PDB-7rth: Crystal structure of an anti-lysozyme nanobody in complex with an...

Basic information

• Entry: Database: PDB / ID: 7rth
• Title: Crystal structure of an anti-lysozyme nanobody in complex with an anti-nanobody Fab "NabFab"

Components

• (Fragment Antigen-Binding ...) x 2
• Nanobody

• Keywords: IMMUNE SYSTEM / Antigen / FAB / Single-Domain Antibody
• Function / homology: DI(HYDROXYETHYL)ETHER / PHOSPHATE ION
• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.19 Å
• Authors: Filippova, E.V. / Mukherjee, S. / Bloch, J.S. / Locher, K.P. / Kossiakoff, A.A.

Funding support

United States, 1items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)		United States

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2021; Title: Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.; Authors: Joël S Bloch / Somnath Mukherjee / Julia Kowal / Ekaterina V Filippova / Martina Niederer / Els Pardon / Jan Steyaert / Anthony A Kossiakoff / Kaspar P Locher / ; Abstract: With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a demand for antigen-binding fragments (Fabs) that specifically and tightly bind these nanobodies without disturbing the nanobody-target protein interaction. Here, we describe the development of a synthetic Fab (termed NabFab) that binds the scaffold of an alpaca-derived nanobody with picomolar affinity. We demonstrate that upon complementary-determining region grafting onto this parent nanobody scaffold, nanobodies recognizing diverse target proteins and derived from llama or camel can cross-react with NabFab without loss of affinity. Using NabFab as a fiducial and size enhancer (50 kDa), we determined the high-resolution cryogenic electron microscopy (cryo-EM) structures of nanobody-bound VcNorM and ScaDMT, both small membrane proteins of ∼50 kDa. Using an additional anti-Fab nanobody further facilitated reliable initial three-dimensional structure determination from small cryo-EM test datasets. Given that NabFab is of synthetic origin, is humanized, and can be conveniently expressed in in large amounts, it may be useful not only for structural biology but also for biomedical applications.

History

Deposition	Aug 13, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Sep 1, 2021	Provider: repository / Type: Initial release
Revision 1.1	Mar 16, 2022	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Oct 18, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.3	Nov 13, 2024	Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

Assembly

Deposited unit

A: Fragment Antigen-Binding Light Chain

B: Fragment Antigen-Binding Heavy Chain

C: Fragment Antigen-Binding Light Chain

D: Fragment Antigen-Binding Heavy Chain

E: Fragment Antigen-Binding Light Chain

F: Fragment Antigen-Binding Heavy Chain

G: Fragment Antigen-Binding Light Chain

H: Fragment Antigen-Binding Heavy Chain

I: Fragment Antigen-Binding Light Chain

J: Fragment Antigen-Binding Heavy Chain

K: Fragment Antigen-Binding Light Chain

L: Fragment Antigen-Binding Heavy Chain

M: Fragment Antigen-Binding Light Chain

N: Fragment Antigen-Binding Heavy Chain

O: Fragment Antigen-Binding Light Chain

P: Fragment Antigen-Binding Heavy Chain

Q: Fragment Antigen-Binding Light Chain

R: Fragment Antigen-Binding Heavy Chain

a: Nanobody

c: Nanobody

e: Nanobody

g: Nanobody

i: Nanobody

k: Nanobody

m: Nanobody

o: Nanobody

q: Nanobody

hetero molecules

Summary:

• 575 kDa, 27 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	574,950	118
Polymers	568,026	27
Non-polymers	6,924	91
Water	2,972	165

1

A: Fragment Antigen-Binding Light Chain

B: Fragment Antigen-Binding Heavy Chain

a: Nanobody

hetero molecules

Summary:

• defined by author
• Evidence: gel filtration
• 63.6 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	63,562	9
Polymers	63,114	3
Non-polymers	448	6
Water	54	3

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

C: Fragment Antigen-Binding Light Chain

D: Fragment Antigen-Binding Heavy Chain

E: Fragment Antigen-Binding Light Chain

F: Fragment Antigen-Binding Heavy Chain

c: Nanobody

e: Nanobody

hetero molecules

Summary:

• defined by author
• 128 kDa, 6 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	128,414	34
Polymers	126,228	6
Non-polymers	2,186	28
Water	108	6

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

3

G: Fragment Antigen-Binding Light Chain

H: Fragment Antigen-Binding Heavy Chain

I: Fragment Antigen-Binding Light Chain

J: Fragment Antigen-Binding Heavy Chain

g: Nanobody

i: Nanobody

hetero molecules

Summary:

• defined by author
• 128 kDa, 6 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	128,283	32
Polymers	126,228	6
Non-polymers	2,055	26
Water	108	6

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

4

K: Fragment Antigen-Binding Light Chain

L: Fragment Antigen-Binding Heavy Chain

M: Fragment Antigen-Binding Light Chain

N: Fragment Antigen-Binding Heavy Chain

k: Nanobody

m: Nanobody

hetero molecules

Summary:

• defined by author
• 127 kDa, 6 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	127,491	23
Polymers	126,228	6
Non-polymers	1,263	17
Water	108	6

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

5

O: Fragment Antigen-Binding Light Chain

P: Fragment Antigen-Binding Heavy Chain

Q: Fragment Antigen-Binding Light Chain

R: Fragment Antigen-Binding Heavy Chain

o: Nanobody

q: Nanobody

hetero molecules

Summary:

• defined by author
• 127 kDa, 6 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	127,201	20
Polymers	126,228	6
Non-polymers	972	14
Water	108	6

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	225.530, 130.280, 223.980
Angle α, β, γ (deg.)	90.000, 107.990, 90.000
Int Tables number	5
Space group name H-M	C121

Components

Antibody , 3 types, 27 molecules A C E G I K M O Q B D F H J L N P R a c e g i k m o q

#1: Antibody

A C E G I K M O Q
Fragment Antigen-Binding Light Chain

Details:

Mass: 23258.783 Da / Num. of mol.: 9
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pRH2.2 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): C43

#2: Antibody

B D F H J L N P R
Fragment Antigen-Binding Heavy Chain

Details:

Mass: 25684.463 Da / Num. of mol.: 9
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pRH2.2 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): C43

#3: Antibody

a c e g i k m o q
Nanobody

Details:

Mass: 14170.781 Da / Num. of mol.: 9
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET26b+ / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3)

Non-polymers , 5 types, 256 molecules

#4: Chemical

B-301 C-301 C-302 C-303 C-304 C-305 D-301 D-302 E-301 E-302 E-303 E-304 G-301 G-302 G-303 H-301 I-301 I-302 I-303 I-304 ...
ChemComp-PO4 / PHOSPHATE ION

Details:

Mass: 94.971 Da / Num. of mol.: 50 / Source method: obtained synthetically / Formula: PO₄ / Feature type: SUBJECT OF INVESTIGATION

#5: Chemical

B-302 B-303 C-306 C-307 D-303 D-304 E-305 E-306 F-301 G-304 G-305 H-302 I-305 J-303 K-303 K-304 L-302 M-304 N-301 N-302 ...
ChemComp-CL / CHLORIDE ION

Details:

Mass: 35.453 Da / Num. of mol.: 29 / Source method: obtained synthetically / Formula: Cl / Feature type: SUBJECT OF INVESTIGATION

#6: Chemical

B-304 C-308 E-307 G-306 G-307 I-306 L-303 c-203 k-201
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL

Details:

Mass: 92.094 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C₃H₈O₃

#7: Chemical

F-302 H-303 N-303 ChemComp-PEG / DI(HYDROXYETHYL)ETHER

Details:

Mass: 106.120 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₄H₁₀O₃

#8: Water

ChemComp-HOH / water

Details:

Mass: 18.015 Da / Num. of mol.: 165 / Source method: isolated from a natural source / Formula: H₂O

Details

• Has ligand of interest: Y
• Has protein modification: Y

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.92 ų/Da / Density % sol: 57.53 %
• Crystal grow: Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 8.8 ; Details: 0.02 M Citric acid, 0.08 M BIS-TRIS propane, 16 % (w/v) PEG3350

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
• Detector: Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Jun 11, 2021 / Details: 3.3 Undulator (Undulator A)
• Radiation: Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.97918 Å / Relative weight: 1
• Reflection: Resolution: 3.19→111.43 Å / Num. obs: 102005 / % possible obs: 99.4 % / Redundancy: 5.7 % / CC_1/2: 0.98 / R_pim(I) all: 0.17 / Net I/σ(I): 9.5
• Reflection shell: Resolution: 3.19→3.36 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 1.9 / Num. unique obs: 14566 / CC_1/2: 0.47 / R_pim(I) all: 0.737

Phasing

• Phasing: Method: molecular replacement

Processing

Software

Name	Version	Classification	NB
PHENIX	1.16_3549	refinement
XDS		data reduction
Aimless		data scaling
PHASER		phasing
PDB_EXTRACT	3.27	data extraction

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 6JB9, 5C8J

6jb9

5c8j

Resolution: 3.19→90.907 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 0.31 / Phase error: 26.41 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2671	5099	5.12 %
Rwork	0.2159	94559	-
obs	0.2185	99658	96.98 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 249.68 Ų / B_iso mean: 77.104 Ų / B_iso min: 7.32 Ų

Refinement step

Cycle: final / Resolution: 3.19→90.907 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	37922	0	354	165	38441
Biso mean	-	-	82.41	30.83	-
Num. residues	-	-	-	-	4958

