+Open data
-Basic information
Entry | Database: PDB / ID: 7vu5 | ||||||
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Title | Structure of the transmembrane domain of the CD28 dimer | ||||||
Components | T-cell-specific surface glycoprotein CD28 | ||||||
Keywords | IMMUNE SYSTEM / CD28 / transmembrane domain | ||||||
Function / homology | Function and homology information Nef mediated downregulation of CD28 cell surface expression / positive regulation of inflammatory response to antigenic stimulus / regulatory T cell differentiation / protein complex involved in cell adhesion / regulation of regulatory T cell differentiation / CD4-positive, alpha-beta T cell proliferation / positive regulation of isotype switching to IgG isotypes / negative thymic T cell selection / positive regulation of CD4-positive, alpha-beta T cell proliferation / CD28 co-stimulation ...Nef mediated downregulation of CD28 cell surface expression / positive regulation of inflammatory response to antigenic stimulus / regulatory T cell differentiation / protein complex involved in cell adhesion / regulation of regulatory T cell differentiation / CD4-positive, alpha-beta T cell proliferation / positive regulation of isotype switching to IgG isotypes / negative thymic T cell selection / positive regulation of CD4-positive, alpha-beta T cell proliferation / CD28 co-stimulation / CD28 dependent Vav1 pathway / CD28 dependent PI3K/Akt signaling / positive regulation of interleukin-10 production / humoral immune response / immunological synapse / positive regulation of interleukin-4 production / positive regulation of viral genome replication / coreceptor activity / positive regulation of T cell proliferation / T cell costimulation / positive regulation of interleukin-2 production / T cell activation / positive regulation of mitotic nuclear division / positive regulation of translation / phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cytokine production / apoptotic signaling pathway / Constitutive Signaling by Aberrant PI3K in Cancer / PIP3 activates AKT signaling / T cell receptor signaling pathway / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protease binding / transcription by RNA polymerase II / cell surface receptor signaling pathway / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / external side of plasma membrane / negative regulation of gene expression / positive regulation of gene expression / negative regulation of apoptotic process / protein kinase binding / cell surface / positive regulation of transcription by RNA polymerase II / identical protein binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wu, H. / Cao, R. / Wen, M. / Ouyang, B. | ||||||
Funding support | China, 1items
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Citation | Journal: Structure / Year: 2022 Title: Structural characterization of a dimerization interface in the CD28 transmembrane domain. Authors: Wu, H. / Cao, R. / Wen, M. / Xue, H. / OuYang, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7vu5.cif.gz | 405.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7vu5.ent.gz | 340.8 KB | Display | PDB format |
PDBx/mmJSON format | 7vu5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vu/7vu5 ftp://data.pdbj.org/pub/pdb/validation_reports/vu/7vu5 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4665.656 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD28 / Production host: Escherichia coli (E. coli) / References: UniProt: P10747 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 25 mM / Label: conditions_1 / pH: 6.0 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: 15 / Conformers calculated total number: 100 / Conformers submitted total number: 15 |