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- PDB-7w7b: Heme exporter HrtBA in complex with protoporphyrin IX containing ... -

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Basic information

Entry
Database: PDB / ID: 7w7b
TitleHeme exporter HrtBA in complex with protoporphyrin IX containing manganese(III), high resolution data
Components(Putative ABC transport ...) x 2
KeywordsMEMBRANE PROTEIN / Heme efflux / Heme extraction / Heme detoxification / ABC transporter / 4-Helix TMD / ATP-BINDING PROTEIN-MEMBRANE PROTEIN complex
Function / homology
Function and homology information


transmembrane transporter activity / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane
Similarity search - Function
MacB-like periplasmic core domain / MacB-like periplasmic core domain / MacB, ATP-binding domain / ABC transporter, lipoprotein release, LolD / ABC3 transporter permease protein domain / FtsX-like permease family / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain ...MacB-like periplasmic core domain / MacB-like periplasmic core domain / MacB, ATP-binding domain / ABC transporter, lipoprotein release, LolD / ABC3 transporter permease protein domain / FtsX-like permease family / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Chem-AGA / PROTOPORPHYRIN IX CONTAINING MN / DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / ABC transport system integral membrane protein / ABC transport system, ATP-binding protein
Similarity search - Component
Biological speciesCorynebacterium diphtheriae NCTC 13129 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å
AuthorsHisano, T. / Nakamura, H. / Rahman, M.M. / Tosha, T. / Shirouzu, M. / Shiro, Y.
Funding support Japan, 4items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)16K07309 Japan
Japan Society for the Promotion of Science (JSPS)26220807 Japan
Japan Society for the Promotion of Science (JSPS)JP19H00926 Japan
Japan Society for the Promotion of Science (JSPS)JP19H05761 Japan
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2022
Title: Structural basis for heme detoxification by an ATP-binding cassette-type efflux pump in gram-positive pathogenic bacteria.
Authors: Nakamura, H. / Hisano, T. / Rahman, M.M. / Tosha, T. / Shirouzu, M. / Shiro, Y.
History
DepositionDec 4, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 22, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 11, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Putative ABC transport system, ATP-binding protein
B: Putative ABC transport system integral membrane protein
C: Putative ABC transport system, ATP-binding protein
D: Putative ABC transport system integral membrane protein
E: Putative ABC transport system, ATP-binding protein
F: Putative ABC transport system integral membrane protein
G: Putative ABC transport system, ATP-binding protein
H: Putative ABC transport system integral membrane protein
I: Putative ABC transport system, ATP-binding protein
J: Putative ABC transport system integral membrane protein
K: Putative ABC transport system, ATP-binding protein
L: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)366,35418
Polymers364,30512
Non-polymers2,0496
Water00
1
A: Putative ABC transport system, ATP-binding protein
B: Putative ABC transport system integral membrane protein
C: Putative ABC transport system, ATP-binding protein
D: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)122,7628
Polymers121,4354
Non-polymers1,3274
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12420 Å2
ΔGint-94 kcal/mol
Surface area45790 Å2
MethodPISA
2
E: Putative ABC transport system, ATP-binding protein
F: Putative ABC transport system integral membrane protein
G: Putative ABC transport system, ATP-binding protein
H: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)122,1576
Polymers121,4354
Non-polymers7222
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10620 Å2
ΔGint-83 kcal/mol
Surface area47190 Å2
MethodPISA
3
I: Putative ABC transport system, ATP-binding protein
J: Putative ABC transport system integral membrane protein
K: Putative ABC transport system, ATP-binding protein
L: Putative ABC transport system integral membrane protein


