PDB-7vn5: Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with ...

ID or keywords:

Entry

Database: PDB / ID: 7vn5

Title

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning TTCACGTGAA

Components

DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')
Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

Keywords

TRANSCRIPTION / Transcription factor-DNA complex

Function / homology

Function and homology information

plant ovule development / seed development / brassinosteroid mediated signaling pathway / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / periplasmic space / DNA-binding transcription factor activity ...
Similarity search - Function

Biological species

Serratia sp. (bacteria)

Arabidopsis thaliana (thale cress)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.95 Å

Authors

Nosaki, S. / Tanokura, M. / Miyakawa, T.

Funding support

Japan, 2items

Citation

Journal: Nat.Plants / Year: 2022
Title: Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout.
Authors: Nosaki, S. / Mitsuda, N. / Sakamoto, S. / Kusubayashi, K. / Yamagami, A. / Xu, Y. / Bui, T.B.C. / Terada, T. / Miura, K. / Nakano, T. / Tanokura, M. / Miyakawa, T.

History

Deposition	Oct 10, 2021	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Dec 7, 2022	Provider: repository / Type: Initial release
Revision 1.1	Dec 28, 2022	Group: Database references / Category: citation / citation_author Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2	Jan 11, 2023	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3	Nov 29, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	7vn5.cif.gz	855.9 KB	Display	PDBx/mmCIF format
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Validation report

Summary document	7vn5_validation.pdf.gz	2 MB	Display	wwPDB validaton report
Full document	7vn5_full_validation.pdf.gz	2.1 MB	Display
Data in XML	7vn5_validation.xml.gz	67.7 KB	Display
Data in CIF	7vn5_validation.cif.gz	93.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/vn/7vn5 ftp://data.pdbj.org/pub/pdb/validation_reports/vn/7vn5	HTTPS FTP

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Related structure data

Related structure data	7vn2C 7vn3C 7vn4C 7vn6C 7vn7C 7vn8C 5zd4S S: Starting model for refinement C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#67 - Jul 2005 TATA-Binding Protein similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #253 - Jan 2021 Expressome similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #135 - Mar 2011 Integrase similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #131 - Nov 2010 Inteins similarity (1) #125 - May 2010 Parvoviruses similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1) #221 - May 2018 Human Papillomavirus and Vaccines similarity (6) #205 - Jan 2017 Nuclear Pore Complex similarity (6) #90 - Jun 2007 Fatty Acid Synthase similarity (3) #243 - Mar 2020 Voltage-gated Sodium Channels similarity (5) #74 - Feb 2006 alpha-Amylase similarity (1) #208 - Apr 2017 Glucose Transporters similarity (1) #138 - Jun 2011 Glucansucrase similarity (1) #100 - Apr 2008 Adrenergic Receptors similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

C: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

D: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

G: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

H: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

A: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

B: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

E: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

F: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

hetero molecules

214 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

C: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

D: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

G: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

H: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

hetero molecules

defined by author&software
Evidence: isothermal titration calorimetry
107 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

A: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

B: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

E: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

F: DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

hetero molecules

defined by author&software
107 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	102.622, 93.923, 112.750
Angle α, β, γ (deg.)	90.000, 100.646, 90.000
Int Tables number	4
Space group name H-M	P12₁1
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

#1: Protein

Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1 / Protein BIN2 SUBSTRATE 2

Mass: 48419.836 Da / Num. of mol.: 4 / Mutation: D82A,K83A,E172A,N173A,K239A,E359A,K362A,D363A
Source method: isolated from a genetically manipulated source
Details: The fused Maltose binding protein is derived from Escherichia coli O157:H7
Source: (gene. exp.)

Serratia sp. (strain FS14) (bacteria), (gene. exp.)

Arabidopsis thaliana (thale cress)
Strain: FS14 / Gene: malE, JW3994, BZR1, BIS2, At1g75080, F9E10_7 / Production host:

Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A4P1LXE0, UniProt: Q8S307

#2: DNA chain

DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3')

Mass: 4592.022 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

#3: Polysaccharide

alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose

Type: oligosaccharide, Oligosaccharide / Class: Nutrient / Mass: 342.297 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: alpha-maltose

#4: Chemical

ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL

Mass: 62.068 Da / Num. of mol.: 13 / Source method: obtained synthetically / Formula: C₂H₆O₂

#5: Water

ChemComp-HOH / water

Mass: 18.015 Da / Num. of mol.: 411 / Source method: isolated from a natural source / Formula: H₂O

Has ligand of interest

N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.52 Å³/Da / Density % sol: 51.16 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10%

