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Yorodumi- PDB-7sus: Crystal structure of Apelin receptor in complex with small molecule -
+Open data
-Basic information
Entry | Database: PDB / ID: 7sus | ||||||
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Title | Crystal structure of Apelin receptor in complex with small molecule | ||||||
Components | Apelin receptor, with Rubredoxin insertion | ||||||
Keywords | MEMBRANE PROTEIN / GPCR / Class A GPCR / small molecule / Apelin receptor | ||||||
Function / homology | Function and homology information apelin receptor activity / apelin receptor signaling pathway / regulation of gap junction assembly / positive regulation of inhibitory G protein-coupled receptor phosphorylation / vascular associated smooth muscle cell differentiation / atrioventricular valve development / regulation of body fluid levels / venous blood vessel development / alkane catabolic process / positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis ...apelin receptor activity / apelin receptor signaling pathway / regulation of gap junction assembly / positive regulation of inhibitory G protein-coupled receptor phosphorylation / vascular associated smooth muscle cell differentiation / atrioventricular valve development / regulation of body fluid levels / venous blood vessel development / alkane catabolic process / positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / endocardial cushion formation / C-C chemokine receptor activity / coronary vasculature development / C-C chemokine binding / adult heart development / vasculature development / negative regulation of cAMP-mediated signaling / aorta development / ventricular septum morphogenesis / blood vessel development / heart looping / vasculogenesis / gastrulation / Peptide ligand-binding receptors / neurogenesis / cell chemotaxis / positive regulation of release of sequestered calcium ion into cytosol / G protein-coupled receptor activity / calcium-mediated signaling / brain development / positive regulation of angiogenesis / signaling receptor activity / heart development / G alpha (i) signalling events / positive regulation of cytosolic calcium ion concentration / regulation of gene expression / angiogenesis / electron transfer activity / iron ion binding / immune response / G protein-coupled receptor signaling pathway / external side of plasma membrane / negative regulation of gene expression / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Clostridium pasteurianum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Xu, F. / Yue, Y. / Liu, L.E. / Han, G.W. / Hanson, M. | ||||||
Funding support | 1items
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Citation | Journal: Nat Struct Mol Biol / Year: 2022 Title: Structural insight into apelin receptor-G protein stoichiometry. Authors: Yang Yue / Lier Liu / Li-Jie Wu / Yiran Wu / Ling Wang / Fei Li / Junlin Liu / Gye-Won Han / Bo Chen / Xi Lin / Rebecca L Brouillette / Émile Breault / Jean-Michel Longpré / Songting Shi / ...Authors: Yang Yue / Lier Liu / Li-Jie Wu / Yiran Wu / Ling Wang / Fei Li / Junlin Liu / Gye-Won Han / Bo Chen / Xi Lin / Rebecca L Brouillette / Émile Breault / Jean-Michel Longpré / Songting Shi / Hui Lei / Philippe Sarret / Raymond C Stevens / Michael A Hanson / Fei Xu / Abstract: The technique of cryogenic-electron microscopy (cryo-EM) has revolutionized the field of membrane protein structure and function with a focus on the dominantly observed molecular species. This report ...The technique of cryogenic-electron microscopy (cryo-EM) has revolutionized the field of membrane protein structure and function with a focus on the dominantly observed molecular species. This report describes the structural characterization of a fully active human apelin receptor (APJR) complexed with heterotrimeric G protein observed in both 2:1 and 1:1 stoichiometric ratios. We use cryo-EM single-particle analysis to determine the structural details of both species from the same sample preparation. Protein preparations, in the presence of the endogenous peptide ligand ELA or a synthetic small molecule, both demonstrate these mixed stoichiometric states. Structural differences in G protein engagement between dimeric and monomeric APJR suggest a role for the stoichiometry of G protein-coupled receptor- (GPCR-)G protein coupling on downstream signaling and receptor pharmacology. Furthermore, a small, hydrophobic dimer interface provides a starting framework for additional class A GPCR dimerization studies. Together, these findings uncover a mechanism of versatile regulation through oligomerization by which GPCRs can modulate their signaling. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7sus.cif.gz | 159.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7sus.ent.gz | 122.2 KB | Display | PDB format |
PDBx/mmJSON format | 7sus.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7sus_validation.pdf.gz | 882 KB | Display | wwPDB validaton report |
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Full document | 7sus_full_validation.pdf.gz | 887.1 KB | Display | |
Data in XML | 7sus_validation.xml.gz | 9.8 KB | Display | |
Data in CIF | 7sus_validation.cif.gz | 13.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/su/7sus ftp://data.pdbj.org/pub/pdb/validation_reports/su/7sus | HTTPS FTP |
-Related structure data
Related structure data | 7w0lC 7w0mC 7w0nC 7w0oC 7w0pC 1iroS 5vblS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The authors state that the biological oligomeric state is unknown. |
-Components
#1: Protein | Mass: 46436.613 Da / Num. of mol.: 1 / Mutation: V117A,E174C,T177N,M217C,I250C,C325L,C326M Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clostridium pasteurianum (bacteria) Gene: APLNR, AGTRL1, APJ / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P35414, UniProt: P00268 |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-8EH / ( |
#4: Chemical | ChemComp-OLC / ( |
#5: Water | ChemComp-HOH / |
Has ligand of interest | Y |
Sequence details | Rubredoxin from a Clostridium species is inserted in the cytoplasmic region between helices 5 and 6 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.22 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 100 mM MES pH 6.1, 26% PEG500 DME, 125 mM MgCl2, 100 mM NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 19, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→42.93 Å / Num. obs: 13301 / % possible obs: 92.3 % / Redundancy: 4.4 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2.7→2.85 Å / Rmerge(I) obs: 0.56 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 1636 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5VBL, 1IRO Resolution: 2.7→30 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.891 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.755 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.697 / SU Rfree Blow DPI: 0.335 / SU Rfree Cruickshank DPI: 0.344
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Displacement parameters | Biso max: 274.36 Å2 / Biso mean: 129.6 Å2 / Biso min: 30 Å2
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Refinement step | Cycle: final / Resolution: 2.7→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.92 Å / Rfactor Rfree error: 0 / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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