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- PDB-7w7a: Heme exporter in complex with Mn-containing protoporphyrin IX, Mn... -

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Basic information

Entry
Database: PDB / ID: 7w7a
TitleHeme exporter in complex with Mn-containing protoporphyrin IX, Mn-anomalous data
Components(Putative ABC transport ...) x 2
KeywordsMEMBRANE PROTEIN / Heme efflux / Heme detoxification / Heme extraction / ABC transporter / 4-Helix TMD
Function / homology
Function and homology information


transmembrane transporter activity / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane
Similarity search - Function
MacB-like periplasmic core domain / MacB-like periplasmic core domain / MacB, ATP-binding domain / ABC transporter, lipoprotein release, LolD / ABC3 transporter permease protein domain / FtsX-like permease family / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain ...MacB-like periplasmic core domain / MacB-like periplasmic core domain / MacB, ATP-binding domain / ABC transporter, lipoprotein release, LolD / ABC3 transporter permease protein domain / FtsX-like permease family / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Chem-AGA / PROTOPORPHYRIN IX CONTAINING MN / DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / ABC transport system integral membrane protein / ABC transport system, ATP-binding protein
Similarity search - Component
Biological speciesCorynebacterium diphtheriae NCTC 13129 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.204 Å
AuthorsHisano, T. / Nakamura, H. / Rahman, M.M. / Tosha, T. / Shirouzu, M. / Shiro, Y.
Funding support Japan, 4items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)26220807 Japan
Japan Society for the Promotion of Science (JSPS)16K07309 Japan
Japan Society for the Promotion of Science (JSPS)JP19H00926 Japan
Japan Society for the Promotion of Science (JSPS)JP19H05761 Japan
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2022
Title: Structural basis for heme detoxification by an ATP-binding cassette-type efflux pump in gram-positive pathogenic bacteria.
Authors: Nakamura, H. / Hisano, T. / Rahman, M.M. / Tosha, T. / Shirouzu, M. / Shiro, Y.
History
DepositionDec 4, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 22, 2022Provider: repository / Type: Initial release
Revision 1.1Dec 21, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Putative ABC transport system, ATP-binding protein
B: Putative ABC transport system integral membrane protein
C: Putative ABC transport system, ATP-binding protein
D: Putative ABC transport system integral membrane protein
E: Putative ABC transport system, ATP-binding protein
F: Putative ABC transport system integral membrane protein
G: Putative ABC transport system, ATP-binding protein
H: Putative ABC transport system integral membrane protein
I: Putative ABC transport system, ATP-binding protein
J: Putative ABC transport system integral membrane protein
K: Putative ABC transport system, ATP-binding protein
L: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)366,31018
Polymers364,30512
Non-polymers2,0056
Water00
1
A: Putative ABC transport system, ATP-binding protein
B: Putative ABC transport system integral membrane protein
C: Putative ABC transport system, ATP-binding protein
D: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)122,7628
Polymers121,4354
Non-polymers1,3274
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12460 Å2
ΔGint-91 kcal/mol
Surface area46010 Å2
MethodPISA
2
E: Putative ABC transport system, ATP-binding protein
F: Putative ABC transport system integral membrane protein
G: Putative ABC transport system, ATP-binding protein
H: Putative ABC transport system integral membrane protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)122,1136
Polymers121,4354
Non-polymers6782
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10960 Å2
ΔGint-84 kcal/mol
Surface area47190 Å2
MethodPISA
3
I: Putative ABC transport system, ATP-binding protein
J: Putative ABC transport system integral membrane protein
K: Putative ABC transport system, ATP-binding protein
L: Putative ABC transport system integral membrane protein


Theoretical massNumber of molelcules
Total (without water)121,4354
Polymers121,4354
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8330 Å2
ΔGint-51 kcal/mol
Surface area48710 Å2
MethodPISA
Unit cell
Length a, b, c (Å)82.488, 133.672, 159.838
Angle α, β, γ (deg.)111.810, 99.830, 94.430
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and resid 5 through 217)
21(chain C and resid 5 through 217)
31chain E
41(chain G and resid 5 through 217)
51(chain I and resid 5 through 217)
61(chain K and resid 5 through 217)
12(chain B and resid 1 through 341)
22(chain D and resid 1 through 341)
32(chain F and resid 1 through 341)
42(chain H and resid 1 through 341)
52(chain J and resid 1 through 341)
62(chain L and resid 1 through 341)

NCS domain segments:

