温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Best crystals of aureolysin in complex with IMPI mutant I57F were obtained at 20 dgrees with protein solutions consisting of 5 mg/mL of aureolysin and 2.9 mg/mL of IMPI (peptidase:inhibitor ...詳細: Best crystals of aureolysin in complex with IMPI mutant I57F were obtained at 20 dgrees with protein solutions consisting of 5 mg/mL of aureolysin and 2.9 mg/mL of IMPI (peptidase:inhibitor molar ratio of 1:2.5) in 50 mM Tris-HCl, 150 mM sodium chloride, 1.6 mM calcium chloride, 8.3 microM zinc chloride, pH 8.0, which was mixed with reservoir solution comprising 0.1 M Bis-Tris, 25% (w/v) PEG 3350, pH 5.5. PH範囲: 5.5
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データ収集
回折
平均測定温度: 100 K / Serial crystal experiment: N
放射光源
由来: シンクロトロン / サイト: ALBA / ビームライン: XALOC / 波長: 0.9793 Å
解像度: 1.6→68.08 Å / Cor.coef. Fo:Fc: 0.976 / Cor.coef. Fo:Fc free: 0.972 / SU B: 2.656 / SU ML: 0.049 / 交差検証法: FREE R-VALUE / ESU R: 0.016 / ESU R Free: 0.016 / 詳細: Hydrogens have not been used
Rfactor
反射数
%反射
Rfree
0.1878
681
0.687 %
Rwork
0.1579
98470
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all
0.158
-
-
obs
-
99151
99.706 %
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL PLUS MASK