Theoretical massNumber of molelcules
Total (without water)121,4354
Polymers121,4354
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8320 Å2
ΔGint-53 kcal/mol
Surface area47900 Å2
MethodPISA
Unit cell
Length a, b, c (Å)82.001, 133.485, 159.341
Angle α, β, γ (deg.)111.650, 99.690, 94.640
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and resid 5 through 218)
21(chain C and resid 5 through 218)
31(chain E and resid 5 through 218)
41(chain G and resid 5 through 218)
51(chain I and resid 5 through 218)
61(chain K and resid 5 through 218)
12(chain B and (resid 1 through 219 or resid 221 through 341))
22(chain D and (resid 1 through 219 or resid 221 through 341))
32(chain F and (resid 1 through 219 or resid 221 through 341))
42(chain H and (resid 1 through 219 or resid 221 through 341))
52(chain J and (resid 1 through 219 or resid 221 through 341))
62(chain L and (resid 1 through 219 or resid 221 through 341))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111PROPROLEULEU(chain A and resid 5 through 218)AA5 - 2185 - 218
211PROPROLEULEU(chain C and resid 5 through 218)CC5 - 2185 - 218
311PROPROLEULEU(chain E and resid 5 through 218)EE5 - 2185 - 218
411PROPROLEULEU(chain G and resid 5 through 218)GG5 - 2185 - 218
511PROPROLEULEU(chain I and resid 5 through 218)II5 - 2185 - 218
611PROPROLEULEU(chain K and resid 5 through 218)KK5 - 2185 - 218
112METMETGLUGLU(chain B and (resid 1 through 219 or resid 221 through 341))BB1 - 2191 - 219
122SERSERGLYGLY(chain B and (resid 1 through 219 or resid 221 through 341))BB221 - 341221 - 341
212METMETGLUGLU(chain D and (resid 1 through 219 or resid 221 through 341))DD1 - 2191 - 219
222SERSERGLYGLY(chain D and (resid 1 through 219 or resid 221 through 341))DD221 - 341221 - 341
312METMETGLUGLU(chain F and (resid 1 through 219 or resid 221 through 341))FF1 - 2191 - 219
322SERSERGLYGLY(chain F and (resid 1 through 219 or resid 221 through 341))FF221 - 341221 - 341
412METMETGLUGLU(chain H and (resid 1 through 219 or resid 221 through 341))HH1 - 2191 - 219
422SERSERGLYGLY(chain H and (resid 1 through 219 or resid 221 through 341))HH221 - 341221 - 341
512METMETGLUGLU(chain J and (resid 1 through 219 or resid 221 through 341))JJ1 - 2191 - 219
522SERSERGLYGLY(chain J and (resid 1 through 219 or resid 221 through 341))JJ221 - 341221 - 341
612METMETGLUGLU(chain L and (resid 1 through 219 or resid 221 through 341))LL1 - 2191 - 219
622SERSERGLYGLY(chain L and (resid 1 through 219 or resid 221 through 341))LL221 - 341221 - 341

NCS ensembles :
ID
1
2

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Components

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Putative ABC transport ... , 2 types, 12 molecules ACEGIKBDFHJL

#1: Protein
Putative ABC transport system, ATP-binding protein


Mass: 24851.355 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Corynebacterium diphtheriae NCTC 13129 (bacteria)
Strain: ATCC 700971 / NCTC 13129 / Biotype gravis / Gene: DIP2323 / Plasmid: pRSET-C / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Star / References: UniProt: Q6NEF2
#2: Protein
Putative ABC transport system integral membrane protein


Mass: 35866.180 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Corynebacterium diphtheriae NCTC 13129 (bacteria)
Strain: ATCC 700971 / NCTC 13129 / Biotype gravis / Gene: DIP2324 / Plasmid: pRSET-C / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Star / References: UniProt: Q6NEF1

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Non-polymers , 4 types, 6 molecules

#3: Chemical ChemComp-AGA / (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE / PHOSPHATIDYL GLYCEROL


Mass: 455.457 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C19H36O10P
#4: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O4
#5: Chemical ChemComp-MNR / PROTOPORPHYRIN IX CONTAINING MN


Mass: 615.580 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C34H32MnN4O4 / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H10O3