Component-ID: 1

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11PROPROALAALA(chain A and resid 5 through 217)AA5 - 2175 - 217
21PROPROALAALA(chain C and resid 5 through 217)CC5 - 2175 - 217
31PROPROALAALAchain EEE5 - 2175 - 217
41PROPROALAALA(chain G and resid 5 through 217)GG5 - 2175 - 217
51PROPROALAALA(chain I and resid 5 through 217)II5 - 2175 - 217
61PROPROALAALA(chain K and resid 5 through 217)KK5 - 2175 - 217
12METMETGLYGLY(chain B and resid 1 through 341)BB1 - 3411 - 341
22METMETGLYGLY(chain D and resid 1 through 341)DD1 - 3411 - 341
32METMETGLYGLY(chain F and resid 1 through 341)FF1 - 3411 - 341
42METMETGLYGLY(chain H and resid 1 through 341)HH1 - 3411 - 341
52METMETGLYGLY(chain J and resid 1 through 341)JJ1 - 3411 - 341
62METMETGLYGLY(chain L and resid 1 through 341)LL1 - 3411 - 341

NCS ensembles :
ID
1
2

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Components

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Putative ABC transport ... , 2 types, 12 molecules ACEGIKBDFHJL

#1: Protein
Putative ABC transport system, ATP-binding protein


Mass: 24851.355 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Corynebacterium diphtheriae NCTC 13129 (bacteria)
Strain: ATCC 700971 / NCTC 13129 / Biotype gravis / Gene: DIP2323 / Plasmid: pRSET-C / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Star / References: UniProt: Q6NEF2
#2: Protein
Putative ABC transport system integral membrane protein


Mass: 35866.180 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Corynebacterium diphtheriae NCTC 13129 (bacteria)
Strain: ATCC 700971 / NCTC 13129 / Biotype gravis / Gene: DIP2324 / Plasmid: pRSET-C / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Star / References: UniProt: Q6NEF1

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Non-polymers , 5 types, 6 molecules

#3: Chemical ChemComp-AGA / (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE / PHOSPHATIDYL GLYCEROL


Mass: 455.457 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C19H36O10P
#4: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O4
#5: Chemical ChemComp-MNR / PROTOPORPHYRIN IX CONTAINING MN


Mass: 615.580 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C34H32MnN4O4 / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#7: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.37 Å3/Da / Density % sol: 71.87 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 22% PEG 3000, 0.2 M Mg acetate, 0.2 M KCl, 0.1 M HEPES pH 7.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B2 / Wavelength: 1.89 Å
DetectorType: RAYONIX MX225-HS / Detector: CCD / Date: May 11, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.89 Å / Relative weight: 1
ReflectionResolution: 3.2→48.82 Å / Num. obs: 196361 / % possible obs: 96.6 % / Redundancy: 3.963 % / Biso Wilson estimate: 98.88 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.128 / Rrim(I) all: 0.149 / Χ2: 1.169 / Net I/σ(I): 7.75 / Num. measured all: 778250
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.2-3.43.9731.640.9812064232704303680.541.89692.9
3.4-3.633.9830.8551.8811802930872296360.8750.98896
3.63-3.923.9810.4543.4911032328694277100.950.52596.6
3.92-4.293.9820.2525.8310179626328255650.970.29197.1
4.29-4.793.9660.1439.439264123970233590.9870.16697.5
4.79-5.523.9620.12510.298187321094206650.990.14498
5.52-6.753.9590.11211.736953717836175630.990.12998.5
6.75-9.453.8950.05321.525332913852136900.9970.06198.8
9.45-48.823.8540.03232.8830080794078050.9990.03798.3

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Processing

Software
NameVersionClassification
XSCALEdata scaling
PHENIX1.13_2998refinement
PDB_EXTRACT3.25data extraction
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6LLI