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Data collection

Diffraction	Mean temperature: 95 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Dec 7, 2019
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 1.95→46.96 Å / Num. obs: 152246 / % possible obs: 99.4 % / Redundancy: 3.4 % / B_iso Wilson estimate: 32.12 Å² / CC_1/2: 0.999 / Net I/σ(I): 3.4
Reflection shell	Resolution: 1.95→1.98 Å / Mean I/σ(I) obs: 3.5 / Num. unique obs: 152246 / CC_1/2: 0.999

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Processing

Software

Name	Version	Classification
PHENIX	1.18_3855	refinement
XDS		data reduction
Aimless		data scaling
MOLREP		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5ZD4

5zd4

Resolution: 1.95→35.82 Å / SU ML: 0.2425 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 28.3741
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2439	7634	5.02 %
R_work	0.2091	144384	-
obs	0.2108	152018	99.24 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 47.14 Å²

Refinement step

Cycle: LAST / Resolution: 1.95→35.82 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13592	1220	144	411	15367

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0073	15411
X-RAY DIFFRACTION	f_angle_d	0.8351	21142
X-RAY DIFFRACTION	f_chiral_restr	0.0555	2306
X-RAY DIFFRACTION	f_plane_restr	0.0051	2526
X-RAY DIFFRACTION	f_dihedral_angle_d	23.2833	5761

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.95-1.97	0.35	256	0.3	4844	X-RAY DIFFRACTION	99.94
1.97-2	0.3525	260	0.2795	4788	X-RAY DIFFRACTION	99.88
2-2.02	0.2893	256	0.2768	4836	X-RAY DIFFRACTION	99.82
2.02-2.05	0.3138	252	0.2628	4803	X-RAY DIFFRACTION	99.86
2.05-2.07	0.2928	281	0.2522	4786	X-RAY DIFFRACTION	99.88
2.07-2.1	0.2791	250	0.2517	4851	X-RAY DIFFRACTION	99.86
2.1-2.13	0.307	276	0.2447	4803	X-RAY DIFFRACTION	99.86
2.13-2.16	0.2888	265	0.251	4797	X-RAY DIFFRACTION	99.78
2.16-2.2	0.2727	246	0.2285	4841	X-RAY DIFFRACTION	99.76
2.2-2.23	0.259	229	0.2359	4849	X-RAY DIFFRACTION	99.8
2.23-2.27	0.3011	261	0.2314	4779	X-RAY DIFFRACTION	99.86
2.27-2.31	0.2436	296	0.2255	4769	X-RAY DIFFRACTION	99.69
2.31-2.36	0.2844	261	0.2215	4877	X-RAY DIFFRACTION	99.81
2.36-2.4	0.2611	261	0.2271	4826	X-RAY DIFFRACTION	99.71
2.4-2.46	0.2861	236	0.2345	4810	X-RAY DIFFRACTION	99.66
2.46-2.51	0.2522	269	0.2292	4803	X-RAY DIFFRACTION	99.69
2.51-2.58	0.2945	253	0.2272	4844	X-RAY DIFFRACTION	99.53
2.58-2.65	0.2504	238	0.2166	4802	X-RAY DIFFRACTION	99.45
2.65-2.72	0.2602	247	0.2182	4857	X-RAY DIFFRACTION	99.59
2.72-2.81	0.2611	245	0.2277	4817	X-RAY DIFFRACTION	99.7
2.81-2.91	0.2857	251	0.237	4833	X-RAY DIFFRACTION	99.61
2.91-3.03	0.2632	257	0.2324	4845	X-RAY DIFFRACTION	99.11
3.03-3.17	0.2798	258	0.2299	4752	X-RAY DIFFRACTION	98.84
3.17-3.33	0.2557	267	0.2225	4794	X-RAY DIFFRACTION	98.69
3.33-3.54	0.2396	265	0.2174	4779	X-RAY DIFFRACTION	98.52
3.54-3.82	0.225	251	0.1995	4804	X-RAY DIFFRACTION	98.83
3.82-4.2	0.2164	238	0.1799	4842	X-RAY DIFFRACTION	98.76
4.2-4.81	0.1615	226	0.1609	4815	X-RAY DIFFRACTION	98.32
4.81-6.05	0.2143	255	0.1725	4789	X-RAY DIFFRACTION	97.28
6.05-35.82	0.1781	228	0.162	4749	X-RAY DIFFRACTION	94.57