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.33 Å3/Da / Density % sol: 71.61 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 22% PEG 3000, 0.2 M Mg acetate, 0.2 M KCl, 0.1 M HEPES pH 7.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL32XU / Wavelength: 1 Å
DetectorType: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Jun 5, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.99→49.39 Å / Num. obs: 239883 / % possible obs: 97.2 % / Redundancy: 3.663 % / Biso Wilson estimate: 90.429 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.168 / Rrim(I) all: 0.197 / Χ2: 1.129 / Net I/σ(I): 4.77 / Num. measured all: 878625
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
2.99-3.173.7991.9060.4414657139870385820.3032.21996.8
3.17-3.393.7681.1180.9113774237378365550.551.30597.8
3.39-3.663.6720.6591.712459834772339360.7890.77397.6
3.66-4.013.420.3782.9810617632022310440.8860.45196.9
4.01-4.483.5510.2085.3610004729088281740.960.24696.9
4.48-5.173.7680.1617.569382325602249020.9770.18897.3
5.17-6.313.7170.1547.687817521714210300.9770.1896.8
6.31-8.853.4070.08712.355537816776162520.9910.10496.9
8.85-49.393.8390.04525.7336115956894080.9980.05398.3

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Processing

Software
NameVersionClassification
XDSdata scaling
PHENIX1.18.2_3874refinement
PDB_EXTRACT3.25data extraction
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6LLJ