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	% reflection obs (%)
3.1905-3.2267	0.3091	65		1411	44
3.2267-3.2647	0.3235	141	0.3	2586	79
3.2647-3.3045	0.3431	161	0.2985	3091	97
3.3045-3.3463	0.3435	170	0.2829	3304	100
3.3463-3.3904	0.3391	169	0.2807	3187	100
3.3904-3.4368	0.3323	179	0.2716	3269	100
3.4368-3.4859	0.31	159	0.2617	3181	100
3.4859-3.538	0.2856	192	0.2557	3251	100
3.538-3.5932	0.2777	182	0.2547	3224	100
3.5932-3.6522	0.3054	166	0.2602	3225	100
3.6522-3.7151	0.3178	186	0.2538	3242	100
3.7151-3.7827	0.3043	181	0.2499	3212	100
3.7827-3.8554	0.3332	178	0.2359	3211	100
3.8554-3.9341	0.2895	141	0.2344	3298	100
3.9341-4.0197	0.2551	200	0.2263	3169	100
4.0197-4.1132	0.2759	178	0.213	3270	100
4.1132-4.2161	0.2805	192	0.2135	3211	100
4.2161-4.33	0.2309	153	0.1904	3276	100
4.33-4.4575	0.2502	194	0.1822	3175	99
4.4575-4.6013	0.2346	166	0.1704	3246	100
4.6013-4.7658	0.2065	150	0.1715	3216	99
4.7658-4.9566	0.2083	170	0.1699	3202	98
4.9566-5.1821	0.2302	201	0.1723	3238	100
5.1821-5.4553	0.2553	164	0.1749	3264	100
5.4553-5.7971	0.2749	185	0.1886	3239	100
5.7971-6.2446	0.2528	197	0.1985	3266	100
6.2446-6.8728	0.2324	161	0.2097	3280	100
6.8728-7.8668	0.2607	172	0.2053	3221	99
7.8668-9.9094	0.2105	173	0.1781	3269	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.3962	1.853	0.9557	3.0445	1.6553	0.912	0.4018	0.1657	0.0545	0.3063	0.2217	-0.4416	-0.0808	-0.0049	0.1335	1.983	0.4869	0.6487	0.3918	0.0356	0.7975	20.7653	72.3314	-98.2239
2	3.259	0.7803	0.5954	4.2305	-2.2239	3.4355	0.4024	0.285	0.5813	0.497	0.0801	0.1269	-0.5547	-0.1144	-0.0771	1.4335	0.0405	0.227	0.2947	0.2673	0.4955	19.2835	61.725	-92.3417
3	0.8433	0.6159	-0.2955	2.0241	-0.2644	0.1048	0.2462	0.4414	0.7158	0.5927	0.1159	0.7118	-0.6379	-0.3316	-0.2295	1.3324	0.3457	0.315	0.6834	0.3645	0.6718	9.6717	64.7204	-94.0264
4	2.2229	-0.9548	-0.0877	2.5199	0.1414	1.246	0.815	0.1622	0.7154	0.6037	-0.2452	0.3011	-0.5204	-0.2282	0.252	1.863	0.4808	0.6266	0.6403	0.1975	0.7432	18.7587	68.5889	-92.0902
5	0.5711	-0.0015	0.0292	1.0803	-0.7222	0.4834	0.377	-0.081	0.8004	0.0724	0.4388	0.1652	-1.2283	0.1649	-0.1052	1.7507	-0.1076	0.6039	0.6482	-0.1861	0.782	27.0934	70.6939	-97.4061
6	1.6861	0.1475	-1.0892	0.0144	-0.08	0.9234	-0.1189	-0.0902	-0.2381	0.1423	0.0895	-0.14	0.0726	0.3402	0.3693	2.1025	-1.1976	0.7085	1.755	-0.625	1.1601	61.1691	76.0503	-92.8613
7	0.3734	0.1691	0.3349	1.0785	0.7027	0.5716	0.1643	0.124	-0.0404	0.0742	-0.0213	-0.0834	-0.4857	0.2605	0.0404	2.5398	-0.8358	0.6622	0.4716	-0.6726	0.8722	45.8428	79.1579	-100.0594
8	1.7603	-0.1035	-1.2722	0.0739	-0.2434	2.5394	-0.105	0.0312	0.0436	0.3265	0.3151	-0.0768	-0.6022	0.1849	-0.0811	1.9705	-0.5529	0.5423	0.8349	-0.3209	0.7276	43.5007	76.8492	-97.9911
9	3.0241	0.3067	0.27	0.9804	0.6172	1.2326	0.0544	0.215	-0.2776	-0.4449	0.3962	-0.5006	-0.5267	0.858	0.065	1.6846	-1.1525	0.4095	1.1878	-0.4394	1.1195	55.4131	73.9973	-99.4855
10	3.0841	0.5415	1.3091	1.0892	0.7471	1.6511	0.213	0.3153	-0.2729	-0.0171	0.1678	-0.391	-0.0893	0.4935	-0.393	2.0669	-0.4557	0.7435	0.512	-0.5098	0.878	54.7031	81.8439	-104.9752
11	3.9675	1.0958	-1.7045	0.4151	-0.4581	1.1389	-0.0341	0.1199	-0.0952	-0.3879	0.1921	-0.3546	-0.5378	0.28	0.0417	2.5107	-0.6052	0.9918	0.8884	-0.2257	1.1038	50.0165	87.3358	-102.9118
12	4.7344	0.9928	-1.0105	2.9047	-0.6277	4.1611	0.7251	-0.3939	-0.4015	0.8581	-0.441	-0.6805	-0.2511	0.2778	-0.2853	0.7775	-0.1396	-0.1072	0.3713	0.0919	0.7434	27.974	46.8236	-85.0468
13	3.085	0.1059	1.5224	6.0822	5.214	7.0508	0.4985	-0.1495	-0.9154	0.4919	-0.0447	-0.1811	0.0373	0.5293	-0.2463	0.6632	0.0877	-0.0089	0.387	0.0916	0.5844	26.473	45.0331	-96.0576
14	2.7309	0.1997	-0.1899	0.9348	-0.2346	1.2071	-0.2083	-0.0352	-0.4122	0.2535	0.0765	-0.4168	-0.2267	0.0888	0.1472	0.9025	-0.0329	-0.0092	0.5159	0.1343	0.7179	23.8803	47.8266	-90.311
15	1.5012	0.7931	1.3288	0.994	1.5493	2.5366	0.5529	-0.4683	0.1429	1.1053	0.145	-0.1804	-0.991	1.0169	-0.0844	2.1449	-0.7156	0.4668	1.5151	-0.4364	0.7636	50.0712	70.8001	-86.2764
16	0.3943	-0.0614	-0.3262	0.0193	0.0421	0.266	0.0633	-0.0036	0.0403	-0.0571	-0.1571	0.4285	0.0295	0.2417	0.0663	1.4702	-0.2901	-0.095	1.4195	-0.2032	0.566	55.5015	67.0729	-78.0819
17	5.4067	0.6382	-0.3249	2.2551	0.0871	2.1017	0.0638	0.3867	-0.2813	-0.1875	0.0319	0.2235	-0.0479	-0.0386	-0.0727	0.2416	-0.0205	-0.1031	0.2487	0.013	0.4459	-1.1305	88.5664	-25.8545
18	7.0597	0.9663	1.2371	1.2269	0.2313	1.46	-0.1242	0.6687	0.0556	-0.3294	0.3255	0.2167	-0.0818	-0.2096	-0.2612	0.3515	-0.0158	-0.0418	0.2372	0.032	0.3447	-8.3595	95.1202	-28.5732
19	2.2295	-0.2446	0.1554	4.6978	-0.7189	2.1661	-0.2552	-0.0585	0.4402	0.0283	0.1535	-0.0352	-0.15	0.0752	0.0635	0.1207	0.0025	0.0033	0.4365	0.0073	0.4524	-30.1597	110.584	-26.7021
20	0.7776	-0.0877	-1.5128	6.1489	-1.2753	3.3601	-0.1175	0.0305	-0.0491	0.1277	-0.3546	-0.5092	-0.3286	-0.6614	0.2348	0.455	-0.0688	0.0514	0.7581	0.1163	0.5124	-26.7033	114.0527	-34.5788
21	2.1878	-0.5413	0.0634	4.0781	0.195	2.0308	0.2211	-0.2242	0.2848	-0.0861	-0.1449	0.193	-0.291	-0.3478	-0.1047	0.1863	-0.073	0.0512	0.4974	0.0839	0.4707	-31.5147	109.2773	-29.2142
22	3.1883	-0.9289	0.0628	2.2964	-0.1441	3.5569	-0.2179	-0.7429	0.7049	0.7377	-0.3239	0.0762	-0.3069	-0.0014	0.5155	0.381	-0.1756	-0.0387	0.7145	-0.1524	0.8708	3.9663	111.6733	-12.0145
23	0.2778	0.2445	-0.0541	3.4073	0.2984	4.7843	-0.0854	-0.323	0.6311	0.2758	0.1074	-0.1958	0.2728	0.7034	-0.0732	0.2823	-0.0613	-0.0847	0.5558	-0.0631	0.6252	9.5504	104.7475	-15.3137
24	5.0829	2.7013	0.3273	2.8787	0.3401	2.7407	-0.1132	0.4228	0.3552	0.1705	0.0372	-0.3658	-0.4172	0.6708	0.1061	0.2098	-0.1548	-0.118	0.2631	0.0333	0.7857	13.2528	107.8376	-24.6626
25	1.8757	-1.8458	-0.3012	3.6863	0.0533	0.7404	-0.0372	0.2629	0.6486	0.4042	-0.0949	-0.4095	-0.303	0.1642	0.1106	0.2223	-0.0203	-0.0685	0.5653	-0.0571	0.6408	9.9919	105.5988	-19.4832
26	1.2225	-1.6025	-0.5799	2.34	-0.1416	3.2946	-0.42	-0.287	0.5432	-0.4865	0.1799	0.5885	0.0412	-0.0626	0.1915	0.562	-0.0937	0.0246	0.3207	0.0072	0.5718	-32.1936	112.9669	-16.9406
27	2.977	1.1309	-0.0116	3.635	0.026	2.038	0.2354	-0.3636	0.5233	0.5092	-0.1638	0.0205	0.2317	0.1765	-0.0188	0.4397	-0.0349	-0.0059	0.3877	-0.012	0.4276	-22.8438	110.2008	-11.453
28	0.5304	-0.084	0.388	0.7967	0.3781	0.532	0.3444	-0.1756	0.7105	0.0482	-0.2762	-0.2906	-0.4785	-0.1032	-0.0911	0.3726	-0.0685	0.0569	0.3243	0.0423	0.6201	-19.7972	113.6126	-20.1692
