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Open data
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Basic information
Entry | Database: PDB / ID: 7z1s | ||||||
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Title | X-ray crystal structure of SLPYL1-NIO complex | ||||||
![]() | SlPYL1-NIO | ||||||
![]() | PLANT PROTEIN / SLPYL1 / ABA receptor protein | ||||||
Function / homology | ![]() abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / signaling receptor activity / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Infantes, L. / Albert, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure-Based Modulation of the Ligand Sensitivity of a Tomato Dimeric Abscisic Acid Receptor Through a Glu to Asp Mutation in the Latch Loop. Authors: Infantes, L. / Rivera-Moreno, M. / Daniel-Mozo, M. / Benavente, J.L. / Ocana-Cuesta, J. / Coego, A. / Lozano-Juste, J. / Rodriguez, P.L. / Albert, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.9 KB | Display | ![]() |
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PDB format | ![]() | 74.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 743.2 KB | Display | ![]() |
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Full document | ![]() | 748.4 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 14.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7z1pC ![]() 7z1qC ![]() 7z1rC ![]() 5moaS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25738.443 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-NIO / |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.77 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: Ammonium sulfate 1.6M |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 19, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97926 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→44.83 Å / Num. obs: 29543 / % possible obs: 99.6 % / Redundancy: 14 % / Biso Wilson estimate: 36.96 Å2 / CC1/2: 1 / CC star: 1 / Rmerge(I) obs: 0.04638 / Rpim(I) all: 0.01248 / Rrim(I) all: 0.04808 / Net I/σ(I): 27.19 |
Reflection shell | Resolution: 1.65→1.709 Å / Redundancy: 13.8 % / Rmerge(I) obs: 3.333 / Mean I/σ(I) obs: 0.68 / Num. unique obs: 2883 / CC1/2: 0.515 / CC star: 0.825 / Rpim(I) all: 0.9231 / Rrim(I) all: 3.461 / % possible all: 96.92 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5MOA Resolution: 1.65→44.83 Å / SU ML: 0.3481 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 37.695 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.28 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→44.83 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -0.798669606701 Å / Origin y: -28.5860661118 Å / Origin z: -4.51856503006 Å
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Refinement TLS group | Selection details: all |