6lli
PDB Unreleased entry


Resolution: 3.204→48.82 Å / SU ML: 0.51 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 36.89 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.3029 6131 3.13 %
Rwork0.264 189985 -
obs0.2652 196116 96.65 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 485.43 Å2 / Biso mean: 131.611 Å2 / Biso min: 67.43 Å2
Refinement stepCycle: final / Resolution: 3.204→48.82 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms24970 0 132 0 25102
Biso mean--275.56 --
Num. residues----3370
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6044X-RAY DIFFRACTION11.627TORSIONAL
12C6044X-RAY DIFFRACTION11.627TORSIONAL
13E6044X-RAY DIFFRACTION11.627TORSIONAL
14G6044X-RAY DIFFRACTION11.627TORSIONAL
15I6044X-RAY DIFFRACTION11.627TORSIONAL
16K6044X-RAY DIFFRACTION11.627TORSIONAL
21B9220X-RAY DIFFRACTION11.627TORSIONAL
22D9220X-RAY DIFFRACTION11.627TORSIONAL
23F9220X-RAY DIFFRACTION11.627TORSIONAL
24H9220X-RAY DIFFRACTION11.627TORSIONAL
25J9220X-RAY DIFFRACTION11.627TORSIONAL
26L9220X-RAY DIFFRACTION11.627TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.204-3.23990.48311780.4555529381
3.2399-3.27810.42941860.4543625395
3.2781-3.3180.46652000.4406626795
3.318-3.360.42772330.423627396
3.36-3.40420.39672340.4054624196
3.4042-3.45080.39832010.3989629596
3.4508-3.50010.3761480.3802625496
3.5001-3.55240.3542260.3604635196
3.5524-3.60780.40332060.347632996
3.6078-3.6670.37231900.3185621797
3.667-3.73020.3362120.3208639597
3.7302-3.7980.37092180.3016629697
3.798-3.8710.35461860.3017633197
3.871-3.950.32871970.296638397
3.95-4.03580.30852060.2896629497
4.0358-4.12970.2932080.2814640697
4.1297-4.23290.32461980.2583630697
4.2329-4.34730.30392560.242647698
4.3473-4.47510.24431780.2392634598
4.4751-4.61950.28472000.2284644398
4.6195-4.78440.30222260.2295642698
4.7844-4.97580.2692040.2322636598
4.9758-5.2020.29232000.2339647298
5.202-5.47590.3152200.2551640498
5.4759-5.81850.29881960.2476650298
5.8185-6.26690.2711950.2454641099
6.2669-6.8960.22512110.2351652799
6.896-7.89030.23922020.2212643999
7.8903-9.92710.18282130.1614651999
9.9271-48.820.32842030.2563647399
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.8147-0.4885-0.57832.1731.79243.5559-0.20060.43990.27520.3030.1889-0.39080.1821.0112-0.14010.4973-0.4087-0.05641.07-0.03061.059293.96-17.8474.291
23.0327-0.05920.07293.64570.73863.5258-0.2467-0.02590.06290.274-0.251-0.81310.6431.33780.85910.60220.01590.05340.80010.06080.498169.841-13.77438.534
35.5612.2882-0.79166.6165-2.1523.6316-0.08980.23320.0474-0.35450.13720.5518-0.2666-0.7585-0.13921.29830.3578-0.15921.460.08270.801953.3985.5692.055
41.4674-1.19220.96663.0544-0.15682.24030.1090.52410.3236-0.7143-0.27790.0797-0.9325-0.29310.24350.85010.0140.24731.20610.19830.842869.571-3.2538.32
54.4393-2.31730.26366.2772-0.37144.1259-0.11920.117-0.11370.05860.34970.25280.1567-0.1274-0.24810.5221-0.05170.0320.53250.09680.610265.712-26.25186.132
61.21890.6846-0.70171.9682-0.28570.40220.08010.06810.5055-0.2444-1.10560.0657-1.4046-0.13080.70032.16640.8456-0.08912.68970.36090.963152.285-7.17549.459
75.3476-0.9159-0.67921.7075-0.7193.63330.625-0.5359-0.79-0.60320.52941.63390.474-2.8739-0.90991.5193-0.1245-0.48262.60560.50131.259830.731-1.56210.73
80.1820.57220.23771.46480.2352.9590.06990.0207-0.1451-1.05180.4660.5014-0.1123-1.4219-0.38881.0941-0.2832-0.1651.24670.25091.048750.006-15.90244.124
96.693-2.40442.72665.44591.75755.197-0.14470.3901-0.12720.16540.3964-0.75280.43021.3917-0.28021.0434-0.04970.26750.9163-0.09651.013197.861-4.546125.813
102.00371.0330.11011.8864-0.59933.14890.0481.0758-0.4193-0.6531-0.973-1.06940.40450.2070.90941.35820.55220.12871.2922-0.07120.825869.4186.043156.695
115.7023-0.667-3.54414.17110.91716.9327-0.5213-0.9992-0.03910.41840.2110.47810.0574-0.57410.38531.14750.25880.11371.4202-0.18810.776156.20320.872196.535