29	6.0402	2.5373	-1.3753	2.6323	0.3566	1.2037	-0.4067	-0.1865	-0.3269	0.2862	-0.1917	0.4784	0.3116	-0.2658	0.4043	0.3767	-0.1114	-0.0194	0.3335	0.0347	0.2554	-40.7047	102.2842	-13.0662
30	4.3664	1.6175	-1.8893	4.2803	-0.5752	2.8738	0.4629	-0.4234	0.4131	0.9777	-0.0497	0.1034	0.0685	-0.1878	-0.3743	0.7544	-0.0767	0.015	0.7873	-0.1927	0.4275	-29.9536	114.4433	-7.7905
31	5.4223	-2.2446	-2.2083	3.0494	0.1082	1.3128	0.0375	-0.2837	-0.1074	0.0908	0.2265	-0.2521	0.2421	0.299	-0.2339	0.3191	0.0024	-0.1266	0.6044	0.1899	0.5976	-22.8593	114.8221	-43.2181
32	3.1752	0.2846	0.2344	6.6434	2.697	3.0447	0.3468	-0.4378	-0.2926	0.1571	-0.1243	-0.6219	0.3251	-0.0235	-0.1984	0.3037	-0.0074	-0.0006	0.4576	0.1671	0.4564	-14.8652	124.1768	-47.2745
33	0.4527	0.7572	-0.3075	2.6254	-0.0759	0.7309	0.2008	0.116	-0.2079	0.3342	0.1905	0.1041	0.2038	-0.0399	-0.3586	0.446	0.1383	-0.0952	0.5551	0.0939	0.5283	-21.1358	120.0063	-46.9127
34	5.1302	-2.1793	0.3856	3.2842	-1.1034	1.9183	0.0532	0.5298	0.2036	-0.3304	-0.2438	-0.1236	0.3251	-0.3683	0.1843	0.3916	-0.0421	-0.0742	0.4008	-0.0865	0.3545	-11.6032	86.1701	-47.9997
35	6.0712	-2.9316	2.5953	5.4422	-3.1859	6.9145	0.1086	0.6499	0.2445	-0.2445	-0.0451	0.0044	-0.5098	-0.4304	-0.0786	0.4451	-0.0372	-0.023	0.5071	-0.0325	0.3614	-15.4534	94.7994	-47.6217
36	5.1083	-0.8794	-0.6115	1.6502	-0.3371	1.0854	-0.2052	0.0667	-0.8423	-0.0704	0.2071	0.1207	0.2612	0.0287	0.0245	0.3946	-0.0211	-0.1122	0.3998	-0.0782	0.6529	-11.8012	80.7561	-43.6948
37	0.0452	-0.1585	-0.2085	1.9055	2.2238	2.6503	-0.1002	-0.2604	-0.4702	-0.9742	0.1728	-1.0047	-0.1836	0.6859	-0.0509	0.4982	-0.0319	0.1915	0.5276	0.1374	0.9436	1.6067	124.5519	-57.0715
38	3.9695	1.1609	0.762	5.1054	0.9563	3.2409	-0.1038	-0.1827	0.0449	0.392	-0.1284	-1.0209	-0.0259	0.1524	0.2329	0.3571	-0.0528	-0.0042	0.5346	0.0592	0.863	1.6028	124.6262	-45.3213
39	2.5198	-1.6468	-0.4096	4.9297	0.84	1.6666	-0.1457	-0.2694	-0.0659	0.0231	0.3404	-1.1473	0.0407	0.25	-0.1871	0.3637	-0.0102	-0.0386	0.3965	-0	0.6101	-0.1749	125.0998	-51.2606
40	1.6476	-1.6671	0.6182	2.0623	-0.0353	2.8947	-0.0981	0.4309	-0.0631	-1.0541	-0.1497	0.1379	-0.1003	-0.3141	0.1832	0.7946	-0.0565	-0.0629	0.9062	0.0146	0.3245	-8.8965	84.2779	-58.389
41	3.8039	0.5362	1.1048	3.0745	0.9351	1.8479	-0.107	1.102	0.195	-0.9873	0.0984	-0.2243	0.2226	0.0214	0.017	0.8139	0.0379	-0.1161	0.9711	-0.0016	0.4871	-6.8156	91.0129	-61.7239
42	1.2104	-0.8661	0.772	4.3648	1.4387	1.5585	0.2335	-0.1439	0.3073	-0.4629	-0.3402	0.5634	0.0106	-0.1792	0.1107	0.2804	-0.0269	-0.0604	0.5537	0.11	0.7515	26.3733	28.4288	-29.2895
43	7.5075	-1.9502	-4.2571	4.0004	2.8938	3.7609	0.4505	0.1884	0.5138	-0.0924	-0.1915	-0.061	-0.3992	-0.5484	-0.2951	0.2538	-0.0016	-0.0706	0.3098	0.1475	0.4457	38.2387	30.5195	-24.7399
44	2.3359	-0.9943	0.3844	0.8585	-0.1983	0.0048	0.2002	0.0914	-0.5975	0.015	-0.1337	0.1646	-0.0677	-0.1275	-0.0792	0.3533	0.0224	-0.0557	0.5166	0.1113	0.5	31.4275	27.3069	-25.2736
45	2.0692	0.0651	0.4918	4.121	-0.0534	2.2365	-0.1197	-0.2583	0.1298	0.1756	-0.0369	0.1177	-0.0133	-0.2003	0.1642	0.1681	-0.0463	-0.011	0.4391	0.0332	0.2338	6.6652	51.8026	-26.9273
46	4.6752	-4.1719	1.3791	3.9694	-0.3395	3.5654	-0.137	-0.4614	0.6933	0.5113	0.1565	0.5924	-0.4349	0.0465	-0.0996	0.3337	-0.0164	-0.0857	0.3922	-0.218	0.6897	42.1952	50.1172	-14.0601
47	1.7991	-0.7716	0.1852	3.3585	-0.4272	3.4433	0.2735	-0.4809	0.1501	-0.2969	-0.0076	0.0264	0.1575	0.3706	-0.2471	0.1956	-0.0817	0.0203	0.4064	0.0125	0.3666	46.3275	42.0592	-18.7161
48	4.3532	1.137	-4.7058	7.9256	2.336	6.7519	-0.4895	-0.2516	0.2859	0.4169	-0.3657	-1.4157	0.0301	0.2058	0.6953	0.2858	-0.0041	-0.1743	0.4821	-0.0015	0.468	53.551	43.7608	-26.2452
49	1.4288	-1.527	0.453	1.6114	-0.2748	1.3108	-0.1768	-0.1027	0.4149	0.0563	0.0189	-0.579	-0.2038	0.1608	0.11	0.28	0.0056	-0.0284	0.422	0.0383	0.4554	42.4482	45.2453	-20.3458
50	1.4278	-2.6333	-1.4062	5.2912	1.4536	4.5587	-0.7987	0.1448	-0.1354	-0.1138	0.1629	1.3627	0.479	-1.2231	0.0246	0.5502	-0.0414	0.0145	0.8311	0.2329	0.2894	1.054	51.7364	-15.9833
51	3.3545	-1.1711	-1.1653	3.4212	-1.0555	1.7028	-0.4185	-1.2102	0.327	1.3615	0.4044	-0.3447	-0.6616	-0.6449	-0.0866	0.6389	0.1029	0.075	0.8272	0.0151	0.2123	13.198	54.057	-11.0675
52	1.6171	1.3951	-0.4591	3.506	0.0769	1.1419	0.1956	-0.1312	0.0131	-0.1142	-0.05	-0.1305	-0.0493	0.0695	-0.1451	0.2878	-0.0398	-0.0699	0.4297	-0.0018	0.3578	15.7725	58.6203	-46.4628
53	4.0443	-0.9096	0.3894	3.7573	-0.4739	2.8066	0.0186	0.3221	-0.6427	-0.0925	-0.0219	-0.4923	0.146	0.2369	0.0286	0.2437	-0.0485	-0.0535	0.3025	-0.0998	0.4918	19.2556	24.3	-43.7772
54	3.1387	-1.1993	-0.3673	1.6437	0.9593	3.1758	0.1036	0.1557	-0.5777	-0.6152	0.1824	-0.9371	-0.4121	0.3766	-0.2297	0.4844	-0.2014	0.2563	0.7237	-0.0767	1.0663	39.2388	59.9527	-54.6714
55	1.0903	-0.6501	-0.0525	3.2423	0.8019	1.143	0.0314	0.3886	0.0235	-0.3366	0.1671	-0.8863	0.0849	0.2717	-0.179	0.3511	-0.0494	0.0457	0.6977	-0.1091	0.511	25.6796	37.9096	-56.8973
56	0.6951	-0.8797	0.2078	8.4848	-0.687	6.1033	0.1245	-0.1786	-0.2241	0.6204	0.0472	0.0252	-1.2811	-0.3113	-0.0404	0.5708	0.0861	-0.0539	0.4823	0.1143	1.1614	45.0436	29.2201	30.2444
57	2.7154	-0.5091	-0.6614	4.6871	-0.3464	4.2788	0.0293	-1.2895	1.1383	1.2105	0.2625	-0.3889	-0.887	0.6356	-0.1365	0.689	-0.054	-0.2484	0.609	-0.261	0.8478	32.5646	27.5806	36.6787
58	3.4768	0.6072	-1.0121	2.3339	-0.3543	1.9528	-0.0016	0.466	-0.1745	-0.1714	-0.0293	-0.1151	0.0408	-0.4805	-0.0187	0.333	0.0339	-0.0268	0.4576	0.0787	0.4565	36.2142	22.5819	25.3213
59	3.0388	-1.6958	0.8714	6.9085	-1.4191	4.516	-0.4599	-0.8249	0.3625	0.8439	0.4894	-0.0355	-0.548	-0.4775	-0.07	0.3323	0.0694	0.0075	0.4592	0.2689	0.5817	29.7649	34.5562	31.9961
60	1.8886	0.3939	-1.0316	0.5991	0.1166	0.7717	-0.222	0.1774	0.0857	0.5043	0.0678	0.5409	-0.2853	-0.4852	0.118	0.3429	0.0734	-0.1541	0.4529	-0.055	0.9599	58.7249	27.6394	24.1906
61	2.557	-2.0421	1.0561	2.8191	-2.7872	3.6617	-0.0583	0.3214	0.6475	-0.5697	-0.2223	0.0018	-0.1799	-0.463	0.4137	0.4635	-0.0889	-0.1291	0.625	0.0176	0.6314	66.8016	54.9619	14.698
62	0.7189	1.4996	-0.1371	5.2399	-0.0386	2.2342	-0.3645	-0.3665	-0.1019	0.2553	0.6303	0.7728	-0.3157	-0.0901	-0.2081	0.2428	0.0377	-0.0054	0.6138	0.0943	0.5857	63.2989	45.02	26.2954