6llj
PDB Unreleased entry


Resolution: 3→49.39 Å / SU ML: 0.68 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 38.75 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2988 7407 3.14 %
Rwork0.2664 228749 -
obs0.2674 236156 96.45 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 317.34 Å2 / Biso mean: 134.3242 Å2 / Biso min: 73.08 Å2
Refinement stepCycle: final / Resolution: 3→49.39 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms24966 0 133 0 25099
Biso mean--149.52 --
Num. residues----3369
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6077X-RAY DIFFRACTION11.52TORSIONAL
12C6077X-RAY DIFFRACTION11.52TORSIONAL
13E6077X-RAY DIFFRACTION11.52TORSIONAL
14G6077X-RAY DIFFRACTION11.52TORSIONAL
15I6077X-RAY DIFFRACTION11.52TORSIONAL
16K6077X-RAY DIFFRACTION11.52TORSIONAL
21B9186X-RAY DIFFRACTION11.52TORSIONAL
22D9186X-RAY DIFFRACTION11.52TORSIONAL
23F9186X-RAY DIFFRACTION11.52TORSIONAL
24H9186X-RAY DIFFRACTION11.52TORSIONAL
25J9186X-RAY DIFFRACTION11.52TORSIONAL
26L9186X-RAY DIFFRACTION11.52TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3-3.030.45442240.43656588681282
3.03-3.070.44432060.44467020722690
3.07-3.110.40852310.42697400763193
3.11-3.150.50292410.47157681792297
3.15-3.190.49032900.46067681797198
3.19-3.230.46022600.43077582784298
3.23-3.280.41782200.40857861808198
3.28-3.330.37062500.38747697794798
3.33-3.380.40793010.37487642794398
3.38-3.430.3632490.36567754800398
3.43-3.490.31652000.35287731793198
3.49-3.560.33092670.34847766803398
3.56-3.630.33792490.34537695794497
3.63-3.70.33462510.34117696794798
3.7-3.780.34252780.30437647792597
3.78-3.870.36032240.29717752797697
3.87-3.960.31692310.28597602783397
3.96-4.070.30032680.27427682795097
4.07-4.190.31742260.25127690791697
4.19-4.330.29472890.24557648793797
4.33-4.480.23762230.22877660788397
4.48-4.660.2722600.21847666792697
4.66-4.870.25422540.22147750800498
4.87-5.130.27632420.22747741798397
5.13-5.450.31072470.24017614786197
5.45-5.870.27632450.24377699794497
5.87-6.460.24922500.24077662791297
6.46-7.390.23572510.22467701795297
7.39-9.30.20952370.18127669790697
9.3-49.390.29232430.23847772801598
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.81940.1134-0.14734.68713.15796.26-0.19770.49020.34390.29250.3296-0.2561-0.02250.8839-0.08480.71580.0041-0.03490.97520.15370.767993.418-17.60474.484
24.4747-0.26631.61933.49380.78191.0725-0.1169-0.3866-0.52120.3956-1.4373-0.96980.83042.95950.27910.97110.37190.04991.97770.5160.753369.144-13.7438.974
35.74132.4112-0.14498.6611-2.09488.08060.00490.29420.0371-0.97190.14560.84050.2899-0.8702-0.26851.47990.2736-0.15241.30640.22060.801552.5835.4692.483
42.6768-1.51370.95212.7258-0.61594.59650.13280.88210.4861-0.7995-0.2701-0.017-0.621-0.36750.43490.75140.04010.19050.88680.26340.657568.759-3.18438.541
54.6837-1.98270.0735.6106-0.12764.6887-0.2154-0.00090.03450.03850.37450.09080.1982-0.1139-0.20320.71390.04740.03560.61460.14010.662965.272-25.81886.553
61.9524-1.81690.74734.3978-2.43266.5386-0.05290.59930.7388-0.3036-0.9442-0.2104-1.263-1.22480.7172.1160.703-0.05132.11280.33460.967651.638-7.05449.865
73.3107-1.2669-1.22763.39120.17222.44540.1759-0.2062-0.2751-0.39840.33141.0214-0.0552-1.7576-0.5211.4276-0.0235-0.22872.480.47621.299530.046-1.54111.266
81.24941.87961.1521.74541.13334.8107-0.2456-0.2517-0.3173-0.78040.92770.5646-0.5939-2.1057-0.62221.30850.0691-0.00121.51630.42591.017849.282-15.66644.458
99.7123-2.58133.48255.54172.77635.96290.32740.5854-0.46790.12180.1622-0.86510.95971.6297-0.4931.26290.06960.2251.0772-0.04220.997797.443-4.27126.032
103.87791.19310.2982.5048-0.63060.7347-0.19371.1967-0.2716-0.6006-0.7437-0.87280.5999-0.06471.04791.51370.51470.16181.12-0.06710.914769.0256.455156.807