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.0171180080995	0.0638383178174	0.177386707696	0.51061143471	1.41866966662	4.01897275746	0.686125705317	-0.0279920757585	-0.203670060606	0.196697331624	-0.0826364360983	-0.115216489358	1.11763956816	0.0614496302119	-0.61017922309	1.08526780216	0.0602778200949	-0.145691307395	1.31839178164	0.0474549245992	0.585097503257	46.734581298	23.5233753816	29.9197207234
2	0.0293757916492	-0.0257057178974	0.164498570301	0.061494877769	-0.376176187563	2.01027297778	0.483873478056	0.138356469615	-0.175941628448	-0.14155258022	-0.187949860827	0.180729902106	1.2264646315	0.161207526152	-0.276931392269	1.11393484235	0.0945778577133	-0.104882107661	1.32571021981	-0.0344703501717	0.595479855006	45.2503486009	23.4465848686	25.6014161782
3	7.84437839373	-0.115261000127	0.660498998132	4.90707320069	0.518878612022	4.58065139982	0.185443658933	-0.657225363558	0.0830985911567	0.411130411383	0.0965527803569	0.848666802583	-0.331325488637	-0.705357818436	-0.255410739747	0.331625736354	0.0594431049151	0.125831985687	0.361819537956	0.0869069413433	0.306442274742	-21.0073516788	18.2489417616	18.8463126668
4	5.51464230307	1.41175089964	-0.235972971088	3.14367433503	-0.114414020568	2.82460634273	0.0907324107481	0.0981718115899	-0.29134475855	-0.157547853114	0.0533499141515	0.314864052863	0.0883353504819	-0.291517122118	-0.141063274574	0.210077192062	0.0368176680564	-0.00456470385716	0.172188468572	0.0109106015141	0.206316818924	-13.1024925711	10.8029093998	6.57494079618
5	2.84975535977	-0.203036436105	-0.346914039366	3.7436912336	0.918554039032	2.40506554503	0.0741054282464	-0.183455998223	0.228510603149	-0.0709656319756	0.0379163352052	-0.429862184612	-0.309287584065	0.527543493906	-0.110166519507	0.247998051387	-0.0739979634056	0.0483102946372	0.307072766526	0.0322458731325	0.216270656661	11.0332922532	15.5106584567	13.767331488
6	4.49740949352	0.118224418578	-0.726841498186	2.23661733083	-0.218850858726	2.22798124195	0.229111161024	0.165181353216	0.445634339803	-0.145642674827	0.0283065409474	0.283774508901	-0.464140582655	-0.249375315578	-0.254395834866	0.319414625878	0.03722484647	0.0214967012527	0.205246383429	0.0553111847918	0.181559207828	-10.4803494361	20.7304297898	6.53333188545
7	2.95749829757	-0.638868438554	0.438200248482	2.26889995887	1.12929886794	1.86069408486	0.09649789494	-0.311883485083	-0.353227095624	-0.121576253598	-0.0742410095364	-0.0311047588466	0.303900562055	0.208120563795	-0.0228008328322	0.205266707653	-0.00365057399167	0.0708406043715	0.245029179683	0.0562954679944	0.210672421497	3.72956285355	3.73847434952	9.77543334058
8	3.16891542361	-2.72141116898	1.66346875745	8.55234420144	-7.86977535113	9.73461746818	0.114396266672	-0.0968809188634	-0.104216857563	-0.401696413955	0.155083432151	0.137726521309	0.659596424409	-0.226422305379	-0.230912334443	0.204168009743	-0.0370159979975	-0.015330717427	0.159282157376	-0.0140502331847	0.147899183144	-13.2287485394	-29.581096729	22.2079892394
9	3.74974557435	-1.38230735962	0.1055537848	6.04636956203	2.17157812293	1.31832135292	-0.127441854368	-0.667350155729	-0.846057738616	0.633251593452	0.203030964964	-0.196987581692	1.10678846039	0.274201720533	-0.06985587074	0.82664218182	0.116152548417	-0.0350026323219	0.303125555164	0.121148264608	0.467721297025	-1.49060843884	-44.4242927337	34.7233951452
10	3.40059159352	0.770127810351	0.0446813927016	1.33464570762	-0.129044943149	1.86603899765	0.0318158302871	0.177954988343	0.0234445770295	0.102351826727	0.0783368421895	-0.00862283862438	-0.117369794285	-0.19967963843	-0.102168906875	0.276483911863	0.0512542435281	0.0110843870204	0.226521311624	-0.00331064107153	0.151402295181	-8.02175009458	14.0341013581	44.5553907523
11	2.5964023804	1.26255966497	0.607198163006	3.32381436849	0.952074512776	2.94355901116	0.105264214632	0.0658573608687	-0.3398019517	-0.10967490287	0.114538240496	-0.131714156208	0.836990775763	0.0317936345711	-0.199340906253	0.429738363036	0.0307741176619	-0.0285507410544	0.190406039201	0.0542640700117	0.253193139887	-2.62948310247	-37.2874850677	26.5441473855
12	0.0707486288492	-0.0436255634141	-0.795609256107	0.266930160922	0.0975829745621	2.04191312008	0.111920753611	-0.0746090357922	-0.0195063953227	0.129141697729	0.0639228977281	0.00945789515342	0.270956658912	-0.577622828719	-0.2620596926	0.35409698275	-0.0160912041386	-0.0117554437318	0.308900229923	0.0190601367205	0.220651328761	-6.62972926655	-24.3982308142	29.5225613692
13	1.0581200131	-0.393046839895	-2.32977415822	1.90833117609	2.64811267409	8.80475437605	0.143342314533	-0.324370498522	0.0236136533046	0.0338273268715	0.224891543305	-0.142282485143	0.0821366967306	0.830281210886	-0.356046016032	0.23865588462	-0.0202765120857	-0.0391103432658	0.229356951094	-0.0212936720539	0.202972377432	-3.32342422242	-24.4268680059	25.677095624
14	5.40537685526	-0.105608132657	-0.129091748279	0.896110544092	0.0158784617619	0.765945670811	0.133886418569	0.321964000157	0.291263572105	-0.0439819366501	-0.155021081432	0.0225899338506	-0.0664948299793	0.148989410016	0.0243287905868	0.248675589639	0.0633673681585	0.0190425184077	0.285087357053	0.037070381631	0.175337011667	39.6992595608	-13.9228330471	44.2445398144
15	2.02067765451	-1.06749623492	0.689505455092	2.64938613478	-2.93280739351	3.39910162366	-0.0886310465151	0.496737178799	-0.142215094545	0.10981679841	0.350990466518	0.227525405441	-0.624256573613	-0.49274338989	-0.26593925798	0.751153216166	0.0165480339688	0.00374433744338	0.721089668586	-0.119758053324	0.489087307729	45.4827403523	29.1245377885	29.8262637893
16	3.4851400263	-0.00275758706859	-0.530357346324	1.21549916601	-0.185575184531	2.33851660291	0.0255952111125	0.161670484659	0.0729052328852	-0.0782557131848	0.00445985101585	0.203480103577	0.155049814577	-0.937153531329	-0.0283120815066	0.21617210482	-0.0995876479014	-0.0134392621003	0.595595562669	0.00812879560869	0.208330440244	53.0325644846	-15.4909228828	11.3599570409
17	1.31862060048	1.18673266033	0.596642744982	1.0958041175	0.458587319327	1.4428497608	0.0559486590724	-0.288199150699	-0.223311292492	0.509262242699	0.0879628658207	-0.252202824076	-0.368835195426	0.302897703253	-0.121457624768	0.853707086673	0.134136221625	0.0180787553959	1.00603979764	0.198726414003	0.598773692109	46.5889344587	28.4961442991	25.7431109288