63	2.8808	-0.0559	-0.2411	5.0892	-0.1271	2.0438	0.1496	0.1709	-0.0262	-0.1134	-0.176	-0.1168	0.0364	0.3011	0.0315	0.3203	0.0181	-0.0258	0.4782	0.0197	0.6443	67.1325	46.183	23.2792
64	3.9788	3.2469	2.0978	3.8647	4.0653	5.6506	-0.0256	1.0369	0.6345	-0.7723	0.261	-0.4167	-0.3367	0.0325	-0.1099	0.6573	0.1069	0.1105	0.9935	0.52	0.8831	29.3003	45.5307	13.0403
65	3.8842	-0.2739	-0.4596	2.5192	-0.5098	2.4206	0.261	0.6647	0.2124	-0.0182	0.0789	0.3024	-0.3214	-1.0595	-0.3039	0.4242	0.1714	0.1181	0.8924	0.315	0.8738	23.5999	40.6171	21.6756
66	0.8087	0.5634	-0.75	0.4606	-0.2823	1.1373	0.0765	0.3291	0.6942	-0.0944	0.2227	0.2479	-0.1899	-0.581	-0.2994	0.4076	0.0614	0.0441	0.6301	0.2694	0.9062	29.4643	41.3185	19.5563
67	6.661	-3.0989	0.5419	6.8932	0.1997	4.3461	0.2081	0.6402	-0.1022	-0.6539	-0.2618	0.987	-0.2556	-0.2989	0.0935	0.4142	0.0505	-0.053	0.4866	0.1524	0.5392	58.6435	47.7575	11.5586
68	4.3021	-0.7945	0.9769	4.4809	0.7612	4.5066	0.3623	0.6098	0.6613	-0.2545	0.3032	0.3299	-0.0262	0.58	-0.5817	0.5907	0.0362	0.0532	0.4285	0.0539	0.4934	60.4921	43.3427	9.0835
69	2.506	0.9977	-1.3573	4.6966	1.4855	1.6934	0.2003	0.4854	0.7352	-1.0832	-0.5606	0.8463	-1.0224	0.0372	0.3455	0.851	0.1348	-0.1076	0.9594	0.1323	0.5058	56.8532	50.6526	2.9348
70	3.7224	0.9072	0.0247	3.9918	-1.1541	4.089	-0.1387	-0.2284	-0.1406	-0.4607	-0.1388	0.3889	0.3122	-0.5629	0.2623	0.4015	-0.0801	-0.0863	0.5008	-0.0676	0.8144	60.9031	52.7642	37.6086
71	4.7432	1.3585	-0.536	6.9635	0.2702	2.691	0.1331	-0.5102	-0.4595	0.6604	-0.1502	0.1632	-0.129	-0.5341	-0.0079	0.3354	-0.0861	0.0337	0.5264	-0.0614	0.4642	56.0962	63.5155	43.8527
72	4.3395	-1.6278	3.7015	5.3952	-3.1248	8.9684	0.3315	0.012	0.3316	-0.7545	0.1175	-0.3791	-0.3539	0.3215	-0.2935	0.4537	-0.1087	0.0036	0.3121	-0.0031	0.622	61.5135	64.492	34.645
73	2.7952	-2.9597	2.2311	4.526	-0.7214	3.6566	0.1821	-0.0053	-0.2283	-0.2492	-0.1832	-0.1128	-0.3905	-0.0389	0.0022	0.3223	-0.1121	0.0125	0.5185	-0.0056	0.4248	54.4636	58.4383	41.6982
74	-0.0104	0.0295	-0.0333	1.1808	-0.3394	0.4224	0.2878	-0.6764	-0.6415	0.5647	-0.2479	-0.1117	0.1991	0.055	-0.0561	0.9431	-0.2415	-0.3307	0.9241	0.4639	1.0807	54.963	27.8794	49.5588
75	3.9955	3.7424	-0.5896	4.9648	0.3922	1.5961	0.1076	-0.2685	-0.4343	0.2527	-0.4194	-0.0366	0.1667	-0.0133	-0.1177	0.5837	-0.05	-0.2062	0.6465	0.4706	0.8547	55.4996	27.1838	43.2093
76	0.5659	0.548	-0.7567	2.7189	-0.5283	1.6243	0.3875	-0.9188	-1.1116	0.6821	-0.0079	-0.6236	0.4078	-0.2853	-0.2814	0.633	-0.0884	-0.2964	0.7318	0.3783	0.9239	52.8858	24.1235	44.0442
77	3.4248	0.9947	0.6371	2.5824	0.2318	1.8816	0.1204	-0.2293	-0.5665	0.4954	0.2761	0.4274	-0.173	-0.3924	-0.3191	0.6713	-0.1715	0.3048	0.8499	0.1172	0.925	34.898	56.0523	55.8699
78	2.7671	0.2579	-0.2858	5.1164	2.8537	4.6314	-0.0709	-0.7296	-0.2358	-0.0264	0.1625	-0.0531	-0.6812	0.1294	0.0071	0.4734	0.079	0.0842	0.7272	-0.1359	0.6422	41.1133	65.4077	52.5843
79	9.4265	-0.3492	1.949	3.9007	-4.2277	4.9768	0.1135	-0.4077	-0.2933	0.2667	0.113	0.8716	0.1061	-0.7181	-0.1751	0.4332	-0.0884	0.2102	0.8321	-0.2097	0.7428	34.8416	63.5685	43.607
80	2.1936	0.7762	-0.4787	3.0325	-0.7591	1.3793	-0.089	-0.5059	-0.253	0.2595	-0.085	0.7502	0.0237	-0.587	0.1898	0.4347	-0.0924	0.134	0.731	-0.0588	0.5716	40.5281	65.636	47.535
81	3.2234	0.8281	1.0285	1.582	-0.7684	1.1084	-0.3375	0.2063	0.1967	0.3791	-0.4956	0.1889	0.2485	0.0899	0.316	0.6593	-0.3105	0.4173	1.1326	0.0185	1.5501	35.8764	46.4789	53.8429
82	4.2784	0.3705	0.6194	2.1711	-1.2586	1.1585	0.9172	-1.5035	0.604	1.1219	-0.4719	-0.222	-0.2574	0.4754	-0.3395	1.5469	-0.8291	-0.2655	1.8872	0.2551	0.821	47.9637	30.0285	59.7977
83	0.429	0.8094	0.5811	1.6465	0.7887	2.5809	0.2664	-0.2967	-0.1848	0.6543	-0.2168	0.1468	0.3113	0.1135	-0.0034	0.8137	-0.2745	0.1234	0.8722	0.0326	0.4608	47.9075	34.3166	52.8544
84	1.7308	1.3692	1.0362	1.2058	0.463	1.7383	0.0487	-1.4879	-0.2576	1.5038	-0.3868	-0.052	0.2731	-0.3637	0.2054	1.2221	-0.4011	-0.0994	2.0144	0.4015	0.6947	51.3503	29.0211	63.097
85	1.9606	-0.7863	-1.1447	3.4234	-3.6337	8.3054	0.012	-0.3574	-0.117	0.392	0.2702	0.246	-0.1477	-0.1281	-0.134	1.6744	-0.933	-0.0518	2.0847	0.4666	0.7407	50.0258	21.9607	65.8607
86	1.3036	0.1738	-0.6597	4.8554	2.3308	3.8354	-0.4091	0.0014	-0.068	-0.6341	0.4145	-0.1851	0.9667	-0.1571	-0.0402	1.1008	0.0663	0.0525	0.5596	0.043	0.6426	93.1694	71.8727	-97.0492
87	2.6449	-0.4365	-1.1893	1.6975	0.513	0.6176	0.0362	-0.4612	0.1435	-0.5278	0.0805	-0.2445	0.4905	0.0754	-0.077	1.4899	0.0479	-0.0148	0.7344	0.1479	0.5092	90.9482	60.6039	-90.0671
88	3.2518	-0.1275	-0.1873	4.0739	1.5502	1.0485	0.1509	-0.1889	-0.1145	-0.2042	0.2774	0.7335	-0.3	-0.5701	-0.382	1.2175	-0.1977	0.0423	0.7979	-0.0598	0.447	82.2319	64.9363	-92.9204
89	2.8887	0.4442	-0.4827	2.6942	0.4174	0.9077	-0.383	-0.4323	0.2228	0.395	0.5356	-0.3922	0.6443	0.3706	-0.1759	0.9766	0.0468	-0.1724	0.8451	-0.0562	0.5464	105.7936	71.2545	-93.2769
90	3.4934	-0.3913	-1.4069	1.322	0.6048	2.1369	-0.6971	-0.0608	0.3935	-0.2303	0.6774	-0.2251	0.3628	0.3936	0.0614	0.9533	0.0525	-0.1663	0.7847	-0.3424	0.6856	118.7785	78.3662	-100.2891
91	3.7972	-0.1127	0.8604	2.3235	-0.4952	0.6112	-0.3374	-0.6047	0.1834	0.0232	0.5941	-0.6157	-0.2236	0.5996	-0.2769	1.0895	0.0363	-0.1391	0.7017	-0.2707	0.6083	119.461	76.8242	-93.9983
92	0.9335	-0.1857	-0.1497	1.0172	-0.5712	1.0064	-0.0775	0.2365	-0.1582	-0.1739	-0.1427	-0.0447	0.009	0.2172	0.1266	0.7648	0.0138	0.0646	0.7838	-0.451	0.8063	124.469	83.095	-103.8386
93	2.0894	0.6066	-1.7249	3.2477	0.3348	3.8476	0.8112	0.4766	0.2719	-0.2517	0.3437	-1.2687	-0.1116	0.5459	-0.9491	0.7617	-0.1995	0.0635	0.5959	-0.2529	1.0297	123.2149	88.1067	-101.6561
94	4.4961	1.7457	-4.7756	1.0227	-1.5904	5.2591	0.4434	0.1761	-0.3103	-0.1613	-0.4402	-0.1415	-0.1235	-0.2414	0.0426	1.543	0.2654	-0.055	0.937	0.3298	0.8988	106.8238	47.7993	-81.8946
95	1.5762	-0.0773	-0.6868	0.6951	0.1544	1.1379	-0.3458	-0.2042	-0.9418	0.3395	0.3374	-0.1847	1.1352	-0.2092	-0.0148	1.6921	0.005	0.0913	0.7795	0.2515	0.8752	100.8296	48.9438	-88.5003
96	1.326	0.0088	0.5409	0.632	0.0191	1.9859	-0.2255	-0.0259	-0.0806	1.3174	0.7592	-0.5755	0.563	0.4022	-0.4053	1.3181	0.425	-0.2362	0.8596	-0.2509	0.6976	121.6235	75.9237	-83.6713
97	1.6472	-0.7649	-0.0795	0.452	0.5414	2.6259	-0.1222	-0.1844	-0.0471	0.1984	0.3255	-0.038	0.7377	0.6796	-0.1153	1.7252	0.2529	-0.6429	1.0934	-0.2982	1.1166	127.5834	71.7062	-77.8058
