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- PDB-8bbj: Secretagogin (mouse) in complex with its target peptide from Synt... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8bbj | ||||||
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Title | Secretagogin (mouse) in complex with its target peptide from Syntaxin-4 | ||||||
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![]() | PEPTIDE BINDING PROTEIN / Calcium dependent / protein complex | ||||||
Function / homology | ![]() sphingomyelin phosphodiesterase activator activity / Disinhibition of SNARE formation / positive regulation of eosinophil degranulation / trans-Golgi Network Vesicle Budding / Other interleukin signaling / cornified envelope assembly / neuron projection membrane / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / vesicle-mediated transport in synapse / synaptic vesicle fusion to presynaptic active zone membrane ...sphingomyelin phosphodiesterase activator activity / Disinhibition of SNARE formation / positive regulation of eosinophil degranulation / trans-Golgi Network Vesicle Budding / Other interleukin signaling / cornified envelope assembly / neuron projection membrane / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / vesicle-mediated transport in synapse / synaptic vesicle fusion to presynaptic active zone membrane / synaptic vesicle docking / storage vacuole / lateral loop / regulation of presynaptic cytosolic calcium ion concentration / vesicle fusion / myelin sheath adaxonal region / vesicle docking / SNARE complex / SNAP receptor activity / specific granule / positive regulation of chemotaxis / positive regulation of protein localization to cell surface / regulation of extrinsic apoptotic signaling pathway via death domain receptors / protein localization to cell surface / regulation of long-term synaptic potentiation / SNARE complex assembly / ER-Phagosome pathway / positive regulation of immunoglobulin production / transport vesicle membrane / exocytosis / synaptic vesicle exocytosis / positive regulation of insulin secretion involved in cellular response to glucose stimulus / positive regulation of cell adhesion / endomembrane system / presynaptic active zone membrane / phagocytic vesicle / bioluminescence / SNARE binding / generation of precursor metabolites and energy / long-term synaptic potentiation / positive regulation of protein localization to plasma membrane / intracellular protein transport / Schaffer collateral - CA1 synapse / trans-Golgi network / terminal bouton / sarcolemma / small GTPase binding / cellular response to type II interferon / synaptic vesicle / lamellipodium / cellular response to oxidative stress / postsynapse / protein-containing complex assembly / basolateral plasma membrane / postsynaptic membrane / dendritic spine / membrane fusion / endosome / positive regulation of cell migration / apical plasma membrane / glutamatergic synapse / synapse / calcium ion binding / dendrite / positive regulation of cell population proliferation / perinuclear region of cytoplasm / cell surface / extracellular space / extracellular region / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schnell, R. / Szodorai, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A hydrophobic groove in secretagogin allows for alternate interactions with SNAP-25 and syntaxin-4 in endocrine tissues. Authors: Szodorai, E. / Hevesi, Z. / Wagner, L. / Hokfelt, T.G.M. / Harkany, T. / Schnell, R. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 186.2 KB | Display | ![]() |
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PDB format | ![]() | 145.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 477.2 KB | Display | ![]() |
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Full document | ![]() | 488.6 KB | Display | |
Data in XML | ![]() | 32.8 KB | Display | |
Data in CIF | ![]() | 43.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8banSC ![]() 8bavC ![]() 1emaS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29433.234 Da / Num. of mol.: 2 / Mutation: F64L,Q80R,I167T Source method: isolated from a genetically manipulated source Details: GFP-fusion protein carrying a short peptide from mouse Syntaxin-4 (sequence: GLQNLREEIKQLGREVRAQLKAIEPQKE) on its C-terminus. The Chromophore of GFP is formed from residues Ser65-Tyr66- ...Details: GFP-fusion protein carrying a short peptide from mouse Syntaxin-4 (sequence: GLQNLREEIKQLGREVRAQLKAIEPQKE) on its C-terminus. The Chromophore of GFP is formed from residues Ser65-Tyr66-Gly67, and represented as non-peptide residue CRO (Chromophore / Fluorophore). This alteration results in an apparent mismatch when comparing the encoded polypeptide sequence (present in databases) and the residues present in the PDB file. Source: (gene. exp.) ![]() ![]() ![]() ![]() Gene: GFP, Stx4, Stx4a / Production host: ![]() ![]() #2: Protein | Mass: 21998.104 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | ChemComp-CA / #4: Chemical | ChemComp-CAC / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.55 Å3/Da / Density % sol: 72 % / Description: rod |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 8 Details: 0.1 M Na cacodylate pH 5.5, 0.2 M NH4SO4, 25% v/v PEGSM (PEG-smear) |
-Data collection
Diffraction | Mean temperature: 110 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 11, 2021 / Details: mirrors |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96863 Å / Relative weight: 1 |
Reflection | Resolution: 2.65→78.68 Å / Num. obs: 28954 / % possible obs: 98.2 % / Redundancy: 3.3 % / Biso Wilson estimate: 60.4 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.059 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 2.65→2.78 Å / Rmerge(I) obs: 0.678 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 3847 / CC1/2: 0.598 / Rpim(I) all: 0.574 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1EMA, 8BAN Resolution: 2.65→48.289 Å / SU ML: 0.35 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 31 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.65→48.289 Å
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Refine LS restraints |
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LS refinement shell |
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