120.418-0.5632-0.05132.19910.30076.4384-0.0572-0.55620.47160.64060.3832-0.271-0.7789-0.6047-0.25181.15610.1198-0.13731.2436-0.38210.965476.3945.16164.543
135.8425-1.0573-1.2794.23830.70888.4356-0.0438-0.68050.12260.104-0.00440.00870.44380.55040.12140.650.0643-0.01240.7415-0.19370.709878.124-31.311124.552
142.6652.13350.91171.8881.54974.4895-0.6904-0.2718-0.00772.45611.11192.01720.0717-0.54430.01052.00410.41350.64141.53480.1811.150368.466-14.219164.193
150.9762-1.32110.48723.97041.45832.3147-0.30510.0045-0.47020.7256-0.45080.53571.4967-0.63410.72242.28630.10460.83392.0788-0.32141.559243.763-1.041198.633
163.2341-2.0854-2.47973.76361.23451.8933-0.3393-0.05890.10410.52320.46940.05230.4522-0.6238-0.27291.2680.0590.18381.5694-0.16410.987658.777-11.244161.948
173.1163-1.1492-0.97234.14940.04154.36660.21040.2676-0.376-0.4139-0.10470.26520.2653-0.3611-0.15360.69690.0592-0.10590.6912-0.12210.788418.484-48.20694.483
181.23040.1061-0.42591.07520.83550.8670.73850.4951-0.7908-0.9293-0.56031.02320.88430.80250.03282.06741.1060.23971.73090.15052.235645.117-82.63788.668
194.5542-0.8914-1.41435.1841.97545.41560.418-0.05761.3006-0.5701-0.0046-0.3937-0.5445-0.3137-0.40591.20680.3265-0.09540.8327-0.05431.616157.03-124.41992.688
203.1511-0.9559-1.17721.8691-0.17720.6087-0.7653-0.6495-1.10140.88310.6061-0.10541.50370.83390.11831.89270.7576-0.09371.1139-0.17161.319138.563-80.08493.768
215.2686-1.1038-1.37975.2427-1.23565.75660.64710.30410.393-0.4279-0.4986-0.3225-0.5267-0.0054-0.09250.5864-0.09240.00370.5463-0.01370.749741.826-38.734113.913
221.2887-0.2076-1.05561.5304-0.52931.15991.0170.7777-0.6205-0.91260.0216-0.03021.43060.7214-0.49172.050.4745-0.57531.0226-0.261.788950.965-81.531117.103
236.4914-2.46450.04938.21232.34696.38960.40820.74580.1832-0.8013-0.5950.8077-0.1871-0.04420.2811.50560.35150.03440.9982-0.05151.468376.428-116.351109.888
243.27-2.1652-0.11434.81733.01762.75690.09140.1197-0.71180.46770.28890.03850.4227-0.0919-0.23421.13440.0525-0.17930.5873-0.05131.123753.061-74.545112.377
250.271-0.0722-0.3410.01950.08770.45980.06710.3348-0.0792-0.2156-0.0489-0.01720.1243-0.09510.05892.9173-0.24091.21574.1037-0.82772.138249.653-3.79421.121
261.0608-0.0166-0.14550.02320.0360.0696-0.08180.30230.07920.1232-0.0218-0.396-0.1090.30820.09623.9637-1.5243-2.29945.01161.57659.337756.5195.028182.34
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 3:218 )A3 - 218
2X-RAY DIFFRACTION2( CHAIN B AND RESID 1:42 )B1 - 42
3X-RAY DIFFRACTION3( CHAIN B AND RESID 43:189 )B43 - 189
4X-RAY DIFFRACTION4( CHAIN B AND RESID 190:341 )B190 - 341
5X-RAY DIFFRACTION5( CHAIN C AND RESID 3:219 )C3 - 219
6X-RAY DIFFRACTION6( CHAIN D AND RESID 1:42 )D1 - 42
7X-RAY DIFFRACTION7( CHAIN D AND RESID 43:189 )D43 - 189
8X-RAY DIFFRACTION8( CHAIN D AND RESID 190:342 )D190 - 342
9X-RAY DIFFRACTION9( CHAIN E AND RESID 5:217 )E5 - 217
10X-RAY DIFFRACTION10( CHAIN F AND RESID 1:42 )F1 - 42
11X-RAY DIFFRACTION11( CHAIN F AND RESID 43:189 )F43 - 189
12X-RAY DIFFRACTION12( CHAIN F AND RESID 190:342 )F190 - 342
13X-RAY DIFFRACTION13( CHAIN G AND RESID 3:225 )G3 - 225
14X-RAY DIFFRACTION14( CHAIN H AND RESID 1:42 )H1 - 42
15X-RAY DIFFRACTION15( CHAIN H AND RESID 43:189 )H43 - 189
16X-RAY DIFFRACTION16( CHAIN H AND RESID 190:342 )H190 - 342
17X-RAY DIFFRACTION17( CHAIN I AND RESID 3:223 )I3 - 223
18X-RAY DIFFRACTION18( CHAIN J AND RESID 1:42 )J1 - 42
19X-RAY DIFFRACTION19( CHAIN J AND RESID 43:219 )J43 - 219
20X-RAY DIFFRACTION20( CHAIN J AND RESID 220:343 )J220 - 343
21X-RAY DIFFRACTION21( CHAIN K AND RESID 4:221 )K4 - 221
22X-RAY DIFFRACTION22( CHAIN L AND RESID 1:42 )L1 - 42
23X-RAY DIFFRACTION23( CHAIN L AND RESID 43:219 )L43 - 219
24X-RAY DIFFRACTION24( CHAIN L AND RESID 220:343 )L220 - 343
25X-RAY DIFFRACTION25( CHAIN D AND RESID 401:401 )D401
26X-RAY DIFFRACTION26( CHAIN H AND RESID 401:401 )H401

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