98	2.8087	-0.0903	0.2746	2.2573	0.0559	2.1716	-0.0069	-0.1121	1.0247	-0.2596	0.023	0.1219	-0.1315	0.0456	-0.0147	0.9753	-0.0083	0.1371	0.4506	-0.1255	0.7177	124.4933	66.3149	-118.4666
99	2.2111	0.1754	-0.1796	2.2292	0.2884	0.1616	-0.1083	-0.02	0.45	0.0792	0.0431	-0.4593	0.5512	0.0058	0.0764	0.9168	0.0211	0.183	0.5539	-0.1313	0.6018	121.9388	68.6626	-115.0656
100	3.4936	0.4342	-0.6332	2.3514	0.1711	1.5132	0.1118	-0.1605	-0.0416	-0.0325	-0.1623	0.061	-0.1203	-0.2307	0.0041	0.7789	-0.1284	-0.0935	0.512	-0.0115	0.3801	90.9298	78.3798	-112.5739
101	2.1114	-0.048	-1.5181	0.2462	-0.3565	1.7126	-0.1897	0.3051	-0.4405	-0.5395	-0.1166	0.0131	0.3474	0.0353	0.0271	1.5183	-0.2014	0.2143	0.9846	-0.6062	1.2245	107.6195	46.7188	-126.3012
102	3.0379	-0.9076	0.1857	3.4307	0.2911	2.7365	0.2231	0.7117	-0.6351	-0.2978	-0.5124	-0.0284	0.309	0.4804	0.2651	1.2756	0.0678	0.078	0.6188	-0.1958	0.9065	118.3662	48.0964	-122.4252
103	4.4459	1.4557	-3.5384	2.5053	-0.0835	7.6699	0.131	-0.4878	-0.1938	0.1521	-0.2537	0.0852	0.7648	0.2717	0.1138	1.4487	-0.2437	0.3872	0.5203	-0.2482	1.017	115.3393	41.6821	-112.6501
104	1.4837	-0.5368	0.7065	0.1872	-0.253	0.3386	0.1406	-0.078	-0.9467	-0.8358	0.0065	0.5242	0.046	0.3056	0.0656	1.4583	-0.0906	0.171	0.5314	-0.2538	1.171	114.1494	44.6932	-122.0468
105	2.2197	0.6023	-0.3185	0.8672	-0.2424	1.7106	0.221	-0.0012	-0.248	-0.5455	-0.1586	0.139	0.7993	0.2514	0.0156	1.1811	0.0825	0.1945	0.6426	-0.094	0.7896	122.5919	51.3989	-118.906
106	3.6026	0.1883	0.2564	0.0175	0.1711	3.548	0.0774	-0.1861	-0.3956	-0.1783	-0.1169	0.0788	0.1698	-0.016	0.0447	0.6659	-0.1098	-0.0173	0.8038	-0.0642	0.6558	85.5733	66.2069	-124.4
107	1.7163	-0.0863	-0.9643	0.426	-0.7528	2.0889	-0.365	0.2331	-0.2727	-0.7979	-0.1013	0.2512	0.153	0.2859	0.3849	1.3757	-0.2196	-0.1586	0.7811	-0.0315	0.3914	93.0702	71.892	-128.4786
108	2.8034	-1.0417	0.0125	3.0331	-0.6543	1.8305	0.1743	0.4998	0.1297	0.041	-0.2452	-0.1539	0.2318	-0.7222	0.0527	1.0469	-0.104	-0.0569	0.6249	-0.0183	0.3318	91.0203	73.6421	-126.4972
109	1.0843	-2.2549	-0.0004	9.2962	0.914	1.1792	0.108	0.5532	-0.5006	-0.2078	0.1209	-0.07	0.0746	-0.2554	-0.2477	1.0076	-0.0596	-0.2465	0.9393	-0.1283	0.6723	85.1966	69.3981	-131.9446
110	2.2809	1.2751	-0.9556	1.5446	-1.9459	2.9046	0.3297	0.8577	0.2682	0.0019	-0.0215	0.3539	-0.5311	-0.774	-0.2311	0.683	0.2546	-0.0318	0.7863	0.0891	0.5997	-4.0299	41.8537	-85.4478
111	6.7873	1.53	-0.3493	2.9955	-1.9346	4.7014	0.3735	1.4909	-0.2258	0.0649	0.1414	0.5453	-1.0513	-0.3394	-0.4732	0.6634	0.2594	-0.0143	1.0183	0.1626	0.5888	-8.8284	47.0367	-86.4537
112	3.9414	0.2438	-0.4955	4.6807	-1.7146	5.1447	0.5591	-0.0776	-0.1762	0.6915	-0.5245	-0.3983	-0.32	0.091	-0.0114	0.5602	0.047	-0.0055	0.5854	0.1382	0.5517	0.9658	40.784	-81.7569
113	1.3297	-0.3855	0.2476	1.1767	-0.5303	4.3347	0.4122	0.4591	-0.1927	-0.1599	-0.1454	-0.0578	-0.1781	0.3679	-0.1393	0.1945	-0.0378	-0.135	0.5372	-0.4892	0.8973	24.9722	82.5348	-26.361
114	1.5584	-0.87	-0.6247	2.2687	-0.6716	1.3824	0.1213	0.3021	-0.3703	-0.2397	-0.0556	-0.7861	-0.3053	0.1551	-0.0954	0.3431	0.0978	-0.2057	0.4298	-0.1576	0.6365	34.3863	86.1863	-17.3285
115	4.0563	-2.6041	2.0087	3.4041	-1.7671	2.4435	-0.1703	-1.0391	-0.4592	0.3642	0.1708	0.1426	-0.6005	-0.1215	0.0918	0.3665	-0.003	-0.0484	0.5157	-0.0815	0.5323	24.2932	84.8382	-11.8126
116	6.0116	1.1101	-0.3624	2.3751	0.7952	3.9148	0.5736	-0.1864	-0.0698	-0.1812	0.3523	0.5181	0.2205	-0.2624	-0.7535	0.5057	0.0183	-0.2674	0.4513	0.0275	0.7078	33.0536	76.8022	-13.5638
117	3.2881	-0.8036	1.672	1.8095	-1.9846	2.3898	-0.6904	-0.1659	2.6118	0.3755	-0.0816	-0.4574	-1.1039	0.6062	0.6093	0.4664	0.022	-0.3276	0.8651	-0.1428	1.402	40.6636	82.9024	-23.3858
118	2.1075	-0.5114	1.5074	1.874	-0.3365	1.0766	0.0308	-0.2745	-0.1929	-0.0052	-0.2891	0.5943	0.0088	0.1145	-0.0226	0.0016	0.0773	-0.1521	0.6591	-0.2657	0.9998	20.8804	75.7977	-20.5337
119	5.6733	1.8871	2.2784	1.0237	0.7476	3.6101	0.2003	0.0302	0.0623	0.1996	-0.0187	-0.7365	-0.1025	0.1834	0.3054	-0.0273	-0.1	-0.152	0.4217	-0.2998	0.7545	31.9007	89.0851	-12.5732
120	1.4387	-0.3341	1.0719	1.1259	-0.8354	3.807	-0.3581	-0.6681	0.3041	0.3006	-0.2207	-0.7413	-0.3852	-0.4023	0.3936	0.3079	0.008	-0.2009	0.6177	-0.0184	0.6566	26.9959	87.4095	-12.898
121	0.4336	0.5381	0.0121	1.1882	0.0167	0.5689	0.2126	-0.0317	0.9173	0.5308	-0.1161	-0.0205	0.072	-0.0503	-0.0156	0.4124	0.0683	-0.1345	0.6354	-0.1398	0.6128	-16.5177	148.4658	-44.9146
122	3.4373	2.0561	-0.5448	4.0261	-1.767	2.839	-0.4156	-0.0725	0.356	-0.4484	0.1211	0.3655	-0.0391	-0.3785	0.309	0.2736	0.0183	0.0318	0.4014	-0.0093	0.5555	-10.1383	152.9524	-55.1166
123	2.2374	-0.4758	-2.1576	4.2124	-2.0319	3.5575	-0.2081	0.4157	0.2687	-0.085	0.5939	0.5887	-0.2693	-0.4329	-0.3192	0.3639	0.0127	-0.134	0.3581	0.0371	0.4655	-16.854	154.905	-53.7152
124	1.5279	-0.7227	0.7239	0.3923	-0.0641	1.797	-0.3074	0.164	0.1638	-0.3337	-0.0745	-0.2641	0.0459	0.438	0.3794	0.5658	-0.0377	-0.1152	0.2981	0.0522	0.5979	-8.1121	151.0106	-59.4452
125	4.2357	0.619	-0.1809	0.4311	0.1608	0.9064	0.0737	0.4313	0.0297	-0.7514	0.1392	0.159	0.5342	0.1471	-0.1005	0.6079	-0.098	-0.2051	0.3487	0.0294	0.4764	58.124	16.6097	-25.5471
126	5.0751	-0.3642	1.4773	3.3432	0.1542	5.7064	0.2341	0.3113	0.4939	-0.5845	0.0747	-0.5422	0.8113	1.0775	-0.2717	0.4258	0.0393	-0.0306	0.5608	0.0557	0.5487	67.7194	19.6765	-16.8868
127	3.7793	-1.3098	-0.1122	3.7785	-0.6936	0.1659	-0.0385	-0.9227	-0.3055	-0.0287	0.095	-0.4649	-0.0956	-0.5646	0.076	0.5255	-0.0646	-0.0888	0.6161	0.0359	0.2497	63.7195	17.4018	-10.2672
128	4.0524	-0.649	-0.306	3.3282	1.0966	2.6435	0.1162	-0.1738	-0.5372	-0.128	-0.307	0.4069	-0.3345	0.3579	0.2443	0.3701	0.2053	-0.189	0.6381	0.0174	0.4041	62.1058	10.4363	-13.9501
129	1.321	-1.6104	0.978	4.2814	-0.2802	3.3643	0.5034	0.1619	0.2218	-0.0511	-0.0495	0.2164	0.1796	-0.0021	-0.2801	0.4056	0.1499	-0.2254	0.4041	-0.0414	0.6471	61.6541	12.8001	-20.4879
130	1.1354	0.0802	0.1119	1.8423	-0.4455	3.033	-0.3098	-0.4869	0.0423	0.2861	-0.0958	-0.2172	-0.5075	0.3845	0.2316	0.1951	-0.0289	-0.1258	0.4749	0.0649	0.3921	63.0209	23.0636	-12.8683
131	0.272	-0.4337	1.0554	3.2808	-0.7701	4.4007	0.1928	-0.5231	0.1358	0.4461	-0.0414	-0.9914	-0.4573	0.0396	-0.0982	0.6025	-0.1302	-0.1872	0.475	-0.1937	0.7765	23.4725	86.2688	-45.702
