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- PDB-7rp0: Structural Snapshots of Intermediates in the Gating of a K+ Channel -
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Open data
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Basic information
Entry | Database: PDB / ID: 7rp0 | ||||||
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Title | Structural Snapshots of Intermediates in the Gating of a K+ Channel | ||||||
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![]() | TRANSPORT PROTEIN / Ion channel / KcsA | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Reddi, R. / Matulef, K. / Riederer, E.A. / Valiyaveetil, F.I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structures of Gating Intermediates in a K + channell. Authors: Reddi, R. / Matulef, K. / Riederer, E. / Moenne-Loccoz, P. / Valiyaveetil, F.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 120.3 KB | Display | ![]() |
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PDB format | ![]() | 89.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7m2hC ![]() 7m2iC ![]() 7m2jC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 10896.655 Da / Num. of mol.: 1 / Fragment: UNP residues 22-124 / Mutation: Y82A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Non-polymers , 5 types, 44 molecules ![](data/chem/img/F09.gif)
![](data/chem/img/K.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/K.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-F09 / | ||||||
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#5: Chemical | ChemComp-K / #6: Chemical | ChemComp-TBA / | #7: Chemical | ChemComp-DGA / | #8: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.98 Å3/Da / Density % sol: 69.12 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.25 Details: 50 mM MES, pH 6.25, 50 mM magnesium acetate, 27% PEG400 PH range: 6.0-6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 20, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.000032 Å / Relative weight: 1 |
Reflection | Resolution: 2.478→54.98 Å / Num. obs: 58647 / % possible obs: 99.9 % / Redundancy: 7.4 % / CC1/2: 0.99 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 2.48→2.58 Å / Num. unique obs: 3643 / CC1/2: 0.79 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1K4C Resolution: 2.48→54.917 Å / SU ML: 0.35 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 31.59 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 106.83 Å2 / Biso mean: 55.3944 Å2 / Biso min: 19.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.48→54.917 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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