116.3165-0.0699-4.30414.77311.11029.6564-0.5865-0.6034-0.20940.11880.15560.82110.0402-0.93830.28361.34530.37410.14341.5204-0.14740.750555.74621.401196.633
120.9813-0.62911.37783.2087-0.4737.00240.038-0.53680.2880.4010.3102-0.239-0.6562-0.422-0.38890.8494-0.0732-0.04650.7681-0.25190.753275.8515.668164.835
136.043-1.4405-0.79793.84040.43958.50240.0777-0.65180.42260.02460.0316-0.07130.35630.8027-0.00010.63510.13220.01060.7002-0.17370.672677.662-30.814124.783
142.15782.22891.93662.6432.11774.2916-0.6309-0.226-0.31742.76081.17061.74640.66270.2644-0.38582.63080.78430.64421.80110.24091.160368.074-13.688164.348
150.8285-0.78140.70084.20310.58562.8794-0.36920.2169-0.82610.9438-0.42090.60351.8888-1.21750.71272.3012-0.01390.6892.0745-0.28721.331743.372-0.581198.748
163.3122-1.2992-3.8861.07251.39233.1274-0.3702-0.28950.23270.9830.28650.17221.0776-0.47150.08511.91760.13990.27741.7598-0.25631.088658.295-10.774162.301
172.6545-0.7592-0.8693.3898-0.50623.84970.15770.1965-0.3458-0.3341-0.08680.17750.0279-0.3022-0.06510.87640.2428-0.0830.7735-0.08070.881418.445-47.7294.681
183.134-3.14660.19388.93241.77873.75210.1140.3453-1.5299-1.1846-0.88720.28291.11730.2920.31251.64860.51340.45111.17610.07662.403445.132-82.02688.994
194.8912-0.5773-2.20622.47853.29874.80910.48860.04471.076-0.8115-0.1832-0.3488-0.374-0.2137-0.20341.55260.48170.10410.96970.15141.820257.062-123.77193.151
204.1651-1.5294-1.05993.0482-1.37553.2661-0.6539-0.3872-0.92830.5210.306-0.30151.38270.93650.20051.47230.5301-0.10480.8421-0.11721.110738.74-79.71694.08
214.5914-0.3081-1.24175.9719-1.96296.50260.3415-0.11030.2688-0.2622-0.3365-0.2947-0.32490.0203-0.04960.5870.0782-0.01150.4261-0.00370.594441.548-38.296114.246
220.69790.3157-1.50051.535-2.31544.41660.28870.2984-0.31090.31850.7006-0.18450.50730.2913-0.88891.39470.1909-0.40040.928-0.13851.645750.849-80.974117.419
236.603-2.74930.27327.1982.97466.52130.15850.47220.1848-0.4632-0.41590.90160.0161-0.10540.3391.54740.29280.02320.76540.03181.470376.447-115.744110.154
243.2104-0.81430.13773.05082.79612.2213-0.3096-0.2665-0.85580.93240.508-0.05690.6017-0.0795-0.08741.55430.226-0.09140.83930.07591.182352.897-74.037112.706
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 3:218 )A3 - 218
2X-RAY DIFFRACTION2( CHAIN B AND RESID 1:42 )B1 - 42
3X-RAY DIFFRACTION3( CHAIN B AND RESID 43:189 )B43 - 189
4X-RAY DIFFRACTION4( CHAIN B AND RESID 190:341 )B190 - 341
5X-RAY DIFFRACTION5( CHAIN C AND RESID 3:219 )C3 - 219
6X-RAY DIFFRACTION6( CHAIN D AND RESID 1:42 )D1 - 42
7X-RAY DIFFRACTION7( CHAIN D AND RESID 43:189 )D43 - 189
8X-RAY DIFFRACTION8( CHAIN D AND RESID 190:342 )D190 - 342
9X-RAY DIFFRACTION9( CHAIN E AND RESID 5:218 )E5 - 218
10X-RAY DIFFRACTION10( CHAIN F AND RESID 1:42 )F1 - 42
11X-RAY DIFFRACTION11( CHAIN F AND RESID 43:189 )F43 - 189
12X-RAY DIFFRACTION12( CHAIN F AND RESID 190:342 )F190 - 342
13X-RAY DIFFRACTION13( CHAIN G AND RESID 3:225 )G3 - 225
14X-RAY DIFFRACTION14( CHAIN H AND RESID 1:42 )H1 - 42
15X-RAY DIFFRACTION15( CHAIN H AND RESID 43:189 )H43 - 189
16X-RAY DIFFRACTION16( CHAIN H AND RESID 190:341 )H190 - 341
17X-RAY DIFFRACTION17( CHAIN I AND RESID 3:223 )I3 - 223
18X-RAY DIFFRACTION18( CHAIN J AND RESID 1:42 )J1 - 42
19X-RAY DIFFRACTION19( CHAIN J AND RESID 43:219 )J43 - 219
20X-RAY DIFFRACTION20( CHAIN J AND RESID 220:343 )J220 - 343
21X-RAY DIFFRACTION21( CHAIN K AND RESID 4:221 )K4 - 221
22X-RAY DIFFRACTION22( CHAIN L AND RESID 1:42 )L1 - 42
23X-RAY DIFFRACTION23( CHAIN L AND RESID 43:219 )L43 - 219
24X-RAY DIFFRACTION24( CHAIN L AND RESID 220:343 )L220 - 343

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