132	1.4991	-0.5042	-0.6239	1.0022	-1.4552	3.6006	-0.2462	0.3037	0.0546	-0.8165	0.3648	-1.2604	-0.6094	0.8783	0.1475	0.7136	-0.1866	0.3177	0.5704	-0.2052	1.0203	29.181	89.9769	-56.6485
133	6.4968	6.4265	-5.1332	6.3961	-5.1018	4.0967	0.0479	0.5836	0.0356	-0.78	0.4756	-1.2587	-0.3836	0.1978	-0.4075	0.9522	-0.0977	0.0586	0.7067	-0.2896	0.6459	25.4211	100.0295	-62.2015
134	1.2072	1.1429	-1.8942	1.0814	-1.7937	2.9785	0.4753	0.4925	0.2566	-0.4444	-0.0297	0.1002	-0.3971	-0.0253	-0.3329	0.6507	-0.1213	0.0659	0.6708	-0.0342	0.6373	14.7283	90.2267	-59.8346
135	0.9595	-1.5161	-0.492	2.421	0.4251	5.9224	-0.0257	-0.2398	0.1179	-0.4041	0.5188	-0.1657	-1.0906	0.7344	-0.4747	0.9426	0.0521	0.3534	0.4195	-0.0352	0.7659	24.2922	97.8311	-53.0877
136	0.6568	-0.4176	-1.2896	5.3296	-1.6657	3.7428	0.1419	0.3886	0.159	0.1607	0.2401	-0.8359	-0.4713	-0.0006	-0.3176	0.654	-0.1606	0.1291	0.4992	0.0269	0.7403	21.9477	91.662	-50.1828
137	2.9436	-2.0084	-0.2826	1.8178	0.2687	0.3536	0.1265	0.0191	0.1634	0.1853	-0.0226	0.1878	-0.0731	-0.0924	0.0367	0.8622	-0.1087	0.4166	0.2817	-0.0831	0.5787	24.1667	82.2152	-51.9956
138	2.4384	-0.0206	1.3963	0.3223	-0.9221	3.3484	-0.2857	0.7134	0.8778	-0.4836	-0.0706	-0.6337	-0.3981	0.6084	0.4104	0.9714	-0.3472	0.1252	0.9952	-0.0189	0.9038	35.0471	91.4042	-61.735
139	1.9178	-0.8073	-0.7	1.4433	-2.1107	5.5821	-0.5128	0.0249	0.439	-0.9975	0.1628	-0.6495	-0.1938	0.3391	0.3503	0.9099	-0.3355	0.0291	0.3889	-0.0132	1.0618	28.5843	85.8196	-59.295
140	5.3963	0.4089	-3.9137	4.2161	3.4241	6.1466	0.8644	0.0728	0.4068	0.1334	0.327	0.0913	-0.3608	0.316	-0.8811	0.4072	-0.0521	-0.0859	0.9419	0.1225	0.801	2.8883	27.3354	29.1786
141	4.7835	2.8018	0.2985	8.6213	1.3463	8.3097	-0.1066	-0.3786	-0.1529	-0.0873	-0.1834	0.009	-0.1223	-0.5525	0.2086	0.5573	-0.1093	-0.0912	0.5752	0.0073	0.2651	18.581	10.3223	33.2299
142	0.6736	0.7708	-0.2128	1.2491	0.5585	1.8236	0.6747	0.6441	-0.2528	-0.3799	-0.2066	1.1705	-0.5964	-1.1465	-0.3526	0.9634	0.3018	-0.3391	0.8924	0.1182	1.2573	-1.8517	19.0482	26.8491
143	5.0928	0.3066	0.3811	4.8179	0.2412	4.1515	-0.1149	0.7983	0.1874	-0.8236	-0.1147	0.463	0.4217	0.2601	0.2727	0.5005	0.0139	-0.0732	0.7445	-0.0446	0.5141	6.0867	13.2961	19.2732
144	1.1185	0.5758	1.7911	5.5761	-0.5341	3.9977	-0.5307	0.0646	-0.4216	0.2504	0.3971	0.2906	-0.1976	-0.5185	0.1097	0.3523	-0.0065	0.0809	0.4815	0.1089	0.554	8.4876	11.4983	27.9096
145	2.1756	-3.633	-1.2974	6.6945	2.2761	0.8924	0.1472	0.0059	0.0362	-0.044	0.1572	0.1095	-0.1715	-0.1031	-0.2093	0.4067	-0.1041	-0.0066	0.8932	0.2507	0.6774	14.5287	18.4676	25.0109
146	0.2908	-0.4838	-0.3252	1.6947	1.1345	0.9376	-0.0193	0.9914	0.5202	-0.453	-0.8	0.4715	-0.2182	-0.1563	0.7729	0.4867	0.1443	-0.1269	0.7678	-0.1116	0.7742	5.0403	21.0269	17.6577
147	1.2854	1.0981	1.3079	2.3956	-0.3937	2.8681	0.5461	-0.2034	0.4882	0.0177	0.0492	-0.0877	-0.1197	-0.6167	-0.4578	0.5627	0.0293	-0.0588	0.463	-0.026	0.5408	50.556	90.3572	42.7678
148	2.0154	0.7887	1.0607	0.7121	1.2849	5.1419	0.177	-0.3499	0.1998	0.2556	-0.4686	0.2922	-0.1629	-1.0212	0.3051	0.2887	0.0123	-0.1816	0.7674	-0.2754	0.4663	49.8756	88.7005	51.9985
149	3.038	0.2916	0.571	3.6873	-0.7752	0.7136	-0.4539	-0.0548	0.3356	1.0339	-0.354	1.0971	0.3031	0.1753	0.6475	0.7833	0.0896	-0.0564	0.6741	-0.1991	0.448	52.1355	84.9284	54.3684
150	0.9566	-0.2764	-1.8367	4.1933	1.173	3.6242	0.0152	0.3414	0.209	0.0018	-0.0354	0.7695	-0.4863	-0.4977	0.0608	0.8052	0.0584	-0.2154	0.8106	-0.3539	0.7593	53.4068	99.8496	56.4074
151	5.2148	1.321	-1.9544	5.624	1.2134	6.1409	0.3107	-0.4811	1.2958	0.2131	-0.0598	0.6151	-1.0224	0.1163	-0.2917	0.5893	-0.0582	0.1315	0.43	-0.1879	0.6748	54.3902	95.7638	47.2172
152	1.9756	1.2937	-0.7161	9.1292	0.147	2.2293	0.0366	-0.5878	0.2205	0.8426	-0.0105	-0.8467	-0.4796	0.4442	-0.0246	0.337	-0.1235	0.0872	0.5425	-0.0865	0.3208	61.8888	85.1726	45.144
153	1.5252	-0.1061	-2.2821	2.1647	1.4009	4.1401	-0.1355	-0.1516	0.3246	0.3715	-0.257	0.6197	-0.3002	-0.6265	0.1556	0.6457	0.365	-0.0693	0.7093	-0.4566	0.5564	46.2244	87.6888	50.1432
154	1.9406	0.194	-0.2013	2.104	1.4202	3.7511	-0.6857	-0.4867	0.4837	0.8794	-0.0336	0.2385	0.1143	-0.6475	0.6084	0.5784	0.2422	0.0484	0.7709	-0.1609	0.6259	48.0345	87.2048	54.5072
155	4.1156	-0.3889	0.2513	1.5652	0.8325	3.9362	-0.6424	0.125	0.1149	-0.7115	-0.2558	0.1586	0.3722	-0.068	0.8618	1.8354	-0.5533	-0.3269	1.0047	0.3305	1.0367	68.054	40.7993	-88.572
156	2.6654	-0.1544	-1.6486	1.8734	-0.7468	2.4505	0.0855	-0.5087	-0.2192	-0.0517	0.291	0.8637	0.1158	-0.3116	-0.334	1.2067	-0.24	0.0002	1.3614	0.6272	1.0546	67.2368	43.7266	-78.7092
157	0.9933	-0.0529	-0.7694	0.8032	-1.3427	3.0147	-0.1546	0.3676	-0.0118	-0.4379	0.0992	0.0473	0.3566	-0.6708	-0.3471	1.4913	-0.4537	-0.1449	0.9904	0.5687	0.7759	67.5149	44.7401	-86.1294
158	2.876	0.0523	-1.3334	0.9005	-1.4118	3.9709	-0.3203	-0.7049	-0.4809	-0.6499	0.3383	-0.5037	0.9637	0.1573	0.0423	1.4447	-0.3953	0.0901	0.984	0.3322	0.8755	73.8378	40.4619	-78.1139
159	2.9852	-0.9489	-0.2727	0.3037	-0.0051	3.0908	0.2867	-0.066	0.2985	0.0821	0.1255	0.2438	0.5443	0.6813	-0.4292	1.1956	0.2529	0.1329	0.6831	-0.0393	1.089	146.8568	47.1709	-117.1787
160	2.0017	-0.4935	-0.3285	1.1079	0.7989	0.7796	0.1528	-0.3711	-0.5821	1.0753	0.3059	-0.1091	0.5267	0.089	-0.119	2.2271	0.1722	0.0523	0.4091	0.0371	1.1871	146.2428	36.4074	-127.5412
161	0.2561	-0.3651	-0.5689	1.2409	1.2517	1.5391	0.2613	0.3008	-0.3846	0.0044	-0.3306	0.3887	-0.0223	-0.2092	-0.0208	1.4232	0.183	0.498	0.4665	-0.0121	1.2838	141.6864	46.5379	-130.7552
162	2.3725	-0.4267	0.036	2.8741	0.9085	2.4213	0.4169	-0.1641	0.5624	0.1752	0.3896	0.0135	-0.4937	-0.0228	-0.6652	1.0287	0.1849	0.1693	0.7904	-0.143	1.0529	153.6491	45.1755	-130.4508
163	6.6243	-0.5293	0.2993	0.093	0.4524	4.3482	0.1894	-0.2807	0.3007	-0.4369	0.3972	-0.1334	0.4865	0.2593	-0.3896	1.0106	0.1529	-0.1568	0.6126	-0.1073	1.64	150.4894	47.9806	-121.8951
164	2.7716	-2.1877	-0.6454	2.8826	1.5567	1.9401	-0.0525	-0.1776	-0.2192	0.7239	0.3267	0.1552	0.8153	0.0351	-0.0366	1.459	0.2257	0.2835	0.2127	-0.0031	0.6686	141.9763	40.5705	-125.5644
165	0.1361	0.0803	0.2125	0.7188	0.9064	1.2556	-0.1411	0.0049	0.161	0.0757	-0.3218	0.0068	0.9049	-0.1721	0.3434	1.3772	0.2158	0.3497	0.585	-0.0969	0.8652	141.7397	40.1796	-131.2012