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'E' and (resid -3 through 11 )	E	K	-3 - 11
2	2	chain 'F' and (resid -3 through 11 )	F	L	-3 - 11
3	3	chain 'C' and (resid -348 through -306 )	C	A	-348 - -306	1 - 43
4	4	chain 'C' and (resid -305 through -230 )	C	A	-305 - -230	44 - 119
5	5	chain 'C' and (resid -229 through -103 )	C	A	-229 - -103	120 - 246
6	6	chain 'C' and (resid -102 through -34 )	C	A	-102 - -34	247 - 315
7	7	chain 'C' and (resid -33 through 26 )	C	A	-33 - 26	316 - 375
8	8	chain 'C' and (resid 27 through 54 )	C	A	27 - 54	376 - 403
9	9	chain 'C' and (resid 55 through 88 )	C	A	55 - 88	404 - 437
10	10	chain 'D' and (resid -348 through 26 )	D	C	-348 - 26	1 - 375
11	11	chain 'D' and (resid 27 through 88 )	D	C	27 - 88	376 - 437
12	12	chain 'G' and (resid -3 through 11 )	G	E	-3 - 11
13	13	chain 'H' and (resid -3 through 11 )	H	F	-3 - 11
14	14	chain 'A' and (resid -347 through 4 )	A	G	-347 - 4	1 - 352
15	15	chain 'A' and (resid 5 through 88 )	A	G	5 - 88	353 - 436
16	16	chain 'B' and (resid -347 through 3 )	B	I	-347 - 3	1 - 351
17	17	chain 'B' and (resid 4 through 88 )	B	I	4 - 88	352 - 436

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