.01440.10820.1878-0.2676-0.0136-0.2151-0.29820.1157-0.03020.23830.0153-0.01050.21020.00340.193140.321357.518759.0917
244.94721.6229-3.45195.3364-4.35626.1099-0.15820.2138-0.1717-0.45340.1034-0.00331.1694-0.4360.09060.17230.0374-0.01250.1378-0.01660.237233.309548.694862.6233
252.1311-0.2777-1.72212.3211-1.0494.15420.053-0.39550.29040.49110.151-0.0583-0.45140.1774-0.20510.2651-0.0008-0.04430.2338-0.05790.226830.728865.0136104.1545
261.2623-0.04-0.53891.0285-0.15211.5558-0.0713-0.0427-0.04280.12370.00030.07660.0279-0.15020.07110.1969-0.0008-0.02670.1925-0.00440.167826.023854.226799.8437
273.07031.0015-1.20664.4746-2.02665.2539-0.0895-0.0854-0.03510.13290.16350.21180.0601-0.263-0.08130.1661-0.0182-0.02980.1689-0.0510.107828.876755.823595.7974
286.5698-2.63240.09496.10551.58641.1622-0.11460.68410.5756-0.3940.1051-0.2275-0.15370.10760.05770.2181-0.0929-0.02140.32340.04190.207739.360761.043384.8285
292.835-0.825-3.07782.15011.39925.2460.1466-0.63820.17970.11310.097-0.6418-0.28191.0297-0.28280.27660.002-0.06090.4115-0.04840.387951.379253.111187.6693
308.9063-1.3891-0.40711.98960.69490.5549-0.0370.74990.2963-0.24640.0387-0.1752-0.170.0789-0.02280.2382-0.03740.00940.31970.02130.191635.928260.889580.829
310.6247-0.60230.77742.1722-3.6026.96410.0273-0.1580.01450.13070.07670.0590.1464-0.0611-0.07560.2326-0.0115-0.03430.2018-0.00520.202228.973357.3635109.0416
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 39 )A2 - 39
2X-RAY DIFFRACTION2chain 'A' and (resid 40 through 108 )A40 - 108
3X-RAY DIFFRACTION3chain 'A' and (resid 109 through 145 )A109 - 145
4X-RAY DIFFRACTION4chain 'A' and (resid 146 through 168 )A146 - 168
5X-RAY DIFFRACTION5chain 'A' and (resid 169 through 187 )A169 - 187
6X-RAY DIFFRACTION6chain 'A' and (resid 188 through 229 )A188 - 229
7X-RAY DIFFRACTION7chain 'A' and (resid 230 through 246 )A230 - 246
8X-RAY DIFFRACTION8chain 'B' and (resid 334 through 349 )B334 - 349
9X-RAY DIFFRACTION9chain 'B' and (resid 350 through 364 )B350 - 364
10X-RAY DIFFRACTION10chain 'B' and (resid 365 through 393 )B365 - 393
11X-RAY DIFFRACTION11chain 'B' and (resid 394 through 421 )B394 - 421
12X-RAY DIFFRACTION12chain 'B' and (resid 422 through 442 )B422 - 442
13X-RAY DIFFRACTION13chain 'B' and (resid 443 through 459 )B443 - 459
14X-RAY DIFFRACTION14chain 'B' and (resid 460 through 494 )B460 - 494
15X-RAY DIFFRACTION15chain 'B' and (resid 495 through 506 )B495 - 506
16X-RAY DIFFRACTION16chain 'B' and (resid 507 through 516 )B507 - 516
17X-RAY DIFFRACTION17chain 'B' and (resid 517 through 527 )B517 - 527
18X-RAY DIFFRACTION18chain 'C' and (resid 1 through 17 )C1 - 17
19X-RAY DIFFRACTION19chain 'C' and (resid 18 through 108 )C18 - 108
20X-RAY DIFFRACTION20chain 'C' and (resid 109 through 145 )C109 - 145
21X-RAY DIFFRACTION21chain 'C' and (resid 146 through 168 )C146 - 168
22X-RAY DIFFRACTION22chain 'C' and (resid 169 through 187 )C169 - 187
23X-RAY DIFFRACTION23chain 'C' and (resid 188 through 229 )C188 - 229
24X-RAY DIFFRACTION24chain 'C' and (resid 230 through 245 )C230 - 245
25X-RAY DIFFRACTION25chain 'D' and (resid 334 through 364 )D334 - 364
26X-RAY DIFFRACTION26chain 'D' and (resid 365 through 421 )D365 - 421
27X-RAY DIFFRACTION27chain 'D' and (resid 422 through 442 )D422 - 442
28X-RAY DIFFRACTION28chain 'D' and (resid 443 through 459 )D443 - 459
29X-RAY DIFFRACTION29chain 'D' and (resid 460 through 487 )D460 - 487
30X-RAY DIFFRACTION30chain 'D' and (resid 488 through 506 )D488 - 506
31X-RAY DIFFRACTION31chain 'D' and (resid 507 through 527 )D507 - 527

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