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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7xuw | ||||||
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タイトル | Crystal structure of RPA70N-BLMp2 fusion | ||||||
![]() | fusion protein of Replication protein A 70 kDa DNA-binding subunit and Bloom syndrome protein | ||||||
![]() | DNA BINDING PROTEIN / RPA / RPA70N / BLM | ||||||
機能・相同性 | ![]() regulation of DNA-templated DNA replication / RecQ family helicase-topoisomerase III complex / telomeric G-quadruplex DNA binding / forked DNA-dependent helicase activity / protein localization to chromosome / resolution of DNA recombination intermediates / 8-hydroxy-2'-deoxyguanosine DNA binding / DNA/DNA annealing activity / telomeric D-loop binding / telomere maintenance via semi-conservative replication ...regulation of DNA-templated DNA replication / RecQ family helicase-topoisomerase III complex / telomeric G-quadruplex DNA binding / forked DNA-dependent helicase activity / protein localization to chromosome / resolution of DNA recombination intermediates / 8-hydroxy-2'-deoxyguanosine DNA binding / DNA/DNA annealing activity / telomeric D-loop binding / telomere maintenance via semi-conservative replication / DNA replication factor A complex / cellular response to camptothecin / t-circle formation / G-quadruplex DNA unwinding / telomeric D-loop disassembly / Y-form DNA binding / chromatin-protein adaptor activity / negative regulation of cell division / four-way junction helicase activity / G-quadruplex DNA binding / DNA double-strand break processing / cellular response to hydroxyurea / lateral element / negative regulation of DNA recombination / bubble DNA binding / Removal of the Flap Intermediate / single-stranded telomeric DNA binding / Processive synthesis on the C-strand of the telomere / protein localization to site of double-strand break / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Impaired BRCA2 binding to PALB2 / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Removal of the Flap Intermediate from the C-strand / G-rich strand telomeric DNA binding / DNA 3'-5' helicase / replisome / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / DNA duplex unwinding / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / regulation of cyclin-dependent protein serine/threonine kinase activity / nuclear chromosome / replication fork processing / DNA unwinding involved in DNA replication / mitotic G2 DNA damage checkpoint signaling / 3'-5' DNA helicase activity / site of DNA damage / Presynaptic phase of homologous DNA pairing and strand exchange / telomere maintenance via telomerase / response to X-ray / Activation of the pre-replicative complex / PCNA-Dependent Long Patch Base Excision Repair / Regulation of HSF1-mediated heat shock response / HSF1 activation / ATP-dependent activity, acting on DNA / mismatch repair / Activation of ATR in response to replication stress / SUMOylation of DNA damage response and repair proteins / four-way junction DNA binding / DNA helicase activity / isomerase activity / telomere maintenance / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / helicase activity / meiotic cell cycle / replication fork / molecular function activator activity / cellular response to ionizing radiation / nucleotide-excision repair / Fanconi Anemia Pathway / Recognition of DNA damage by PCNA-containing replication complex / Termination of translesion DNA synthesis / Translesion Synthesis by POLH / double-strand break repair via homologous recombination / base-excision repair / HDR through Homologous Recombination (HRR) / G2/M DNA damage checkpoint / protein homooligomerization / Dual Incision in GG-NER / DNA-templated DNA replication / PML body / Meiotic recombination / nuclear matrix / Formation of Incision Complex in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / p53 binding / protein complex oligomerization / chromosome / site of double-strand break / single-stranded DNA binding / Processing of DNA double-strand break ends 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Wu, Y.Y. / Zang, N. / Fu, W.M. / Zhou, C. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural characterization of human RPA70N association with DNA damage response proteins. 著者: Wu, Y. / Fu, W. / Zang, N. / Zhou, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 70 KB | 表示 | ![]() |
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PDB形式 | ![]() | 50.6 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 424.7 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 425.4 KB | 表示 | |
XML形式データ | ![]() | 8.1 KB | 表示 | |
CIF形式データ | ![]() | 10.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7xutC ![]() 7xuvC ![]() 7xv0C ![]() 7xv1C ![]() 7xv4C ![]() 8jzvC ![]() 8jzyC ![]() 8k00C ![]() 5eayS C: 同じ文献を引用 ( S: 精密化の開始モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 16156.204 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: fusion protein(3-121:RPA70N(P27694), 122-125:linker, 550-564:BLMp2(P54132)) 由来: (組換発現) ![]() ![]() ![]() |
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#2: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.1 Å3/Da / 溶媒含有率: 41.39 % |
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結晶化 | 温度: 278 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 / 詳細: 8% PEG 4000 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2019年6月29日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97918 Å / 相対比: 1 |
反射 | 解像度: 1.8→27.94 Å / Num. obs: 24353 / % possible obs: 99.92 % / 冗長度: 12.3 % / CC1/2: 0.996 / Rmerge(I) obs: 0.1312 / Rpim(I) all: 0.03932 / Net I/σ(I): 13.6 |
反射 シェル | 解像度: 1.8→1.88 Å / Rmerge(I) obs: 0.7671 / Num. unique obs: 2918 / CC1/2: 0.954 / Rpim(I) all: 0.2215 / % possible all: 100 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 5EAY 解像度: 1.8→27.94 Å / SU ML: 0.11 / 交差検証法: THROUGHOUT / σ(F): 1.36 / 位相誤差: 24.83 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso max: 55.93 Å2 / Biso mean: 26.7718 Å2 / Biso min: 11.48 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: final / 解像度: 1.8→27.94 Å
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 8 / % reflection obs: 100 %
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION
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精密化 TLSグループ |
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