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- PDB-7tzb: Crystal structure of the human mitochondrial seryl-tRNA synthetas... -
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Basic information
Entry | Database: PDB / ID: 7tzb | ||||||
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Title | Crystal structure of the human mitochondrial seryl-tRNA synthetase (mt SerRS) bound with a seryl-adenylate analogue | ||||||
![]() | Serine--tRNA ligase, mitochondrial | ||||||
![]() | LIGASE / Seryl-tRNA synthetase / mitochondria / aminoacylation / translation / tRNA / class II / alpha-beta domain | ||||||
Function / homology | ![]() mitochondrial seryl-tRNA aminoacylation / Mitochondrial tRNA aminoacylation / seryl-tRNA aminoacylation / serine-tRNA ligase / serine-tRNA ligase activity / tRNA binding / mitochondrial matrix / mitochondrion / RNA binding / ATP binding / cytosol Similarity search - Function | ||||||
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![]() | Kuhle, B. / Hirschi, M. / Doerfel, L. / Lander, G. / Schimmel, P. | ||||||
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![]() | ![]() Title: Structural basis for shape-selective recognition and aminoacylation of a D-armless human mitochondrial tRNA. Authors: Bernhard Kuhle / Marscha Hirschi / Lili K Doerfel / Gabriel C Lander / Paul Schimmel / ![]() Abstract: Human mitochondrial gene expression relies on the specific recognition and aminoacylation of mitochondrial tRNAs (mtRNAs) by nuclear-encoded mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs). ...Human mitochondrial gene expression relies on the specific recognition and aminoacylation of mitochondrial tRNAs (mtRNAs) by nuclear-encoded mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs). Despite their essential role in cellular energy homeostasis, strong mutation pressure and genetic drift have led to an unparalleled sequence erosion of animal mtRNAs. The structural and functional consequences of this erosion are not understood. Here, we present cryo-EM structures of the human mitochondrial seryl-tRNA synthetase (mSerRS) in complex with mtRNA. These structures reveal a unique mechanism of substrate recognition and aminoacylation. The mtRNA is highly degenerated, having lost the entire D-arm, tertiary core, and stable L-shaped fold that define canonical tRNAs. Instead, mtRNA evolved unique structural innovations, including a radically altered T-arm topology that serves as critical identity determinant in an unusual shape-selective readout mechanism by mSerRS. Our results provide a molecular framework to understand the principles of mito-nuclear co-evolution and specialized mechanisms of tRNA recognition in mammalian mitochondrial gene expression. | ||||||
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 34.1 KB | Display | |
Data in CIF | ![]() | 46 KB | Display | |
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-Related structure data
Related structure data | ![]() 7u2aC ![]() 7u2bC ![]() 1wleS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
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