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 5 through 23 )	A	5 - 23
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 24 through 48 )	A	24 - 48
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 49 through 75 )	A	49 - 75
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 76 through 91 )	A	76 - 91
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 92 through 115 )	A	92 - 115
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 116 through 128 )	A	116 - 128
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 129 through 150 )	A	129 - 150
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 151 through 172 )	A	151 - 172
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 173 through 187 )	A	173 - 187
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 188 through 198 )	A	188 - 198
11	X-RAY DIFFRACTION	11	chain 'A' and (resid 199 through 211 )	A	199 - 211
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 1 through 40 )	B	1 - 40
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 41 through 67 )	B	41 - 67
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 71 through 111 )	B	71 - 111
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 114 through 186 )	B	114 - 186
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 201 through 211 )	B	201 - 211
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 5 through 91 )	C	5 - 91
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 92 through 113 )	C	92 - 113
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 114 through 150 )	C	114 - 150
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 151 through 163 )	C	151 - 163
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 164 through 213 )	C	164 - 213
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 1 through 17 )	D	1 - 17
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 18 through 44 )	D	18 - 44
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 45 through 64 )	D	45 - 64
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 65 through 113 )	D	65 - 113
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 114 through 141 )	D	114 - 141
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 142 through 164 )	D	142 - 164
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 165 through 182 )	D	165 - 182
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 183 through 189 )	D	183 - 189
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 190 through 215 )	D	190 - 215
31	X-RAY DIFFRACTION	31	chain 'E' and (resid 5 through 24 )	E	5 - 24
32	X-RAY DIFFRACTION	32	chain 'E' and (resid 25 through 49 )	E	25 - 49
33	X-RAY DIFFRACTION	33	chain 'E' and (resid 50 through 114 )	E	50 - 114
34	X-RAY DIFFRACTION	34	chain 'E' and (resid 115 through 151 )	E	115 - 151
35	X-RAY DIFFRACTION	35	chain 'E' and (resid 152 through 175 )	E	152 - 175
36	X-RAY DIFFRACTION	36	chain 'E' and (resid 176 through 213 )	E	176 - 213
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 1 through 39 )	F	1 - 39
38	X-RAY DIFFRACTION	38	chain 'F' and (resid 40 through 66 )	F	40 - 66
39	X-RAY DIFFRACTION	39	chain 'F' and (resid 67 through 116 )	F	67 - 116
40	X-RAY DIFFRACTION	40	chain 'F' and (resid 117 through 141 )	F	117 - 141
41	X-RAY DIFFRACTION	41	chain 'F' and (resid 142 through 216 )	F	142 - 216
42	X-RAY DIFFRACTION	42	chain 'G' and (resid 5 through 24 )	G	5 - 24
43	X-RAY DIFFRACTION	43	chain 'G' and (resid 25 through 49 )	G	25 - 49
44	X-RAY DIFFRACTION	44	chain 'G' and (resid 50 through 114 )	G	50 - 114
45	X-RAY DIFFRACTION	45	chain 'G' and (resid 115 through 213 )	G	115 - 213
46	X-RAY DIFFRACTION	46	chain 'H' and (resid 1 through 17 )	H	1 - 17
47	X-RAY DIFFRACTION	47	chain 'H' and (resid 18 through 50 )	H	18 - 50
48	X-RAY DIFFRACTION	48	chain 'H' and (resid 51 through 63 )	H	51 - 63
49	X-RAY DIFFRACTION	49	chain 'H' and (resid 64 through 126 )	H	64 - 126
50	X-RAY DIFFRACTION	50	chain 'H' and (resid 127 through 142 )	H	127 - 142
51	X-RAY DIFFRACTION	51	chain 'H' and (resid 143 through 214 )	H	143 - 214
52	X-RAY DIFFRACTION	52	chain 'I' and (resid 5 through 113 )	I	5 - 113
53	X-RAY DIFFRACTION	53	chain 'I' and (resid 114 through 213 )	I	114 - 213
54	X-RAY DIFFRACTION	54	chain 'J' and (resid 1 through 86 )	J	1 - 86
55	X-RAY DIFFRACTION	55	chain 'J' and (resid 87 through 214 )	J	87 - 214
56	X-RAY DIFFRACTION	56	chain 'K' and (resid 2 through 19 )	K	2 - 19
57	X-RAY DIFFRACTION	57	chain 'K' and (resid 20 through 34 )	K	20 - 34
58	X-RAY DIFFRACTION	58	chain 'K' and (resid 35 through 90 )	K	35 - 90
59	X-RAY DIFFRACTION	59	chain 'K' and (resid 91 through 102 )	K	91 - 102
60	X-RAY DIFFRACTION	60	chain 'K' and (resid 103 through 114 )	K	103 - 114
61	X-RAY DIFFRACTION	61	chain 'K' and (resid 115 through 129 )	K	115 - 129
62	X-RAY DIFFRACTION	62	chain 'K' and (resid 130 through 164 )	K	130 - 164
63	X-RAY DIFFRACTION	63	chain 'K' and (resid 165 through 213 )	K	165 - 213
64	X-RAY DIFFRACTION	64	chain 'L' and (resid 1 through 17 )	L	1 - 17
65	X-RAY DIFFRACTION	65	chain 'L' and (resid 18 through 67 )	L	18 - 67
66	X-RAY DIFFRACTION	66	chain 'L' and (resid 68 through 126 )	L	68 - 126
67	X-RAY DIFFRACTION	67	chain 'L' and (resid 127 through 152 )	L	127 - 152
68	X-RAY DIFFRACTION	68	chain 'L' and (resid 153 through 195 )	L	153 - 195
69	X-RAY DIFFRACTION	69	chain 'L' and (resid 196 through 214 )	L	196 - 214
70	X-RAY DIFFRACTION	70	chain 'M' and (resid 5 through 24 )	M	5 - 24
71	X-RAY DIFFRACTION	71	chain 'M' and (resid 25 through 62 )	M	25 - 62
72	X-RAY DIFFRACTION	72	chain 'M' and (resid 63 through 76 )	M	63 - 76
73	X-RAY DIFFRACTION	73	chain 'M' and (resid 77 through 102 )	M	77 - 102
74	X-RAY DIFFRACTION	74	chain 'M' and (resid 103 through 129 )	M	103 - 129
75	X-RAY DIFFRACTION	75	chain 'M' and (resid 130 through 151 )	M	130 - 151
76	X-RAY DIFFRACTION	76	chain 'M' and (resid 152 through 211 )	M	152 - 211
77	X-RAY DIFFRACTION	77	chain 'N' and (resid 1 through 20 )	N	1 - 20
78	X-RAY DIFFRACTION	78	chain 'N' and (resid 21 through 42 )	N	21 - 42
79	X-RAY DIFFRACTION	79	chain 'N' and (resid 43 through 70 )	N	43 - 70
80	X-RAY DIFFRACTION	80	chain 'N' and (resid 71 through 103 )	N	71 - 103
81	X-RAY DIFFRACTION	81	chain 'N' and (resid 104 through 116 )	N	104 - 116
82	X-RAY DIFFRACTION	82	chain 'N' and (resid 117 through 159 )	N	117 - 159
83	X-RAY DIFFRACTION	83	chain 'N' and (resid 160 through 175 )	N	160 - 175
84	X-RAY DIFFRACTION	84	chain 'N' and (resid 176 through 206 )	N	176 - 206
85	X-RAY DIFFRACTION	85	chain 'N' and (resid 207 through 213 )	N	207 - 213
86	X-RAY DIFFRACTION	86	chain 'O' and (resid 5 through 23 )	O	5 - 23
87	X-RAY DIFFRACTION	87	chain 'O' and (resid 29 through 49 )	O	29 - 49
88	X-RAY DIFFRACTION	88	chain 'O' and (resid 50 through 76 )	O	50 - 76
89	X-RAY DIFFRACTION	89	chain 'O' and (resid 77 through 129 )	O	77 - 129
90	X-RAY DIFFRACTION	90	chain 'O' and (resid 130 through 164 )	O	130 - 164
91	X-RAY DIFFRACTION	91	chain 'O' and (resid 165 through 187 )	O	165 - 187
92	X-RAY DIFFRACTION	92	chain 'O' and (resid 188 through 199 )	O	188 - 199
93	X-RAY DIFFRACTION	93	chain 'O' and (resid 200 through 211 )	O	200 - 211
94	X-RAY DIFFRACTION	94	chain 'P' and (resid 0 through 19 )	P	0 - 19
95	X-RAY DIFFRACTION	95	chain 'P' and (resid 20 through 126 )	P	20 - 126
96	X-RAY DIFFRACTION	96	chain 'P' and (resid 134 through 195 )	P	134 - 195
97	X-RAY DIFFRACTION	97	chain 'P' and (resid 196 through 214 )	P	196 - 214
98	X-RAY DIFFRACTION	98	chain 'Q' and (resid 5 through 62 )	Q	5 - 62
99	X-RAY DIFFRACTION	99	chain 'Q' and (resid 63 through 114 )	Q	63 - 114
100	X-RAY DIFFRACTION	100	chain 'Q' and (resid 115 through 213 )	Q	115 - 213
101	X-RAY DIFFRACTION	101	chain 'R' and (resid 1 through 19 )	R	1 - 19
102	X-RAY DIFFRACTION	102	chain 'R' and (resid 20 through 51 )	R	20 - 51
103	X-RAY DIFFRACTION	103	chain 'R' and (resid 52 through 67 )	R	52 - 67
104	X-RAY DIFFRACTION	104	chain 'R' and (resid 71 through 98 )	R	71 - 98
105	X-RAY DIFFRACTION	105	chain 'R' and (resid 99 through 113 )	R	99 - 113
106	X-RAY DIFFRACTION	106	chain 'R' and (resid 114 through 126 )	R	114 - 126
107	X-RAY DIFFRACTION	107	chain 'R' and (resid 135 through 164 )	R	135 - 164
108	X-RAY DIFFRACTION	108	chain 'R' and (resid 165 through 200 )	R	165 - 200
109	X-RAY DIFFRACTION	109	chain 'R' and (resid 201 through 214 )	R	201 - 214
110	X-RAY DIFFRACTION	110	chain 'a' and (resid 3 through 59 )	a	3 - 59
111	X-RAY DIFFRACTION	111	chain 'a' and (resid 60 through 87 )	a	60 - 87
112	X-RAY DIFFRACTION	112	chain 'a' and (resid 88 through 113 )	a	88 - 113
113	X-RAY DIFFRACTION	113	chain 'c' and (resid 3 through 17 )	c	3 - 17
114	X-RAY DIFFRACTION	114	chain 'c' and (resid 18 through 39 )	c	18 - 39
115	X-RAY DIFFRACTION	115	chain 'c' and (resid 40 through 52 )	c	40 - 52
116	X-RAY DIFFRACTION	116	chain 'c' and (resid 53 through 72 )	c	53 - 72
117	X-RAY DIFFRACTION	117	chain 'c' and (resid 73 through 79 )	c	73 - 79
118	X-RAY DIFFRACTION	118	chain 'c' and (resid 80 through 87 )	c	80 - 87
119	X-RAY DIFFRACTION	119	chain 'c' and (resid 88 through 99 )	c	88 - 99
120	X-RAY DIFFRACTION	120	chain 'c' and (resid 100 through 113 )	c	100 - 113
121	X-RAY DIFFRACTION	121	chain 'e' and (resid 3 through 17 )	e	3 - 17
122	X-RAY DIFFRACTION	122	chain 'e' and (resid 18 through 52 )	e	18 - 52
123	X-RAY DIFFRACTION	123	chain 'e' and (resid 53 through 95 )	e	53 - 95
124	X-RAY DIFFRACTION	124	chain 'e' and (resid 96 through 112 )	e	96 - 112
125	X-RAY DIFFRACTION	125	chain 'g' and (resid 3 through 17 )	g	3 - 17
126	X-RAY DIFFRACTION	126	chain 'g' and (resid 18 through 39 )	g	18 - 39
127	X-RAY DIFFRACTION	127	chain 'g' and (resid 40 through 59 )	g	40 - 59
128	X-RAY DIFFRACTION	128	chain 'g' and (resid 60 through 72 )	g	60 - 72
129	X-RAY DIFFRACTION	129	chain 'g' and (resid 73 through 87 )	g	73 - 87
130	X-RAY DIFFRACTION	130	chain 'g' and (resid 88 through 113 )	g	88 - 113
131	X-RAY DIFFRACTION	131	chain 'i' and (resid 3 through 17 )	i	3 - 17
132	X-RAY DIFFRACTION	132	chain 'i' and (resid 18 through 52 )	i	18 - 52
133	X-RAY DIFFRACTION	133	chain 'i' and (resid 53 through 59 )	i	53 - 59
134	X-RAY DIFFRACTION	134	chain 'i' and (resid 60 through 66 )	i	60 - 66
135	X-RAY DIFFRACTION	135	chain 'i' and (resid 67 through 72 )	i	67 - 72
136	X-RAY DIFFRACTION	136	chain 'i' and (resid 73 through 87 )	i	73 - 87
137	X-RAY DIFFRACTION	137	chain 'i' and (resid 88 through 90 )	i	88 - 90
138	X-RAY DIFFRACTION	138	chain 'i' and (resid 91 through 99 )	i	91 - 99
139	X-RAY DIFFRACTION	139	chain 'i' and (resid 100 through 113 )	i	100 - 113
140	X-RAY DIFFRACTION	140	chain 'k' and (resid 1 through 7 )	k	1 - 7
141	X-RAY DIFFRACTION	141	chain 'k' and (resid 8 through 17 )	k	8 - 17
142	X-RAY DIFFRACTION	142	chain 'k' and (resid 18 through 32 )	k	18 - 32
143	X-RAY DIFFRACTION	143	chain 'k' and (resid 33 through 72 )	k	33 - 72
144	X-RAY DIFFRACTION	144	chain 'k' and (resid 73 through 87 )	k	73 - 87
145	X-RAY DIFFRACTION	145	chain 'k' and (resid 88 through 90 )	k	88 - 90
146	X-RAY DIFFRACTION	146	chain 'k' and (resid 91 through 112 )	k	91 - 112
147	X-RAY DIFFRACTION	147	chain 'm' and (resid 3 through 32 )	m	3 - 32
148	X-RAY DIFFRACTION	148	chain 'm' and (resid 33 through 39 )	m	33 - 39
149	X-RAY DIFFRACTION	149	chain 'm' and (resid 40 through 52 )	m	40 - 52
150	X-RAY DIFFRACTION	150	chain 'm' and (resid 53 through 59 )	m	53 - 59
151	X-RAY DIFFRACTION	151	chain 'm' and (resid 60 through 79 )	m	60 - 79
152	X-RAY DIFFRACTION	152	chain 'm' and (resid 80 through 87 )	m	80 - 87
153	X-RAY DIFFRACTION	153	chain 'm' and (resid 88 through 96 )	m	88 - 96
154	X-RAY DIFFRACTION	154	chain 'm' and (resid 97 through 113 )	m	97 - 113
155	X-RAY DIFFRACTION	155	chain 'o' and (resid 3 through 32 )	o	3 - 32
156	X-RAY DIFFRACTION	156	chain 'o' and (resid 33 through 72 )	o	33 - 72
157	X-RAY DIFFRACTION	157	chain 'o' and (resid 73 through 95 )	o	73 - 95
158	X-RAY DIFFRACTION	158	chain 'o' and (resid 96 through 113 )	o	96 - 113
159	X-RAY DIFFRACTION	159	chain 'q' and (resid 3 through 24 )	q	3 - 24
160	X-RAY DIFFRACTION	160	chain 'q' and (resid 25 through 39 )	q	25 - 39
161	X-RAY DIFFRACTION	161	chain 'q' and (resid 40 through 52 )	q	40 - 52
162	X-RAY DIFFRACTION	162	chain 'q' and (resid 53 through 72 )	q	53 - 72
163	X-RAY DIFFRACTION	163	chain 'q' and (resid 73 through 87 )	q	73 - 87
164	X-RAY DIFFRACTION	164	chain 'q' and (resid 88 through 96 )	q	88 - 96
165	X-RAY DIFFRACTION	165	chain 'q' and (resid 97 through 111 )	q	97 - 111